Microwave-based thermoacoustic tomography (TAT) and laser-based photoacoustic tomography (PAT) in a circular scanning configuration were both developed to image deeply seated lesions and objects in biological tissues. Because malignant breast tissue absorbs microwaves more strongly than benign breast tissue, cancers were imaged with good spatial resolution and contrast by TAT in human breast mastectomy specimens. Based on the intrinsic optical contrast between blood and chicken breast muscle, an embedded blood object that was 5 cm deep in the tissue was also detected using PAT at a wavelength of 1064 nm.
References
1.
ChaudharyS., MishraR., SwarupA., and ThomasJ.. Dielectric Properties of Normal & Malignant Human Breast Tissues at Radiowave and Microwave Frequencies. Indian J. Biochem. Biophys.21, 76–79 (1984).
2.
JoinesW., JirtleR., RafalM., and SchaefferD.. Microwave Power Absorption Differences Between Normal and Malignant Tissue. Radiation Oncology-Biology-Physics.6, 681–687 (1980).
3.
JoinesW., ZhangY., LiC., and JirtleR.. The Measured Electrical Properties of Normal and Malignant Human Tissues from 50–900 MHz. Medical Physics.21, 547–550 (1994).
4.
CheongW. F., PrahlS. A., and WelchA. J.. A Review of the Optical Properties of Biological Tissues. IEEE J. Quantum Elect.26, 2166–2185 (1990).
5.
LinJ. C.. On Microwave-Induced Hearing Sensation. IEEE Trans. Microwave Theory Tech.25, 605–613 (1977).
6.
SegelsteinD. J.. The Complex Refractive Index of Water. M. S. Thesis. Department of Physics, University of Missouri-Kansas City, Kansas City, Mo. (1981).
ZijlstraW. G., BuursmaA., and van AssendelftO. W.. Visible and Near Infrared Absorption Spectra of Human and Animal Haemoglobin.VSP, Netherlands (2000).
9.
JoinesW., JirtleR., RafalM., and SchaefferD.. Microwave Power Absorption Differences Between Normal and Malignant Tissue. Radiation Oncology-Biology-Physics6, 681–687 (1980).
10.
JoinesW., ZhangY., LiC., and JirtleR.. The Measured Electrical Properties of Normal and Malignant Human Tissues from 50–900 MHz. Medical Physics21, 547–550 (1994).
11.
BowenT., NasoniL., PiferA. E., and SembroskG. H.. In Proc. IEEE Ultrasonics Symposium2, 823–827 (1981).
12.
PowersJ. P., Ed. Acoustic Imaging.Plenum Publishing, New York (1982).
13.
OlsenR. G. and LinJ. C.. Acoustic Imaging of a Model of a Human Hand Using Pulsed Microwave Irradiation. Bioelectromagnetics.4, 397–400 (1983).
14.
LinJ. C. and ChanK. H.. Microwave Thermoelastic Tissue Imaging System Design. IEEE Trans. Microwave Theory Tech.32, 854–860 (1984).
15.
NasoniR. L., EvanoffG. A.Jr., HalversonP. G., and BowenT.. Thermoacoustic Emission by Deeply Penetrating Microwave Radiation. In Proc. IEEE Ultrasonics Symposium5, 633–637 (1984).
16.
ChanK. H. and LinJ. C.. Microwave-Induced Thermoacoustic Tissue Imaging. In Proc. Engineering in Medicine and Biology Society 10th Annual International Conference. pp. 445–446 (1988).
17.
KrugerR. A., LiuP-Y., and FangY.. Photoacoustic Ultrasound (PAUS)—Reconstruction Tomography. Med. Phys.22, 1605–1609 (1995).
18.
KrugerR. A., KopeckyK. K., AisenA. M., ReineckeD. R., KrugerG. A., and KiserW. L.Jr. Thermoacoustic CT with Radio Waves: A Medical Imaging Paradigm. Radiology.211, 275–278 (1999).
19.
KrugerR. A., ReineckeD. R., and KrugerG. A.. Thermoacoustic Computed Tomography-Technical Considerations. Med. Phys.26, 1832–1837 (1999).
20.
WangL.-H., ZhaoX., SunH., and KuG.. Microwave-Induced Acoustic Imaging of Biological Tissues. Rev. Sci. Instrum.70, 3744–3748 (1999).
21.
KuG. and WangL.-H.. Scanning Thermoacoustic Tomography in Biological Tissue. Med. Phys.27, 1195–1202 (2000).
22.
KuG. and WangL.-H.. Scanning Microwave-Induced Thermoacoustic Tomography: Signal, Resolution, and Contrast. Med. Phys.28, 4–10 (2001).
23.
KrugerR. A. and KiserW. L.Jr. Thermoacoustic CT of the Breast: Pilot Study Observations. Proc. SPIE4256, 1–5 (2001).
24.
KrugerR. A., StantzK., and KiserW. L.Jr. Thermoacoustic CT of the Breast. Proc. SPIE4682, 521–525 (2002).
25.
KostliK. P., FrenzM., BebieH., and WeberH. P.. Temporal Backward Projection of Optoacoustic Pressure Transients Using Fourier Transform Methods. Phys. Med. Biol.46, 1863–1872 (2001).
26.
XuM., and WangL.-H.. Time-Domain Reconstruction for Thermoacoustic Tomography in a Spherical Geometry. IEEE Trans. Med. Imag.21, 814–822 (2002).
27.
XuY., FengD., and WangL.-H.. Exact Frequency-Domain Reconstruction for Thermoacoustic Tomography: I. Planar Geometry. IEEE Trans. Med. Imag.21, 823–828 (2002).
28.
XuY., XuM., WangL.-H.. Exact Frequency-Domain Reconstruction for Thermoacoustic Tomography: II. Cylindrical Geometry. IEEE Trans. Med. Imag.21, 829–833 (2002).
29.
KolkmanR. G. M., HondebrinkE., SteenbergenW., de MulF. F. M.. In Vivo Photoacoustic Imaging of Blood Vessels Using an Extreme-Narrow Aperture Sensor. IEEE J. Sel. Top. Quantum Electron.9, 343–346, (2003).
30.
WangX., PangY., KuG., XieX., StoicaG., and WangL.-H.. Non-Invasive Laser-Induced Photoacoustic Tomography for Structural and Functional Imaging of the Brain In Vivo. Nat. Biotechnol.21, 803–806 (2003).
KuG., WangX., XieX., StoicaG., and WangL.-H.. Imaging of Tumor Angiogenesis in Rat Brains In Vivo by Photoacoustic Tomography. Applied Optics44, 770–775 (2005).
33.
EsenalievR. O., KarabutovA. A., and OraevskyA. A.. Sensitivity of Laser Opto-Acoustic Imaging in Detection of Small Deeply Embedded Tumors. IEEE J. Sel. Top. Quant.5, 981–988 (1999).
34.
KarabutovA. A., SavateevaE. V., and OraevskyA. A.. Optoacoustic Tomography: New Modality of Laser Diagnostic Systems. Laser Physics13, 711–723 (2003).
35.
OraevskyA. A., SavateevaE. V., SolomatinS. V., KarabutovA. A., AndreevV. G., GatalicaZ., KhamapiradT., and HenrichsP. M.. Optoacoustic Imaging of Blood for Visualization and Diagnostics of Breast Cancer. In Biomedical Optoacoustics III4618, 81–94. Ed., OraevskyA. A. (2002).
36.
KarabutovA. A., SavateevaE. V., PodymovaN. B., and OraevskyA. A.. Backward Mode Detection of Laser-Induced Wide-Band Ultrasonic Transients with Optoacoustic Transducer. J. Appl. Phys.87, 2003–2014 (2000).
37.
KarabutovA. A., SavateevaE. V., and OraevskyA. A.. Imaging of Layered Structures in Biological Tissues with Opto-Acoustic Front Surface Transducer. Proc. SPIE.3601, 284–295 (1999).
38.
MaslovK., StoicaG., and WangL.-H.. In Vivo Dark-Field Reflection-Mode Photoacoustic Microscopy. Optics Letters.30, 625–626 (2005).
39.
EsenalievR. O., TittelF. K., ThomsenS. L., FornageB., StellingC., KarabutovA. A., and OraevskyA. A.. Laser Optoacoustic Imaging for Breast Cancer Diagnostics: Limit of Detection and Comparison with X-ray and Ultrasound Imaging. Proc. SPIE.2979, 71–82 (1997).
40.
HoelenC. G. A. and de MulF. F. M.. Image Reconstruction for Photoacoustic Scanning of Tissue Structures. Appl. Opt.39, 5872–5883 (2000).
41.
WangX., PangY., KuG., StoicaG., and WangL.-H.. Three-Dimensional Laser-Induced Photoacoustic Tomography of the Mouse Brain with the Skin and Skull Intact. Optics Letters28, 1739–1741 (2003).
42.
KuG. and WangL.-H.. Deeply Penetrating Photoacoustic Tomography in Biological Tissues Enhanced with an Optical Contrast Agent. Optics Letters30, 507–509 (2005).
43.
TuchinV.. Tissue Optics, p. 33. SPIE, Bellingham, Washington (2000).
