Selenium (Se) is an essential trace element that is often deficient in the natural diets of domestic animal species. The measurement of Se in whole blood or liver is the most accurate way to assess Se status for diagnostic purposes. This study was conducted to compare hydride generation atomic absorption spectroscopy (HG-AAS) with inductively coupled plasma–mass spectrometry (ICP-MS) for the detection and quantification of Se in liver samples. Sample digestion was accomplished with magnesium nitrate and nitric acid for HG-AAS and ICP-MS, respectively. The ICP-MS detection was optimized for 82Se with yttrium used as the internal standard and resulted in a method detection limit of 0.12 μg/g. Selenium was quantified by both methods in 310 samples from a variety of species that were submitted to the Toxicology Laboratory at New Bolton Center (Kennett Square, PA) for routine diagnostic testing. Paired measurements for each sample were evaluated by a mean difference plot method. Limits of agreement were used to describe the maximum differences likely to occur between the 2 methods. Results suggest that under the specified conditions ICP-MS can be reliably used in place of AAS for quantitation of tissue Se at or below 2 μg/g to differentiate between adequate and deficient liver Se concentrations.
BekerDSatovicVVukelicN: 1994, Selenium content of chicken meat in Croatia. Nahrung38:267–272.
2.
BellangerJR: 1995, Adaptation of a dry ashing procedure for determination of selenium by fluorimetry in high-fat foods. J AOAC Int78:477–480.
3.
BlandJMAltmanDG: 1986, Statistical methods for assessing agreement between two methods of clinical measurement. Lancet1:307–310.
4.
BlandJAltmanDG: 1999, Measuring agreement in method comparison studies. Stat Methods Med Res8:135–160.
5.
BrumbaughWGWaltherMJ: 1991, Improved selenium recovery from tissue with modified sample decomposition. J Assoc Off Anal Chem74:570–571.
6.
BuckleyWTBudacJJGodfreyDVKoenigKM: 1992, Determination of selenium by inductively coupled plasma mass spectrometry utilizing a new hydride generation sample introduction system. Anal Chem64:724–729.
7.
BurgueraJLBurgueraMGallignaniMAlarconOM: 1990, A comparative study of methods for determining selenium in biological materials. Acta Cient Venez41:5–10.
8.
BurkRFLevanderOA: 1999, Selenium. In: Modern nutrition in health and disease, ed. ShilsMEOlsonJAShikeM, pp. 265–276. Williams and Wilkins, Baltimore, MD.
9.
CabreraCLorenzoMLLopezMC: 1995, Electrothermal atomic absorption spectrometric determination of cadmium, copper, iron, lead, and selenium in fruit slurry: analytical application to nutritional and toxicological quality control. J AOAC Int78:1061–1067.
10.
CaurantFNavarroMAmiardJC: 1996, Mercury in pilot whales: possible limits to the detoxification process. Sci Total Environ186:95–104.
11.
CedergrenALindbergILundbergE: 1986, Investigation of reactions involved in graphite furnace atomic absorption procedures. Anal Chim Acta180:378–388.
12.
DietzRRigetFBornEW: 2000, An assessment of selenium to mercury in Greenland marine animals. Sci Total Environ245:15–24.
13.
DoreaJGMoreiraMBEastGBarbosaAC: 1998, Selenium and mercury concentrations in some fish species of the Madeira River, Amazon Basin, Brazil. Biol Trace Elem Res65:211–220.
14.
DrakeENHainTD: 1994, Palladium(II), magnesium(II), and barium(II) nitrate combinations for matrix modification in electrothermal atomic absorption measurement of total selenium in human urine. Anal Biochem220:336–339.
15.
DraschGder MailSSchlosserCRoiderG: 2000, Content of non-mercury-associated selenium in human tissues. Biol Trace Elem Res77:219–230.
16.
EdwardsWCBlackburnTA: 1986, Selenium determination by Zeeman atomic absorption spectrophotometry. Vet Hum Toxicol28:12–13.
17.
EjimaAWatanabeCKoyamaH: 1996, Determination of selenium in the human brain by graphite furnace atomic absorption spectrometry. Biol Trace Elem Res54:9–21.
18.
EndoTHaraguchiKSakataM: 2002, Mercury and selenium concentrations in the internal organs of toothed whales and dolphins marketed for human consumption in Japan. Sci Total Environ300:15–22.
19.
FairrisGMLloydBDelvesHT: 1988, Skin selenium content measured by hydride generation and atomic absorption spectroscopy. J Trace Elem Electrolytes Health Dis2:181–184.
20.
FlanjakJ: 1978, Atomic absorption spectrometric determination of arsenic and selenium in offal and fish by hydride generation. J AOAC Int61:1299–1303.
21.
ForrerRGautschiKLutzH: 2001, Simultaneous measurement of the trace elements Al, As, B, Be, Cd, Co, Cu, Fe, Li, Mn, Mo, Ni, Rb, Se, Sr, and Zn in human serum and their reference ranges by ICP-MS. Biol Trace Elem Res80:77–93.
22.
ForrerRGautschiKStrohALutzH: 1998, Direct determination of selenium and other trace elements in serum samples by ICP-MS. J Trace Elem Med Biol12:240–247.
23.
ForrerRWenkerCHGautschiKLutzH: 2001, Concentration of 17 trace elements in serum and whole blood of plains viscachas (Lagostomus maximus) by ICP-MS, their reference ranges, and their relation to cataract. Biol Trace Elem Res81:47–62.
24.
FuavaoVAPorterBLSneddonJ: 1989, Direct determination of selenium in horse blood by graphite furnace atomic absorption spectroscopy. Anal Lett22:71–82.
25.
GiachettiCAssandriATestaB: 1996, Determination of selenium in plasma, urine and tissues of standard diet-fed rats and dogs by ETA-atomic absorption spectroscopy with Zeeman background correction. Boll Chim Farm135:494–501.
26.
HintzeKJLardyGPMarchelloMJFinleyJW: 2001, Areas with high concentrations of selenium in the soil and forage produce beef with enhanced concentrations of selenium. J Agric Food Chem49:1062–1067.
27.
HollisS: 1996, Analysis of method comparison studies. Ann Clin Biochem33:1–4.
28.
IndlisSStahleESchwartzJL, eds.: 2003, Compendium of veterinary products, 7th ed.North American Compendiums Inc., Port Huron, MI.
29.
IsaacRAIhnatM: 1995, Agricultural chemicals; contaminants; drugs. In: Official methods of analysis of AOAC international, ed. CunniffP, 16th ed., Chapter 3:20–21, Chapter 9: 1–44. AOAC International, Arlington, VA.
30.
JacobsonBELockitchG: 1988, Direct determination of selenium in serum by graphite-furnace atomic absorption spectrometry with deuterium background correction and a reduced palladium modifier: age-specific reference ranges. Clin Chem34:709–714.
31.
JanghorbaniMTingBTGLynchNE: 1989, Inductively coupled plasma mass spectrometry for stable isotope tracer investigations. Mikrochim Acta3:315–328.
32.
KeithLHCrummettWDeeganJJr: 1983, Principles of environmental analysis. Anal Chem55:2210–2218.
33.
KimEYSaekiKTanabeS: 1996, Specific accumulation of mercury and selenium in seabirds. Environ Pollut94:261–265.
34.
KivistoK: 1993, Misleading use of correlation and regression in method-comparison studies of cylcosporine. Clin Chem39:167–168.
35.
KoemanJHvan de VenWSMde GoeijJJM: 1975, Mercury and selenium in marine mammals and birds. Sci Total Environ3:279–287.
36.
KrausTQuidenusGSchallerKH: 2000, Normal values for arsenic and selenium concentrations in human lung tissue. Arch Environ Contam Toxicol38:384–389.
37.
KrushevskaAKotrebaiMLasztityA: 1996, Application of tertiary amines for arsenic and selenium signal enhancement and polyatomic interference reduction in ICP-MS analysis of biological samples. Fresenius J Anal Chem355:793–800.
38.
KumpulainenJRaittilaAMLehtoJKoivistoinenP: 1983, Electrothermal atomic absorption spectrometric determination of selenium in foods and diets. J AOAC Int66:1129–1135.
39.
LeonzioCFocardiSFossiC: 1992, Heavy metals and selenium in stranded dolphins of the Northern Tyrrhenian (NW Mediterranean). Sci Total Environ119:77–84.
40.
LewisSA: 1988, Determination of selenium in biological matrices. Methods Enzymol158:391–402.
41.
LinTHTsengWCChengSY: 1998, Direct determination of selenium in human blood plasma and seminal plasma by graphite furnace atomic absorption spectrophotometry and clinical application. Biol Trace Elem Res64:133–149.
42.
LindbergILundbergEArkhammarPBerggrenPO: 1988, Direct determination of selenium in solid biological materials by graphite furnace atomic absorption spectrometry. J Anal At Spectrom3:497–501.
43.
LourdesMACurvin-AralarAFurnessRW: 1991, Mercury and selenium interaction: a review. Ecotoxicol Environ Saf21:248–264.
44.
LozakASoltykKOstapczukPFijalekZ: 2002, Determination of selected trace elements in herbs and their infusions. Sci Total Environ289:33–40.
45.
MaasJParishSMHodgsonDR: 2002, Nutritional myodengeration. In: Large animal internal medicine, ed. SmithBP, pp. 1279–1282. Mosby, St. Louis, MO.
46.
MagariRT: 2002, Statistics for laboratory method comparison studies. BioPharm15:28–32.
47.
MartojaRBerryJP: 1980, Identification of tiemannite as a probable product of demethylation of mercury by selenium in cetaceans. A complement to the scheme of the biological cycle of mercury. Vie Milieu30:7–10.
48.
MatekMBlanusaMGrgicJ: 1999, Comparison of two methods using atomic absorption spectrometry for determination of selenium in food. Arh Hig Rad Toksikol50:283–288.
49.
McCurdyEPotterD: 2001, Optimizing ICP-MS for the determination of trace metals in high matrix samples. J Anal Atom Spectrom13:14–20.
50.
McLaugholinKDadgarDSmythMRMcMasterD: 1990, Determination of selenium in blood plasma and serum by flow injection hydride generation atomic absorption spectrometry. Analyst115:275–278.
51.
MendezHAlavaFLavillaIBendichoC: 2001, Determination of selenium in marine biological tissues by transverse heated electrothermal atomic absorption spectrometry with longitudinal Zeeman background correction and automated ultrasonic slurry sampling. J AOAC Int84:1921–1926.
52.
NuttallKGordonWHAshKO: 1995, Inductively coupled plasma mass spectrometry for trace element analysis in the clinical laboratory. Ann Clin Lab Sci25:264–271.
53.
OsterOPrellwitzW: 1982, A methodological comparison of hydride and carbon furnace atomic absorption spectroscopy for the determination of selenium in serum. Clin Chim Acta124:277–291.
54.
OsweilerGD: 1996, Metals and minerals. In: Toxicology, national veterinary medical series, ed. NieginskiEACannMDinkelAG, pp. 179–212. Williams and Wilkins, Philadelphia, PA.
55.
OttenbacherKTomchekS: 1994, Measurement variation in method comparison studies: an empirical examination. Arch Phys Med Rehabil75:505–512.
56.
PollockMJeffersonSKaneJ: 1992, Method comparison—a different approach. Ann Clin Biochem29:556–560.
57.
PooleCFEvansNJWibberleyDG: 1977, Determination of selenium in biological samples by gas-liquid chromatography with electron-capture detection. J Chromatogr136:73–83.
58.
PuchyrRFBassDAGajewskiR: 1998, Preparation of hair for measurement of elements by inductively coupled plasma-mass spectrometry (ICP-MS). Biol Trace Elem Res62:167–182.
59.
PulsR: 1994, Mineral levels in animal health: diagnostic data, 2nd ed.Sherpa International, Clearbrook, Canada.
60.
Rahil-KhazenRBolannBJMykingAUlvikRJ: 2002, Multi-element analysis of trace element levels in human autopsy tissues by using inductively coupled atomic emission spectrometry technique (ICP-AES). J Trace Elem Med Biol16:15–25.
61.
RothwellP: 2000, Analysis of agreement between measurements of continuous variables: general principles and lessons from studies of imaging of carotid stenosis. J Neurol415:825–834.
62.
ShibataYMoritaMFuwaK: 1992, Selenium and arsenic in biology: their chemical forms and biological functions. Adv Biophys28:31–80.
63.
SinghAKWhiteTArendtTJiangY: 1997, Analysis of selenium in bovine liver gas chromatography with mass-selective, electron-capture and nitrogen-phosphorus detection. J Chromatogr B690:327–331.
64.
SoledadSepulveda MPoppengaRHArrecisJJQuinnAB: 1998, Concentrations of mercury and selenium in tissues of double-crested cormorants (Phalacrocorax auritus) from Southern Florida. Colon Waterbirds21:35–42.
65.
StahrHM: 1991, Selenium in biological matrices and feed: atomic absorption spectroscopy. In: Analytical methods in toxicology, pp. 85–90. Wiley and Sons Inc., New York, NY.
66.
StockhamSLScottMA: 2002, Introductory concepts. In: Fundamentals of clinical pathology, pp. 3–28. Iowa State Press, Ames, IA.
67.
Subcommittee on Mineral Toxicity in Animals: 1980, Selenium. In: Mineral tolerances of domestic animals, pp. 392–420. National Academy of Sciences, National Academy Press, Washington, DC.
68.
SuzukiKTItohMOhmichiM: 1995, Detection of selenium-containing biological constituents by high-performance liquid chromatography-plasma source mass spectrometry. J Chromatogr B666:13–19.
69.
TinggiU: 1999, Determination of selenium in meat products by hydride generation atomic absorption spectrophotometry. J AOAC Int82:364–367.
70.
TracyMLMollerG: 1990, Continuous flow vapor generation for inductively coupled argon plasma spectrometric analysis. Part 1: selenium. J AOAC Int73:404–410.
71.
ValienteLPiccinnaMAleER: 2002, Determination of selenium in dietary supplements by ETAAS and HG-AAS: a comparative study. At Spectrosc23:129–134.
72.
van CauwenberghRRobberechtHDeelstraH: 1990, Selenium concentration levels in whole blood of Belgian blood bank donors as determined by direct graphite furnace atomic absorption spectrometry. J Trace Elem Electrolytes Health Dis4:215–224.
73.
WagemannRTrebaczEBoilaGLockhartWL: 1998, Methylmercury and total mercury in tissues of arctic marine mammals. Sci Total Environ218:19–31.
74.
WangHCPengHWKuoMS: 2001, Determination of beryllium and selenium in human urine and of selenium in human serum by graphite-furnace atomic absorption spectrophotometry. Anal Sci17:527–532.
75.
ZhouHLiuJ: 1997, The simultaneous determination of 15 toxic elements in foods by ICP-MS. At Spectrosc18:115–118.
