Platelet Glycoprotein Ia 807c/T and 873g/A Polymorphisms in Patients With Venous Thromboembolism

Abstract

Two silent polymorphisms (807C/T and 873G/A) within glycoprotein Ia (GPIa) gene have been implicated in increased risk of developing thrombosis and myocardial infarction in affected individuals. The aim of this study was to investigate the GPIa gene polymorphism in patients with venous thromboembolism (VTE). A multiplexed allele specific-polymerase chain reaction (AS-PCR)–based method was used to determine the GPIa 807T/873A allele frequency in 77 patients with VTE and 106 healthy controls. The allelic frequency for 807T/873A was 33% in the patient group and 38% in the control group. The allelic frequency for 807C/873G was 66% in the patient group and 62% in the control group. The genotypic frequencies were 8% for 807TT/873AA, 42% for 807CC/GG, and 50% for 807CT/GA in the patient group. In the control group, the frequencies were 12% for 807TT/873AA, 35% for 807CC/873GG, and 52% for 807CT/873GA. As a result, the glycoprotein Ia 807C/T and 873G/A dimorphisms were not shown as risk factors for VTE.

Keywords

Glycoprotein pulmonary embolism deep venous thrombosis

References

Kunicki TJ , Kritzik M , Annis DS , Nugent DJ. Hereditary variation in platelet integrin α2β1 density is associated with two silent polymorphisms in the α2 gene coding sequence. Blood 1997;89: 1939-1939.

Kritzik M , Savage B , Nugent DJ , Santoso S , Ruggeri ZM , Kunicki TJ. Nucleotide polymorphisms in the α2 gene define multiple alleles that are associated with differences in platelet α2β1 density. Blood 1998;92: 2382-2382.

Di Paola J , Federici AB , Mannucci PM , et al. Low platelet α2β1 levels in type I von Willebrand disease correlate with impaired platelet function in a high shear system. Blood 1999;93: 3578-3578.

Santoso S , Kunicki TJ , Kroll H , Haberbosch W , Gardemaan A. Association of the platelet glycoprotein Ia C807T gene polymorphism with nonfatal myocardial infarction in younger patients. Blood 1999;93: 2449-2449.

Moshfegh K , Wuillemin WA , Redondo M , Lammie B , Beer JH , Liechti-Gallati S. Association of two silent polymorphisms of platelet glycoprotein Ia/IIa receptor with risk of myocardial infarction: A case-control study. Lancet 1999;353: 351-351.

Carlsson LE , Santoso S , Spitzer C , Kessler C , Greinacher A. The α2 gene coding sequence T807 A873 of the platelet collagen receptor integrin α2β1 might be a genetic risk factor for the development of stroke in younger patients. Blood 1999;93: 3583-3583.

Dinauer DM , Friedman KD , Hessner MJ. Allelic distribution of the glycoprotein Ia (α2-integrin) C807T/G873A dimorphisms among Caucasian venous thrombosis patients and six racial groups. Br J Haematol 1999;107: 563-563.

Coller BS , Beer JH , Scudder LE , Steinberg MH. Collagenplatelet interaction: Evidence for a direct interaction of collagen with platelet GPIa/IIa and indirect interaction with GPIIb/IIIa mediated by adhesive proteins. Blood 1989;74: 182-182.

Kirchhofer D , Tschoop TB , Steiner B , Baumgartner HR. Role of collagen-adherent platelets in mediating fibrin formation in flowing whole blood. Blood 1995;86: 3815-3815.

10.

Savage B , Marzec UM , Chao BH , Harker LA , Maraganore JM , Ruggeri ZM. Binding of the snake venom-derived proteins applaggin and echistatin to the arginine-glycineaspartic acid recognition site(s) on platelet glycoprotein IIb/III complex inhibits receptor function. J Biol Chem 1990;265: 11766-11766.

11.

Hessner MJ , Dinauer M , Luhm AR , Endres JL , Montgomery RR , Friedman KD. Contribution of the glycoprotein Ia 807TT, methylene tetrahydrofolate reductase 677TT and prothrombin 20210GA genotypes to prothrombotic risk among factor V 1691GA (Leiden) carriers. Br J Haematol 1999;106: 237-237.