The anthraquinone profile, antioxidant and antimicrobial activities as well as the total phenol and total flavonoid contents were determined in methanol extracts of the barks of Rhamnus catharticus L. and R. orbiculatus Bornm. The most abundant anthraquinone derivatives in R. catharticus were physcion (67.8%) and emodin (26.2%), while R. orbiculatus contained mostly physcion (81.3%) and chrysophanol (14.6%). R. catharticus displayed better activity in the β-carotene–linoleic acid assay, as well as chelating activity, whereas its activity in the reducing power assay was significantly lower than that of R. orbiculatus. Both methanol extracts showed antimicrobial activity against all microbial species tested (Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Candida albicans, Aspergillus niger, Microsporum gypseum) with MIC values either equal to or lower than 2.50 mg/mL. R. catharticus and R. orbiculatus contained several anthranoid aglycones and their bark extracts demonstrated notable antioxidant and antimicrobial properties. The results obtained indicate the medicinal potential of these two species.
MokiniZ, MarcovecchioML, ChiarelliF. (2010) Molecular pathology of oxidative stress in diabetic angiopathy: Role of mitochondrial and cellular pathways. Diabetes Research and Clinical Practice, 87, 313–321.
2.
ValkoM, RhodesCJ, MoncolaJ, IzakovicM, MazuraM. (2006) Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chemico-Biological Interactions, 160, 1–40.
3.
SinghU, JialalI. (2006) Oxidative stress and atherosclerosis. Pathophysiology, 13, 29–142.
4.
IssaAY, VolateSR, WargovichMJ. (2006) The role of phytochemicals in inhibition of cancer and inflammation: New directions and perspectives. Journal of Food and Composition and Analysis, 19, 405–419.
5.
Ben AmmarR, KilaniS, BouhlelI, EzziL, SkandraniI, BoubakerJ, SghaierMB, NaffetiA, MahmoudA, Chekir-GhediraL, GhediraK. (2008) Antiproliferative, antioxidant, and antimutagenic activities of flavonoid-enriched extracts from (Tunisian) Rhamnus alaternus L.: combination with the phytochemical composition. Drug and Chemical Toxicology, 31, 61–80.
6.
NgLT, LinCC, LuCM. (2007) Antioxidative effects of 6-methoxysorigenin and its derivatives from Rhamnus nakaharai. Chemical & Pharmaceutical Bulletin, 55, 382–384.
7.
Ben AmmarR, BouhlelI, ValentiK, SghaierMB, KilaniS, MariotteAM, Dijoux-FrancaMG, LaporteF, GhediraK, Chekir-GhediraL. (2007) Transcriptional response of genes involved in cell defense system in human cells stressed by H2O2 and pretreated with (Tunisian) Rhamnus alaternus extracts: combination with polyphenolic compounds and classic in vitro assays. Chemico-Biological Interactions, 168, 171–183.
8.
JiangJS, ShihCM, WangSH, ChenTT, LinCN, KoWC. (2006) Mechanisms of suppression of nitric oxide production by 3-O-methylquercetin in RAW 264.7 cells. Journal of Ethnopharmacology, 103, 281–287.
9.
KoWC, ShihCM, ChenMC, LaiYH, ChenJH, ChenCM, LinCN. (2004) Suppressive effects of 3-O-methylquercetin on ovalbumin-induced airway hyperresponsiveness. Planta Medica, 70, 1123–1127.
10.
GenoveseS, TammaroF, MenghiniL, CarlucciG, EpifanoF, LocatelliM. (2010) Comparison of three different extraction methods and HPLC determination of the anthraquinones aloe-emodine, emodine, rheine, chrysophanol and physcione in the bark of Rhamnus alpinus L. (Rhamnaceae). Phytochemical Analysis, 21, 261–267.
11.
LocatelliM, TammaroF, MenghiniL, CarlucciG, EpifanoF, GenoveseS. (2009) Anthraquinone profile and chemical fingerprint of Rhamnus saxatilis L. from Italy. Phytochemistry Letters, 2, 223–226.
12.
AmarowiczR, PeggRB, Rahimi-MoghaddamP, BarlB, WeilJA. (2004) Free-radical scavenging capacity and antioxidant activity of selected plant species from the Canadian prairies. Food Chemistry, 84, 551–562.
13.
BloisMS. (1958) Antioxidant determinations by the use of a stable free radical. Nature, 181, 1199–1201.
14.
GaleyJB. (1997) Potential use of iron chelators against oxidative damage. Advances in Pharmacology, 38, 167–203.
PandeyMB, SinghAK, SinghU, SinghS, PandeyVB. (2008) A new chalcone glycoside from Rhamnus nepalensis. Natural Product Research, 22, 1657–1659.
17.
OrhanI, ŞenolFS, GülpinarAR, KartalM, ŞekerogluN, DeveciM, KanY, ŞenerB. (2009) Acetylcholinesterase inhibitory and antioxidant properties of Cyclotrichium niveum, Thymus praecox subsp. caucasicus var. caucasicus, Echinacea purpurea and E. pallida. Food and Chemical Toxicology, 47, 1304–1310.
18.
YenGC, ChenHY. (1995) Antioxidant activity of various tea extracts in relation to their antimutagenicity. Journal of Agricultural and Food Chemistry, 43, 27–37.
19.
Zovko KončićM, KremerD, GruzJ, StrnadM, BiševacG, KosalecI, ŠamecD, Piljac-ŽegaracJ, KarlovićK. (2010) Antioxidant and antimicrobial properties of Moltkia petraea (Tratt.) Griseb. flower, leaf and stem infusions. Food and Chemical Toxicology, 48, 1537–1542.
20.
DeckerEA, WelchB. (1990) Role of ferritin as lipid oxidation catalyst in muscle food, Food Chemistry, 38, 674–677.
21.
SingletonVL, OrthoferR, Lamuela-RaventosRM. (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin–Ciocalteau reagent. Methods in Enzymology, 299, 152–178.
22.
KumazawaS, HamasakaT, NakayamaT. (2004) Antioxidant activity of propolis of various geographic origins. Food Chemistry, 84, 329–339.
23.
National Committee for Laboratory Standards (NCCLS). (1997) Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically, 4th Ed., Approved standards, NCCLS document M7-A4. National Committee for Clinical Laboratory Standards, Villanova, PA.
24.
National Committee for Laboratory Standards (NCCLS). (1997) Methods for dilution antimicrobial susceptibility testing of yeasts, 4th Ed., Approved standards, NCCLS document M-27A, National Committee for Clinical Laboratory Standards, Villanova, PA.
