Differential associations between systemic markers of disease and white matter tissue health in middle-aged and older adults

Abstract

Age-associated cerebrovascular disease impacts brain tissue integrity, but other factors, including normal variation in blood markers of systemic health, may also influence the structural integrity of the brain. This cross-sectional study included 139 individuals between 40 to 86 years old who were physically healthy and cognitively intact. Eleven markers (total-cholesterol, high-density lipoprotein, low-density lipoprotein, triglyceride, insulin, fasting glucose, glycated hemoglobin, creatinine, blood urea nitrogen, albumin, total protein) and five derived indicators (estimated glomerular filtration rate, creatinine clearance rate, insulin-resistance, average glucose, and cholesterol/high-density lipoprotein ratio) were obtained from blood sampling. Diffusion tensor imaging was used to evaluate white matter tissue health. Blood markers were clustered into five factors. The first factor (defined as insulin/high-density lipoprotein factor) was associated with markers of integrity in the deep white matter and projection fiber systems, while the third factor (defined as kidney function factor) was associated with different markers of integrity in the periventricular and watershed white matter regions. Differential segregated associations for insulin and high-density lipoprotein levels and serum markers of kidney function may provide information about distinct mechanisms of brain changes across the lifespan. These results emphasize the need to determine whether therapeutic modulation of systemic health and organ function may prevent decline in brain structural integrity.

Keywords

Diffusion-weighted imaging factor analysis insulin kidney white matter

Introduction

Major insights in basic and clinical neuroscience have to great degree come through a perspective of the brain as a unique and somewhat independent organ from the more general health of the organism, with the idea that general health may only contribute to neural health in the case of disease. However, it is likely that normal variation in systemic physiology and end-organ function may contribute to overall brain health. Hints that normal inter-individual variation in peripheral and organ health may contribute to neural integrity have recently appeared in the literature demonstrating that variation in blood pressure is associated with brain tissue health in middle-aged and older individuals even in low-risk individuals and younger cohorts.^1–3

Aside from a focus on the vascular system, very little is known about how normal variation in somatic physiological processes may impact brain health. Standard blood processing panels include markers of endocrine, cardiovascular, kidney, and hematologic dysfunction and ‘out of range’ values are indicative of disorders such as diabetes, metabolic syndrome, and cardiovascular disease, conditions that are all highly prevalent in older adults. Evidence of an important link between general health markers and cognition in non-disease populations comes from studies on the variation in insulin resistance and its related blood markers,^4,5 as well as in glomerular filtration rate (GFR) and kidney filtration substances,^6,7 and suggests that metabolic health markers identified in a simple blood test may also be related to brain health.

Our hypothesis was that physiological alterations in blood markers were associated with brain white matter integrity in normal healthy controls as measured with diffusion tensor imaging (DTI). This has been reported previously in patients with type 2 diabetes in whom regional alterations in regional DTI measures were found to be negatively correlated with HBA_1c, fasting glucose, and serum creatinine.⁸ In patients with end-stage renal disease (ESRD), increased serum urea levels were associated with altered white matter integrity.⁹ In both cases, worse cognitive performance was associated with reduced white matter integrity.^10,11 Recent work has also demonstrated an association between white matter integrity and insulin resistance,¹² serum cholesterol levels,¹³ and kidney function¹⁴ in individuals generally considered disease-free. The current work is much broader in scope examining several putative markers of systemic health. A factor analytic approach allows us to demonstrate the factor structure of the various markers examined and elucidate differential patterns of association among these physiologically and statistically distinct factors. This factor analysis is completely data driven, and there is no prior hypothesis as opposed to in our earlier studies in the same sample on insulin resistance¹² and in a different sample on serum cholesterol levels.¹³ The current study demonstrates that several markers in fact correlate with insulin resistance to form a measure of general ‘metabolic’ health in the normal reference range which is strongly related to indices of white matter integrity.

Materials and methods

Study design and participants

A sample of 250 cognitively healthy middle-aged and older adults was recruited through the Massachusetts General Hospital, the local community, and local senior centers. These individuals form part of a longitudinal cohort to evaluate vascular contributions to brain aging. A total of 139 middle-aged and older adults (56 men/83 women) aged 40–86 years was selected for this cross-sectional study based on the availability of fasting venous blood sample and diffusion-weighted imaging MRI data. All datasets used were acquired within a span of three years. All participants were physically healthy, cognitively intact, and literate with at least a high school education. Participants were excluded if they had major neurologic or psychiatric illnesses, history of stroke, significant head trauma, brain surgery or substance abuse, unstable medical illness, cancer within the nervous system or contraindication for MRI scan. Participants with controlled hypertension, dyslipidemia, or type 2 diabetes were not excluded. One hundred twenty-five participants were Caucasian (89.93%), 12 were African American (8.63%), and two were Asian.

Standard protocol approvals, registrations, and patient consents

The study was approved (#2008P001486/MGH) by the Partners Healthcare Internal Review Board and followed the Ethical Principles and Guidelines for the Protection of Human Subjects of Research, generally known as the Belmont Report. All participants provided written informed consent to participate in this research.

Clinical procedures

Assessments included ascertainment of medical history as well as general medical, physical, and neurologic examinations. Overnight fasting venous blood samples were collected on the day of the MRI session for estimation of 11 markers: total-cholesterol, high-density lipoprotein (HDL), low-density lipoprotein (LDL), triglyceride, fasting serum insulin, fasting glucose, glycosylated hemoglobin A_1C (HbA_1C), creatinine, blood urea nitrogen (BUN), albumin, and total protein. Serum insulin was measured using electro-chemiluminescence immunoassay (Mayo Medical Laboratories, Andover, MA). Five indicators of systemic health – homeostasis model assessment of insulin resistance (HOMA-IR), average glucose level, estimated glomerular filtration rate (eGFR), creatinine clearance (CCL) and cholesterol to HDL ratio (Chol/HDL ratio) – were calculated and will be further referenced in the text as blood markers. The following formulas were used:

HOMA - IR = (fastingglucose \times insulin) / 405

(1)

Averageglucoselevel = (28.7 \times Hb A_{1 C}) - 46.7

(2)

eGFR (chronickidneydisease (CKD) - EPIformul a^{15}) = 141 \times min (Scr / κ, 1)^{α} \times max (Scr / κ, 1)^{- 1.209} \times 0 . 993^{Age} \times 1.018 (ifwoman) \times 1.159 (ifAfricanAmerican)

(3)

where Scr is serum creatinine (mg/dL), κ is a constant with 0.7 for women and 0.9 for men, α is – 0.329 for women and – 0.411 for men, min(Scr/κ,1) indicates the minimum of Scr/κ or 1, and max(Scr/κ,1) indicates the maximum of Scr/κ or 1.

CCL = 0.85 (forwomen) \times ((140 - ageinyears) \times (weightinkg)) / (72 \times Scr)

(4)

Factor analysis of blood markers

Factor analysis was performed to identify the latent structure of the blood markers. The resulting factors were deemed significant based on eigenvalue criteria (>1.0). Varimax orthogonal rotation was used and variables with absolute factor loadings at 0.40 or above were considered major contributors to a factor. Secondary confirmatory analyses included factor analyses without component rotation and without indicators including age in their calculation.

DTI acquisition and processing

MRI studies were performed on a 3 Tesla scanner (Siemens Trio, Erlangen, Germany). The diffusion-weighted images were obtained using single-shot echo planar imaging with a twice-refocused spin-echo sequence:¹⁶ 64 slices, repetition time/echo time = 7980/83 ms, 2 mm isotropic voxels with no slice gap, 60 directions at b = 700 s/mm² with 10 volumes at b = 0 s/mm², acquisition matrix = 128 × 128, flip angle = 90°, and total acquisition time = 8 min, 38 s. Data were preprocessed using the FSL Diffusion Toolbox (http://www.fmrib.ox.ac.uk/fsl). Diffusion volumes were eddy current and motion corrected with affine registration to the non-diffusion weighted volumes (b = 0 s/mm²). The diffusion tensor model was fit to the preprocessed DTI data, and the measures of white matter microstructure – fractional anisotropy (FA), mean diffusivity, axial diffusivity and radial diffusivity – were obtained for each individual. FA maps were registered and aligned into a template space using non-linear registration as part of the FSL Tract-based spatial statistics (TBSS) procedure.¹⁷ A mean FA skeleton representing the centers of all tracts common to the group was computed and used to reduce partial-volume effects in the statistical analyses. Other DTI maps were also registered to the mean FA skeleton.

Statistical analyses

Voxel-wise general linear models (GLMs) were performed using FSL to examine regional associations between each of the significant factors and diffusion measures regressing out the following potential confounders: age, sex, the other factors, and motion parameters (translation and rotation¹⁸). Due to the long scanning period, we also investigated the effect of scanning date. While there was a significant relationship between scanning date and some blood markers and diffusion measures, these associations were independent of the primary associations found between factor scores and diffusion measures and did not affect any of the study findings when added to the models (not shown). Results were corrected for multiple comparisons voxel-wise and threshold-free cluster enhancement¹⁹ were applied to obtain statistical maps with corrected p-value < 0.05. The results were presented on the MNI152 template and expanded for visualization. The resulting significant clusters from the skeleton-restricted analysis were deprojected into each participant's native diffusion space as regions-of-interest (ROI) using the inverse transform created by TBSS. Average measures of white matter microstructure were extracted from these ROIs in native space and were used for (1) visualization of the data distribution in regions showing significance and (2) to examine whether associations were preserved in secondary factor analyses and secondary statistical models. Additional analyses were performed with ‘surrogate variables’ which had the highest loading of each factor and were considered representative of each factor. General extreme studentized deviate with α = 0.05 was used to detect outliers of independent variables, and results excluding outliers were also presented.

Results

Participants

Table 1 presents demographics and average blood markers for all participants. The group average for each blood marker was within the normative values, except for CCL which was slightly lower than normal. However, CCL is known to decrease with age, and our sample includes both middle-aged and older adults.

Table 1.

Demographics, sample averages and reference values of blood markers and indicators.

Variables	Reference values^a	Mean (standard deviation)	Range
Age, y		61.98 (11.22)	40–86
Female, % (n)		59.71 (83)
Body mass index (kg/m²)		26.95 (5.62)	16–48
Education (years)		16.38 (2.56)	12–23
MMSE^b		28.68 (1.40)	24–30
APOE ε4 carriers, % (n)^c		24.07 (26)
Systolic blood pressure		122.32 (14.42)	96–172
Diastolic blood pressure		77.47 (9.95)	50–112
Type 2 diabetes, % (n)^b		13.77 (19)
Antihypertensive treatment, % (n)^b		30.43 (42)
Cholesterol-lowering agent, % (n)^b		28.26 (39)
Cholesterol, mg/dL	<200	187.05 (36.37)	106–313
HDL, mg/dL	40 to 60	59.53 (17.78)	27–116
LDL, mg/dL	<130	107.16 (30.92)	42–204
Triglycerides, mg/dL	<150	101.86 (54.69)	34–313
Chol/HDL ratio	≤5	3.37 (1.03)	1.7–6.6
Average glucose, mg/dL	77 to 137	115.36 (19.39)	91–217
HbA_1C, %	4.3 to 6.4	5.65 (0.68)	4.8–9.2
Insulin, uIU/mL	2.6 to 25.0	8.74 (5.73)	1.8–33
Fasting glucose, mg/dL	70 to 110	91.09 (20.05)	52–200
HOMA-IR	<2.5	2.04 (1.55)	0.33–8.39
Albumin, g/dL	3.3 to 5.0	4.40 (0.23)	3.8–4.9
BUN, mg/dL	8 to 25	16.54 (6.66)	7–64
Creatinine, mg/dL	0.6 to 1.5	0.94 (0.27)	0.58–2.14
eGFR, mL/min/1.73 m²	≥60	77.89 (17.33)	26.2–107.2
CCL, mL/min	97 to 137 for men, 88 to 128 for women	86.21 (31.05)	29.5–109.1
Total protein, g/dL	6.0 to 8.3	7.23 (0.39)	6.2–8.3

MMSE: mini-mental state examination; HDL: high-density lipoprotein; LDL: low-density lipoprotein; Chol: cholesterol; HbA_1C: glycated hemoglobin; HOMA-IR: homeostatic model assessment of insulin resistance; BUN: blood urea nitrogen; eGFR: estimated glomerulus filtration rate; CCL: creatinine clearance.

From Massachusetts General Hospital core laboratory reference ranges (http://mghlabtest.partners.org/MGH-Core-Laboratory-Reference-Ranges.pdf) and the National Cholesterol Education Program²⁰; these values are provided for informational purpose only and may vary based on clinical practice, ethnicity, age, sex, and other factors.

Information missing for one participant.

Information missing for 31 participants.

Factor analysis of blood markers

Five significant primary factors were extracted from the 16 blood markers (Table 2). Factor 1, defined as the ‘insulin/HDL factor (IHF)’, increased with greater fasting insulin, Chol/HDL ratio, HOMA-IR, triglyceride, CCL, and lower HDL. Factor 2, defined as a ‘glucose regulation factor’, increased with greater HOMA-IR, common glucose, HbA_1C, and fasting glucose. Factor 3, defined as a ‘kidney function factor (KFF)’, increased with greater creatinine and BUN, and lower eGFR and CCL. Factor 4, defined as a ‘lipid factor’, increased with greater Chol/HDL ratio, cholesterol, and LDL. Factor 5, defined as a ‘protein factor’, increased with total protein and albumin. With the exception of Factor 5, greater factor scores related to marker levels commonly linked to poorer health or greater risk of disease overall. These five factors cumulatively accounted for 79.42% of the variance of the overall variables and were used in the voxel-wise GLMs with diffusion measures of white matter microstructure.

Table 2.

Primary factor analysis of blood markers and indicators.

Factor	1	2	3	4	5
Interpretation	Insulin/HDL	Glucose	Kidney function	Lipid	Protein
% Variance explained	20.78	18.42	18.03	13.78	8.41
Insulin	0.82	0.19	−0.01	−0.08	0.06
HDL	−0.81	0.01	0.00	0.19	0.02
Chol/HDL ratio	0.81	−0.04	0.02	0.45	0.00
HOMA-IR	0.75	0.47	0.03	−0.10	0.03
Triglycerides	0.69	0.04	0.16	0.28	−0.01
Average glucose	0.07	0.93	0.17	−0.13	−0.07
HbA_1C	0.07	0.93	0.17	−0.13	−0.07
Fasting glucose	0.17	0.86	0.10	−0.07	−0.10
eGFR	−0.03	−0.14	−0.92	0.02	−0.02
Creatinine	0.23	0.12	0.86	−0.11	0.11
BUN	0.15	0.27	0.77	−0.03	0.01
CCL	0.40	0.06	−0.76	−0.12	0.13
Cholesterol	−0.08	−0.13	−0.01	0.97	0.08
LDL	0.13	−0.17	−0.06	0.92	0.09
Total protein	0.10	0.01	0.11	0.02	0.84
Albumin	−0.07	−0.20	−0.08	0.12	0.76

Note: Bolded values indicate an absolute loading value of 0.40 or above.

HDL: high-density lipoprotein; LDL: low-density lipoprotein; Chol: cholesterol; HbA_1C: glycated hemoglobin; HOMA-IR: homeostatic model assessment of insulin resistance; BUN: blood urea nitrogen; eGFR: estimated glomerulus filtration rate; CCL: creatinine clearance.

An additional unrotated factor matrix was created to determine the potential influence of the rotation on the overall results. This procedure showed that 10 of 16 variables mainly converged into the first factor (Supplementary Table 1), which was less clearly defined then when using Varimax factor rotation. A more conservative set of factors that did not include variables with an age component (eGFR and CCL) was also created (Supplementary Table 2). This analysis resulted in the same five significant factors; though the variance explained by each factor differed slightly and therefore the factor equivalent to primary factor 3 (KFF) was the fourth factor in this secondary analysis (referred to below as secondary factor 4).

Voxel-wise associations between factors and white matter microstructure

Factor 1 (IHF) and Factor 3 (KFF) showed associations that were almost completely segregated regionally and by diffusion metric. Specifically, IHF showed strongest associations with axial diffusivity that were qualitatively determined to be anatomically distributed within long projection fibers, whereas KFF showed strongest associations in radial diffusivity that seemed to be anatomically preferential to the deep periventricular white matter (see Figure 1 and its caption for more details). The overlap in associations was primarily limited to commissural fibers (mainly FA associations in the corpus callosum). While we did not explicitly correct for multiple comparisons across factors, both IHF and KFF were significantly associated with at least two diffusion metrics at p < 0.01 after voxel-wise correction for multiple comparisons. The results are shown at p < 0.05 to evidence the very low overlap in effects between both factors.

Figure 1.

Association between primary factor scores and diffusion measures in all participants.

Secondary voxel-wise analyses using ‘surrogate variables’ (the single variable that had greatest loading on IHF and KFF, respectively) also showed the representative dissociation of their associations with measures of white matter microstructure as shown in Supplemental Figure 1.

Secondary/confirmatory analyses using ROI data

ROIs were extracted from the primary voxel-wise analyses to determine the distribution of the data and to confirm that the results were not substantially influenced by age-adjusted indicators or other aspects of the primary factor analysis. Correlations within ROIs remained significant, although reduced in power, for each of the secondary analyses as demonstrated in the scatterplots presented in Figures 2 and 3. Race and education were not significantly related to the factor scores or the extracted ROI data in our sample (Supplementary Figure 2). Additional analyses splitting participants into groups based on whether any of their factor scores diverged too far from the mean similarly showed that each factor was still associated with at least one diffusion measure in the healthiest (Supplementary Figure 3 and Supplementary Table 3).

Figure 2.

Scatterplots of the associations between insulin/HDL factor (IHF) and diffusion measures.

Figure 3.

Scatterplots of the associations between kidney function factor (KFF) and diffusion measures.

Discussion

The goal of this cross-sectional study was to assess the relationship between regional white matter microstructural integrity and interindividual variation across a diverse collection of standard normal physiological blood markers used clinically to monitor health. Widespread associations with brain tissue microstructure were found for markers of metabolic health and markers of kidney function. Less optimal metabolic health was associated with decreased axial diffusivity in the deep white matter and projection fiber systems, while less optimal kidney function was related to increased mean and radial diffusivity in the periventricular and watershed white matter regions. Associations were statistically independent of age and sex and remained apparent when limiting the sample to only the healthiest individuals, outside of the clinical range of high risk or disease. The differentiation of these effects both on a regional basis and in terms of which diffusion measures are affected suggests distinct mechanisms by which these systemic markers affect the integrity of the brain white matter. These cross-sectional findings may therefore suggest complementary and dissociable influence of subtle variability in systemic physiology on the health of the central nervous system and may further suggest multiple unique routes of therapeutic intervention to explore towards the potential attenuation of neurological aging. Given the potential sensitivity of brain tissue to variations in these health parameters, the findings reported here may have substantial public health implications and may inform neurological practice in reducing risk for age-associated cognitive decline and dementia but may also have implications for general medicine overall. Future interventional studies should focus on optimizing these systemic markers in longitudinal care and determining whether improvement in brain integrity and delay in cognitive decline may be observed.

Previous work by our group^2,12,13,21 and others^1,22–27 have demonstrated cross-sectional associations between variation in health parameters and white matter microstructure. Our work extends these preliminary findings to evaluate associations with blood physiological parameters generally considered in “normal/healthy” ranges. Unexpectedly, we found not only that indices of metabolic health (IHF) and kidney function (KFF) had strong associations with regional microstructural integrity but also that these effects were distinctly unique. The IHF was more highly associated with regional changes in axial diffusivity in the deep white matter and projection fiber systems. Alterations in the KKF in contrast were more highly associated with regional changes in mean and radial diffusivity in the periventricular and watershed white matter regions, which are regions prone to white matter hyperintensities of vascular origin. The major overlapping effects were limited to findings in the commissural fibers (primarily in the corpus callosum). These fibers, particularly the anterior segments, are known to be particularly vulnerable to the aging process,²⁸ and these results may therefore elucidate this vulnerability through sensitivity to multiple mechanisms of deterioration. The significance of the differential sensitivity to diffusion measures is unclear. Axial diffusivity, primarily associated with the IHF, has been linked to axonal injury²⁹; however, like our previous study in an overlapping sample on insulin resistance¹², poorer health was linked to reduced axial diffusivity, contrary to the increase in overall diffusivity observed with typical aging.²⁸ In contrast, radial diffusivity, primarily associated with the KFF, has been linked to demyelination^30,31 and was increased as expected with poorer health. It is, however, clear that multiple processes contribute to variations in diffusion signal, and it is completely unknown from this work whether the associations are driven by variation in any specific histological parameter. Furthermore, it is possible that regions of crossing fibers such as the periventricular regions may be uniquely vulnerable to certain processes, and detection is similarly more sensitive for certain diffusion measures in crossing fibers compared to non-crossing regions. Future work will be necessary to disentangle the regional nature of the associations from the diffusional sensitivity.

This study also adds strong evidence to recent findings of associations between markers of kidney function and brain tissue health in the range of healthy variation.¹⁴ This is in contrast to the effect of significant kidney dysfunction which is known to contribute to neurological complications in ESRD.^32,33 By ESRD white matter alterations are widespread in the brain.^9–11,34,35 Such changes were suspected to be due to severe disease, however, because renal function slowly decreases with aging as a normal biological phenomenon linked to cellular and organ senescence,³⁶ the results here suggest that even subclinically reduced renal function may also contribute to decline in white matter microstructure which is known to be a prominent effect of aging. Previous research suggests that retention of solutes that are toxic in high concentration, such as urea, creatinine, parathyroid hormone, and myoinositol may damage the brain in CKD.³⁷ Alternatively, it is possible that vascular compromise may contribute independently to kidney insufficiency and hypoperfusion and ischemic damage of brain tissue.³⁸ Greater small-vessel disease burden has been shown to be associated with greater renal impairment.^39,40 Furthermore, the fact that the volume of white matter hyperintensity of presumed vascular origin was correlated with serum creatinine in individuals with CKD may support this notion.⁴¹ The finding of dominant distribution of clusters associated with kidney factor in frontal and parietal periventricular white matter, regions also prone to white matter hyperintensities of presumed vascular origin, may also support an independent vascular mechanism to variation in both kidney function and white matter tissue health.

This study also demonstrates that a broader spectrum of overall health contributes to our prior result linking insulin resistance and white matter integrity.¹² Several blood markers were strongly related to insulin resistance and formed the IHF, such as HDL, insulin and triglycerides levels, which in turn were related to the same effects on white matter integrity reported in our previous study solely for insulin resistance. While the hypothesis of our previous study was correct in linking insulin resistance and white matter integrity, the current work demonstrates the importance of a more holistic and data-driven approach revealing that there is a more complex metabolic process that may underlie this association and involve both glucose and fat regulation. Such an approach is needed in future research, for instance to further understand the link between insulin resistance and disorders such as Alzheimer's disease.

There are several limitations to this work that are being addressed in ongoing studies. The results presented here are cross-sectional, and therefore no mechanistic causal inference about directionality can made. The findings do, however, provide suggestive links and follow-up work in longitudinal and interventional cohorts will clarify the role of each of the presented health factors on brain deterioration. While we did not find any effect of race on the results, another limitation is that the sample used in this study consists predominantly of white Caucasian middle-aged and older adults from the Boston metropolitan area and may not allow generalization of the findings to other populations. For instance, we did not find an association between white matter integrity and the blood factor strongly related LDL and total cholesterol, despite finding such an association previously in a completely distinct sample which was disproportionately African American compared to the current sample but also was partially enrolled dependent upon meeting the criteria of having at least one first-degree familial relative with a diagnosis of dementia.¹³ The blood markers available for this work may also not reflect the ideal set and future work will require larger marker sets including metabolomic and proteomic data in large participant samples to better track down the primary systemic factors linked to brain health. Additionally, future research will also aim to investigate how potential redundancy between blood markers may affect the factor structure obtained, and will aim to account for other potential confounders such as hydration status not considered here. Finally, although several studies have demonstrated associations between white matter integrity measured by DTI and cognitive performance, we did not examine cognitive associations here as this topic requires an additional primary focus. Despite these limitations, the results present a novel framework for understanding the aging brain in health and disease and provide valuable information regarding follow-up work to understand potential pathophysiologic mechanisms contributing to neural variation with typical aging.

Footnotes

Funding

The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This study was supported by the NIH grant R01NR010827 using resources provided by NIH grants NS042861, NS058793 and by the Center for Functional Neuroimaging Technologies, P41RR14075, a P41 Regional Resource supported by the Biomedical Technology Program of the National Center for Research Resources (NCRR), NIH. This work also involved the use of instrumentation supported by the NCRR Shared Instrumentation Grant Program and/or High-End Instrumentation Grant Program; specifically, grant numbers S10RR021110, S10RR023401, S10RR019307, S10RR019254 and S10RR023043. JPC was supported by the Fonds Québécois de la Recherche – Santé and by the HST IDEA2 Program supported by the Peter C. Farrell (1967) Fund.

Acknowledgments

The authors would like to thank Paul J. Wilkens for his help with the diffusion data.

Declaration of conflicting interests

The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Authors' contribution

Chang-Woo Ryu: study concept and design, analysis and interpretation of data, statistical analysis, drafting/revising the manuscript for content; Jean-Philippe Coutu: study concept and design, analysis and interpretation of data, statistical analysis, drafting/revising the manuscript for content; Anna Greka: interpretation of data and revising the manuscript for content; H Diana Rosas: acquisition of data, interpretation of data and revising the manuscript for content; Geon-Ho Jahng: interpretation of data and revising the manuscript for content; Bruce R Rosen: interpretation of data and revising the manuscript for content; David H Salat: study concept and design, acquisition of data, interpretation of data and drafting/revising the manuscript for content.

Supplementary material

Supplementary material for this paper can be found at

References

Leritz

Salat

Milberg

et al.

Variation in blood pressure is associated with white matter microstructure but not cognition in African Americans. Neuropsychology 2010; 24: 199–208.

Salat

Williams

Leritz

et al.

Inter-individual variation in blood pressure is associated with regional white matter integrity in generally healthy older adults. Neuroimage 2012; 59: 181–192.

Maillard

Seshadri

Beiser

et al.

Effects of systolic blood pressure on white-matter integrity in young adults in the Framingham Heart Study: a cross-sectional study. Lancet Neurol 2012; 11: 1039–1047.

Stolk

Breteler

Ott

et al.

Insulin and cognitive function in an elderly population. The Rotterdam Study. Diabetes Care 1997; 20: 792–795.

Bruehl

Sweat

Hassenstab

et al.

Cognitive impairment in nondiabetic middle-aged and older adults is associated with insulin resistance. J Clin Exp Neuropsychol 2010; 32: 487–493.

Heringa

van den Berg

Dekker

et al.

Albuminuria and cognitive functioning in an older population: the Hoorn study. Demen Geriatr Cogn Disord 2011; 32: 182–187.

Joosten

Izaks

Slaets

et al.

Association of cognitive function with albuminuria and eGFR in the general population. Clin J Am Soc Nephrol 2011; 6: 1400–1409.

Hoogenboom

Marder

Flores

et al.

Cerebral white matter integrity and resting-state functional connectivity in middle-aged patients with type 2 diabetes. Diabetes 2014; 63: 728–738.

Kong

Wen

et al.

Diffuse interstitial brain edema in patients with end-stage renal disease undergoing hemodialysis: a tract-based spatial statistics study. Medicine 2014; 93: e313.

10.

Chou

Hsieh

Lin

et al.

Widespread white matter alterations in patients with end-stage renal disease: a voxelwise diffusion tensor imaging study. AJNR Am J Neuroradiol 2013; 34: 1945–1951.

11.

Kim

Park

Bai

et al.

Diffusion tensor imaging findings in neurologically asymptomatic patients with end stage renal disease. NeuroRehabilitation 2011; 29: 111–116.

12.

Ryu

Coutu

Rosas

et al.

Effects of insulin resistance on white matter microstructure in middle-aged and older adults. Neurology 2014; 82: 1862–1870.

13.

Williams

Leritz

Shepel

et al.

Interindividual variation in serum cholesterol is associated with regional white matter tissue integrity in older adults. Hum Brain Mapp 2013; 34: 1826–1841.

14.

Sedaghat

Cremers

de Groot

et al.

Kidney function and microstructural integrity of brain white matter. Neurology 2015; 85: 154–161.

15.

Levey

Stevens

Schmid

et al.

A new equation to estimate glomerular filtration rate. Ann Intern Med 2009; 150: 604–612.

16.

Reese

Heid

Weisskoff

et al.

Reduction of eddy-current-induced distortion in diffusion MRI using a twice-refocused spin echo. Magn Reson Med 2003; 49: 177–182.

17.

Smith

Jenkinson

Johansen-Berg

et al.

Tract-based spatial statistics: voxelwise analysis of multi-subject diffusion data. Neuroimage 2006; 31: 1487–1505.

18.

Yendiki

Koldewyn

Kakunoori

et al.

Spurious group differences due to head motion in a diffusion MRI study. Neuroimage 2013; 88C: 79–90.

19.

Smith

Nichols

. Threshold-free cluster enhancement: addressing problems of smoothing, threshold dependence and localisation in cluster inference. Neuroimage 2009; 44: 83–98.

20.

National Cholesterol Education Program Expert Panel on Detection E, Treatment of High Blood Cholesterol in A. Third report of the National Cholesterol Education Program (NCEP) Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults (Adult Treatment Panel III) final report. Circulation 2002; 106: 3143–3421.

21.

Jacobs

Leritz

Williams

et al.

Association between white matter microstructure, executive functions, and processing speed in older adults: the impact of vascular health. Hum Brain Mapp 2013; 34: 77–95.

22.

Aine

Sanfratello

Adair

et al.

Characterization of a normal control group: are they healthy?

Neuroimage 2014; 84: 796–809.

23.

Bender

Raz

. Normal-appearing cerebral white matter in healthy adults: mean change over 2 years and individual differences in change. Neurobiol Aging 2015; 36: 1834–1848.

24.

Bettcher

Walsh

Watson

et al.

Body mass and white matter integrity: the influence of vascular and inflammatory markers. PloS One 2013; 8: e77741.

25.

Bolzenius

Laidlaw

Cabeen

et al.

Impact of body mass index on neuronal fiber bundle lengths among healthy older adults. Brain Imaging Behav 2013; 7: 300–306.

26.

Lin

et al.

Body mass index correlates negatively with white matter integrity in the fornix and corpus callosum: a diffusion tensor imaging study. Hum Brain Mapp 2013; 34: 1044–1052.

27.

Savolainen

. Microstructural white matter changes in metabolic syndrome: a diffusion tensor imaging study. Neurology 2010; 74: 1006–1007. author reply 1007.

28.

Rosas

Doros

Gevorkian

et al.

PRECREST: a phase II prevention and biomarker trial of creatine in at-risk Huntington disease. Neurology 2014; 82: 850–857.

29.

Budde

Xie

Cross

et al.

Axial diffusivity is the primary correlate of axonal injury in the experimental autoimmune encephalomyelitis spinal cord: a quantitative pixelwise analysis. J Neurosci 2009; 29: 2805–2813.

30.

Song

Yoshino

et al.

Demyelination increases radial diffusivity in corpus callosum of mouse brain. NeuroImage 2005; 26: 132–140.

31.

Song

Sun

Ramsbottom

et al.

Dysmyelination revealed through MRI as increased radial (but unchanged axial) diffusion of water. NeuroImage 2002; 17: 1429–1436.

32.

Brouns

De Deyn

. Neurological complications in renal failure: a review. Clin Neurol Neurosurg 2004; 107: 1–16.

33.

De Deyn

Saxena

Abts

et al.

Clinical and pathophysiological aspects of neurological complications in renal failure. Acta Neurol Belg 1992; 92: 191–206.

34.

Hsieh

Chang

Chuang

et al.

End-stage renal disease: in vivo diffusion-tensor imaging of silent white matter damage. Radiology 2009; 252: 518–525.

35.

Zhang

Liu

Yang

et al.

Reduced white matter integrity and cognitive deficits in maintenance hemodialysis ESRD patients: a diffusion-tensor study. Eur Radiol 2015; 25: 661–668.

36.

Glassock

Winearls

. Ageing and the glomerular filtration rate: truths and consequences. Trans Am Clin Climatol Assoc 2009; 120: 419–428.

37.

Fraser

Arieff

. Nervous system complications in uremia. Ann Intern Med 1988; 109: 143–153.

38.

Sedaghat

Vernooij

Loehrer

et al.

Kidney function and cerebral blood flow: the Rotterdam Study. J Am Soc Nephrol 2015; 27: 715–721.

39.

Makin

Cook

Dennis

et al.

Cerebral small vessel disease and renal function: systematic review and meta-analysis. Cerebrovasc Dis 2015; 39: 39–52.

40.

Akoudad

Sedaghat

Hofman

et al.

Kidney function and cerebral small vessel disease in the general population. Int J Stroke 2015; 10: 603–608.

41.

Khatri

Wright

Nickolas

et al.

Chronic kidney disease is associated with white matter hyperintensity volume: the Northern Manhattan Study (NOMAS). Stroke 2007; 38: 3121–3126.

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