Abstract
— Seven phosphatase inhibitors were used to investigate the possible importance of phosphatases in the regulation of gap junctional intercellular communication (GJIC). Only the tyrosine phosphatase inhibitor, pervanadate, was found to inhibit GJIC and to alter the band pattern of connexin43 (Cx43) in Western blots. Only the unphosphorylated band of Cx43 was seen after alkaline phosphatase treatment of pervanadate-exposed cells. Application of a tyrosine-specific phosphatase and a phosphotyrosine antibody clearly indicated that pervanadate induced tyrosine phosphorylation in Cx43.
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