The objective of this study was to determine the effects of hydrostatic pressure (HP) on the biochemical properties and gene expression of mesenchymal stem cells (MSCs) on scaffolds for cartilage tissue engineering composed of poly(caprolactone) (PCL) poly(vinyl alcohol) (PVA) gelatin (GEL) semi interpenetrating polymer network (semi-IPN). The MSCs were cultured on PCL-PVA-GEL semi-IPN scaffolds in two groups (A and B) for 7 and 21 days, respectively, and then loaded with hydrostatic pressure (5 MPa, 0.5 Hz) for 2 h per day for the period of 7 days and compared with two non-loaded groups (C and D) as controls. DMMB and real-time PCR analysis for assaying cartilage-specific extracellular matrix (ECM) gene markers were carried out. According to the results, there were no significant differences in GAG amounts between the loaded and non-loaded constructs were observed after 14 days. However, significant and considerable increases in the expression amount of type II collagen mRNA levels in group A (from 2.43 × 10−4 ± 5.32 × 10−5 to 2.09 × 10−3 ± 1.07 × 10−4 time), and in group B (from 3.04 × 10−4 ± 4.31 × 10−5 to 2.08 × 10−3 ± 1.59 × 10−4 time) in comparison with non-loaded groups (C and D) were observed, respectively. Results showed the beneficial role of hydrostatic pressure on the increase of type II collagen mRNA levels in articular cartilage tissue engineering.
OgawaR., MizunoS., MurphyG.F., OrgillD.P.The effect of hydrostatic pressure on three-dimensional chondroinduction of human adipose-derived stem cells. Tissue Eng Part A.2009; 15(10): 2937–2945.
LundA.W., YenerB., StegemannJ.P., PlopperG.E.The natural and engineered 3D microenvironment as a regulatory cue during stem cell fate determination. Tissue Eng Part B Rev.2009; 15(3): 371–380.
5.
GuilakF., CohenD.M., EstesB.T., GimbleJ.M., LiedtkeW., ChenChS. Control of Stem Cell Fate by Physical Interactions with the Extracellular Matrix. Cell Stem Cell.2009; 5(1): 17–26.
6.
OgawaR.Ogawa. Recent Patents on Stem Cell-Mediated Cartilage Regeneration and Repair. Recent Patents on Regenerative Medicine.2013; 3(1-1); 118–122.
7.
CandianiG., RaimondiM.T., AuroraR., Lagana’K., DubiniG.Chondrocyte response to high regimens of cyclic hydrostatic pressure in 3-dimensional engineered constructs. Int J Artif Organs.2008; 31(6): 490–499.
8.
MeyerE.G., BuckleyC.T., StewardA.J., KellyD.J.The effect of cyclic hydrostatic pressure on the functional development of cartilaginous tissues engineered using bone marrow derived mesenchymal stem cells. J Mech Behav Biomed Mater.2011; 4(7): 1257–1265.
9.
KellyD.J., JacobsC.R.The role of mechanical signals in regulating chondrogenesis and osteogenesis of mesenchymal stem cells. Birth Defects Res C Embryo Today.2010; 90(1): 75–85.
10.
ToyodaT., SeedhomB.B., YaoJ.Q., KirkhamJ., BrookesS., BonassW.A.Hydrostatic pressure modulates proteoglycan metabolism in chondrocytes seeded in agarose. Arthritis Rheum.2003; 48(10): 2865–2872.
11.
GunjaN.J., AthanasiouK.A.Effects of hydrostatic pressure on leporine meniscus cell-seeded PLLA scaffolds. J Biomed Mater Res A.2010; 92(3): 896–905.
12.
BonakdarSh.Hojjati Emami Sh, Shokrgozar MA, Farhadi A, Hoshiar Ahmadi SA, Amanzadeh A. Preparation and characterization of polyvinyl alcohol hydrogels crosslinked by biodegradable polyurethane for tissue engineering of cartilage. Mater Sci Eng C.2010; 30(4): 636–643.
13.
LeeJ.H., ParkJ.W., LeeH.B.Cell adhesion and growth on polymer surfaces with hydroxyl groups prepared by water vapour plasma treatment. Biomaterials.1991; 12(5): 443–448.
14.
MatsikoA., LevingstoneT.J., O'BrienF.J., GleesonJ.P.Addition of hyaluronic acid improves cellular infiltration and promotes early-stage chondrogenesis in a collagen-based scaffold for cartilage tissue engineering. J Mech Behav Biomed Mater.2012; 11: 41–52.
15.
LiZ., YaoS.J., AliniM., StoddartM.J.Chondrogenesis of human bone marrow mesenchymal stem cells in fibrin-polyurethane composites is modulated by frequency and amplitude of dynamic compression and shear stress. Tissue Eng Part A.2010; 16(2): 575–584.
16.
BlumbergT.J., NatoliR.M., AthanasiouK.A.Effects of doxycycline on articular cartilage GAG release and mechanical properties following impact. Biotechnol Bioeng.2008; 100(3): 506–515.
17.
HaughM.G., MurphyC.M., McKiernanR.C., AltenbuchnerC., O'BrienF.J.Crosslinking and mechanical properties significantly influence cell attachment, proliferation, and migration within collagen glycosaminoglycan scaffolds. Tissue Eng Part A.2011; 17(9-10): 1201–1208.
18.
BianL., LimaE.G., AngioneS.L.. Mechanical and biochemical characterization of cartilage explants in serum-free culture. J Biomech.2008; 41(6): 1153–1159.
19.
HunterC.J., MouwJ.K., LevenstonM.E.Dynamic compression of chondrocyte-seeded fibrin gels: effects on matrix accumulation and mechanical stiffness. Osteoarthr. Cartil.2004; 12(2): 117–130.
20.
NerurkarN.L., SenS., BakerB.M., ElliottD.M., MauckR.L.Dynamic culture enhances stem cell infiltration and modulates extracellular matrix production on aligned electrospun nanofibrous scaffolds. Acta Biomater.2011; 7(2): 485–491.
LutolfM.P., GilbertP.M., BlauH.M.Designing materials to direct stem-cell fate. Nature.2009; 462(7272): 433–441.
23.
ShinH.J., LeeC.H., ChoI.H.. Electrospun PLGA nanofiber scaffolds for articular cartilage reconstruction: mechanical stability, degradation and cellular responses under mechanical stimulation in vitro. J Biomater Sci Polym Ed.2006; 17(1-2): 103–119.
24.
ZhangY., OuyangH., LimCh, RamakrishnaS., HuangZ.M.Electrospinning of Gelatin Fibers and Gelatin/PCL Composite Fibrous Scaffolds. J Biomed Mater Res B Appl Biomater.2005; 72B(1): 156–165.
BinanL., TendeyCh, De CrescenzoG., El AyoubiR., AjjiA., JolicoeurM.Differentiation of neuronal stem cells into motor neurons using electrospun poly-L-lactic acid/gelatin scaffold. Biomaterials.2014; 35(2): 664–674.
27.
KołbukD., SajkiewiczP., Maniura-WeberK., FortunatoG.Structure and morphology of electrospun polycaprolactone/gelatine nanofibres. Eur Polym J.2013; 49: 2052–2061.
28.
HardinghamT.E., OldershawR.A., TewS.R.Cartilage, SOX9 and Notch signals in chondrogenesis. J Anat.2006; 209(4): 469–480.
29.
VinatierC., MrugalaD., JorgensenC., GuicheuxJ., NoëlD.Cartilage engineering: a crucial combination of cells, biomaterials and biofactors. Trends Biotechnol.2009; 27(5): 307–314.
30.
WagnerD.R., LindseyD P., LiK.W.. Hydrostatic pressure enhances chondrogenic differentiation of human bone marrow stromal cells in osteochondrogenic medium. Ann Biomed Eng.2008; 36(5): 813–820.
31.
IkenoueT., TrindadeM.C., LeeM.S.. Mechanoregulation of human articular chondrocyte aggrecan and type II collagen expression by intermittent hydrostatic pressure in vitro. J Orthop Res.2003; 21(1): 110–116.
