K + ATP Channels-Independent Analgesic Action of Crotalus durissus cumanensis venom

Abstract

The effect was investigated of the K⁺ channel blocker, glibenclamide, on the ability of Crotalus durissus cumanensis venom (CDCM) to promote peripheral antinociception. This was measured by formalin-induced nociception in male Swiss mice. CDCM (200 and 300 μg/kg) produced an antinociceptive effect during phase 2 in the formalin test. The effect of CDCM (200 μg/kg) was unaffected by the ATP-sensitive K⁺ channel blocker glibenclamide (2 mg/kg). These results suggest that CDCM is effective against acute pain. However, the ATP-sensitive K⁺ channels pathway is not contribuable to the antinociceptive mechanism of CDCM.