The action of seven polyhydroxylated sterol mono- and disulfates (1-7), isolated from ophiuroids, on innate and adaptive immunity was examined in in vitro and in vivo experiments. At least, three of them (1, 2 and 4) increased the functional activities of neutrophils, including levels of oxygen-dependent metabolism, adhesive and phagocytic properties, and induced the expression of pro-inflammatory cytokines TNF-α and IL-8. Compound 4 was the most active for enhancing the production of antibody forming cells in the mouse spleen.
d'AuriaMV, RiccioR, MinaleL, La BarreS, PussetJ. (1987) Novel marine steroid sulfates from Pacific ophiuroids. The Journal of Organic Chemistry, 52, 3947–3952.
9.
ShubinaLK, FedorovSN, LevinaEV, AndriyaschenkoPV, KalinovskyAI, StonikVA, SmirnovIS. (1998) Comparative study on polyhydroxylated steroids from echinoderms. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 119, 505–511.
10.
d'AuriaMV, RiccioR, UriarteE, MinaleL, TanakaJ, HigaT. (1989) Isolation and structure elucidation of seven new polyhydroxylated sulfated sterols from the ophiuroid Ophiolepis superba.The Journal of Organic Chemistry, 54, 234–239.
11.
FedorovSN, LevinaEV, KalinovskyAI, DmitrenokPS, StonikVA. (1994) Sulfated steroids from Pacific brittle stars Ophiopholis aculeata, Ophiura sarsi and Stegophiura brachiactis.Journal of Natural Products, 57, 1631–1637.
12.
KapustinaII, MakarievaTN, StonikVA, KalinovskyAI. (1993) Steroids from ophiuroids. III. Sulfated steroids from Gorgonocephalus caryi.Chemistry of Natural Compounds, 29, 256–256.
13.
LevinaEV, AndriyaschenkoPV, StonikVA, KalinovskyAI. (1996). Ophiuroid - type steroids in starfish of the genus Pteraster. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 114, 49–52.
14.
LevinaEV, AndriyaschenkoPV, KalinovskyAI, StonikVA. (1998). New ophiuroid - type steroids from the starfish Pteraster tesselatus.Journal of Natural Products, 61, 1423–1426.
15.
ElyakovGB, StonikVA, FedorovSN. (1992) New polar water-soluble cytotoxins from Echinoderms. Toxicon, 30, 505–505.
16.
AmininDL, AgafonovaIG, FedorovSN. (1995) Biological activity of disulfated polyhydroxysteroids from the Pacific brittle star Ophiopholis aculeata.Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology, 112, 201–204.
17.
AgafonovaIG, AmininDL, ShubinaLK, FedorovSN. (2002) Influence of polyhydroxysteroids on [Ca2+](i). Steroids, 67, 695–701.
18.
JerneNK, NordinAA. (1963) Plaque formation in agar by single antibody-producing cells. Science, 140, 405.
19.
LagrangePH, MackanessGB, MillerTE. (1974) Influence of dose and route of antigen injection on the immunological induction of T cells. The Journal of Experimental Medicine, 139, 528–542.
SovaVV, LevinaEV, AndriyashchenkoPV, FedorovSN, ElyakovaLA. (1994) Effect of polyhydroxysteroids from starfish and brittle stars on the activity of β-l,3-glucanases. Chemistry of Natural Compounds, 30, 599–602.