Prognostic Value of Immune-Related lncRNA SBF2-AS1 in Diffuse Lower-Grade Glioma

Abstract

LncRNA SET-binding factor 2 (SBF2) antisense RNA1 (SBF2-AS1) has been proven to play an oncogenic role in various types of tumors, but the prognostic role of SBF2-AS1 in tumors, especially in diffuse lower-grade glioma (LGG), is still unclear. Here, we aimed to investigate the prognostic value of SBF2-AS1 in LGG. The LGG expression profiles from The Cancer Genome Atlas (TCGA, n = 524) and Chinese Glioma Genome Atlas (CGGA, n = 431) were mined by Kaplan-Meier analysis, Cox regression analysis, Chi-square test and GSEA analysis. Through Kaplan-Meier analysis, we found the prognosis of LGG patients with high expression of SBF2-AS1 were worse than that of patients with low expression (Log Rank P < 0.001). Cox analysis showed SBF2-AS1 was an independent prognostic factor for poorer overall survival in LGG (P < 0.05). SBF2-AS1 was found to be significantly related to IDH mutation status and SBF2-AS1 was highly expressed in IDH wildtype group. GSEA analysis obtained a total of 126 GO terms and 6 KEGG pathways that were significantly enriched in SBF2-AS1 high expression phenotype (NOM P value < 0.05). We found these 126 GO terms and KEGG pathways were mainly related to immunity. In conclusion, lncRNA SBF2-AS1 expression is an immune-related lncRNA associated with unfavorable overall survival in LGG. SBF2-AS1 could be a reliable prognostic biomarker for patients with LGG.

Keywords

diffuse lower-grade gliomas SBF2-AS1 prognostic biomarker survival immunity

Introduction

Glioma is the most common type of intracranial malignant tumor, and can be grade I to IV for diagnosis according to World Health Organization (WHO) classification. Grades II and III gliomas are defined as diffuse lower-grade glioma (LGG).^1,2 Compared with Grade I and IV, LGGs are more heterogeneous, and accurate prognosis judgment is more difficult, so prognostic markers are more needed for clinical guidance. With the application of molecular characteristics (such as isocitrate dehydrogenase (IDH) mutation status) in the grouping of gliomas,^3,4 it is increasingly recognized that molecular biomarkers play a critical role in the prognosis evaluation of LGG.

Long noncoding RNAs (lncRNAs) are a class of non-protein encoded transcripts longer than 200 bp, which play an important role in normal physiological functions such as growth and development and in various human diseases, especially cancer regulate.^5,6 Emerging evidence suggests that lncRNAs have a critical regulatory role in tumour progression and can predict the prognosis of cancer patients. In the field of glioma research, many lncRNAs have been reported to be closely related to tumorigenesis, development, and prognosis. For example, LINC01116 was found to be involved in tumorigenesis of glioma by targeting VEGFA through miR-31-5p.⁷ LncRNA HERC2P2 was identified as a tumor suppressor, and its overexpression could significantly inhibit the migration and growth of gliomas in vitro and in vivo.⁸ Others have reported that lncRNAs could be prognostic indicators of LGG, such as GAS5, H19, RAB6C-AS1.^9
-11

LncRNA SET-binding factor 2 (SBF2) antisense RNA1 (SBF2-AS1), located on Chromosome 11 p15.4(9,758,268-9,811,335 [GRCh38/hg38]), is widely expressed in various tissues. The tumor-promoting function of SBF2-AS1 was first discovered in non-small cell lung cancer (NSCLC) and was confirmed in various tumors. Meanwhile, SBF2-AS1 was also found to predict the prognosis of cancer patients. For instance, SBF2-AS1 has been reported to promote proliferation of NSCLC cells in vitro or in vivo and regulate the radiosensitivity and apoptosis of NSCLC through microRNA-302a/MBNL3 axis.¹² In cervical cancer, SBF2-AS1 as ceRNA regulates the expression levels of miR-361-5p and FOXM1 to promote the tumour progression.¹³ Moreover, up-regulated SBF2-AS1 can promote cell proliferation in esophageal squamous cell carcinoma.¹⁴ As for hepatocellular carcinoma, SBF2-AS1 can promote tumour metastasis and its high expression indicates poor prognosis.¹⁵ By sponging microRNA-143, SBF2-AS1, serving as ceRNA, can accelerate breast cancer tumorigenesis and progression.¹⁶ However, in LGG, it is not known whether SBF2-AS1 has a oncogenic role and can be used as a prognostic marker.

As the development of next generation sequencing technology, massive high-throughput data and bioinformatics methods have facilitated a comprehensive understanding of tumorigenesis and finding novel prognostic markers or therapeutic drugs.^17
-19 Here, lncRNA expression data from a total of 955 patients with LGG were collected from the Cancer Genome Atlas (TCGA) database and the Chinese Glioma Genome Atlas (CGGA). We aimed at mining the large queue of expression profiles and clinical information to reveal the prognostic indicator efficacy of SBF2-AS1 and to investigate the potential role of SBF2-AS1 in the LGG.

Materials and Methods

Data Collection of Diffuse Lower Grade Glioma (LGG) Patients

The gene expression profile of patients with LGG from The Cancer Genome Atlas Program (TCGA) (Release Date: December 18, 2019; Embargo Release Date: August 17, 2018) was measured experimentally using the Illumina HiSeq 2000 RNA Sequencing platform by the University of North Carolina TCGA genome characterization center, which could be obtained from the UCSC Xena browser (https://xenabrowser.net/).²⁰ Another dataset was downloaded from Chinese Glioma Genome Atlas (CGGA) database (http://www.cgga.org.cn/).^21,22The BIGD accession number of LGG dataset from CGGA is PRJCA001747 (https://bigd.big.ac.cn/bioproject/browse/PRJCA001747). The corresponding clinical data of these LGG patients were also collected. Clinical details of patients were shown in Table 1.

Table 1.

Clinical Information of LGG Patients From TCGA and CGGA Datasets.

Characteristic	TCGA	CGGA
Age
≤ 40	261	221
> 40	263	210
Sex
Female	237	193
Male	287	238
Grade
Unknown	1	0
WHO II	257	180
WHO III	266	251
IDH mutation status
Unknown	399	38
Mutant	91	297
Wildtype	34	96
Radio status
Unknown	67	31
NO	173	86
YES	284	314
Histology
Astrocytoma	194	124
Mixed glioma	133	226
Oligodendroglioma	197	81
1p19q codeletion status
Unknown		38
Codel		128
Non-codel		265

Exploring the Association Between SBF2-AS1 and Clinicopathologic Parameters

X-tile software was used to select optimal cutoff value of the SBF2-AS1 expression value in TCGA and CGGA datasets.^23,24 Then LGG patients were classified into high-expressed or low-expressed group by SBF2-AS1. Survival analysis including Kaplan-Meier, Cox regression analysis was used to explore the prognosis performance of SBF2-AS1. Chi-square test was performed to obtain the relationship between SBF2-AS1 and clinical parameters.

Bioinformatics Analysis of Function Prediction

The above group information of LGG according to the SBF2-AS1 expression was treated as a phenotype label. Then GSEA was performed to analyze the GO and KEGG pathways based on molecular signatures database (c2.cp.kegg.v5.2.symbols.gmt and c5.bp.v5.2.symbols.gmt).²⁵ The GO terms and KEGG pathways were selected by Nominal P value and Normalized enrichment score (NOM P < 0.05).

Results

The Performance of SBF2-AS1 in Predicting the OS of LGG Patients in TCGA and CGGA Datasets

There were 955 LGG patients obtained from the TCGA (n = 524) and CGGA (n = 431) with lncRNAs expression profiles and the corresponding follow-up information for next analysis (Table 1). To investigate the clinical value of SBF2-AS1 in predicting OS of LGG patients, we classified patient with LGG into high- or low-expressed group based on the cutoff value of SBF2-AS1 expression selected by X-tile software, and performed Kaplan–Meier analysis. In TCGA dataset, Kaplan–Meier showed that the survival of high-expressed SBF2-AS1 group (n = 88, median survival time: 2.03 years) was shorter than the low-expressed group (n = 436, median survival time: 7.88 years), and the expression of SBF2-AS1 could distinguish LGG patients with different survival (log-rank P < 0.001; Figure 1A). Then we tested the predictive ability of clinical outcome for SBF2-AS1 in another independent LGG dataset (CGGA, n = 431). The cutoff value of SBF2-AS1 selected by X-tile in CGGA separated LGG patients into 2 groups and Kaplan–Meier analysis verified that the SBF2-AS1 could also assess the prognostic risk of LGG patients (5-year survival: 25.58% vs. 58.43%, log-rank P < 0.001; Figure 1B). Subsequently, in order to describe the relationship between the expression of SBF2-AS1 and survival information, we put them in Figure 2. LGG patients with low expression of SBF2-AS1 lived longer than patients with high expression in the TCGA dataset (Figure 2A) and in the CGGA group (Figure 2B).

Figure 1.

Kaplan–Meier analysis showed LGG patients could be classified into 2 groups with significantly different survival based on SBF2-AS1 expression in the TCGA (A) and CGGA (B) datasets.

Figure 2.

The expression of SBF2-AS1, survival status and survival time for LGG patients in the TCGA (A) and CGGA (B) datasets. X axis represents the index of samples. Red color means that the survival status of patients was dead, and black color means that the survival status of patients was alive.

The Association Between SBF2-AS1 and Clinical Parameters of LGG

Although the expression of SBF2-AS1 was related to Age, Radio status and Grade in TCGA dataset, the CGGA group could not verify the association (P < 0.05, Table 2). However, as shown in Table 2, Chi-square test found the expression of SBF2-AS1 was related to IDH mutation status in 2 datasets (P < 0.001, Table 2). The SBF2-AS1 expression of IDH mutation group was lower than that of IDH wildtype group in TCGA (Figure 3A) and CGGA (Figure 3B) datasets.

Table 2.

Association of SBF2-AS1 Expression With Clinicopathological Characteristics in LGG Patients.

Variables	TCGA		P ^a	CGGA		P ^a
Variables	Low	High	P ^a	Low	High	P ^a
Age			<0.001			0.266
≤ 40	242	19		201	20
> 40	194	69		183	27
Sex			0.181			0.158
Female	191	46		177	16
Male	245	42		207	31
IDH mutation			<0.001			<0.001
Unknown	333	66		35	3
Mutant	83	8		284	13
Wildtype	20	14		65	31
Radio			0.010			0.625
Unknown	50	17		26	5
NO	155	18		77	9
YES	231	53		281	33
1p19q codeletion status						0.001
Unknown				38	0
Codel				121	7
Non-codel				225	40
Grade			0.002			0.724
Unknown	1	0
WHO II	229	28		162	18
WHO III	206	60		222	29

^a The Chi-squared test, P value < 0.05 was considered significant.

Figure 3.

SBF2-AS1 expression patterns in IDH wildtype and mutant groups in the TCGA (A) and CGGA (B) datasets.

SBF2-AS1 Is an Independent Risk Factor for LGG

In TCGA and CGGA dataset, Cox regression analyses were performed. The univariate Cox analysis results indicated Grade, IDH mutation status and the SBF2-AS1 expression were risk factors of LGG; The multivariable Cox analysis obtained that the SBF2-AS1 expression was an independent risk factor of LGG (High vs. Low, HR TCGA = 2.35, 95% CI 1.61–3.45, P < 0.001; HR CGGA = 1.56, 95% CI 1.00–2.41, P < 0.05, Table 3). All above results suggested SBF2-AS1 was a reliable prognostic indicator of LGG.

Table 3.

Cox Regression Analysis of SBF2-AS1 Expression and Survival of LGG Patients in the TCGA and CGGA Datasets.

		Univariable Analysis				Multivariable Analysis
Variables		HR	95% CI of HR		P	HR	95% CI of HR		P
Variables		HR	Lower	Upper	P	HR	Lower	Upper	P
TCGA
Age	>40 vs. ≤40	2.816	1.962	4.041	<0.001	1.707	0.539	5.407	0.364
Gender	Male vs. Female	1.139	0.811	1.599	0.452	1.491	0.542	4.102	0.440
Grade	III vs II,	3.307	2.285	4.787	<0.001	1.398	0.480	4.072	0.540
IDH mutation status	Wildtype vs. Mutant	5.532	2.065	14.820	0.001	2.891	0.909	9.193	0.072
Signature	High risk vs. low risk	4.647	3.226	6.694	<0.001	3.710	1.203	11.436	0.022
CGGA
Age	>40 vs. ≤40	1.188	0.892	1.581	0.239	1.186	0.882	1.596	0.258
Gender	Male vs. Female	1.004	0.753	1.339	0.976	1.140	0.845	1.537	0.392
Grade	III vs II	2.623	1.888	3.645	<0.001	2.970	2.103	4.196	<0.001
IDH mutation status	Wildtype vs. Mutant	2.245	1.642	3.069	<0.001	2.043	1.439	2.902	<0.001
Signature	High risk vs. low risk	2.354	1.606	3.449	<0.001	1.563	0.999	2.445	0.037

Prognostic Value of SBF2-AS1 Co-Expressing Network

We constructed the co-expression network of SBF2-AS1 based on TCGA (n = 524) and CGGA (n = 431) datasets by Pearson test and obtained 202 genes that co-expressed with SBF2-AS1 in both datasets (Pearson coefficient > 0.4/<− 0.4, P < 0.001, Figure 4A). KM survival analysis identified 106 genes which were associated with the prognosis of LGG in the 2 sets of data sets (log Rank P < 0.05). Among them, ANKRD29 was a protective factor (Cox coefficient < 0, P < 0.05) and other 105 genes were risk factors for LGG (Cox coefficient > 0, P < 0.05, Figure 4B). SBF2-AS1 co-expressed with 105 genes related to poor prognosis, suggesting that SBF2-AS1 may interact with these genes and promote tumor progression. To display the correlation of these prognostic genes with survival, KM survival curves of the protective gene (ANKRD29) and one randomly selected risk gene (BANK1) from the above 105 risk genes were showed (Figure 4C-F).

Figure 4.

Prognostic value of SBF2-AS1 co-expressing network (A) Constructing the SBF2-AS1 co-expressing network based on TCGA and CGGA datasets by Pearson test (Pearson coefficient > 0.4/<− 0.4, P < 0.001). Distribution statistics of the 202 genes associated with LGG prognosis by Kaplan–Meier and Cox regression analysis (B). Kaplan–Meier curves of ANKRD29 and BANK1 in TCGA (C and D) and CGGA datasets (E and F).

Function Analysis of SBF2-AS1 by GSEA

Based on the SBF2-AS1 expression phenotype label in 2 datasets, we performed GSEA analysis to explore significantly different pathways between high- and low-expressed SBF2-AS1 groups in TCGA and CGGA datasets, respectively. We obtained 126 GO terms and 6 KEGG pathways that were significantly enriched in both datasets (NOM P value < 0.05, Supplementary Table S2). Then we found the above 126 GO terms and 6 KEGG pathways were mainly clustered in immune-related GO terms and KEGG pathways, such as adaptive immune response, T cell differentiation, B cell activation, immune effector process, lymphocyte mediated immunity, natural killer cell activation, JAK STAT signaling pathway, and Toll like receptor signaling pathway (Figure 5).

Figure 5.

Functional prediction of SBF2-AS1 by GSEA. (A) Immune effector process, (B) Adaptive immune response, (C) B cell activation, (D) T cell differentiation (E) Natural killer cell activation involved ln immune response (F) Lymphocyte mediated immunity, (G) JAK-STAT signaling pathway, (H) Toll like receptor signaling pathway.

Discussion

Patient survival is largely affected by the ability of the tumor to migrate to surrounding normal tissues.²⁶ LGG is highly invasive and heterogeneous, so the prognosis of patients is large and difficult to assess. Over the past decades, the abnormal gene expression (including mRNA, microRNA and lncRNA), gene mutations and epigenetic modifications in LGG have been the research hot-spots of glioma prognostic biomarker. Recently, researchers find that lncRNAs have the advantages of stable existence and can be detected in blood, urine or other body fluids, and have good clinical application value.

Here, after analyzing the expression of SBF2-AS1 in 955 patients with LGG from TCGA and CGGA database, we found that SBF2-AS1 could predict LGG overall survival and be an independent risk factor of overall survival in the 2 large queues of LGG.LGG patients with high expression of SBF2-AS1 were proved to have worse prognosis than patients with low expression. Additionally, the expression of SBF2-AS1 was associated with IDH mutation status, which was regarded as ne of the prognostic indicators of glioma. We also found that patients with IDH mutation had a good prognosis and high expression of SBF2-AS1, and vice versa, confirming the argument that SBF2-AS1 was an indicator of poor prognosis. GSEA analysis found SBF2-AS1 mainly enriched in immune-related GO terms and KEGG pathways, such as adaptive immune response, T cell differentiation, B cell activation, immune effector process, lymphocyte mediated immunity, natural killer cell activation, JAK STAT signaling pathway, and Toll like receptor signaling pathway, suggesting that SBF2-AS1 could participate in tumor progression by regulating immunity.

LncRNA SBF2-AS1 has been reported to serve as an oncogene in a variety of cancers such as lung cancer,^27,28 gastric cancer,²⁹ colorectal cancer³⁰ hepatocellular carcinoma,¹⁵ breast cancer,¹⁶ acute myeloid leukemia,³¹ and clear cell renal cell carcinoma.³² It has been reported that SBF2-AS1 is highly expressed in the tissues of above tumors, and downregulated SBF2-AS1 could inhibit tumor cells proliferation and promote apoptosis. Moreover, SBF2-AS1 has been demonstrated to be associated with poor prognosis in non-small cell lung cancer³³ and hepatocellular carcinoma,¹⁵ indicating its prognostic value in cancer. In glioblastoma, Hai Yu et al found SBF2-AS1 was highly expressed and played an important role in the GBM angiogenesis through NFAT5/SBF2-AS1/miR-338-3p/EGFL7 pathway.³⁴ Zhuoran Zhang et al discovered that SBF2-AS1 was mainly expressed in the cytoplasm of GBM cells and could secrete into serum by exosomes, and identified another function of the oncogenic SBF2-AS1, mediating TMZ resistance.³⁵ Fangkun Luan et al found that SBF2-AS1 was an independent unfavorable prognostic factor in glioma.³⁶ However, there are few reports on the specific function of SBF2-AS1 in LGG patients and its prognostic significance. This article found that SBF2-AS1 had a prognostic value in LGG, which was consistent with the reported results of SBF2-AS1 in other cancers. Furthermore, we found that SBF2-AS1 was related to immunity in functional analysis, suggesting that it may be a target for LGG immunotherapy. In the later research, we need explore the the carcinogenic mechanism of lncRNA SBF2-AS1 in LGG through more biological experiments such as cell proliferation assay, colony formation assay, transwell cell migration and invasion assay. From the perspective of bioinformatics analysis, we plan to explore the specific mechanism of SBF2-AS1 in LGG through constructing a network composed of lncRNA, genes and miRNA.³⁷

Overall, we identified that lncRNA SBF2-AS1 could be a prognostic risk indicator of LGG and the SBF2-AS1 expression was associated with unfavorable survival. SBF2-AS1 has potential to become a good prognostic biomarker.

Supplemental Material

Supplemental Material, sj-xls-1-tct-10.1177_15330338211011966 - Prognostic Value of Immune-Related lncRNA SBF2-AS1 in Diffuse Lower-Grade Glioma

Supplemental Material, sj-xls-1-tct-10.1177_15330338211011966 for Prognostic Value of Immune-Related lncRNA SBF2-AS1 in Diffuse Lower-Grade Glioma by Qiang Zhang, Xiao-Jun Liu, Yang Li, Xiao-Wei Ying and Lu Chen in Technology in Cancer Research & Treatment

Supplemental Material

Supplemental Material, sj-xls-2-tct-10.1177_15330338211011966 - Prognostic Value of Immune-Related lncRNA SBF2-AS1 in Diffuse Lower-Grade Glioma

Supplemental Material, sj-xls-2-tct-10.1177_15330338211011966 for Prognostic Value of Immune-Related lncRNA SBF2-AS1 in Diffuse Lower-Grade Glioma by Qiang Zhang, Xiao-Jun Liu, Yang Li, Xiao-Wei Ying and Lu Chen in Technology in Cancer Research & Treatment

Footnotes

Authors’ Note

Publicly available datasets can be found here,

TCGA: https://xenabrowser.net/datapages/?cohort=TCGA%20Lower%20Grade%20Glioma%20(LGG)&removeHub=https%3A%2F%2Fxena.treehouse.gi.ucsc.edu%3A443.

CGGA: http://www.cgga.org.cn/download?file=download/20200506/CGGA.mRNAseq_693.RSEM-genes.20200506.txt.zip&type=mRNAseq_693&time=20200506

Author Contributions

Qiang Zhang, Xiao-Jun Liu, Yang Li: data collection, data analysis, interpretation and drafting; Xiao-Wei Ying, Lu Chen: study design, study supervision and final approval of the manuscript.

Declaration of Conflicting Interests

The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Funding

The author(s) received no financial support for the research, authorship, and/or publication of this article.

ORCID iD

Xiao-Wei Ying

Supplemental Material

Supplemental material for this article is available online.

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Supplementary Material

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