Canavalia gladiata (CGL), C. maritima (ConM) and C. brasiliensis (ConBr) lectins were evaluated in nociception models. ConBr inhibited first (32%) and second (100%) phases of the formalin test; CGL inhibited only the first (74%) and ConM only the second (59%) phase. Hypernociception evaluated in the Von Frey test was inhibited by ConM (55%), CGL (41%) and ConBr (38%). Acetic acid-induced abdominal writhing was reduced by ConBr (66%), CGL (52%) and ConM (60%). ConBr and CGL effects were reversed by the lectin association with its ligand sugar. The antinociceptive activity of the structural homologous lectins was differentiated by potency, efficacy and mechanisms.
PeumansW.J., BarreA., HaoQ., RougéP., Van DammeE.J.M. (2000) Higher plants developed structurally different motifs to recognize foreign glycans. Trends in Glycoscience and Glycotechnology, 12, 83–101.
NunesB.S., RensonnetN.S., Dal-SeccoD., VieiraS.M., CavadaB.S., TeixeiraE.H., MouraT.R., TeixeiraC.S., Clemente-NapimogaJ.T., CunhaF.Q., NapimogaM.H. (2009) Lectin extracted from Canavalia grandiflora seeds presents potential anti-inflammatory and analgesic effects. Naunyn-Schmiedeberg's Archives of Pharmacology, 379, 609–616.
4.
DelatorreP., RochaB.A., SimõesR.C., Pereira-JúniorF.N., SilvaH.C., BezerraE.H., BezerraM.J., MarinhoE.S., GadelhaC.A., Santi-GadelhaT., FariasD.L., AssreuyA.M., Marques-DomingosG.F., NaganoC.S., CavadaB.S. (2011) Mass spectrometry and X-ray diffraction analysis of two crystal types of Dioclea virgata lectin: an antinociceptive protein candidate to structure/function analysis. Applied Biochemistry and Biotechnology, 164, 741–754.
5.
FigueiredoJ.G., da SilveiraB.F., BeserraI.G., TeixeiraC.S., LuzP.B., BezerraE.H., MotaM.R., AssreuyA.M., de Queiroz CunhaF., CavadaB.S., de AlencarN.M. (2009) Antinociceptive activity and toxicology of the lectin from Canavalia boliviana seeds in mice. Naunyn-Schmiedeberg's Archives of Pharmacology, 380, 407–414.
6.
HolandaF.R., Coelho-De-SouzaA.N., AssreuyA.M.S., Leal-CardosoJ.H., PiresA.F., NascimentoK.S., CavadaB.S., SantosC.F. (2009) Antinociceptive activity of lectins from Diocleinae seeds on acetic acid-induced writhing test in mice. Protein and Peptide Letters, 16, 1088–1092.
7.
PiresA.F., AssreuyA.M., LopesE.A., CeledônioN.R., SoaresC.E., RodriguesN.V., SousaP.L., BenevidesR.G., NaganoC.S., CavadaB.S., Leal-CardosoJ.H., Coelho-de-SouzaA.N., SantosC.F. (2011) Opioid-like antinociceptive effects of oral administration of a lectin purified from the seeds of Canavalia brasiliensis. Fundamental and Clinical Pharmacology. Sep 5. doi: 10.1111/j.1472–8206.2011.00987.x. [Epub ahead of print].
8.
TjφjlsenA., HoleK. (1997) Animal models of analgesia. In The pharmacology of pain.BessonM.J., DeckesonA. (Eds). Springer-Verlag, Berlin, Germany. 21–41.
9.
HunskaarS., HoleK. (1987) The formalin test in mice: dissociation between inflammatory and non-inflammatory pain. Pain, 30, 103–114.
10.
CunhaF.Q., PooleS., LorenzettiB.B., FerreiraS.H. (1992) The pivotal role of tumor necrosis factor alpha in the development of inflammatory hyperalgesia. British Journal of Pharmacology, 107, 660–664.
11.
DirosaM., GiroudJ.P., WilloughbyD.A. (1971) Studies of the mediators of the acute inflammatory response induced in rats in different sites by carrageenan and turpentine. The Journal of Pathology, 104, 15–29.
12.
SojaP.J., TaepavaraprukN., PangW., CairnsB.E., McerlaneS.A., FragosoM.C. (2002) Transmission through the dorsal spinocerebellar and spinoreticular tracts: wakefulness versus thiopental anesthesia. Anesthesiology, 97, 1178–1188.
13.
CavadaB.S., BarbosaT., ArrudaS., GrangeiroT.B., Barral-NettoM. (2001) Revisiting proteus: do minor changes in lectin structure matter in biological activity? Lessons from and potential biotechnological uses of the Diocleinae subtribe lectins. Current Protein and Peptide Science, 2, 123–135.
14.
StaiH.Y., ChenY.F., WuT.S. (1995) Anti-inflammatory and analgesic activities of extract from roots of Angelica pubescens. Planta Médica, 61, 1–8.
15.
IkedaY., UenoA., Naraba Oh-IshiH. (2001) Involvement of vaniloid receptor VR1 and prostanoids in the acid-induced writhing response of mice. Life Sciences, 69, 2911–2919.
16.
DelatorreP., RochaB.A., GadelhaC.A.A., Santi-GadelhaT., CajazeirasJ.B., SouzaE.P., NascimentoK.S. (2006) Crystal structure of a lectin from Canavalia maritima (ConM) in complex with trehalose and maltose reveals relevant mutation in ConA-like lectins. Journal of Structural Biology, 154, 280–286.
17.
BezerraG.A., OliveiraT.M., MorenoFBMB, SouzaE.P., RochaB.A.M., BenevidesR.G., DelatorreP., AzevedoW.F.Jr, CavadaB.S. (2007) Structural analysis of Canavalia maritima and Canavalia gladiata lectins complexed with different dimannosides: New insights into the understanding of the structure biological activity relationship in legume lectins. Journal of Structural Biology, 160, 168–176.
18.
GadelhaC.A.A., MorenoFBMB, Santi-GadelhaT., CajazeirasJ.B., RochaB.A.M., AssreuyA.M., MotaM.R.L., PintoN.V., MeirelesA.V.P., BorgesJ.C., FreitasB.T., CanduriF., SouzaE.P., DelatorreP., CriddleD.N., Azevedo-JúniorW.F., CavadaB.S. (2005) Native crystal structure of a nitric oxide-releasing lectin from the seeds of Canavalia maritima. Journal of Structural Biology, 152, 185–194.
19.
MoreiraR.A., CavadaB.S. (1984) Lectin from Canavalia brasiliensis (MART). Isolation, characterization and behavior during germination. Biologia Plantarum (Praha), 26, 113–120.
20.
MorenoF.B., DelatorreP., FreitasB.T., RochaB.A., SouzaE.P., FacóF., CanduriF., CardosoA.L., FreireV.N., Lima FilhoJ.L., SampaioA.H., CalveteJ.J., De AzevedoW.F.Jr, CavadaB.S. (2004) Crystallization and preliminary X-ray diffraction analysis of the lectin from Canavalia gladiata seeds. Acta Crystallographica Section D: Biological Crystallography, 60, 1493–1495.
21.
PerezG., PerezC., CavadaB.S., MoreiraR., RichardsonM. (1991) Comparison of the amino acid sequences of the lectins from seeds of Dioclea lehmanni and Canavalia maritima. Phytochemistry, 30, 2619–2621.
22.
Le BarsD., GozariuM., CaddenS.W. (2001) Animal models of nociception. Pharmacological Reviews, 53, 597–652.
