Protective Effect of Chikusetsu saponin IVa on Hydrogen Peroxide Induced Injury of IPEC-J2 Cells

Abstract

Objectives: Chikusetsu saponin IVa (CHS-IVa) is a saponin compound widely found in herbs such as Panax japonicus C.A.Mey, which has various functions. The purpose of this study was to investigate the protective effect of CHS-IVa on oxidative stress and inflammation in porcine intestinal epithelial cells (IPEC-J2) under the action of hydrogen peroxide (H₂O₂). Methods: Cell viability was detected by MTT method. The cell scratch experiment was used to test the repair ability of CHS-IVa on cells. The levels of lactate dehydrogenase (LDH), catalase (CAT), superoxide dismutase (SOD), glutathione (GSH), thioredoxin reductase (TrxR), and malondialdehyde (MDA) were tested using the relevant kits. The expression levels of TNF-α, NF-κB, IL-6, IL-1α, and P65 were detected by qPCR. Results: MTT results showed that CHS-IVa at 50 to 100 μg/mL could enhance the activity of IPEC-J2 cells within 24 hours. CHS-IVa at 80 and 100 μg/mL could promote damage repair and effectively inhibit the release of LDH production in IPEC-J2 cells after exposure to H₂O₂. Additionally, it had a restorative effect on the loss of TrxR, SOD, GSH, and CAT. At the same time, the treatment of CHS-IVa significantly decreased the mRNA expression of NF-κB, IL-6, and P65 after H₂O₂ treatment. Conclusion: These findings indicate that a specific concentration of CHS-IVa has a protective effect on IPEC during oxidative stress, thereby enhancing their ability to repair damage.

Keywords

IPEC-J2 antioxidant damage protection

Introduction

Panax japonicus (PJ) is the Araliaceae Panax family, and it is found in the wild in southwestern China and Japan.^1,2 The pharmacological components of PJ mainly include bioactive components such as saponins, organic acids, and polysaccharides, among which saponins are the most abundant.³ Saponin is a class of triterpenoids or heliosteranes.⁴ Chikusetsu Saponin IVa (CHS-IVa) is one of the major saponins in PJ.⁵ The parent nucleus of CHS-IVa is a ziasterane-type pentacyclic triterpenoid compound with a molecular weight of 794.97. The chemical formula is C₄₂H₆₆O₁₄, and its chemical structure is shown in Figure 1. Studies have shown that CHS-IVa and its derivatives have various pharmacological activities, including anti-inflammatory,⁶ and antioxidant,⁷ as well as liver protection.⁸ They can also play a role in hypoglycemic effects,⁹ regulating metabolism,¹⁰ and anti-virus activity.¹¹ Studies have shown that CHS-IVa has a positive effect on cardiomyocyte injury in rats, mainly by scavenging the excessive production and accumulation of reactive oxygen species (ROS) caused by high glucose to alleviate the injury.¹² Wang et al found that CHS-IVa can effectively inhibit the production of inflammatory cytokines by human monocytic-leukemia cells (THP-1) macrophages.⁶

Figure 1.

The chemical structure of CHS-IVa.¹⁰

Intestinal epithelial cells (IPEC) are a component of the intestinal epithelium that can separate the intestinal mucosa from the intestinal lumen environment.¹³ They restrict the entry of toxins and pathogens, among other things, into mucosal tissues.¹⁴ Oxidative stress can cause damage to IPEC, leading to tight junction damage and systemic endogenous stress syndrome, and disrupting the self-renewal process of the intestine,^15,16 leading to the development of intestinal degenerative diseases such as inflammation and cancer.¹⁷ When cells are stimulated, the intracellular enzyme antioxidant system, including superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT), glutathione (GSH), and thioredoxin reductase (TrxR), undergoes changes to maintain good in vivo balance. When cells are stimulated, the intracellular enzyme antioxidant system, including SOD,¹⁸ MDA, CAT, GSH,¹⁹ and TrxR, undergo changes to maintain a fine homeostasis. Many natural products have anti-inflammatory, antioxidant, and other protective effects. Demircigil et al found that the main bioactive components in black cumin essential oil, such as thymoquinone, can exert antioxidant activity by increasing the content of CAT, GSH, and SOD.²⁰ The study by Ghasemi et al showed that the methanol extract of Teucrium hyrcanicum L. protects mouse embryonic fibroblast cells (NIH/3T3) lines from H₂O₂-induced oxidative stress by decreasing MDA formation and inhibiting GSH oxidation.²¹ Many studies have shown that the saponins from PJ have a protective effect on the gut. Dun et al have shown that Saponins from PJ can inhibit inflammation, regulate intestinal epithelial tight junction damage and down-regulate mitogen-activated protein kinase (MAPK) and nuclear factor kappa-B (NF-κB) signaling pathway phosphorylation in aging rats.²² PJ total saponins have altered the composition of the intestinal flora in experimental autoimmune encephalomyelitis mice and restored the ratio of Firmicutes to Bacteroides.²³ CHS-IVa is also the main component in PJ total saponins.²⁴ Therefore, this study took this as a starting point to explore whether CHS-IVa has a protective effect on porcine intestinal epithelial cells (IPEC-J2).

Materials and Methods

Experimental Reagents

CHS-IVa (purity 99.99%) was purchased from Chengdu Must Biotechnology Co., Ltd (Chengdu, China); 30% H₂O₂ (excellent pure) and absolute ethanol (analytical pure) were purchased from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China); fetal bovine serum and DMEM high-sugar medium were purchased from Gibco (New York, USA); penicillin-streptomycin was purchased from Procell Life Science &Technology Co., Ltd (Wuhan, China); MTT solution was purchased from WellBio (Changsha, China); Formazan was purchased from Conway Century (Jiangsu, China); LDH Cytotoxicity Assay Kit was purchased from Solabel (Shanghai, China); CAT, SOD, GSH, TrxR, and MDA were purchased from Suzhou Keming Biotechnology Co., Ltd (Suzhou, China); Transzol up reagent was purchased from Quanshijin (Beijing, China); Reverse transcription kits were purchased from TaKaRa (Beijing, China); qRT-PCR kit was purchased from Vazyme (Nanjing, China).

Drug Configuration

900 μL of PBS solution was mixed with 100 μL of a 30% H₂O₂ solution in a centrifuge tube and filtered through a 0.22-μm sterile needle filter to obtain a 1 × 10⁶ μM H₂O₂ stock solution. 2 × 10⁵ μg/mL CHS-IVa stock solution was prepared by 0.5 mL of DMSO and 0.1000 g of CHS-IVa powder. Each concentration of reagent in the experiment was obtained by diluting the stock solution according to the corresponding method.

Cell and Cell Culture

IPEC-J2 was purchased from Guangzhou JENNIO Bio-Technology Co., Ltd (Guangzhou, China). The cells were cultured in a thermostatic incubator at 37 °C and 5% CO₂, and the complete medium was configured with a ratio of 89% DMEM, 1% penicillin–streptomycin, and 10% fetal calf serum.

Cell Viability Assay

In order to select the optimal H₂O₂ concentration, growing logarithmic IPEC cells were seeded in 96-well plates. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) was used to detect the viability of IPEC-J2 cells. According to the experimental group, the corresponding H₂O₂ dilution (0, 250, 500, 750, and 1000 μM) was added to IPEC cells for 2 hours or 4 hours. Then, discarding the culture medium, 110 μL of MTT solution was added to the wells. After incubation at 37 °C for 2 hours, 150 μL of formazan replaced MTT and was left to stand for 10 minutes. Then we determined the OD of each well at a wavelength of 490 nm and calculated cell viability according to Cell activity = OD₄₉₀ (treatment-blank)/OD₄₉₀ (control-blank) × 100%.²⁵ To select the most suitable CHS-IVa concentration, growing logarithmic IPEC cells were seeded in 96-well plates. CHS-IVa dilution (0, 50, 100, 200, and 400 μg/mL) and complete medium were applied to IPEC cells for 9, 24, and 48 hours. Similarly, CHS-IVa dilution (0, 60, 80, and 100 μg/mL) and complete medium were applied to IPEC cells for 3 and 6 hours. The MTT method was used to detect cell viability.

To investigate the effect of CHS-IVa on cell viability of H₂O₂-damaged IPEC-J2 cells, logarithmic growth phase cells were seeded in 96-well culture plates. First, 1000 μM H₂O₂ was added to the air pretreatment for 2 hours. Then 80 and 100 μg/mL of CHS-IVa was respectively incubated for 6 hours. Afterward, the cell activity was tested with the MTT assay.

Cell Scratch Experiment

IPEC-J2 in logarithmic growth phase was seeded in 6-well plates. After placing the 6-well plate lid in the center of each well, the tip of the 10 μL pipette was kept perpendicular to the 6-well plate lid and streaked. After scribing, the medium supernatant was removed after streaking was complete and PBS was added to remove residual cell debris.²⁶ CHS-IVa solutions at concentrations of 0, 80, 100, and 200 μg/mL diluted with DMEM medium were sequentially added to the wells. Image information was collected after 0, 8, 12, and 24 hours of drug action. Damage rate = scratch area at time t/initial scratch area.²⁷

Measurement of LDH, CAT, SOD, GSH, TrxR, and MDA

Commercial reagent kits were used to detect the levels of LDH, CAT, SOD, GSH, TrxR and MDA, and follow the steps provided by the manufacturer for the experiment.

qPCR

The total RNA of IPEC-J2 was extracted using TransZol Up reagent. cDNA was extracted using reverse transcription kits. The primer sequences of qPCR gene are listed in the table below (Table 1), and according to the qRT-PCR kit, the reaction reagents were fully mixed with cDNA,²⁸ and the qPCR procedure was set: 94 °C, 30 s; 94 °C, 5 s, 60 °C, 30 s, 40 cycles; extension at 65 °C.

Table 1.

Oligonucleotide Primers Used for Real-Time PCR.

Gene		Primer Sequence (5′–3′)	GenBank No.
GAPDH	F	ACAGGGTGGTGGACCTCATG	XM_020191114.1
GAPDH	R	GGGTCTGGGATGGAAACTGG	XM_020191114.1
IL-6	F	AGCAAGGAGGTACTGGCAGA	XM_021082686.1
IL-6	R	TGGAG1TGTCGGTGTAGATGC	XM_021082686.1
TNF-α	F	GACTCAGATCATCGTCTC	XM_013977858.2
TNF-α	R	GGAGTAGATGAGGTACAG	XM_013977858.2
P65	F	CAACCCCTTCCAAGTTCCCATAG	NM_001114281.1
P65	R	CCGAGTTCCGATTCACCCG	NM_001114281.1
NF-κB	F	CTCGCACAAGGAGACATGAA	NM_001048232.1
NF-κB	R	ACTCAGCCGGAAGGCATTAT	NM_001048232.1
IL-1α	F	CCAATGACACAGAAGAAG	XM_013987857.2
IL-1α	R	CCAGGTTATTTAGCACAG	XM_013987857.2

Statistics and Analysis

The analysis of experiment results in this paper was expressed as “mean ± standard deviation,” and each group of experiments was repeated at least three times. All data were statistically analyzed by GraphPad Prism 8 software. The * sign in the data plot indicates a statistically significant difference between the two groups, P < .05. When the difference is not significant, the P > .05 cell morphology picture was collected using Motic Images Plus 3 software and then Image J was used to calculate the required data.

Results

Different Concentrations of H₂O₂ Reduced the Viability of IPEC-J2 Cells

Due to its strong oxidizing properties, H₂O₂ is often used to establish an oxidative damage model.²⁹ The viability of IPEC-J2 cells was detected after 2 hours of different concentrations of H₂O₂. Cell viability was significantly reduced after 2 hours treatment with 750 μM and 1000 μM H₂O₂ compared to the control group (P < .05) (Figure 2a). After 4 hours of treatment, the cell activity of the H₂O₂ group at a concentration of 1000 μM decreased significantly (P < .05) (Figure 2b). 1000 μM H₂O₂ can reduce cell viability at 2 hours, which was chosen for subsequent experiments.

Figure 2.

Cell viability of IPEC-J2 cells at different times treated with different concentrations of H₂O₂. (a) 2 hours; (b) 4 hours. Data are presented as mean ± SD. *P < .05 was statistically significant compared to the control group.

Different Concentrations of CHS-IVa Regulated the Viability of IPEC-J2 Cells

Firstly, the viability of IPEC-J2 cells treated with different concentrations of CHS-IVa for 9, 24, and 48 hours was detected. When 50, 100, and 200 μg/mL CHS-IVa were treated for 9 hours, the viability of IPEC-J2 cells was increased significantly (P < .05) (Figure 3a). At 24 hours of treatment, IPEC-J2 cell viability still showed a significant upward trend in the 50 and 100 μg/mL groups (P < .05), but there was no significant change in cell viability (P > .05) at 24 hours in CHS-IVa at 200 μg/mL (Figure 3b). By 48 hours, the cell viability of the CHS-IVa group at a concentration of 200 μg/mL decreased significantly (P < .05), and the cell viability was no longer significantly increased in the 100 μg/mL group (Figure 3c). It shows that 100 μg/mL CHS-IVa has no toxic effect on cells within 24 hours. Then, the viability of different concentrations of CHS-IVa on IPEC-J2 cells was detected for 3 and 6 hours (Figure 3d and e), and it was found that the cell viability was significantly increased after 60, 80, and 100 μg/mL CHS-IVa acted on IPEC-J2 for 3 and 6 hours (P < .05). In order to better exert the pharmacological activity of CHS-IVa, we chose 80 and 100 μg/mL CHS-IVa to act on IPEC-J2 cells for 6 hours in subsequent experiments.

Figure 3.

Cell viability of IPEC-J2 cells at different times treated with different concentrations of CHS-IVa. (a) 9 hours; (b) 24 hours; (c) 48 hours; (d) 3 hours; (e) 6 hours. Data are presented as mean ± SD. *P < .05 was statistically significant compared to the control group.

Different Concentrations of CHS-IVa Repaired the Scratch Damage of IPEC-J2 Cells

After CHS-IVa treatment of 80 and 100 μg/mL for 0 to 24 hours, the damage rate at the cell scribing gradually decreased (Figure 4a and b). The restoration effect of CHS-IVa at 100 μg/mL was obvious. The repair effect of CHS-IVa at 200 μg/mL on cells was slight after 8 and 12 hours, but the repair rate of cells significantly decreased after 24 hours of treatment.

Figure 4.

Effects of different concentrations of CHS-IVa on the repair of IPEC-J2 cells after injured. (a) Results of scratch test; (b) Damage rate (%). The scales are 100 μm. Data are presented as mean ± SD. *P < .05 was statistically significant compared to the control group.

CHS-IVa Repaired the Damage of IPEC-J2 Cells after H₂O₂ Treatment

Compared with the group treated with 1000 μM H₂O₂ for 2 hours, the activity of IPEC-J2 cells significantly increased after 6 hours in the 80 and 100 μg/mL CHS-IVa groups (P < .05) (Figure 5a). The results showed that 80 and 100 μg/mL had a certain protective effect on cells damaged by H₂O₂. LDH exists inside the cell and is released outside the cell when it is damaged or dies.³⁰ We detected changes in LDH viability in the culture supernatant of IPEC-J2 cells injured by 1000 μM H₂O₂ under different concentrations of CHS-IVa. LDH activity was significantly decreased in the 80 and 100 μg/mL CHS-IVa treatment groups compared with the 1000 μM H₂O₂ single treated group (Figure 5b).

Figure 5.

Effects of different concentrations of CHS-IVa on cell viability (a) and LDH activity (b) of IPEC-J2 cells treated with H₂O₂. Data are presented as mean ± SD. *P < .05 was statistically significant compared to the control group.

Regulation of CHS-IVa on the Changes of MDA Content and Antioxidant Enzyme Activity in IPEC-J2 Cells Treated With H₂O₂

Next, we examined the effect of different concentrations of CHS-IVa on the activity changes of antioxidant enzymes (including CAT, SOD, GSH, TrxR) in the culture supernatant of IPEC-J2 cells after the treatment of 1000 μM H₂O₂. Compared with the control group, the activities of GSH, SOD, CAT, and TrxR in the cultured supernatant were significantly reduced after 1000 μM H₂O₂ treatment for 2 hours (P < .05) (Figure 6a–6d). After adding 80 and 100 μg/mL CHS-IVa for 6 hours, the activities of GSH, SOD, CAT, and TrxR in the supernatant all showed an upward trend, and the activities of TrxR, GSH, and CAT increased significantly (P < .05). Next, changes in MDA content were detected. Compared with the control group, the content of MDA in the culture supernatant of the 1000 μM H₂O₂ group increased (Figure 6e). However, compared with the H₂O₂ single treatment group, the MDA content in the culture supernatant of cells treated with 80 and 100 μg/mL CHS-IVa showed a decreasing trend.

Figure 6.

Effect of CHS-IVa on CAT (a), SOD (b), GSH (c), TrxR (d) activity, and MDA (e) content in the culture supernatant of IPEC-J2 cells treated with H₂O₂. Data are presented as mean ± SD. *P < .05 was statistically significant compared to the control group.

CHS-IVa Reduced H₂O₂-Induced Up-Regulation of Gene Expression of Inflammatory-Related Factors in IPEC-J2 Cells

Next, we investigated the effect of different concentrations of CHS-IVa on inflammatory-related factors in IPEC-J2 cells. Compared with the control group, the relative expression of IL-6 and IL-1α mRNA in H₂O₂ showed an upward trend, and the increase of IL-6 was significant (P < .05) (Figure 7a and b). After the addition of 80 and 100 μg/mL CHS-IVa, the relative expression of IL-6 mRNA showed a downward trend with a significant difference (P < .05), while the relative expression of IL-1α showed a downward trend only after the addition of 80 μg/mL CHS-IVa. After treatment with 1000 μM H₂O₂, the relative expression of TNF-α mRNA in the cells showed an upward trend (Figure 7c). After treatment with CHS-IVa at 80 and 100 μg/mL, the relative expression of TNF-α showed a downward trend. Similarly, compared to the control group, the relative expression of P65 and NF-κB mRNA in the cells of the H₂O₂ single treatment group showed an upward trend (Figure 7d and e). After adding CHS-IVa at concentrations of 80 and 100 μg/mL, the relative expression of P65 and NF-κB mRNA in the cells showed a downward trend, and 100 μg/mL CHS-IVa downregulated the expression of P65 and NF-κB mRNA more obviously.

Figure 7.

Effect of CHS-IVa on the relative expression of IL-6 (a), IL-1α (b), TNF-α (c), NF-κB (e), and P65 (d) dmRNA in IPEC-J2 cells treated with H₂O₂. Data are presented as mean ± SD. *P < .05 was statistically significant compared to the control group.

Discussion

As the main organ of nutrient digestion and absorption in the body, the intestine is also the largest immune defense organ in the animal's body, and is essential for maintaining the health and growth of livestock and poultry.³¹ Intestinal injury caused by a variety of factors can lead to intestinal mucosal damage and dysfunction.^3233-34 Oxidative stress is a key factor in gut barrier disruption in intestinal diseases.³⁵ Many natural herbs have a protective effect on the intestines. Post-treatment with ginsenoside Rb1 has been shown to improve the mesenteric venous albumin leakage induced by lipopolysaccharide, thereby protecting the intestine.³⁶ There are also studies indicating that Ginsenoside RD can prevent experimental colitis by reducing the accumulation of leukocytes and downregulating multiple pro-inflammatory cytokines.³⁷ As a natural active ingredient in Panax japonicus saponin, CHS-IVa has various medicinal effects such as anti-injury and anti-apoptosis.³⁸ The results showed that 50 to 200 μg/mL CHS-IVa could promote the growth of IPEC-J2 cells within 9 hours, while 400 μg/mL of CHS-IVa reduced cell activity. At 48 hours, 200 μg/mL of CHS-IVa showed toxic effects on cells, reducing cell viability. Then, the cell viability of cells treated with different concentrations of CHS-IVa was subsequently detected for 3 and 6 hours, and it was found that the cell viability also increased. In Anmin Shao's study, concentrations of CHS-IVa at 40 and 50 μM significantly reduced the viability of primary neurons within 6 hours, while CHS-IVa at 10, 20, and 30 μM had no significant effect on cell viability.³⁹ It was shown that high concentrations of CHS-IVa can inhibit cell activity. We also showed that CHS-IVa has a repairing effect on intestinal epithelial cell injury through scratch experiments. Studies have shown that CHS-IVa can promote cell repair by inhibiting the migration of ovarian cancer cells.⁴⁰

Many studies have shown that CHS-IVa has antioxidant effects.¹² Oxidative stress refers to the phenomenon that interferes with the oxidative balance of the intracellular redox system due to the excessive production of ROS in the cell under the influence of external harmful factors.⁴¹ There are many natural Chinese herbs that have antioxidant effects.^42,43 They play an important role in antioxidants by changing the activities of SOD, CAT, GSH, and other antioxidant enzymes to scavenge reactive oxygen radicals produced in the process of cellular oxidative metabolism.⁴⁴ The results of this study showed that the decrease in SOD activity, TrxR activity, CAT activity, and GSH activity induced by H₂O₂ could be alleviated after CHS-IVa treatment at 80 and 100 μg/mL, which indicating that intracellular reactive oxygen radicals were controlled under CHS-IVa treatment. Wang's findings also show that CHS-IVa at 10, 20, and 40 μM increased SOD and GSH activity in pancreatic β-cell.⁴⁵ At the same time, our study also showed a decrease in LDH levels in the cell supernatant. Studies have shown that CHS-IVa can reduce LDH release.^45,46 In conclusion, we know that CHS-IVa has an antioxidant effect on IPEC that can relieve or inhibit intestinal damage caused by oxidative stress and maintain the normal life activities of the body. It is speculated that CHS-IVa can be used for further research on potential antioxidants.

The body's oxidative stress response pathway is closely related to inflammation and cytokine secretion. As pro-inflammatory factors, IL-1α and IL-6 are involved in the inflammatory and immune response of cells.^47,48 Studies have shown that inhibition of the NF-κB pathway can exert anti-inflammatory effects.⁴⁹ Yuan's research showed that CHS-IVa can reduce the expression of NF-κB and IL-1β in mouse epididymal adipose tissue and macrophages induced by high fat.⁵⁰ Meanwhile, it was reported that CHS-IVa could suppress the levels of IL-1β, IL-6, and IL-10 in mouse macrophages by lipopolysaccharide.⁵¹ Our study found that CHS-IVa attenuated the H₂O₂-induced increase in the relative expression of IL-6, P65, and NF-κB in IPEC. It shows that CHS-IVa has a certain protective effect on intestinal inflammation. NF-κB is the key to inflammation and oxidative stress. In this study, we found that CHS-IVa can alleviate the upregulation of NF-kB caused by hydrogen peroxide, but we did not conduct in-depth experiments on regulating molecular mechanisms related to oxidative stress and inflammation, which requires further research in the future.

Conclusions

The concentration of 80 and 100 μg/mL CHS-IVa can enhance the viability of IPEC-J2 cells, promote the repair of cell scribing damage, and alleviate the decline of cell activity caused by H₂O₂. It can also slow down the decrease of SOD, TrxR, CAT, and GSH activity, as well as the increase of expression of inflammatory cytokines IL-6, P65, and NF-κB caused by H₂O₂. These findings provide fundamental research for CHS-IVa as an effective anti-injury drug.

Footnotes

Declaration of Conflicting Interests

The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Ethical Approval

Not applicable.

Funding

The authors disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was supported by the Natural Science Foundation of Hunan Province, China (2022JJ30022, 2022JJ30293) and the Hunan Province Technology Breakthrough Project of 2021 for the open competition mechanism to select the best candidates (2021NK1030).

Statement of Human and Animal Rights

Not applicable.

Statement of Informed Consent

Not applicable.

ORCID iD

Qing Liu

References

Zhang

Wang

Zeng

, et al. Resource investigation of traditional medicinal plant Panax japonicus (T.Nees) C.A. Mey and its varieties in China. J Ethnopharmacol. 2015;166:79-85. https://doi.org/10.1016/j.jep.2015.02.051

Zou

Zhu

Tohda

Cai

Komatsu

. Dammarane-type triterpene saponins from Panax japonicus. J Nat Prod. 2002;65(3):346-351. https://doi.org/10.1021/np010354j

Jie

Chun-Yan

Jing-Ping

, et al. Immunoregulation on mice of low immunity and effects on five kinds of human cancer cells of Panax japonicus polysaccharide. Evid Based Complement Alternat Med. 2015;2015:839697. https://doi.org/10.1155/2015/839697

Chen

, et al. Chain conformation and anti-tumor activity of derivatives of polysaccharide from Rhizoma Panacis Japonici. Carbohydr Polym. 2014;105:308-316. https://doi.org/10.1016/j.carbpol.2014.01.089

Wang

Xie

Liu

, et al. Panax japonicus and chikusetsusaponins: a review of diverse biological activities and pharmacology mechanism. Chin Herb Med. 2021;13(1):64-77. https://doi.org/10.1016/j.chmed.2020.12.003

Wang

, et al. Inhibitory effects of Chikusetsusaponin IVa on lipopolysaccharide-induced pro-inflammatory responses in THP-1 cells. Int J Immunopathol Pharmacol. 2015;28(3):308-317. https://doi.org/10.1177/0394632015589519

Duan

Yin

Cui

, et al. Chikusetsu Saponin IVa Ameliorates cerebral ischemia reperfusion injury in diabetic mice via adiponectin-mediated AMPK/GSK-3beta pathway in vivo and in vitro. Mol Neurobiol. 2016;53(1):728-743. https://doi.org/10.1007/s12035-014-9033-x

Xin

Yuan

Liu

Zhang

Yuan

. MiR-155/GSK-3beta mediates anti-inflammatory effect of Chikusetsusaponin IVa by inhibiting NF-kappaB signaling pathway in LPS-induced RAW264.7 cell. Sci Rep. 2020;10(1):18303. https://doi.org/10.1038/s41598-020-75358-1

Cui

, et al. Insulinotropic effect of Chikusetsu saponin IVa in diabetic rats and pancreatic beta-cells. J Ethnopharmacol. 2015;164:334-339. https://doi.org/10.1016/j.jep.2015.02.032

10.

Zhang

Cui

, et al. Chikusetsu saponin IVa regulates glucose uptake and fatty acid oxidation: implications in antihyperglycemic and hypolipidemic effects. J Pharm Pharmacol. 2015;67(7):997-1007. https://doi.org/10.1111/jphp.12392

11.

Rattanathongkom

Lee

Hayashi

, et al. Evaluation of chikusetsusaponin IVa isolated from Alternanthera philoxeroides for its potency against viral replication. Planta Med. 2009;75(8):829-835. https://doi.org/10.1055/s-0029-1185436

12.

Duan

Yin

Wei

, et al. Chikusetsu saponin IVa confers cardioprotection via SIRT1/ERK1/2 and Homer1a pathway. Sci Rep. 2015;5:18123. https://doi.org/10.1038/srep18123

13.

Arrieta

Bistritz

Meddings

. Alterations in intestinal permeability. Gut. 2006;55(10):1512-1520. https://doi.org/10.1136/gut.2005.085373

14.

Suzuki

. Regulation of intestinal epithelial permeability by tight junctions. Cell Mol Life Sci. 2013;70(4):631-659. https://doi.org/10.1007/s00018-012-1070-x

15.

Liang

Zhuo

Guo

, et al. SIRT1/PGC-1 Pathway activation triggers autophagy/mitophagy and attenuates oxidative damage in intestinal epithelial cells. Biochimie. 2020;170:10-20. https://doi.org/10.1016/j.biochi.2019.12.001

16.

Cao

Xiao

, et al. AMPK-PINK1/Parkin mediated mitophagy is necessary for alleviating oxidative stress-induced intestinal epithelial barrier damage and mitochondrial energy metabolism dysfunction in IPEC-J2. Antioxidants (Basel). 2021;10(12):2010. https://doi.org/10.3390/antiox10122010

17.

Mariani

Sena

Roncucci

. Inflammatory pathways in the early steps of colorectal cancer development. World J Gastroenterol. 2014;20(29):9716-9731. https://doi.org/10.3748/wjg.v20.i29.9716

18.

McCord

Fridovich

. The utility of superoxide dismutase in studying free radical reactions. I. Radicals generated by the interaction of sulfite, dimethyl sulfoxide, and oxygen. J Biol Chem. 1969;244(22):6056-6063.

19.

MILLS

. Hemoglobin catabolism. I. Glutathione peroxidase, an erythrocyte enzyme which protects hemoglobin from oxidative breakdown. J Biol Chem. 1957;229(1):189-197.

20.

Demircigil

Gul

Gokturk

, et al. Thymoquinone played a protective role against tartrazine-induced hepatotoxicity. Iran J Basic Med Sci. 2023;26(1):99-106. https://doi.org/10.22038/IJBMS.2022.67341.14763

21.

Ghasemi

Evazalipour

Peyghanbari

, et al. Isolation and structure elucidation of the compounds from Teucrium hyrcanicum L. and the investigation of cytotoxicity, antioxidant activity, and protective effect on hydrogen peroxide-induced oxidative stress. BMC Complement Med Ther. 2023;23(1):447. https://doi.org/10.1186/s12906-023-04262-8

22.

Dun

Liu

Chen

, et al. Regulatory effects of saponins from Panax japonicus on colonic epithelial tight junctions in aging rats. J Ginseng Res. 2018;42(1):50-56. https://doi.org/10.1016/j.jgr.2016.12.011

23.

Wang

, et al. Therapeutic effect of the total saponin from Panax Japonicus on experimental autoimmune encephalomyelitis by attenuating inflammation and regulating gut microbiota in mice. J Ethnopharmacol. 2023;315:116681. https://doi.org/10.1016/j.jep.2023.116681

24.

Liu

Zhao

Chen

, et al. Polyacetylenic oleanane-type triterpene saponins from the roots of Panax japonicus. J Nat Prod. 2016;79(12):3079-3085. https://doi.org/10.1021/acs.jnatprod.6b00748

25.

Yuan

Wang

, et al. Fumonisin B (1) inhibits cell proliferation and decreases barrier function of swine umbilical vein endothelial cells. Toxins (Basel). 2021;13(12):863. https://doi.org/10.3390/toxins13120863

26.

Ran

Zeng

Liao

Liu

. The effect of miR-503-5p on the proliferation, invasion, migration and epithelial interstitium of cervical cancer HeLa cells via targeting E2 F3. Sichuan Da Xue Xue Bao Yi Xue Ban. 2020;51(2):178-184. https://doi.org/10.12182/20200360501

27.

. Regulating the expression of Ginsenoside Rg1 on the surface antigen proteinson reactive astrocyte and study its function in spinal cord injury. Zhongguo Lin Chuang Yao Li Xue Za Zhi. 2019;35(11):1153-1155.

28.

Yang

Ling

Hua

, et al. IL-6R blockade by chikusetsusaponin IVa butyl ester inhibits Th17 cell differentiation and ameliorates collagen-induced arthritis. Cell Mol Immunol. 2021;18(6):1584-1586. https://doi.org/10.1038/s41423-021-00651-6

29.

Cai

Zhu

Chen

, et al. X/XO or H₂O₂ induced IPEC-J2 cell as a new in vitro model for studying apoptosis in post-weaning piglets. Cytotechnology. 2016;68(4):713-724. https://doi.org/10.1007/s10616-014-9823-z

30.

Fotakis

Timbrell

. In vitro cytotoxicity assays: comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride. Toxicol Lett. 2006;160(2):171-177. https://doi.org/10.1016/j.toxlet.2005.07.001

31.

Chen

Tang

Sun

. Regulatory functions of fatty acids with different chain lengths on the intestinal health in pigs and relative signaling pathways. Curr Protein Pept Sci. 2019;20(7):674-682. https://doi.org/10.2174/1389203720666190514120023

32.

Pan

Zhao

Farouk

, et al. Integrins were involved in soybean agglutinin induced cell apoptosis in IPEC-J2. Int J Mol Sci. 2018;19(2):587. https://doi.org/10.3390/ijms19020587

33.

Chen

Zhou

Yuan

, et al. Effect of fumonisin B (1) on oxidative stress and gene expression alteration of nutrient transporters in porcine intestinal cells. J Biochem Mol Toxicol. 2021;35(4):e22706. https://doi.org/10.1002/jbt.22706

34.

Chen

Ding

, et al. Comparing the influence of high doses of different zinc salts on oxidative stress and energy depletion in IPEC-J2 cells. Biol Trace Elem Res. 2020;196(2):481-493. https://doi.org/10.1007/s12011-019-01948-4

35.

Bhattacharyya

Chattopadhyay

Mitra

Crowe

. Oxidative stress: an essential factor in the pathogenesis of gastrointestinal mucosal diseases. Physiol Rev. 2014;94(2):329-354. https://doi.org/10.1152/physrev.00040.2012

36.

Zhang

Sun

Liu

, et al. Ginsenoside Rb1 ameliorates lipopolysaccharide-induced albumin leakage from rat mesenteric venules by intervening in both trans- and paracellular pathway. Am J Physiol Gastrointest Liver Physiol. 2014;306(4):G289-G300. https://doi.org/10.1152/ajpgi.00168.2013

37.

Yang

Guo

Wang

, et al. Ginsenoside Rd attenuates the inflammatory response via modulating p38 and JNK signaling pathways in rats with TNBS-induced relapsing colitis. Int Immunopharmacol. 2012;12(2):408-414. https://doi.org/10.1016/j.intimp.2011.12.014

38.

Cui

Duan

Chu

, et al. Chikusetsu saponin IVa protects pancreatic beta cell against intermittent high glucose-induced injury by activating Wnt/beta-catenin/TCF7L2 pathway. Aging (Albany NY). 2020;12(2):1591-1609. https://doi.org/10.18632/aging.102702

39.

Shao

Fei

Feng

Weng

. Chikusetsu saponin IVa alleviated sevoflurane-induced neuroinflammation and cognitive impairment by blocking NLRP3/caspase-1 pathway. Pharmacol Rep. 2020;72(4):833-845. https://doi.org/10.1007/s43440-020-00078-2

40.

Chen

Meng

, et al. Chikusetsusaponin IVa methyl ester induces G1 cell cycle arrest, triggers apoptosis and inhibits migration and invasion in ovarian cancer cells. Phytomedicine. 2016;23(13):1555-1565. https://doi.org/10.1016/j.phymed.2016.09.002

41.

Pizzino

Irrera

Cucinotta

, et al. Oxidative stress: harms and benefits for human health. Oxid Med Cell Longev. 2017;2017:8416763. https://doi.org/10.1155/2017/8416763

42.

Cao

Wang

Yan

, et al. Curcumin ameliorates oxidative stress-induced intestinal barrier injury and mitochondrial damage by promoting Parkin dependent mitophagy through AMPK-TFEB signal pathway. Free Radic Biol Med. 2020;147:8-22. https://doi.org/10.1016/j.freeradbiomed.2019.12.004

43.

Zhuang

Wang

, et al. Resveratrol attenuates oxidative stress-induced intestinal barrier injury through PI3K/Akt-mediated Nrf2 signaling pathway. Oxid Med Cell Longev. 2019;2019:7591840. https://doi.org/10.1155/2019/7591840

44.

Deavall

Martin

Horner

Roberts

. Drug-induced oxidative stress and toxicity. J Toxicol. 2012;2012:645460. https://doi.org/10.1155/2012/645460

45.

Wang

Duan

Jia

, et al. IRS-2/Akt/GSK-3beta/Nrf2 pathway contributes to the protective effects of Chikusetsu Saponin IVa against lipotoxicity. Oxid Med Cell Longev. 2021;2021:8832318. https://doi.org/10.1155/2021/8832318

46.

Fang

Han

Zhao

Luo

. Chikusetsu saponin IVa attenuates isoflurane-induced neurotoxicity and cognitive deficits via SIRT1/ERK1/2 in developmental rats. Am J Transl Res. 2017;9(9):4288-4299.

47.

Tanaka

Narazaki

Kishimoto

. IL-6 in inflammation, immunity, and disease. Cold Spring Harb Perspect Biol. 2014;6(10):a016295. https://doi.org/10.1101/cshperspect.a016295

48.

Di Paolo

Shayakhmetov

. Interleukin 1alpha and the inflammatory process. Nat Immunol. 2016;17(8):906-913. https://doi.org/10.1038/ni.3503

49.

Lawrence

. The nuclear factor NF-kappaB pathway in inflammation. Cold Spring Harb Perspect Biol. 2009;1(6):a001651. https://doi.org/10.1101/cshperspect.a001651

50.

Yuan

Liu

Wang

, et al. Chikusetsu saponin IVa ameliorates high fat diet-induced inflammation in adipose tissue of mice through inhibition of NLRP3 inflammasome activation and NF-kappaB signaling. Oncotarget. 2017;8(19):31023-31040. https://doi.org/10.18632/oncotarget.16052

51.

Lei

Yuan

Chen

. Inhibition of chikusetsusaponin IVa on inflammatory responses in RAW264.7 cell line via MAPK pathway. Z Naturforsch C J Biosci. 2021;76(3-4):103-110. https://doi.org/10.1515/znc-2019-0107

Protective Effect of Chikusetsu saponin IVa on Hydrogen Peroxide Induced Injury of IPEC-J2 Cells

Abstract

Keywords

Introduction

Materials and Methods

Experimental Reagents

Drug Configuration

Cell and Cell Culture

Cell Viability Assay

Cell Scratch Experiment

Measurement of LDH, CAT, SOD, GSH, TrxR, and MDA

qPCR

Statistics and Analysis

Results

Different Concentrations of H2O2 Reduced the Viability of IPEC-J2 Cells

Different Concentrations of CHS-IVa Regulated the Viability of IPEC-J2 Cells

Different Concentrations of CHS-IVa Repaired the Scratch Damage of IPEC-J2 Cells

CHS-IVa Repaired the Damage of IPEC-J2 Cells after H2O2 Treatment

Regulation of CHS-IVa on the Changes of MDA Content and Antioxidant Enzyme Activity in IPEC-J2 Cells Treated With H2O2

CHS-IVa Reduced H2O2-Induced Up-Regulation of Gene Expression of Inflammatory-Related Factors in IPEC-J2 Cells

Discussion

Conclusions

Footnotes

Declaration of Conflicting Interests

Ethical Approval

Funding

Statement of Human and Animal Rights

Statement of Informed Consent

ORCID iD

References

Different Concentrations of H₂O₂ Reduced the Viability of IPEC-J2 Cells

CHS-IVa Repaired the Damage of IPEC-J2 Cells after H₂O₂ Treatment

Regulation of CHS-IVa on the Changes of MDA Content and Antioxidant Enzyme Activity in IPEC-J2 Cells Treated With H₂O₂

CHS-IVa Reduced H₂O₂-Induced Up-Regulation of Gene Expression of Inflammatory-Related Factors in IPEC-J2 Cells