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Abstract

Equine ingesta-associated choledocholithiasis is a rare cause of morbidity and mortality. We describe here the clinical, gross, histologic, and microbiologic features of this condition in 2 horses and compare the features to 2 previous cases. Case 1 was a 4-y-old Thoroughbred mare with colic. Case 2 was an 18-y-old American Paint Horse mare with colic, chronic weight loss, and inappropriate mentation. Both had elevated biochemical markers of hepatocellular injury and cholestasis and were euthanized given a poor prognosis. Case 1 had a well-formed 5-cm choledocholith surrounding a piece of hay, and had chronic neutrophilic cholangiohepatitis, bridging fibrosis, and extrahepatic obstruction. Case 2 had an ill-formed choledocholith with occasional hay fragments, wood stick, and twigs, and had regionally extensive hepatocellular necrosis with mild neutrophilic cholangiohepatitis and bridging fibrosis. Enterococcus casseliflavus and Escherichia coli were isolated in both cases; Clostridium spp. were also isolated from case 2. All 4 reported cases had increased activity of cholestatic enzymes, hyperbilirubinemia, portal inflammation, and bridging fibrosis. Colic, pyrexia, leukocytosis with neutrophilia, and elevated hepatocellular enzyme activity were documented in 3 cases. Foreign material in all 4 cases was plant origin (choledochophytolithiasis), including hay (n = 2), sticks/twigs (n = 2), and grass awns (n = 1). Ingesta-associated choledocholithiasis may be considered as a cause of colic, pyrexia, and elevated cholestatic biomarkers in horses.

Keywords

cholangiohepatitis cholangitis cholelithiasis colic foreign body horses liver plant

Formation of biliary tract stones (cholelithiasis), including gallbladder stones (choleliths) and common bile duct stones (choledocholiths), are relatively uncommon phenomena in animals.^16,17,20,29 Specifically, horses, which lack a gallbladder, are reported to develop choledocholiths with an estimated prevalence of 0.08%.²¹ Equine choledocholithiasis can be clinically silent and only detected at autopsy; alternatively, choledocholiths can lead to cholangitis, extrahepatic biliary obstruction, hepatic fibrosis, and cholangiohepatitis, ultimately resulting in significant hepatobiliary injury and clinical signs and biochemical indices of hepatobiliary dysfunction.^15,19,28 The pathogenesis of equine choledocholithiasis is unknown, but bacteria, mostly enteric species such as Escherichia coli, Salmonella spp., Enterococcus spp., and others, have been isolated from stones.^10,15 One hypothesis is that bacterial infection ascending from the intestinal tract predisposes to cholelith formation.^2,4 In support of this hypothesis, we report here 2 cases of equine choledocholithiasis associated with ascent of ingested plant material and associated enteric bacteria into the common bile duct.

Case 1 was a 4-y-old Thoroughbred mare that was presented to the North Carolina State University College of Veterinary Medicine (NCSU-CVM; Raleigh, NC, USA) with a 3-d history of intermittent and progressive colic. Rectal palpation by the referring veterinarian (rDVM) identified a suspected large colon displacement. Clinical signs initially resolved with administration of flunixin meglumine, but recurred 2 d later. Pain was refractory to medical intervention, peripheral blood lactate was 5.7 mmol/L (RI: < 2 mmol/L), and referral to the NCSU-CVM was elected. Upon arrival to NCSU-CVM, physical examination revealed mildly decreased borborygmi in the left quadrants and right dorsal displacement of the large colon on abdominal palpation per rectum, which resolved on repeat examination 8 h later. Thoracic and abdominal ultrasound were unremarkable. A CBC revealed moderate neutrophilic leukocytosis and hypofibrinogenemia (Table 1). An initial limited after-hours serum biochemistry panel at NCSU-CVM revealed markedly increased activity in aspartate aminotransferase (AST; 11.4 µkat/L, RI: 2.9–5.7 µkat/L) and gamma-glutamyl transferase (GGT; 8.58 µkat/L, RI: 0.08–0.40 µkat/L), and moderate hyperbilirubinemia (103 µmol/L; RI: 9–39 µmol/L). A repeat serum biochemistry panel specifically evaluating hepatic enzymes and biomarkers was obtained 8 h after initial presentation to the NCSU-CVM. This panel confirmed the initial aberrations in AST, GGT, and bilirubin, and further identified markedly elevated bile acids and moderately increased activity of succinate dehydrogenase (SDH; Table 1). Serum ammonia and lactate were within RIs. Given these findings, severe hepatopathy was suspected. Clinical signs persisted despite medical management. The mare was ultimately euthanized and submitted for postmortem examination.

Table 1.

Relevant CBC and serum biochemistry findings for horses with ingesta-associated choledocholithiasis. Values (RIs) are those seen on presentation at the referral hospitals.

Parameter	Case 1, TB mare	Case 2, APH mare
Leukocytes, ×10⁹/L	19.7 (5.5–12.5)	ND
Neutrophils, ×10⁹/L	17.1 (2.45–6.82)	ND
Fibrinogen, g/L	0.4 (1–4)	ND
ALP, µkat/L	ND	6.56 (1.34–3.12)
GGT, µkat/L	8.10 (0.07–0.32)	2.24 (0.12–0.33)
Total bilirubin, µmol/L	115 (7–43)	170 (9–31)
SDH, µkat/L	0.78 (0.11–0.23)	0.54 (0.07–0.22)
Bile acids, µmol/L	139 (0–15)	50 (4–12)
Triglycerides, mmol/L	ND	1.64 (0.11–0.68)
Lactate, mmol/L	2.0 (0–2.0)	ND

ALP = alkaline phosphatase; APH = American Paint Horse; GGT = gamma-glutamyl transferase; ND = not determined; SDH = sorbitol dehydrogenase; TB = Thoroughbred.

On gross postmortem examination, the liver was firm, resistant to crushing on palpation, and mildly enlarged. At the hilus of the liver, filling and emerging from the common bile duct and extending into the intrahepatic biliary tree, was a firm-to-hard, orange-brown, 3.5 × 3.5 × 5.5-cm ovoid choledocholith with a granular surface (Fig. 1). A few (< 10) similar, 2–5-mm, more friable choleliths were present in the adjacent intrahepatic biliary tree. Regional hepatic lymph nodes were moderately enlarged, measuring up to 4.5 × 2.5 × 0.9 cm (Fig. 1). On cut surface of the liver, bile ducts were prominent with fibrous tissue surrounding portal areas; these changes were most pronounced in parenchyma at the hilus adjacent to the choledocholith. The duct mucosa associated with the choledocholith was red-to-purple and eroded (Fig. 1).

Figures 1–4.

Gross, cytologic, and histologic features of ingesta-associated choledocholithiasis in case 1, a Thoroughbred mare. Figure 1. The opened common bile duct contains a firm-to-hard, orange-brown, 3.5 × 3.5 × 5.5-cm choledocholith with smaller firm ovoid choleliths extending into the intrahepatic duct. The duct mucosa is red-to-purple and eroded. Prominent lymph nodes are adjacent to the duct (arrowheads). Figure 2. The large choledocholith surrounds a central 7 × 0.3 × 0.3-cm piece of hay. Bar = 1 cm. Figure 3. Impression smear of fluid surrounding the choledocholith; degenerate neutrophils admixed with columnar biliary epithelial cells. A few intracytoplasmic (white arrowheads) and extracellular (black arrowheads) bacilli are present. Diff-Quik stain. Figure 4. In portal regions, moderate numbers of inflammatory cells, including neutrophils and fewer lymphocytes, plasma cells, and macrophages, surround and infiltrate the biliary epithelium (inset), extend beyond the limiting plate, and are present within the hepatic parenchyma. There is marked periductular proliferation of myofibroblasts admixed with extensive fibrosis and edema, causing intrahepatic obstruction. H&E.

An ~7 × 0.3 × 0.3-cm linear piece of plant material (hay) was identified at the core of the choledocholith (Fig. 2). The choledocholith was surrounded by 5–10 mL of turbid, yellow-green, opaque fluid that extended into the immediately adjacent intrahepatic bile ducts. Cytologic evaluation of an impression smear of the fluid revealed sloughed columnar biliary epithelial cells admixed with degenerate neutrophils, with scattered extracellular and intracytoplasmic short rods (Fig. 3). Bacterial culture of bile duct fluid yielded heavy growth of E. coli and E. casseliflavus as identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF; VITEK MS, bioMérieux). Both organisms were confirmed with 99.9% confidence, and the identification of E. coli was consistent with the cytologic appearance of bacteria seen on the bile impression smear.

Tissues were collected in 10% neutral-buffered formalin and processed routinely for histologic evaluation with H&E staining. Evaluation of the common bile duct where the choledocholith was lodged revealed moderate mucosal hyperplasia with expansion of the lamina propria by neutrophils, lymphocytes, plasma cells, and macrophages. Inflammation extended into deeper fibrous connective tissue separating the common bile duct from the adjacent pancreas but did not extend into the pancreatic parenchyma, which was histologically within normal limits. Evaluation of the liver revealed moderate-to-marked expansion of portal tracts by neutrophils, with fewer lymphocytes, plasma cells, and macrophages. Inflammatory cells tightly cuffed and infiltrated the biliary epithelium, with multifocal neutrophilic exocytosis, luminal accumulation of degenerate-to-intact neutrophils and necrotic cellular debris, and coagulative necrosis of epithelium, consistent with neutrophilic cholangitis (Fig. 4). In addition, within the portal regions, there was fibrosis, edema, proliferation of a few small caliber bile ducts (ductular reaction), and robust periductular proliferation of concentric spindloid cells (myofibroblasts), consistent with extrahepatic obstruction (Fig. 4). Fibrosis often bridged between portal tracts, and inflammatory cells multifocally extended beyond the limiting plate, with rare individual hepatocellular necrosis in portal areas, consistent with cholangiohepatitis (Fig. 4, inset). Low numbers of canalicular bile plugs were present adjacent to portal tracts.

Collectively, these findings informed a diagnosis in case 1 of segmental choledocholithiasis with plant material with secondary moderate-to-severe, chronic, neutrophilic choledochitis and cholangiohepatitis with bridging fibrosis, extrahepatic obstruction, and ductular reaction. In addition to the hepatic and biliary changes, the associated regional lymph nodes had marked, follicular hyperplasia with formation of prominent germinal centers. The medullary sinuses were moderately expanded by a mixture of macrophages, neutrophils, lymphocytes, and plasma cells. The brain was not evaluated histologically.

Case 2 was an 18-y-old American Paint Horse mare that was referred to The Ohio State University Veterinary Medical Center (OSU-VMC; Columbus, OH, USA) because of colic, chronic weight loss, inappetence, and acute inappropriate mentation. Blood work from the rDVM identified increases in activity of AST, GGT, and alkaline phosphatase (ALP), and hyperbilirubinemia (Suppl. Table 1). The mare was treated initially with intravenous fluids and flunixin meglumine by the rDVM, and referral to OSU-VMC was elected after persistence of clinical signs despite medical management.

On physical examination at the OSU-VMC, the mare was icteric, moderately tachycardic (70 bpm) with a 4 of 6 left holosystolic murmur and bilaterally decreased (ventral) to absent (dorsal) borborygmi. The mare was thin (body condition score: 2.5 of 9) with marked symmetric atrophy of epaxial and gluteal muscles and generalized weakness. Abdominal ultrasound revealed copious amounts of free heteroechoic and flocculated abdominal fluid suggestive of fibrin exudation. The liver was heterogeneous with large hypoechoic foci scattered randomly throughout the parenchyma. Abdominocentesis yielded dark-brown-to-orange turbid fluid with total protein of 38 g/L (RI: < 20 g/L), lactate of 1,750 mmol/L (RI: < 222 mmol/L), and total leukocyte count of 5.34 × 10⁹ cells/L (RI: < 5 × 10⁹ cells/L), with 20% neutrophils and 72% large mononuclear cells. Cytologic evaluation of the abdominal fluid revealed marked neutrophilic and histiocytic inflammation with a mixed population of extracellular bacteria including diplococci, short rods, and elongate rods, collectively suggesting a mixed septic peritonitis. Serum biochemistry performed at the OSU-VMC revealed numerous abnormalities (Table 1; Suppl. Table 2), the most significant of which were moderate-to-marked increases in activity of AST, ALP, GGT, and SDH, hyperbilirubinemia, and increased bile acids. Given the poor prognosis and concern for severe hepatopathy, the mare was euthanized and submitted immediately for postmortem examination.

On gross postmortem examination, there was severe diffuse icterus of the mucous membranes, sclerae, and adipose tissue. The peritoneal cavity contained ~15 L of green-brown-to-red, opaque fluid with no observable organ rupture. Copious amounts of fibrin coated the omentum, spleen, liver, and ventral abdominal body wall; fibrin was most abundant overlying the left side of the liver. Affecting ~50% of the liver, and predominantly the left lobes, were 5–8, multifocal-to-coalescing, large (up to 30 cm diameter), well-demarcated, circular-to-irregularly shaped soft red-brown-to-tan foci of coagulative necrosis. Necrotic areas were emphysematous and, on cut section, were diffusely red-brown. Along the periphery of the left lobe, affecting ~10% of the liver, was a poorly demarcated green region of liquefactive necrosis.

Filling the entire common bile duct, from liver to duodenum, was a 4 × 4 × 12-cm green-yellow, granular concretion (choledocholith; Fig. 5). At the level of the major duodenal papilla, a brown-black 5 × 0.2 × 0.2-cm wood stick extended from the choledocholith toward the duodenum (Fig. 5). After fixation and sectioning of the bile duct, the choledocholith was comprised of bile concretions and pieces of plant material (hay and wood twigs; Fig. 6). Aerobic and anaerobic bacterial culture of the liver yielded heavy growth of Clostridium spp., E. coli, and E. casseliflavus determined via MALDI-TOF MS. The Bruker MALDI Biotyper software interpretative criterion is based on a 1–3 score system.^22–24 Scores of ≥ 2.0 are accepted for identification down to the species level; scores of ≥ 1.7 but < 2.0 are accepted for identification only to the genus level. Scores below 1.7 are considered unreliable. Except for the Clostridium spp., the other pathogens had a score of ≥ 2.2. The Biotyper identified Clostridium haemolyticum with a score of 1.8, and the colony morphology resembled this pathogen. Additional testing was not performed on this isolate.

Figures 5–9.

Gross and histologic features of ingesta-associated choledocholithiasis in case 2, an American Paint Horse mare. Figure 5. Liver (L) with common bile duct opened to the major duodenal papilla and duodenum. The duct (arrowheads) is obstructed by a 4 × 4 × 12-cm, irregularly shaped, green-yellow, friable choledocholith near the major duodenal papilla (white circle), where a 5 × 0.2 × 0.2-cm brown-black wood stick (white arrow; black arrow in Fig. 6) extends from the choledocholith. The duct mucosa is red-to-dark-red with ulceration. Figure 6. The choledocholith was markedly friable and separated artifactually during formalin fixation. The section of wood stick (arrow) is present within the choledocholith concretions. Additional smaller sections of wood (twigs) and plant material (hay) are at the bottom of the frame. Figure 7. Extensive coagulative-to-liquefactive necrosis of the hepatic parenchyma (left side of image). At the interface of necrotic and intact hepatic parenchyma, there is infiltration by large numbers of intact and degenerate neutrophils, abundant fibrin, and myriad bacteria, mostly long rods with fewer short rods and cocci (asterisk). Inset: interface with numerous long rod-shaped bacteria (top left) and degenerate and intact neutrophils. The remaining hepatic parenchyma is congested, and there is thrombosis of a central vein near the region of necrosis (arrowhead). H&E. Figures 8, 9. Gram staining of liver revealed gram-positive endosporulating long rods, consistent with Clostridium spp. (Fig. 8), as well as large gram-positive cocci (Fig. 9, arrowhead), and small gram-negative short rods (Fig. 9, arrow), consistent with Enterococcus casseliflavus and Escherichia coli, respectively. Gram stain.

Tissue samples were collected in 10% neutral-buffered formalin and processed routinely for histologic evaluation with H&E staining. The common bile duct had severe mucosal ulceration with accumulation of abundant intact and degenerate neutrophils, fibrin, and luminal lakes of bile and plant material. Well-demarcated regions of acute coagulative-to-liquefactive necrosis of the hepatic parenchyma affected over half of the liver section randomly (Fig. 7). At the interface of necrotic foci and intact hepatic parenchyma, there was infiltration by large numbers of neutrophils, abundant fibrin, and myriad mixed bacteria, including mostly long rods with fewer short rods and cocci (Fig. 7, inset). Occasionally, the short rods and cocci were present centrally within the necrotic regions. Gram staining revealed 3 populations of bacteria: numerous long gram-positive rods, fewer large gram-positive cocci, and small gram-negative short rods, consistent with Clostridium spp., E. casseliflavus, and E. coli isolated via culture, respectively (Figs. 8, 9). There were rare fibrin thrombi within vasculature at the margin of necrosis (Fig. 7). Distant from regions of necrosis, hepatic portal areas were mildly expanded by a mixture of neutrophils, lymphocytes, and plasma cells that occasionally infiltrated bile ducts, with associated vacuolation and/or necrosis of biliary epithelium, consistent with mild mixed cholangitis. Occasionally, the inflammatory cells extended past the limiting plate into the hepatic parenchyma, consistent with cholangiohepatitis. Within affected portal tracts, there was marked proliferation of small caliber bile ducts (ductular reaction) as well as moderate portal–portal bridging fibrosis. Notably, there was no overt evidence of extrahepatic obstruction (i.e., no periportal myofibroblast proliferation or portal edema).

Collectively, these findings informed diagnoses in case 2 of focally extensive, severe, subacute ulcerative and fibrinosuppurative choledochitis with a grossly identified choledocholith with intra-choledocholith plant material; severe, multifocal-to-coalescing, acute hepatocellular necrosis with intralesional mixed bacteria; and mild, chronic, multifocal neutrophilic cholangitis and cholangiohepatitis with bridging fibrosis and ductular reaction.

Our 2 cases demonstrate a unique cause of equine colic in the form of choledocholithiasis secondary to ingested plant material that had ascended into the common bile duct. We identified 2 additional cases of ingesta-associated choledocholithiasis by searching for “horse cholelithiasis”, “equine cholelithiasis”, and “horse cholelithiasis foreign body” in PubMed, NCSU, and OSU Library databases; one of the cases had a 7-cm wooden stick, and the other case had grass awns, within the core of a choledocholith^7,8 (Table 2). Meaningful evaluation of trends in this cohort is precluded given the low number of cases; however, a few observations can be made.

Table 2.

Features of reported cases of foreign body–associated equine choledocholithiasis.

	Case 1	Case 2	Reference 7	Reference 8
Signalment	4-y-old TB mare	18-y-old APH mare	12-y-old QH gelding	9-y-old TB gelding
Clinical signs	Colic	Colic, chronic weight loss, inappetence, inappropriate mentation; pyrexia	Recurrent colic; pyrexia	Poor race performance, intermittent pyrexia, head pressing, icterus, anorexia, weight loss
Abdominal ultrasound	Unremarkable	Free heteroechoic and flocculated abdominal fluid (fibrin); liver: heterogeneous with large randomly scattered hypoechoic foci throughout parenchyma	Several tortuous tubular anechoic structures in liver (dilated bile ducts) with highly echogenic structures (choleliths)	Hepatomegaly with increased hepatic parenchyma echogenicity and biliary duct distention
CBC	Leukocytosis with mature neutrophilia	NP	Leukocytosis with left shift	Leukocytosis with neutrophilia and lymphopenia
Serum chemistry	↑ AST, GGT, SDH, bile acids; hyperbilirubinemia, hypofibrinogenemia	↑ AST, ALP, GGT, SDH, bile acids, ↑ triglycerides, creatine kinase; hyperbilirubinemia, azotemia, hypoalbuminemia, hypoglobulinemia, hypoglycemia, hypochloremia	↑ SDH, GGT, bile acids; hyperbilirubinemia, hyperfibrinogenemia, decreased BUN	↑ GGT, ALP; hyperbilirubinemia
Abdominocentesis	NP	↑ total protein, lactate; leukocytosis with neutrophils & large mononuclear cells	↑ total protein	NR
Gross findings	Hepatomegaly—firmMultiple cholelithsTurbid bileBiliary ductal fibrosis	Fibrinosuppurative peritonitis; severe fibrinosuppurative cholangiohepatitis with coagulative necrosis, emphysema, and hepatic atrophy	Hepatomegaly—firm, irregular, green-brownDilated extrahepatic bile ducts	Hepatomegaly—firm
Bacterial culture results	Heavy growth of E. coli, E. casseliflavus	Heavy growth of E. coli, E. casseliflavus, Clostridium spp. (suspect C. haemolyticum)	NR	NR
Histologic findings in liver	Suppurative cholangiohepatitis; portal fibrosisCommon bile duct: mucosal hyperplasia; chronic-active choledochitis	Severe acute hepatocellular necrosis with intralesional mixed bacteria; neutrophilic cholangitis and cholangiohepatitis with bridging fibrosis and ductular reaction; common bile duct: subacute ulcerative and fibrinosuppurative choledochitis	Suppurative cholangiohepatitis; bridging fibrosis	Severe bridging portal fibrosis; biliary hyperplasia; microgranulomas with intralesional bile; lymphohistiocytic portal hepatitis
Cholelithiasis-associated foreign material	Hay	Hay and small sticks/twigs	7-cm stick	Lanceolate grass seeds with bristle-like appendages (grass awns) (Avena fatua)

ALP = alkaline phosphatase; APH = American Paint Horse; AST = aspartate aminotransferase; BUN = blood urea nitrogen; GGT = gamma-glutamyl transferase; NP = not performed; NR = not reported; QH = Quarter Horse; SDH = sorbitol dehydrogenase; TB = Thoroughbred.

Ingesta-associated choledocholiths in horses have been reported in a wide age range (4–18 y); 2 of 4 horses were Thoroughbreds.^7,8 Clinically, these horses had signs similar to horses with non–ingesta-associated choledocholiths,¹⁵ including colic (3 of 4), pyrexia (3 of 4), and/or neurologic signs (2 of 4; Table 2).^2,7,8 Only 3 of the 4 horses had CBC data, and for all 3, leukocytosis with neutrophilia was present. Hepatobiliary serum biochemical derangements were common, with elevations in hepatocellular enzyme activity including AST (2 of 4) and SDH (3 of 4), and increased cholestatic enzyme activity (GGT and ALP [in 2 of 4]). All 4 horses had hyperbilirubinemia, and 3 cases had elevated bile acid concentrations.

Three horses, including our case 1, had gross hepatomegaly with firm consistency along with either dilation or fibrosis of the bile ducts.^7,8 Histologically, these cases had lesions consistent with extrahepatic obstruction including myofibroblast proliferation within portal regions, bridging fibrosis, and portal hepatitis and/or neutrophilic cholangiohepatitis.

In contrast, our case 2 had no evidence of extrahepatic obstruction, but instead had large regions of grossly apparent and histologically confirmed hepatic coagulative and liquefactive necrosis with a much less prominent component of cholangiohepatitis and bridging fibrosis. Of note, this is also the only horse of the 4 that had evidence of massive hepatocellular dysfunction (i.e., hypoalbuminemia, hypoglycemia). These distinguishing features from the other 3 reported cases are attributed to the presence of Clostridium spp. hepatitis, with histologic visualization of numerous Clostridium organisms at the front of hepatocellular necrosis as well as confirmation of Clostridium spp. growth on anaerobic bacterial culture of the liver. This pattern is suggestive of infection with either C. novyi (infectious necrotic hepatitis) or C. haemolyticum (bacillary hemoglobinuria).¹⁴ Germination and proliferation of these bacteria is dependent upon exposure to an anaerobic environment¹⁴; in this case, we suspect that choledocholithiasis preceded hepatocellular necrosis.

There are a few potential pathogeneses for the hepatocellular injury in case 2. First, biliary rupture secondary to ascending bacterial cholangitis may have resulted in release of bile into the parenchyma, resulting in regional hepatocellular necrosis (so-called “bile infarcts”) and creation of an anerobic environment. We consider this the most likely pathogenesis. Second, significant extrahepatic obstruction and increased retrograde biliary pressure could similarly result in bile infarcts, but is considered less likely given the lack of histologic evidence of extrahepatic obstruction. Third, intrahepatic bile canalicular cholestasis could have impacted sinusoidal blood flow, leading to hypoxic conditions and hepatocellular necrosis. However, this is also considered less likely given the lack of significant canalicular cholestasis in case 2. In all of these scenarios, local necrosis and an anaerobic environment within the liver could have allowed the Clostridium spp. to proliferate and cause the observed regional hepatic necrosis. Case 2 highlights the potential role of choledocholiths in the pathogenesis of clostridial hepatitis in horses.

Additionally, in the bile or liver of our 2 cases, there was growth of E. coli and E. casseliflavus. Both microbes are common enteric bacteria in horses¹³ and likely reflect ascending infections associated with reflux of enteric contents, including ingesta and intestinal microbiota, into the bile duct leading to colonization of the biliary tree and/or hepatic parenchyma.^7,15 In both ingesta- and non–ingesta-associated choleliths reported in the veterinary and human literature, polymicrobial infections with coliform bacteria are observed frequently, leading to cholangiohepatitis of variable severity.^9,10,21 E. coli has been reported in other cases of equine choledocholithiasis,¹⁵ but E. casseliflavus has not. Although conclusions are limited by the small number of cases, the presence of E. casseliflavus in both cases is intriguing; the role of this organism in ingesta-associated choledocholithiasis and related sequelae in horses should be further investigated. We recommend routine assessment of the biliary tract for stones and bacterial culture in cases of both ingesta-associated and non–ingesta-associated cholelithiasis in horses to further characterize bacterial flora that contribute to these conditions.

Choledocholiths and choleliths associated with foreign material are not reported in other domestic species, although foreign material, such as sand and seeds, has been identified in the gallbladders of sheep and pigs, respectively.⁵ Anatomic features specific to the horse extrahepatic biliary tree, including the short length and wide caliber of the common bile duct as well as lack of a gallbladder, may contribute to the occurrence of this phenomenon in horses. Given the lack of a gallbladder, it is possible that there is more frequent opening of the major duodenal papilla for bile excretion, allowing a higher likelihood of ingesta ascending the common bile duct and predisposing to cholangitis and stone formation.

Foreign body–associated choleliths and choledocholiths in humans are most commonly associated with a history of biliary tract surgery with surgical sutures and Ligaclips (Ethicon) found within choleliths and choledocholiths.¹ Although uncommon, ingesta-associated choleliths and choledocholiths have been reported in humans in association with fish bones, toothpicks, tomato skin, and celery stalks.^{3,11,12,27,30} In these cases, biopsy and culture were not reported; however, these patients often had epigastric pain or icterus, and had evidence of common bile duct dilation with or without concurrent hepatic chemistry abnormalities.^11,12,27,30 In humans, although bacterial culture is not always performed, there is evidence that bacteria within the biliary tract have a role in stone formation, specifically given their ability to deconjugate bilirubin.^25,26 This reaction leads to the formation and aggregation of calcium bilirubinate “pigment” stones. In people, the risk of recurrent choledocholithiasis is associated with biliary tract infections and advanced age, both of which have also been reported in horses.^6,18

Although rare, we recommend assessment of the bile duct for the presence of choledocholiths in horses with persistent colic, pyrexia, and elevated hepatocellular and cholestatic biomarkers. Any retrieved choledocholiths should be evaluated for potential ingested foreign material that may serve as a nidus for choledocholith formation and ascending bacterial infection.

Supplemental Material

sj-pdf-1-vdi-10.1177_10406387231177251 – Supplemental material for Ingesta-associated choledocholithiasis in horses: 2 cases and literature review

Supplemental material, sj-pdf-1-vdi-10.1177_10406387231177251 for Ingesta-associated choledocholithiasis in horses: 2 cases and literature review by Sarah C. Linn-Peirano, Kate Hepworth-Warren, Hannah Kinsella, Dubraska Diaz-Campos, Bonnie M. Brenseke, Rachel E. Cianciolo, Eric Schroeder and Megan E. Schreeg in Journal of Veterinary Diagnostic Investigation

Footnotes

Acknowledgements

We thank and acknowledge the histology laboratory staff of North Carolina State University College of Veterinary Medicine as well as the Comparative Pathology and Digital Imaging Shared Resource of The Ohio State University College of Veterinary Medicine. In addition, we thank the North Carolina State University Clinical Microbiology and Molecular Diagnostics and The Ohio State University Diagnostic and Clinical Microbiology Service for their assistance with bacterial culture.

Declaration of conflicting interests

The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Funding

The authors received no financial support for the research, authorship, and/or publication of this article.

ORCID iDs

Sarah C. Linn-Peirano

Megan E. Schreeg

Supplemental material

Supplemental material for this article is available online.

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