Abstract
This book is a very useful source that can be adopted for molecular biology and biotechnology courses. It is also an invaluable guide to researchers beginning work in those areas. Compared to other relevant texts, this book is unique in employing a single project-based approach in presenting the theory and practice in molecular biology techniques. It starts with experiments on creating a gene fusion between the glutathione-S-transferase (gst) gene and the enhanced green fluorescent protein (egfp) and proceeds with other lab work on gene expression and protein purification and quantification. This continued thread of successive, interrelated experiments allows students to well understand the rationale and practical aspects of molecular analyses. On completing this manual, readers will be well-trained on PCR, gene cloning, and recombinant DNA technology. The new fourth edition also includes additional chapters on modulating gene expression in mammalian cells using RNAi and CRISPR-Cas9 techniques.