Chromatographic separation of the ethyl acetate extract of roots of Polyalthia cerasoides has led to the isolation of the new compound, 6,8-dihydroxy-7-methoxy-1-methyl-azafluorenone. This compound exhibited potent cytotoxic activities with IC50 values in the range of 2.64-3.58 μg.mL−1 for A549, GLC4 and GLC4/Adr cells, but was not recognized by ABCC1/MRP1 protein. The compound also showed very strong inhibition of M. tuberculosis using a broth microdilution method, with an MIC value of 0.78 μg.mL−1, which was equal to that of ofloxacin, one of the four antibiotic drugs used as a positive control.
SamHV, NanthavongK, KeßlerPja. (2004) Trees of Laos and Vietnam. Blumea, 49201-349.
3.
Zafra-PoloMC, GonzálezMC, TormoJR, EstornellE, CortesD. (1996) Polyalthidin: New prenylated benzopyran inhibitor of the mammalian mitochondrial respiratory chain. Journal of Natural Products, 59913-916;
4.
GonzálezMC, Zafra-PoloMC, BlázquezMpa, SerranoA, CortesD. (1997) Cerasodine and cerasonine: New oxoprotoberberine alkaloids from Polyalthia cerasoides. Journal of Natural Products, 60108-110;
5.
KanokmedhakulS, KanokmedhakulK, LekphromR. (2007) Bioactive constituents of the roots of Polyalthia cerasoides. Journal of Natural Products, 701536-1538;
6.
RavikumarYS, MahadevanKM, ManjunathaH, SatyanarayanaND. (2010) Antiproliferative, apoptotic and antimutagenic activity of isolated compounds from Polyalthia cerasoides seeds. Phytomedicine, 17513-518;
7.
TegosG, StermitzFR, LomovskayaO, LewisK. (2002) Multidrug pump inhibitor uncover remarkable activity of plant antimicrobials. Antimicrobial Agents and Chemotherapy, 463133-3141.
8.
De BoerHJ, KoolA, BrobergA, MzirovWR, HedbergI, LevenforsJJ. (2005) Antifungal and antibacterial activity of some herbal remedies from Tanzania. Journal of Ethnopharmacology, 96461-469;
9.
ChenCY, ChangFR, ShihYC. (2000) Cytotoxic constituents of Polyalthia longifolia var pendula. Journal of Natural Products, 631475-1478.
10.
DudukuK, RosalamS, RajeshN. (2010) A review of the antioxidant potential of medicinal plant species. Food and Bioproducts Processing. (In Press);
11.
LoeDW, DeeleyRG, ColeSpc. (1996) Biology of the multidrug resistance-associated protein, mrp. European Journal of Cancer, 32945-957;
12.
JurankaPF, ZastawnyRL, LingV. (1989) P-glycoprotein: Multidrug-resistance and a superfamily of membrane- associated transport proteins. Journal of FASEB, 32583-2592;
13.
RavikumarYS, MahadevanKM, KumaraswamyMN, VaidyaYP, ManjunathaH, KumarVS. (2008) Antioxidant, cytotoxic and genotoxic evaluation of alcoholic extract of Polyalthia cerasoides (Roxb.) Bedd. Environmental Toxicology and Pharmacology, 26142-146;
14.
ChangsenC, FranzblauSG, PalittapongarnpimP. (2003) Improved green fluorescent protein reporter gene-based microplate screening for antituberculosis compounds by utilizing an acetamidase promoter. Antimicrobial Agents and Chemotherapy, 473682-3687.