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Abstract

Ovarian follicular atresia in all vertebrates is mediated via apoptosis that is initiated in the granulosa cell layer. Here we investigated the relation between connexin expression, cell coupling, and apoptosis in avian granulosa cells. Results from qualitative and quantitative immunocytochemical analysis and Western blotting of connexin43 (Cx43) and electron microscopic observations of gap junctions were compared with functional data on gap junctional coupling obtained by fluorescence recovery after photobleaching in four experimental groups: a control group of freshly isolated granulosa cells, 24-hr serum-free cultures as the apoptosis-inducing condition, and two other groups in which apoptosis was inhibited by either hormone substitution or exposure to elevated extracellular calcium. Our work shows that apoptosis induction in granulosa cells is accompanied by an increased level of cell coupling and that decreasing cell coupling with the gap junction blocker α-glycyrrhetinic acid dose-dependently inhibits apoptosis. The level of Cx43 expression was inversely related to the apoptotic index, suggesting that Cx43 expression plays a role in granulosa cell survival. Our study supports the hypothesis that gap junctional coupling plays a role in propagating a cell death message and suggests a role for Cx43 expression per se in granulosa cell survival.

Keywords

granulosa cells apoptosis gap junctions connexin43 FRAP

The majority of the female germ line dies throughout postnatal life, because most follicles never ovulate but rather degenerate by a process called follicular atresia. It has been established that apoptotic cell death of granulosa cells is instrumental in the process of follicular atresia in the ovary of all vertebrates, including humans (Tilly 1997; Jiang et al. 2003). Although the vast majority of research to elucidate the molecular ordering of cell signaling during the process of granulosa cell apoptosis has been conducted in mammalian model systems, there is ample evidence to demonstrate that many of the proteins, enzymes, and cell signaling pathways are common to ovarian follicles from avian species (Johnson 2000).

Most studies on apoptosis regulation, including ours, are focusing predominantly on intracellular signaling pathways (D'Herde et al. 2000; Krysko et al. 2001), while tissue function obviously involves cell-to-cell communication. Granulosa cells can communicate either through local production of intraovarian factors such as cytokines (Adashi 1992) and growth factors (Adashi et al. 1991), which act as paracrine and/or autocrine modulators, or through gap junctions. Gap junctions are channels formed by two hemichannels, each formed by six connexin subunits contributed by each cell. All known connexins are transmembrane proteins with four transmembrane domains, two extracellular loops, one intracellular loop, and cytoplasmic amino and carboxyl termini (Bruzzone et al. 1996; Munari-Silem and Rousset 1996; Trosko and Ruch 1998). Cx43 is the major gap junctional protein expressed in granulosa cells that makes a significant contribution to intercellular coupling (Dekel 1987; Grazul-Bilska et al. 1997; Farioli-Vecchioli et al. 2000; Sommersberg et al. 2000; Rosenfeld et al. 2001; Gittens et al. 2003). It has been shown that gap junctions play an important role in granulosa cell development, differentiation, and luteinization (Kidder and Mhawi 2002). Classically, it is believed that gap junctions provide a way for cell-to-cell diffusion of hydrophilic molecules with a molecular mass of less than 1 kD, such as cAMP, Ca²⁺, IP3, and ATP (Kumar and Gilula 1996). More recently, however, several studies demonstrated that Cx43 could affect signaling pathways independently of its contribution to gap junction channels (Moorby and Patel 2001; Plotkin and Bellido 2001). Despite the increased interest of research in this field, little is known regarding the involvement of gap junctional intercellular communication and Cx43 expression during apoptosis. In a previous study, we observed by electron microscopy (EM) in 24-hr serum-free cultured granulosa cell explants, in which apoptosis is elicited by gonadotropin withdrawal, an increased number of huge gap junctions in comparison with uncultured granulosa cells (D'Herde and Leybaert 1997). This observation prompted us to investigate the relation between connexin expression, cell coupling, apoptosis, and survival of the granulosa cell explants.

Materials and Methods

Isolation and Culture of Granulosa Cell Sheets

Granulosa cell sheets were prepared from ovarian follicles of regularly laying adult Japanese quails (Coturnix coturnix japonica). The animals were reared under an artificial light cycle of 18 hr per day, with food (fresh lettuce and complete breeding food; Biofor AVEVE, Belgium) and water ad libitum. Animal care procedures were conducted in accordance with the guidelines set by the local ethical committee ECP 00/05 (2000). The monolayered granulosa epithelium of the largest preovulatory follicle (F1) was isolated in Krebs-Ringer solution as previously described (Gilbert et al. 1977). This method provides large sheets of vital granulosa cells sandwiched between their basement and vitelline membranes (Mussche et al. 2000; Krysko et al. 2001). The granulosa explants are cultured in suspension in phenol red-free M199 medium (Sigma; Bornem, Belgium) supplemented with 0.1% w/v bovine serum albumin (BSA) fraction V (Sigma), 6.0 g/liter HEPES (Acros; Geel, Belgium), 1% v/v penicillin-streptomycin (GIBCO; Paisley, UK) at pH 7.4. To inhibit the apoptotic process, the culture medium of controls was supplemented either with a combination of luteinizing hormone (LH, 100 ng/ml; Sigma-Aldrich, Bornem, Belgium) and insulin-like growth factor-I (IGF-I, 10 ng/ml; Sigma-Aldrich; (Onagbesan and Peddie 1988; Krysko et al. 2001) or with a continuous load of increased extracellular Ca²⁺, a protocol that promotes survival through activation of a calcium sensing receptor (Mussche et al. 2000; and Mussche et al. unpublished data). The concentration of all vehicle solutions was always below 0.1% and had no effects on the viability of granulosa cell explants (data not shown).

Quantification of Apoptosis by DAPI Staining

Granulosa cell explants were rinsed in 10 mM PBS, pH 7.4, and fixed in 4% buffered formaldehyde. Thereafter, granulosa cell explants were rinsed again in PBS and stained with a 1:1000 solution of DAPI (2',6'-diamidino-2-phenylindole; Sigma-Aldrich) in PBS. Mounted granulosa cell explants were examined in a Leica DM IRB/E inverted microscope and apoptotic cells were identified by their characteristic fragmented and condensed chromatin masses. Small groups of apoptotic bodies were counted as remnants of one apoptotic cell. Apoptotic indexes (AIs) are expressed as the number of apoptotic nuclei per number of total nuclei counted in the same microscopic field. This AI was averaged for 10 fields, giving a total number of ≃1500 cells counted per experiment.

Antibodies

A mouse monoclonal antibody (IgG1) raised against the peptide sequence corresponding to the amino acid residues 252–270 of the native rat Cx43 was used for immunocytochemistry (ICC) and Western blotting and was purchased from ProBio (Poole, UK) and BD Biosciences (Erembodegem, Belgium), respectively. These monoclonal antibodies were reported to crossreact with avian Cx43 (Beyer 1990; Musil et al. 1990). Affinity-purified secondary goat anti-mouse (IgG) antibody conjugated to horseradish peroxidase (HRP) were obtained from Cell Signaling Technology (Westburg, Leusden, The Netherlands) and secondary rabbit-FITC anti-mouse (IgG) antibody was purchased from DAKO (Glostrup, Denmark).

Immunocytochemistry of Connexin43

After rinsing of granulosa cell explants with PBS, they were fixed for 20 min in 3% PBS-buffered paraformaldehyde. Next, explants were incubated for 20 min in 3% BSA and then for 10 min in 1% glycine. After 45-min incubation in 10% normal rabbit serum, the explants were incubated, directly and without a rinsing step, overnight at 4C with mouse monoclonal anti-Cx43 antibody (1:400 dilution of 1 mg/ml stock; or for the controls the corresponding IgG1 at the same concentration; DAKO). Then the granulosa explants were incubated for 45 min with rabbit-FITC anti-mouse IgG (1:40). For double staining, the granulosa explants were stained with DAPI as described above, rinsed with 0.9% NaCl, and mounted with fluorescent mounting medium. Unless otherwise described, explants were rinsed thoroughly with PBS between all steps.

Quantification of Cx43 Immunofluorescence

Quantitative measurements of Cx43 immunofluorescence (Laing et al. 1997) were performed by determining the average fluorescence intensity in images obtained by confocal laser scanning microscopy (CLSM) of granulosa cell sheets or explants immunostained as described above. Images were acquired with a custom-made video-rate CLSM (Sanderson and Parker 2003) built around a Nikon Eclipse TE300 (Analis; Ghent, Belgium) equipped with a ×40 oil immersion objective (CFI Plan Fluor; Nikon) and with 488-nm excitation from an argon laser.

Protein Extraction and Western Blotting Analysis of Cx43 Expression

Granulosa cell explants were lysed in 50 μl of ice-cold modified RIPA buffer (50 mM NaCl, 0.5% NP40, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate, 250 mM α-glycerophosphate, 25 mM Tris, pH 8.2) containing a standard phosphatase inhibitor cocktail 2 (20 μl/ml; Sigma-Aldrich) and complete EDTA-free protease inhibitor tablet (20 μl/ml; Roche Diagnostics, Brussels, Belgium) for 10 min and sonicated on ice three times for 10 sec using a Vibracell VC-130 sonicator. The protein concentration of each sample was measured according to Bradford (1976) using the BioRad protein assay kit (BioRad Laboratories; Nazareth/Eke, Belgium) and a microtiter plate reader model 550 (Bio-Rad Laboratories). Protein samples were solubilized in Laemlli sample buffer and were heated at 100C for 10 min and centrifuged at 13,000 × g for 7 min to remove cell debris. Rat brain lysate was obtained from BD Biosciences (Erembodegem, Belgium) and was used as a positive control for Cx43 protein expression on Western blots. Approximately 55 μg of total protein from each sample was separated on 12% Tris-HCl precast gels (BioRad Laboratories). After separation, proteins were transferred to a nitrocellulose membrane (VWR; Zaventem, Belgium), blocked for 1 hr in TBS containing 5% non-fat dried milk and 0.1% Tween (blocking buffer), and incubated overnight at 4C with monoclonal antibodies to Cx43 diluted 1:250 in blocking buffer. After extensive washes in blocking buffer, membranes were probed for 1 hr with HRP-conjugated secondary goat anti-mouse IgG antibody diluted in blocking buffer (1:2000) and developed by enhanced chemiluminescence (Cell Signaling Technology; Westburg) according to the manufacturer's instructions. The time of exposure (typically 10 min) to X-ray films (Kodak Biomax Light-1; Sigma-Aldrich) was identical for all experimental conditions. The intensity of the bands after Western blotting was determined by scanning of the X-ray films followed by quantitative analysis using Total-lab software (Phoretix).

Transmission Electron Microscopy

The granulosa explants were fixed by immersion for 2 hr in 2% glutaraldehyde buffered with 0.1 M Na-cacodylate containing 1 mM CaCl₂ (pH 7.4). After a rinse in 0.1 M Nacacodylate containing 7.5% sucrose, the explants were osmicated in 2% OsO₄ in 0.1 M Na-cacodylate and embedded in LX medium (Ladd; Burlington, VT). Ultrathin sections were stained with uranyl acetate and lead citrate and were analyzed on a Jeol 1200 EXII electron microscope.

Fluorescence Recovery After Photobleaching (FRAP)

Gap junctional coupling was investigated in granulosa cell sheets or explants using the FRAP technique (Braet et al. 2003). For this purpose, the sheets or explants were loaded with the gap junction-permeable fluorescent tracer 5-carboxy-fluorescein diacetate (CFDA; Molecular Probes, Leiden, The Netherlands) by incubation in Hank's buffered salt solution (HBSS-HEPES) containing 50 μM CFDA for 60 min at room temperature and under continuous shaking. The preparations were then rinsed three times with HBSS-HEPES and transferred to the microscope stage of the CLSM described above. The loading and rinsing solutions contained in addition 1 mM probenecid (Sigma) to prevent rapid dye extrusion from the cells (Di Virgilio et al. 1988; D'Herde and Leybaert 1997). Fluorescence within a single cell was photobleached by spot exposure to the 488-nm line of an argon laser over a period of 10 sec. Spot exposure was done by reducing the scan amplitude of the horizontal and vertical scanning mirrors. CFDA fluorescence before and after photobleaching was recorded in confocal imaging mode with normal scanning mirror settings. Recovery of fluorescence because of dye influx from neighboring cells was quantified at 4 min after the start of photobleaching. A correction was made for the baseline bleaching effect associated with repeated exposure to the 488-nm imaging light. This was done by recording the fluorescence profile in cells not exposed to the spot bleach and correcting the recovery signal in proportion to the baseline fluorescence decay.

Gap Junction Blocking by 18 α-Glycyrrhetinic Acid

Eighteen α-glycyrrhetinic acid (3 α -hydroxy-11-oxo-18 α, 20 α -olean-12-en-29-oic acid, AGA; Sigma) was dissolved in dimethylsulfoxide (DMSO; UCB, Leuven, Belgium). To study the effect of gap junction blocking on cell survival, granulosa cell explants were incubated for 24 hr with AGA doses ranging from 25 μM to 250 μM and AIs were calculated on DAPI- stained granulosa cell explants as described above. Cell coupling after treatment with AGA (250 μM, 24 hr) was investigated using FRAP.

Statistical Analysis

The data are expressed as mean ± SEM, with n denoting the number of experiments. Statistical significance was tested using a t-test for unpaired observations and using a p value of less than 0.05. Multiple groups were compared using variance analysis, followed by the Dunnett test for multiple comparisons to a control group or the Student-Newman-Keuls test for comparison of all groups among each other. Curve fittings for dose–response relations were performed with non-linear least-square procedures available in the program Inplot.

Figure 1.

Effect of the culture conditions on the AI in granulosa cell explants. Culturing granulosa cell explants under 24h-SF condition induced apoptosis, while culturing granulosa cell explants for 24h-SF-H or 24h-SF-Ca supplemented conditions inhibited this induced apoptosis. The AI in fresh granulosa cell sheets was negligible and therefore is not shown. ∗p<0.05 compared with 24h-SF; n = 60 in six different animals.

Results

Apoptosis and Cx43 ICC

Freshly isolated preparations were used for control purposes, 24-hr serum-free (24h-SF) cultures as the apoptosis-inducing condition (D'Herde and Leybaert 1997), and 24h-SF cultures supplemented either with hormones (IGF-I and LH; 24h-SF-H) (Onagbesan and Peddie 1988; Krysko et al. 2001) or with an increased concentration of extracellular calcium (24h-SF-Ca) (Mussche et al. 2000; and Mussche et al. unpublished data) as the apoptosis-inhibiting conditions. The AIs observed under these experimental conditions are illustrated in Figure 1.

Staining of freshly isolated granulosa cell sheets with anti-Cx43 antibodies demonstrated immunoreactivity appearing as a fine and continuous trace, lining the cell borders and involving both places of cell-cell contact as well as non-junctional regions (Figures 2A and 2E). Granulosa cell explants in 24h-SF cultures displayed gap junctional plaques in the form of large dots or lines at cell-cell interfaces (Figures 2B, 2F, and 2I). On average, each cell contained 5.24 ± 0.11 (n=100 cells) of these immunopositive dots or lines. Apoptotic cells, easily recognized by their nuclear morphology by DAPI staining, did not show Cx43 immunolabeling at their plasma membrane (Figure 2I). However, occasionally apoptotic bodies demonstrated very weak dot-like cytoplasmic staining (Figure 2I). Interestingly, DAPI staining revealed apoptotic granulosa cells that were lying in clusters or streaks (Figure 2J). Granulosa cell explants in 24h-SF-H culture displayed a staining pattern that was regular and more intense than in 24h-SF culture condition. The gap junctions appeared to be huge as well (Figures 2C and 2G) with on average 6.30 ± 0.14 (n=100 cells) of these gap junctional immunopositivities per cell (significantly higher compared with 24h-SF granulosa cell explants). In granulosa cell explants cultured under 24h-SF-Ca conditions, the immunostaining appeared as a dotted pattern of many short gap junctions surrounding each cell (Figures 2D and 2H). Quantification of the level of Cx43 immunoreactivity, determined from the average intensity of confocal images acquired in the four conditions, demonstrated that the immunoreactivity in 24h-SF granulosa cell explants was significantly below the level observed in freshly isolated granulosa cells. The Cx43 immunoreactivity was significantly higher under the 24h-SF-H and 24h-SF-Ca, i.e., apoptosis-inhibiting conditions, compared with 24h-SF (Figure 3A).

Figure 2.

Epifluorescence micrographs of granulosa cells. (A–D) Stained with anti-Cx43 antibody alone or (E–I) in combination with DAPI. (J) Stained with DAPI alone. (A,E) In freshly isolated granulosa cell sheets, gap junctions appear as a fine and continuous line bordering the cells. Immunoreactivity covers both junctional and non-junctional regions. Cx43 immunoreactivity is also present in the cytoplasm. (B,F,I) In granulosa cell explants cultured under 24h-SF conditions, gap junctions appear as big dots or lines and are less apparent in the cytoplasm. (I) Note apoptotic cells with absence of Cx43 immunolabeling (arrows). However, occasionally Cx43 immunoreactivity in the cytoplasm of the apoptotic cells is observed (arrowheads). (C,G) In granulosa cell explants cultured under 24h-SF-H supplementation, gap junctions stain more intensely and are larger compared with 24h-SF. (D,H) In granulosa cell explants cultured under 24h-SF-Ca supplementation, a punctate pattern of many small gap junctions is observed. Fragmentation of gap junctions is apparent. (J) Apoptotic granulosa cells are organized in clustered or streaks. Bars = 10 μm.

Figure 3.

(A) Cx43 immunofluorescence intensity as determined from the average intensity in confocal laser scanning micrographs. Immunofluorescence intensity was lower in 24h-SF cultured granulosa cell explants compared with freshly isolated granulosa cell sheets (Fresh), and was again higher in the 24h-SF-H and 24h-SF-Ca conditions. Cx43 immunofluorescence is expressed relative to a 100% control level of freshly isolated granulosa cell sheets. ∗, # p<0.001 compared with all other groups; n = 36 in one animal. All fields investigated contained equal numbers of cells. (B) Immunoblot probed with mouse monoclonal anti-Cx43 antibody, showing the expression level of Cx43 in granulosa cells explants. Lane 1, freshly isolated; Lane 2, 24-SF; Lane 3, 24-SF-H; Lane 4, 24-SF-Ca; Lane 5, positive control with rat cerebrum lysates. This immunoblot is representative of three similar experiments. (C) Inverse relation of Cx43 immunoreactivity and apoptotic indexes. The Cx43 immmunoreactivity expressed in abcisssa are average values calculated from the experiments presented in A and B.

Level of Cx43 Expression as Studied by Western Blotting

Granulosa cell explants, both cultured and uncultured, express a 43-kD protein that reacts with Cx43-specific antibodies on Western blots. Immunoblots of whole cell lysates showed that 24h-SF granulosa cells explants contained only 39% of what is expressed in freshly isolated granulosa cells sheets, while the level of Cx43 expression recovered to 74% and 76% under the 24h-SF-H and 24h-SF-Ca conditions, respectively (Figure 3B), which is in accordance with the data obtained by ICC analysis. Combination of the quantitative data from ICC analysis and Western blotting showed that Cx43 expression was inversely related to the AIs (Figure 3C).

Electron Microscopy

Electron microscopic analysis of granulosa cells demonstrated rare gap junctions in freshly isolated granulosa cells despite the positive Cx43 immunoreactivity described above, and showed huge gap junctions in 24h-SF and 24h-SF-H conditions (Figure 4). In 24h-SF-Ca conditions, many short gap junctions were observed in accordance with the ICC punctate pattern (Figure 4). Internalization of gap junctions did not occur, as evidenced by the absence of annular junctions.

Figure 4.

Electron microscopic micrographs. (A) Granulosa cell ex-plants cultured in 24h-SF condition. Note a huge gap junction (arrows). (B,C) Granulosa cell explants cultured in 24h-SF-Ca supplemented condition. Fragmented dot-like gap junctions (arrows). (B) An overview of C. Bars: A,C = 0.5 μm; B = 1 μm.

Functional Coupling as Studied by FRAP

The level of gap junctional coupling in granulosa cell explants cultured in 24h-SF conditions was significantly higher compared with the freshly isolated granulosa cell sheets (Figure 5). In 24h-SF-H treated granulosa cell explants, the level of gap junctional coupling was comparable to the freshly isolated conditions, while granulosa cell explants kept under 24h-SF-Ca displayed the highest level of coupling (Figure 5).

Effect of Gap Junction Blockade Experiments on Apoptotic Indexes

To investigate the effect of gap junction blocking on cell survival, granulosa cell explants were incubated for 24 hr in serum-free condition with increasing doses of AGA (25 μM to 250 μM range) and AIs were evaluated by DAPI staining. These results showed that the AI was dose-dependently decreased by AGA, with a half-maximal effect occurring at ≈40 μM (Figure 6). FRAP experiments showed that 24 hr exposure of the granulosa cell explants to AGA caused a drastic reduction of gap junctional coupling, with 250 μM AGA decreasing the fluorescence recovery from a control level of 45.8 ± 2.6% to 3.2 ± 0.6% (n=8 in one animal; p<0.00001).

Discussion

The purpose of the present study was to investigate the role of cell coupling and level of connexin43 expression in apoptosis and survival of granulosa cell ex-plants. We demonstrated that apoptosis induction in granulosa cell explants is accompanied by an increased level of gap junctional coupling, that apoptosis was dose-dependently inhibited by the gap junction blocker AGA, and that apoptosis induction is accompanied by a decreased level of Cx43 expression, which is regained by the two applied survival protocols.

Figure 5.

Gap junctional coupling as studied with FRAP. Gap junctional coupling was more prominent (larger extent of fluorescence recovery) in 24h-SF conditions compared with freshly isolated conditions (Fresh). Under 24h-SF-H conditions, gap junctional coupling was no different from the freshly isolated condition, whereas the 24h-SF-Ca condition was associated with a very large coupling degree. ∗p<0.05, ∗∗p<0.01 compared with the fresh condition; n = 25 in at least three different animals.

Figure 6.

Dose-response relation between inhibition of gap junctional coupling with increasing doses of AGA and the resulting effect on AI. AGA dose-dependently inhibited apoptosis, with half-maximal inhibition occurring with concentrations in the order of 40 μM. ∗p<0.05 compared with control (con).

Granulosa cell explants provide a model system closely mimicking the apoptotic process that occurs in vivo during spontaneous follicular atresia (D'Herde and Leybaert 1997). Contributing factors to explain the relatively low apoptotic rates in 24h-SF granulosa cell explants might be the presence of native extracellular matrix, i.e., the vitelline and basement membranes, which are still present in the used model system and which are known to promote survival (Amsterdam et al. 1989; Boudreau et al. 1995). Second, as reviewed by Johnson (2000), death-suppressing members of the Bcl-2 family (Bcl-xLong, Bcl-2) are expressed in granulosa cells from preovulatory follicles. Another contributing factor is the expression of IAP-1, an inhibitor of apoptosis protein found in preovulatory hen granulosa cells (Johnson et al. 1998).

The data of the present study demonstrated that, in freshly isolated granulosa cells, Cx43 immunolabeling was observed all around the cells, even in those regions of the plasma membrane where visible contact with any apposing plasma membrane of neighboring cells was missing. In the absence of any apposing cells, immunolabeling in the non-junctional plasma membrane regions is assumed to represent hemichannels of Cx43 (Musil and Goodenough 1991; Zampighi et al. 1999; Quist et al. 2000). Quantification of the Cx43 immunoreactivity (as revealed by ICC and Western blotting) in freshly isolated granulosa cell sheets showed a higher amount of Cx43 protein compared with 24h-SF or 24h-SF-H supplemented cultures in which at EM level long gap junctions between neighboring cells are easily detected. Despite the detected Cx43 immunolabeling and coupling of the cells as assessed by FRAP, extremely rare gap junctions were detected by EM analysis in these freshly isolated granulosa cells. This discrepancy between ultrastructural detection of gap junctions and ICC detection of their major component Cx43 might be explained by the fact that immunoreactivity is not confined only to gap junctions but also reveals Cx43 present in the cytoplasm or organized as connexin hemichannels. Moreover, one should consider the fact that clustering of gap junctions into plaques is the primary basis for their ultrastructural identification (Goodenough and Revel 1970; McNutt and Weinstein 1970).

In 24h-SF cultured granulosa cell explants, the apoptosis-inducing condition, well-organized gap junctions were observed (as revealed by ICC and EM), providing a better coupling of the granulosa cells than in freshly isolated granulosa cells as measured by FRAP analysis. However, a lower immunoreactive signal was detected in these 24h-SF granulosa cells (as revealed by quantitative analysis of both the confocal images and immunoblots) compared with freshly isolated granulosa cells. These apparently inconsistent findings underscore the fact that the abundance of Cx43 does not necessarily correlate with the amount of intracellular communication (Lau et al. 1992; Kurata and Lau 1994; Laing et al. 1997; Sia et al. 1999).

In agreement with the data from the present study, Wilson et al. (2000) found an average increase of gap junctional coupling during initiation of apoptosis in serum-deprived (24-hr) cultures of a normal diploid epithelial cell line (WB-F344), assessed by the scrape load/dye transfer method. In the same study, individual cells were analyzed by the FRAP assay to compare nuclear morphology to differences in dye transfer during serum deprivation. The authors demonstrated that most apoptotic bodies did not communicate with neighboring cells. This is in line with the present ICC findings revealing that granulosa cells with clear apoptotic nuclear morphology do not show Cx43 immunolabeling at their plasma membrane. Kalvelyte et al. (2003) reported that expression of Cx43 in HeLa-transfected cells accelerates the transition of cells from an early phase of apoptosis to the late phase.

To verify whether cell coupling is indeed a means of propagating a cell death message, experiments using the gap junctional intercellular communication inhibitor AGA were carried out. This compound was shown to block cell coupling (Davidson et al. 1986), presumably by disassembling gap junctional channels and affecting their dephosphorylation (Guan et al. 1996). In the present model system, gap junctional coupling was significantly inhibited after long-term AGA exposure. Moreover, it was demonstrated that, by blocking gap junctional coupling with AGA, AIs were dose-dependently decreased (Figure 6). In agreement with the present data, Krutovskikh et al. (2002) showed that in BC31 cells (a rat bladder carcinoma cell line) cell coupling and apoptosis were significantly inhibited by treatment with AGA. In addition, Lin et al. (1998) reported that gap junctions achieved by transfection of Cx43 could mediate the propagation of a death signal between dying and healthy glial cells in a co-culture system.

A further argument in support of a role of cell coupling in the propagation of a death signal among granulosa cells comes from DAPI-stained granulosa explants in the 24h-SF condition (Figure 2J), which demonstrates that granulosa cells did not die randomly but, rather, in tracks of closely associated cells. This finding supports the observation described by Cotrina et al. (1998) and Krutovskikh et al. (2002) that communicating cells die by clusters. Although apoptosis in granulosa cell explants is associated with increased cell coupling (as discussed above), it is important to note that increased cell coupling does not necessarily induce apoptosis per se, as illustrated in the Ca²⁺-rescue protocol. Therefore, our findings and those of others (Lin et al. 1998; Sai et al. 2001; Krutovskikh et al. 2002; Kalvelyte et al. 2003) are consistent with the hypothesis that gap junctional intercellular communication is indeed needed for cell-cell transmission of a death signal and thus may contribute to the regulation of the apoptotic process in diverse model systems.

It is known that gap junctions are permeable to several second messengers, such as inositol-1,4,5-triphosphate, ATP, cAMP, and calcium.

In a rat bladder carcinoma line, it has been demonstrated that a reduction of gap junction permeability with oleamide, which appears not to affect the passage of Ca²⁺ ions, did not abrogate coordinated cell death by clusters, suggesting that Ca²⁺ ions are involved as the signal propagating cell death (Krutovskikh et al. 2002).

Interestingly, Cx43 immunoreactivity (as studied by ICC and immunoblotting) was inversely correlated with apoptosis (Figure 3C), suggesting that Cx43 expression plays a role in the survival process. Sasson and Amsterdam (2002) reported that apoptosis induction by LH and forskolin was accompanied by increased expression of Cx43 in human luteinized granulosa cells, a discrepancy that might be related to the use of different cell models and apoptosis-inducing stimuli. Several reports point to the fact that Cx43 plays a role in promoting survival in response to extracellular cues independently of gap junctional intercellular communication (Plotkin and Bellido 2001; Plotkin et al. 2002). Huang et al. (2001) demonstrated that regulation of apoptosis by Cx43 in human glioblastoma cells is mediated by downregulation of the apoptosis inhibitor protein Bcl-2 and hence is not linked to gap junction channel function. Recently, Lin et al. (2003) demonstrated that forced expression of Cx43 as well as two other members of the connexin family (Cx32 and Cx40) increased the resistance of astrocytes to injury and that the anti-death activity of connexin proteins was independent of the functional status of gap junctions.

In conclusion, the present study suggests that gap junctions mediate the propagation of a cell death message, while independently Cx43 expression is needed for survival. Future studies will focus on the experimental modulation of Cx43 expression and its effects on the major pro- and anti-apoptotic signaling pathways.

Footnotes

Acknowledgements

Supported by Ghent University (GOA grant no. 12050502 to KD) and the Fund for Scientific Research Flanders, Belgium (FWO grant nos. 3G023599, 3G001201, and G.0335.03 to LL).

We are very grateful to Barbara De Bondt, Dominique Jacobus, and Hubert Stevens for excellent technical assistance.

References

Adashi

(1992) The potential relevance of cytokines to ovarian physiology. J Steroid Biochem Mol Biol 43:439–444

Adashi

Resnick

Hurwitz

Ricciarelli

Hernandez

Roberts

Leroith

. (1991) Insulin-like growth factors: the ovarian connection. Hum Reprod 6:1213–1219

Amsterdam

Rotmensch

Furman

Venter

Vlodavsky

(1989) Synergistic effect of human chorionic gonadotropin and extracellular matrix on in vitro differentiation of human granulosa cells: progesterone production and gap junction formation. Endocrinology 124:1956–1964

Beyer

(1990) Molecular cloning and developmental expression of two chick embryo gap junction proteins. J Biol Chem 265:14439–14443

Boudreau

Sympson

Werb

Bissell

(1995) Suppression of ICE and apoptosis in mammary epithelial cells by extracellular matrix. Science 267:891–893

Bradford

(1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

Braet

Vandamme

Martin

Evans

Leybaert

(2003) Photoliberating inositol-1,4,5-trisphosphate triggers ATP release that is blocked by the connexin mimetic peptide gap 26. Cell Calcium 33:37–48

Bruzzone

White

Goodenough

(1996) The cellular internet: on-line with connexins. Bioessays 18:709–718

Cotrina

Kang

Lin

JHC

Bueno

Hansen

Liu

. (1998) Astrocytic gap junctions remain open during ischemic conditions. J Neurosci 18:2520–2537

10.

Davidson

Baumgarten

Harley

(1986) Reversible inhibition of intercellular junctional communication by glycyrrhetinic acid. Biochem Biophys Res Commun 134:29–36

11.

Dekel

(1987) Interaction between the oocyte and the granulosa cells in the preovulatory follcile. In Armstrong

Freisen

Leung

PCK

Moger

Ruf

Endocrinology and Physiology of Reproduction. New York, Plenum Publishing, 197–209

12.

D'Herde

De Prest

Mussche

Schotte

Beyaert

Coster

Roels

(2000) Ultrastructural localization of cytochrome c in apoptosis demonstrates mitochondrial heterogeneity. Cell Death Differ 7:331–337

13.

D'Herde

Leybaert

(1997) Intracellular free calcium related to apoptotic cell death in quail granulosa cell sheets kept in serum-free culture. Cell Death Differ 4:59–65

14.

Di Virgilio

Steinberg

Swanson

Silverstein

(1988) Fura-2 secretion and sequestration in macrophages. A blocker of organic anion transport reveals that these processes occur via a membrane transport system for organic anions. J Immunol 140:915–920

15.

Farioli-Vecchioli

Raes

Espeel

Roels

D'Herde

(2000) Inverse expression of peroxisomes and connexin-43 in the granulosa cells of the quail follicle. J Histochem Cytochem 48:167–178

16.

Gilbert

Evans

Perry

Davidson

(1977) A method for separating the granulosa cells, the basal lamina and the theca of the preovulatory ovarian follicle of the domestic fowl (Gallus domesticus). J Reprod Fertil 50:179–181

17.

Gittens

Mhawi

Lidington

Ouellette

Kidder

(2003) Functional analysis of gap junctions in ovarian granulosa cells: distinct role for connexin43 in early stages of folliculogenesis. Am J Physiol 284:C880–887

18.

Goodenough

Revel

(1970) A fine structural analysis of intercellular junctions in the mouse liver. J Cell Biol 45:272–290

19.

Grazul-Bilska

Reynolds

Redmer

(1997) Gap junctions in the ovaries. Biol Reprod 57:947–957

20.

Guan

Wilson

Schlender

Ruch

(1996) Gap-junction disassembly and connexin 43 dephosphorylation induced by 18 beta-glycyrrhetinic acid. Mol Carcinog 16:157–164

21.

Huang

Hossain

Huang

Gano

Fan

Boynton

(2001) Connexin 43 (cx43) enhances chemotherapy-induced apoptosis in human glioblastoma cells. Int J Cancer 92:130–138

22.

Jiang

Cheung

Wang

Tsang

(2003) Regulation of cell death and cell survival gene expression during ovarian follicular development and atresia. Front Biosci 8:d222–237

23.

Johnson

(2000) Granulosa cell apoptosis: conservation of cell signaling in an avian ovarian model system. Biol Signals Recept 9:96–101

24.

Johnson

Bridgham

Digby

Lowenthal

(1998) Expression of the inhibitor of T-cell apoptosis (ita) gene in hen ovarian follicles during development. Biol Reprod. 58:414–420

25.

Kalvelyte

Imbrasaite

Bukauskiene

Verselis

Bukauskas

(2003) Connexins and apoptotic transformation. Biochem Pharmacol 66:1661–1672

26.

Kidder

Mhawi

(2002) Gap junctions and ovarian folliculogenesis. Reproduction 123:613–620

27.

Krutovskikh

Piccoli

Yamasaki

Yamasali

(2002) Gap junction intercellular communication propagates cell death in cancerous cells. Oncogene 21:1989–1999

28.

Krysko

Roels

Leybaert

D'Herde

(2001) Mitochondrial transmembrane potential changes support the concept of mitochondrial heterogeneity during apoptosis. J Histochem Cytochem 49:1277–1284

29.

Kumar

Gilula

(1996) The gap junction communication channel. Cell 84:381–388

30.

Kurata

Lau

(1994) p130gag-fps disrupts gap junctional communication and induces phosphorylation of connexin43 in a manner similar to that of pp60v-src. Oncogene 9:329–335

31.

Laing

Tadros

Westphale

Beyer

(1997) Degradation of connexin43 gap junctions involves both the proteasome and the lysosome. Exp Cell Res 236:482–492

32.

Lau

Kanemitsu

Kurata

Danesh

Boynton

(1992) Epidermal growth factor disrupts gap-junctional communication and induces phosphorylation of connexin43 on serine. Mol Biol Cell 3:865–874

33.

Lin

Weigel

Cotrina

Liu

Bueno

Hansen

. (1998) Gap-junction-mediated propagation and amplification of cell injury. Nature Neurosci 1:494–500

34.

Lin

JHC

Yang

Liu

Takano

Wang

Gao

Willecke

. (2003) Connexin mediates gap junction-independent resistance to cellular injury. J Neurosci 23:430–441

35.

McNutt

Weinstein

(1970) The ultrastructure of the nexus. A correlated thin-section and freeze-cleave study. J Cell Biol 47:666–688

36.

Moorby

Patel

(2001) Dual functions for connexins: Cx43 regulates growth independently of gap junction formation. Exp Cell Res 271:238–248

37.

Munari-Silem

Rousset

(1996) Gap junction-mediated cell-to-cell communication in endocrine glands-molecular and functional aspects: a review. Eur J Endocrinol 135:251–264

38.

Musil

Beyer

Goodenough

(1990) Expression of the gap junctional protein connexin-43 in embryonic chick lens: molecular cloning, ultrastructural localization and post-translational phosphorylation. J Membr Biol 116:163–175

39.

Musil

Goodenough

(1991) Biochemical analysis of connexin43 intracellular transport, phosphorylation, and assembly into gap junctional plaques. J Cell Biol 115:1357–1374

40.

Mussche

Leybaert

D'Herde

(2000) First and second messenger role of calcium. Survival versus apoptosis in serum-free cultured granulosa explants. Ann NY Acad Sci 926:101–115

41.

Onagbesan

Peddie

(1988) Induction of ovulation and oviposition in female quail with luteinizing hormone, luteinizing hormone releasing hormone, or progesterone. Gen Comp Endocrinol 71:124–131

42.

Plotkin

Bellido

(2001) Bisphosphonate-induced, hemichannel-mediated, anti-apoptosis through the Src/ERK pathway: a gap junction-independent action of connexin43. Cell Commun Adhes 8:377–382

43.

Plotkin

Manolagas

Bellido

(2002) Transduction of cell survival signals by connexin-43 hemichannels. J Biol Chem 277:8648–8657

44.

Quist

Rhee

Lin

Lal

(2000) Physiological role of gap-junctional hemichannels. Extracellular calcium-dependent isosmotic volume regulation. J Cell Biol 148:1063–1074

45.

Rosenfeld

Wagner

Roberts

Lubahn

(2001) Intra-ovarian actions of oestrogen. Reproduction 122:215–226

46.

Sai

Kang

Hirose

Hasegawa

Trosko

Inoue

(2001) Inhibition of apoptosis by pentachlorophenol in v-myc-transfected rat liver epithelial cell: relation to down-regulation of gap junctional intercellular communication. Cancer Lett 173:163–174

47.

Sanderson

Parker

(2003) Video-rate confocal microscopy. In Marriott

Parker

Biophotonics. Methods in Enzymology. Vol 360. San Diego, Academic Press, 447–481

48.

Sasson

Amsterdam

(2002) Stimulation of apoptosis in human granulosa cells from in vitro fertilization patients and its prevention by dexamethasone: involvement of cell contact and bcl-2 expression. J Clin Endocrinol Metab 87:3441–3451

49.

Sia

Woodward

Turner

Laird

(1999) Quiescent mammary epithelial cells have reduced connexin43 maintain a high level of gap junction intercellular communication. Dev Genet 24:111–122

50.

Sommersberg

Bulling

Salzer

Frohlich

Garfield

Amsterdam

Mayerhofer

(2000) Gap junction communication and connexin 43 gene expression in a rat granulosa cell line: regulation by follicle-stimulating hormone. Biol Reprod 63:1661–1668

51.

Tilly

(1997) Apoptosis and the ovary: a fashionable trend or food for thought? Fertil Steril 67:226–228

52.

Trosko

Ruch

(1998) Cell-cell communication in carcinogenesis. Front Biosci 3:D208–236

53.

Wilson

Trosko

(2000) Cell population dynamics (apoptosis, mitosis, and cell-cell communication) during disruption of homeostasis. Exp Cell Res 254:257–268

54.

Zampighi

Loo

Kreman

Eskandari

Wright

(1999) Functional and morphological correlates of connexin50 expressed in Xenopus laevis oocytes. J Gen Physiol 113:507–524

Gap Junctional Communication and Connexin43 Expression in Relation to Apoptotic Cell Death and Survival of Granulosa Cells

Abstract

Keywords

Materials and Methods

Isolation and Culture of Granulosa Cell Sheets

Quantification of Apoptosis by DAPI Staining

Antibodies

Immunocytochemistry of Connexin43

Quantification of Cx43 Immunofluorescence

Protein Extraction and Western Blotting Analysis of Cx43 Expression

Transmission Electron Microscopy

Fluorescence Recovery After Photobleaching (FRAP)

Gap Junction Blocking by 18 α-Glycyrrhetinic Acid

Statistical Analysis

Results

Apoptosis and Cx43 ICC

Level of Cx43 Expression as Studied by Western Blotting

Electron Microscopy

Functional Coupling as Studied by FRAP

Effect of Gap Junction Blockade Experiments on Apoptotic Indexes

Discussion

Footnotes

Acknowledgements

References