Ramadan fasting involves refraining from food and liquids from the sunrise till sunset for one month yearly; affecting the sleep pattern and duration, physical activity, and meal number and composition leading to biochemical and physiological changes in the body. Irisin, adiponectin, and leptin induce multiple metabolic effects.
OBJECTIVE:
The aim of this study is to examine the effect of the Ramadan fasting on serum levels of irisin, adiponectin, and leptin.
MATERIALS AND METHODS:
A total of sixty healthy students (43 females and 17 males) were recruited, 20.9 years mean age, who were fasting for Ramadan were included in this study. Anthropometry, blood samples and physical activity were determined. Lipid profiles were determined using an enzymatic colorimetric method, and serum levels of irisin, adiponectin, and leptin were measured using Enzyme-linked Immunosorbent Assay. All measurements were measured before Ramadan and in the fourth week of Ramadan.
RESULTS:
Subjects experienced a significant increase in LDL was determined during Ramadan vs before Ramadan (142.8 vs. 158.1 mg/dl p = 0.03) along with a significant reduction in leptin, adiponectin and irisin concentration during Ramadan compared to before Ramadan (11.6 vs. 8.6; p = 0.000; 4.42 vs. 3.58; p = 0.022; 33.6 vs. 16.3; p = 0.003). A significant positive correlation was found between physical activity and irisin concentration before Ramadan (r = 0.338, p = 0.022).
CONCLUSIONS:
Ramadan fasting has significant effects on irisin, adiponectin, and leptin levels. Their levels were significantly reduced during Ramadan while LDL level was increased. Irisin level has a significant correlation with the physical activity of the participants.
KulS, SavasE, OzturkZA, KaradagG. Does Ramadan fasting alter body weight and blood lipids and fasting blood glucose in a healthy population? A meta-analysis. J Relig Health. 2014;53(3):929–42. 10.1007/s10943-013-9687-0
2.
GnanouJV, CaszoBA, KhalilKM, AbdullahSL, KnightVF, BidinMZ. Effects of Ramadan fasting on glucose homeostasis and adiponectin levels in healthy adult males. J Diabetes Metab Disord. 2015;14:55. 10.1186/s40200-015-0183-9
3.
Al-HouraniHM, AtoumMF. Body composition, nutrient intake and physical activity patterns in young women during Ramadan. Singapore Med J. 2007;48(10):906–10.
4.
KiyaniMM, MemonAR, AmjadMI, AmeerMR, SadiqM, MahmoodT. Study of Human Biochemical Parameters During and After Ramadan. J Relig Health. 2017;56(1):55–62. 10.1007/s10943-015-0084-8
5.
KhattakM, MamatN, Abu HakerW, ShaharuddinM. Does religious fasting affect energy and macro-nutrients intakes?Nutrition & Food Science. 2013;43(3):7. DOI 10.1108/00346651311327909
6.
PrasetyaG, SapwarobolS. Intermittent Fasting During Ramadan Improves Insulin Sensitivity and Anthropometric Parameters in Healthy Young Muslim Men. American Journal of Lifestyle Medicine. 2018;12. DOI: 10.1177/1559827618815430.
7.
AlzoghaibiMA, Pandi-PerumalSR, SharifMM, BaHammamAS. Diurnal intermittent fasting during Ramadan: The effects on leptin and ghrelin levels. PLoS One. 2014;9(3):e92214. 10.1371/journal.pone.0092214
8.
AjabnoorGM, BahijriS, BoraiA, AbdulkhaliqAA, Al-AamaJY, ChrousosGP. Health impact of fasting in Saudi Arabia during Ramadan: Association with disturbed circadian rhythm and metabolic and sleeping patterns. PLoS One. 2014;9(5):e96500. 10.1371/journal.pone.0096500
9.
RabieeS, AfghariN, RastmaneshR. Short-term and mid-term effects of fasting and downset meal pattern on lipid profile in Iranian fasted women. J Relig Health. 2014;53(3):654–7. 10.1007/s10943-012-9659-9
10.
KhafajiHA, BenerA, OsmanM, Al MerriA, Al SuwaidiJ. The impact of diurnal fasting during Ramadan on the lipid profile, hs-CRP, and serum leptin in stable cardiac patients. Vasc Health Risk Manag. 2012;8:7–14. 10.2147/VHRM.S22894
11.
TrabelsiK, El AbedK, TrepanowskiJF, StannardSR, GhlissiZ, GhozziH, et al.Effects of ramadan fasting on biochemical and anthropometric parameters in physically active men. Asian J Sports Med. 2011;2(3):134–44.
12.
UnalacakM, KaraIH, BaltaciD, ErdemO, BucaktepePG. Effects of Ramadan fasting on biochemical and hematological parameters and cytokines in healthy and obese individuals. Metab Syndr Relat Disord. 2011;9(2):157–61. 10.1089/met.2010.0084
13.
AziziF. Islamic fasting and health. Ann Nutr Metab. 2010;56(4):273–82. 10.1159/000295848
14.
HaghdoostAA, PoorranjbarM. The interaction between physical activity and fasting on the serum lipid profile during Ramadan. Singapore Med J. 2009;50(9):897–901.
15.
Lamri-SenhadjiMY, El KebirB, BellevilleJ, BouchenakM. Assessment of dietary consumption and time-course of changes in serum lipids and lipoproteins before, during and after Ramadan in young Algerian adults. Singapore Med J. 2009;50(3):288–94.
16.
BouhlelE, DenguezliM, ZaoualiM, TabkaZ, ShephardRJ. Ramadan fastings effect on plasma leptin, adiponectin concentrations, and body composition in trained young men. Int J Sport Nutr Exerc Metab. 2008;18(6):617–27.
17.
LessanN, SaadaneI, AlkafB, HamblyC, BuckleyAJ, FinerN, et al.The effects of Ramadan fasting on activity and energy expenditure. Am J Clin Nutr. 2018;107(1):54–61. 10.1093/ajcn/nqx016
18.
BoströmJ, WMP, JA, KL Y, JCL, et al.A PGC1-α-dependent myokine that drives brown-fat-like development of white fat and thermogenesis. Nature. 2012;481:6. doi: 10.1038/nature10777
19.
AydinS, KulogluT, AydinS, KalayciM, YilmazM, CakmakT, et al.A comprehensive immunohistochemical examination of the distribution of the fat-burning protein irisin in biological tissues. Peptides. 2014;61:130–6. 10.1016/j.peptides.2014.09.014
20.
Moreno-NavarreteF, OM, SE, GG, PFT, et al.Irisin is expressed and produced by human muscle and adipose tissue in association with obesity and insulin resistance. J Clin Endocrinol Metab. 2013;98(4):10. doi: 10.1210/jc.2012-2749
21.
UkkolaVP, POP, PJT, NES, LMP T, et al.High serum fasting peptide YY (3-36) is associated with obesity-associated insulin resistance and type 2 diabetes. Regul Pept. 2011;170:5. doi: 10.1016/j.regpep.2011.05.006
22.
RenaldiO, PramonoB, SinoritaH, PurnomoLB, AsdieRH, AsdieAH. Hypoadiponectinemia: A risk factor for metabolic syndrome. Acta Med Indones. 2009;41(1):20–4.
23.
AritaY, KiharaS, OuchiN, TakahashiM, MaedaK, MiyagawaJ, et al.Paradoxical decrease of an adipose-specific protein, adiponectin, in obesity. Biochem Biophys Res Commun. 1999;257(1):79–83.
24.
SprangerJ, KrokeA, MohligM, BergmannMM, RistowM, BoeingH, et al.Adiponectin and protection against type 2 diabetes mellitus. Lancet. 2003;361(9353):226–8. 10.1016/S0140-6736(03)12255-6
PanH, GuoJ, SuZ. Advances in understanding the interrelations between leptin resistance and obesity. Physiol Behav. 2014;130:157–69. 10.1016/j.physbeh.2014.04.003
27.
MartinLJ, WooJG, GeraghtySR, AltayeM, DavidsonBS, BanachW, et al.Adiponectin is present in human milk and is associated with maternal factors. Am J Clin Nutr. 2006;83(5):1106–11. 10.1093/ajcn/83.5.1106
28.
FeizollahzadehS, RasuliJ, KheirouriS, AlizadehM. Augmented plasma adiponectin after prolonged fasting during ramadan in men. Health Promot Perspect. 2014;4(1):77–81. 10.5681/hpp.2014.010
29.
KhoshdelA, KheiriS, NasiriJ, TehranHA, HeidarianE. The effect of Ramadan fasting on serum leptin, neuropeptide Y and insulin in pregnant women. Med J Islam Repub Iran. 2014;28:92.
30.
KassabS, Abdul-GhaffarT, NagallaDS, SachdevaU, NayarU. Interactions between leptin, neuropeptide-Y and insulin with chronic diurnal fasting during Ramadan. Ann Saudi Med. 2004;24(5):345–9. 10.1093/ajcn/37.3.461
31.
BouchardC, TremblayA, LeblancC, LortieG, SavardR, TheriaultG. A method to assess energy expenditure in children and adults. Am J Clin Nutr. 1983;37(3):461–7.
32.
AllainCC, PoonLS, ChanCS, RichmondW, FuPC. Enzymatic determination of total serum cholesterol. Clin Chem. 1974;20(4):470–5.
33.
FossatiP, PrencipeL. Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide. Clin Chem. 1982;28(10):2077–80.
34.
FriedewaldWT, LevyRI, FredricksonDS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem. 1972;18(6):499–502.
35.
FAO/WHO/UNU/ExpertConsultation. Human Energy Requirements. Rome; 2001.
36.
Ismail, Haron. Effect of Ramadan Fasting on Serum Lipid Profile among Healthy Students in UiTM. International Conference on Biological, Chemical and Environmental Sciences. 2014:3. doi: 10.15242/IICBE.C614058
37.
ZiaeeV, RazaeiM, AhmadinejadZ, ShaikhH, YousefiR, YarmohammadiL, et al.The changes of metabolic profile and weight during Ramadan fasting. Singapore Med J. 2006;47(5):409–14.
38.
AkaberiA, GolshanA, MoojdekanlooM, HashemianM. Does fasting in Ramadan ameliorate Lipid profile? A prospective observational study. Pak J Med Sci. 2014;30(4):708–11.
39.
GrundySM. Comparison of monounsaturated fatty acids and carbohydrates for lowering plasma cholesterol. N Engl J Med. 1986;314(12):745–8. 10.1056/NEJM198603203141204
40.
HuhJY, MougiosV, KabasakalisA, FatourosI, SiopiA, DouroudosII, et al.Exercise-induced irisin secretion is independent of age or fitness level and increased irisin may directly modulate muscle metabolism through AMPK activation. J Clin Endocrinol Metab. 2014;99(11):E2154–61. 10.1210/jc.2014-1437
41.
BiniaminovN, BandtS, RothA, HaertelS, NeumannR, BubA. Irisin, physical activity and fitness status in healthy humans: No association under resting conditions in a cross-sectional study. PLoS One. 2018;13(1):e0189254. 10.1371/journal.pone.0189254
