Best practices for screening,testing,diagnosing,and treating patients with hepatitis D (delta) virus based on global expert review and recent guidelines

Abstract

Background

Hepatitis D virus (HDV) represents the most severe form of human viral hepatitis, associated with rapid progression to cirrhosis and increased liver-related mortality. Globally, an estimated 9-19 million individuals are anti-HDV positive. To ensure early detetion, current guidelines recommend screening all HBsAg-positive individuals or, at a minimum, those with defined risk factors.

Methods

This expert consensus paper updates the current landscape of HDV management. Recommendations were derived from a structured expert panel discussion, incorporating recent evidence and clinical guideline developments, with a focus on screening, diagnosis, and antiviral therapy.

Results

The panel emphasized the importance of systematic HDV screening in HBsAg-positive individuals. Therapeutic strategies aim at sustained HDV-RNA suppression and, ideally, HBV surface antigens (HBsAg) loss. Bulevirtide was recommended as a long-term monotherapy. Pegylated interferon alpha (PEG-IFNα), if used, should be limited to 48 weeks and tailored based on viral response and tolerability. Combination therapy with bulevirtide and PEG-IFNα may be considered in selected cases.

Conclusion

This consensus provides updated recommendations for the screening, diagnosis, and treatment of HDV infection, highlighting the role of bulevirtide and individualized therapeutic approaches. As the treatment landscape continues to evolve, combination regimens and novel agents currently under investigation may offer additional options in the near future.

Keywords

hepatitis D virus hepatitis delta epidemiology antiviral therapy bulevirtide

Introduction & methods

Hepatitis D Virus (HDV) is the most severe form of human viral hepatitis. A panel of 9 international experts evaluated the current landscape and issued recommendations based on guidelines, advancements in treatment options, and newly available literature.

Results & discussion

Natural history of HDV

Dr Mario Rizzetto first described the delta antigen 1977 as a subset of hepatitis B virus (HBV) infection with more severe liver disease.^1,2 The hepatitis D virion comprises the hepatitis D RNA genome, hepatitis D antigen (HDAg), and a lipoprotein envelope with HBsAg proteins.³ HDV requires HBV surface antigens (HBsAg) for cell entry and cellular RNA polymerases for replication.^3,4

Infection with HDV occurs under 2 circumstances: coinfection and superinfection (Figure 1). Coinfection occurs when patients are exposed to HBV and HDV simultaneously. These patients are at risk of developing acute liver failure (acute viral hepatitis), characterized by acute inflammation of the hepatic parenchyma or injury to hepatocytes. 95% of patients with HBV/HDV coinfection have viral clearance of both HBV and HDV. Superinfection occurs when patients with established chronic HBV (defined by the presence of HBsAg in serum) are exposed to HDV.⁴ Patients with HDV superinfections are those likely to develop chronic HDV infection.⁴

Figure 1.

Outcomes associated with superinfection and coinfection.^8,106 HBV: hepatitis B virus; HDV: hepatitis D virus.

Chronic HDV leads to severe viral hepatitis, with higher hepatocellular carcinoma (HCC) rates, faster cirrhosis progression, and higher mortality risk than chronic HBV monoinfection.^4,5 Spontaneous clearance of chronic HDV infection has been reported, with some anti-HDV-positive patients testing negative for HDV-RNA in subsequent visits.^6,7

Detectable HDV-RNA links to increased clinical events.⁹ While the interaction between hepatitis B and D is not fully understood, it may involve reciprocal interference of the replication of the two viruses. HDV interferes with HBV via IFN-dependent (upregulation of IFN-stimulated genes) and independent mechanisms, lowering HBV viremia.^3,10 HDV prevalence and outcomes in HIV/HBV coinfection remain underexplored (1.2–25%). Coinfection with HIV and persistent HBV replication increases liver risks, including decompensation, HCC, and death.^3,11

Epidemiology of HDV

HDV prevalence, epidemiological, and clinical features vary geographically. HDV prevalence generally correlates with HBV prevalence (e.g., >20% in parts of the Amazon basin, 20–40% in parts of Africa, <1% in North America).¹² However, some countries or regions (e.g., parts of China) experience higher HBV prevalence (7.18%) and relatively low HDV prevalence overall (2.63% HDV seroprevalence among HBV infections) with factors including HIV-1 infection influencing regional prevalence and distribution.¹³ Mongolia has the highest reported prevalence of HDV infection worldwide, affecting approximately 60% of HBsAg-positive individuals.¹⁴ HBV vaccination protects against HDV, reducing prevalence of HDV in high-income countries where vaccination has been implemented recently. However, HDV resurgence occurs in high-income countries due to risky behaviors and immigration.^3,15 HDV seroprevalence peaks in high-risk areas in Africa and Asia, Mongolia is a typical example of a high endemic area for both HBV and HDV.^16–18 Up to 10.6% of chronic HBV patients could be coinfected with HDV.³ In different epidemiological studies, HDV infections vary from 12 to 72 million people worldwide.^19–21, The prevalence of heterogeneity impacts clinical interpretation of the data. Since epidemiological data across the world are highly variable and HDV testing is lacking in many regions, it is impossible to determine the exact prevalence of HDV infections globally, highlighting the need for more surveillance data.^17,22

Of 8 known HDV genotypes, HDV-1 is distributed worldwide, whereas HDV-2 to HDV-8 are seen more regionally. Genotype 1 is the most prevalent among the isolates discovered in nearly all regions worldwide. Genotypes 2 and 4 primarily affect individuals in Japan and Taiwan, potentially linked to a less severe form of the disease. Genotype 3 is predominantly found in the Amazon region, where it is associated with notably severe outbreaks of the disease. Genotypes 5 through 8 have been identified in the blood samples of individuals of African descent.²¹

Main Takeaways

(1) Global estimates of HDV prevalence are unreliable or unavailable due to data heterogeneity and testing gaps.

(2) More surveillance data at the country level are needed.

Screening, testing, and diagnosis of HDV

Screening for HDV infection among chronic HBV patients is inconsistent in clinical practice.³ Data from the US Veterans Affairs Medical System indicated that only 8.5% of patients with a positive HBsAg were tested for HDV.²³ The expert panel suggested inconsistencies may be due to differences in screening guidelines (Table 1).

Table 1.

Current guidelines for screening for HDV.

Guideline	Recommendation
EASL, 2023²⁴	“Screening for anti-HDV antibodies should be performed with a validated assay at least once in all HBsAg-positive individuals”
Similar	Re-testing for anti-HDV antibodies should be performed in HBsAg-positive individuals whenever clinically indicated (e.g., in case of aminotransferase flares, or acute decompensation of chronic liver disease), and may be performed yearly in those remaining at risk of infection
DGVS, 2023²⁵
APASL, 2016²⁶	“Other causes of chronic liver disease should be systematically looked for, including co-infections with HDV, HCV, and/or HIV”
AASLD, 2018²⁷	Screen all HBsAg-positive patients at risk for HDV, including those
	1. Born in regions with reported HDV endemicity
	2. Having injected drugs
	3. Men who have sex with men
	4. Diagnosed with HCV or HIV
	5. Having multiple sexual partners or who have any history of sexually-transmitted diseases
	6. With elevated ALT or AST and low HBV-DNA

ALT: alanine aminotransferase; AST: aspartate aminotransferase; DNA: deoxyribonucleic acid; HBV: hepatitis B virus; HCV: hepatitis C virus; HDV: hepatitis D virus; HIV: human immunodeficiency virus.

As shown in Figure 2, Persons Who Inject Drugs (PWIDs) are at the highest risk for developing HDV in high- to middle-income countries.²¹ Moreover, there are dynamic trends in the incidence of HDV within specific high-risk groups. For instance, the prevalence is increasing among men who have sex with men, often linked to drug use during sexual encounters, referred to as “slamming.”²⁸

Figure 2.

Populations at high-risk for HDV infection in middle- to high-income countries. HBV: hepatitis B virus; HCC: hepatocellular carcinoma; HCV: hepatitis C virus; HDV: hepatitis D virus.

Diagnosing HDV begins with screening for HDV antibodies (anti-HDV), using enzyme-linked immunosorbent assays (ELISA). Over 90% of acute HDV infections yield positive anti-HDV within 2 months.²⁹ However, a positive anti-HDV test doesn’t imply immunity.³⁰ According to the expert panelists, anti-HDV-IgG, which develops over several weeks after acute infection and persists with chronic HDV infection, is the preferred screening test over anti-HDV-IgM, which is primarily detectable in serum 2–4 weeks after initial exposure and rarely used in clinical practice, due to its availability and accuracy. Moreover, low titer anti-HDV-IgM is present in some chronically coinfected patients, which is associated with increased disease activity.³¹ Anti-commercial ELISA assays have restricted availability, particularly in the US. Anti-HDV tests, including point-of-care (POC) tests, are working across all genotypes.³²

Main Takeaways

(1) The first step in a diagnosis or screening pathway is total anti-HDV-IgG; in the context of acute hepatitis, anti-HDV-IgM can be requested.

(2) Anti-HDV-IgM is primarily detectable in serum 2–4 weeks after initial exposure and is rarely used in clinical practice.

(3) Anti-HDV-IgG develops several weeks after acute infection and persists with chronic HDV infection.

Patients with anti-HDV + should be tested for quantitative HDV-RNA to confirm active infection.^3,7

Serum HDV-RNA can be detected using qualitative and quantitative reverse transcription-polymerase (RT-PCR) assays; quantitative is preferred.²⁹ HDV-RNA tests face limitations due to lack of standardization, varying characteristics, and limited availability. Many rely on in-house protocols without proper controls or genotype coverage, raising concerns of false negatives.^7,33 Standardized HDV-RNA assays are needed for validation across laboratories. A recent review by Wedemeyer et al. addressed the challenges in achieving accurate pan-genotype HDV-RNA quantification, highlighted its critical role in drug development and patient monitoring, and summarized key features of available HDV-RNA assays.³⁴ Clinicians should always be aware of the limitations of PCR assays as the variability in sensitivity and the inconsistent documentation of negative results obtained from various laboratories pose challenges in the interpretation of HDV-RNA data.

An optimized assay has been suggested using consensus sequences for amplification and the World Health Organization (WHO) International Standards.^29,35,36 The new commercial assays perform well in detecting and quantifying HDV load across genotypes.³⁶ While data on the real-world performance of commercial assays is sparse, notable examples include the RoboGene HDV RNA Quantification Kit 2.0, Diapro HDV Quantitation Real-Time PCR kit, and EurobioPlex HDV kit.³⁴

According to advisors, patients with HDV should undergo testing for HCV and HIV due to shared transmission routes.³⁰ Non-invasive fibrosis scores may be useful

Non-invasive fibrosis markers are valuable for assessment in HBV and HCV, but their role in HBV/HDV coinfection is so far underexplored, lacking defined cirrhosis criteria.^37,38 Delta-4 fibrosis score (D4FS) for chronic HDV needs further validation for cirrhosis identification.³⁹ Transient elastography (TE, FibroScan) appears promising, particularly for cirrhosis exclusion, and unpublished data from AASLD 2024 suggests that a cutoff of <7 kPa is associated with reduced risk of progression.^40,41 Two larger real-world studies have now provided promising data and initial suggestions for cut-off values to exclude cirrhosis and confirm severe fibrosis or cirrhosis.^42,43 The results show a better diagnostic accuracy of TE in detecting cirrhosis compared to APRI, FIB-4, and D4FS.⁴³ Recent studies have also evaluated performance of 3 plasma-based tests for HDV-RNA quantification: Robogene 2.0 HDV-RNA Quantification Kit 2.0 (Roboscreen GmbH; LOD 6 IU/mL on 7500 Fast Real-Time PCR System [Applied Byosystem]), EurobioPlex HDV PCR quantitative (Eurobio Scientific, LOD 100 IU/m) on CFX 96^TM real-time PCR detection system [Bio-Rad]), and AltoStar HDV RT-PCR RUO Kit 1.5 (Altona Diagnostics, estimated LOD <10 IU/mL) on the AltoStar®AM16.⁴⁴ Findings indicated variation in assay quantification over time, suggesting a need for additional research to further improve quantification and establish appropriate cut-offs. Overall, the amount of HDV data is still limited compared to HBV or HCV, so larger studies or pooled analyses are needed for confirmation.

Liver biopsies remain the gold standard for grading and staging.³⁸

Liver histology in patients with HDV characteristically features more intense inflammation than in HBV or HCV and can even mimic autoimmune hepatitis (AIH). Rare reports even indicate disease exacerbation with interferon alfa. Interestingly, some reports describing an improvement in AIH features with bulevirtide^45,46 Fatty liver may also be present.^40,47,48

Other testing/modalities are employed, but limitations restrict their use in clinical practice

Serum HDAg should not be tested (or looked for) as it is present most of the time in the form of immune complexes, and it escapes detection with commercial ELISA assays³⁰ The United States Centers for Disease Control and Prevention is offering HDV genotype testing using a high quality assay system. However, there are no guidelines at present that recommend varying approaches to management based upon HDV genotype, including recommendations on antiviral therapy. This is because there are no well documented differential responses of different HDV genotypes to antiviral treatments. Anti-HDV-IgM indicates ongoing infection/replication, but its clinical use is rare. In cases that HDV-RNA testing is not available, anti-HDV-IgM might be considered as an alternative surrogate test when HDV is suspected.³⁰

Simplifying HDV testing

Frequent clinic and lab visits complicate patient follow-up for diagnosis.⁴⁹ Point-of-care HDV-RNA and reflex testing, proven effective for other viruses, offer immediate results and avoid return visits.⁴⁹ Lempp et al.³² measured anti-HDV with 94.6% sensitivity and 100% specificity using an HDV rapid test. Reflex testing includes automatic anti-HDV screening for all HBsAg-positive cases.⁴⁹ Double-reflex testing involves testing for HDV-RNA if anti-HDV is positive, as per the expert panel.^50,51

Current guidelines

HDV screening rates among HBV-positive patients are universally low.^23,52 In the Veterans Affairs Medical System, less than 8% of HBsAg-positive patients and only 19% meeting “high-risk” criteria were tested for HDV.²³ Similarly, a US Midwest study found just 12% of HBsAg-positive patients were screened for anti-HDV.⁵² Screening rates are even lower in low-income and lower-middle-income countries.¹⁸ The main takeaway box summarizes recommendations for screening.

Main Takeaways

(1) HBsAg-positive patients should be tested at least once with total anti-HDV.

(2) Regardless of previous HDV testing, HBsAg-positive patients with worsening conditions (e.g., elevated ALT levels or hepatic synthetic dysfunction or ongoing risk factors) should be retested using anti-HDV.

(3) Patients who test positive for anti-HDV should be immediately tested for quantitative HDV-RNA PCR using the WHO reference standard (reflex testing, if available); if qHDV-RNA is positive, patients should receive care for both HBV and HDV.

(4) Family testing of household contacts or sexual partners of patients with HDV should occur for HBV, and if positive, for HDV.

Endpoints in clinical trials

Surrogate markers as clinical endpoints in HDV infection aren’t well defined.⁵³ While undetectable HDV-RNA in serum 6 months after the end of therapy is widely accepted as a marker of treatment efficacy, it is not an ideal clinical trial endpoint for chronic HDV due to the risk of late relapses, which have occurred 5–8 years after successful PEG-IFN therapy.^53,54 This limitation is partly attributed to the lower sensitivity of HDV-RNA assays used in earlier studies, which may have failed to detect residual viral replication.^34,55 More data are necessary to validate this endpoint, not only for its broader application but also for therapies such as bulevirtide, to ensure consistent and reliable assessment of treatment outcomes.

An expert panel suggested decreased HDV-RNA of ≥2 log at the end of treatment (combined with ALT) as a surrogate marker for the efficacy of antiviral therapy and regulatory approval.^53,56 Larger, longer term studies are needed for comprehensive evaluation of patient-reported outcomes in chronic HDV (Table 2).

Table 2.

Primary, secondary, and exploratory endpoints for HBV/HDV coinfection.⁵³

Treatment goals	Parameter	Readout
Clinical trial endpoints
Virologic efficacy during treatment	Relative HDV RNA decline during treatment compared to baseline levels	HDV-RNA (IU/ml) with a validated HDV-RNA assay with sufficient sensitivity
Virologic efficacy off treatment	HDV-RNA suppression/decline 24 weeks off-treatment and during further long-term follow-up	HDV-RNA (IU/ml) with a validated HDV RNA assay with sufficient sensitivity
Serological efficacy	HBsAg levels (log declines and loss) at end of and off treatment	Validated quantitative HBsAg (IU/ml) and anti-HBs assay (IU/L)
Biochemical efficacy (1)	ALT normalization at the end of treatment and off treatment	Validated assays (IU/L)
Biochemical efficacy (2)	Relative ALT declines during treatment and off treatment	Validated assays (IU/L)
Combined virologic and biochemical response – EOT	HDV-RNA decline of 2log (or PCR negativity if baseline viral loads is < 100 IU/ml) in combination with ALT normalization at EOT	HDV-RNA (IU/ml) with a validated HDV-RNA assay with sufficient sensitivity. ALT (IU/L) with standard biochemical assays
Combined virologic and biochemical response – off treatment	HDV-RNA decline of 2log (or PCR negativity if baseline viral loads is < 100 IU/ml) in combination with ALT normalization at 24 weeks off treatment and further during long-term follow up	HDV-RNA (IU/ml) with a validated HDV-RNA assay with sufficient sensitivity. ALT (IU/L) with standard biochemical assays
Histological efficacy – grading	Improvement of HAI of at least 2 points	Total Ishak inflammation score (A + B + C+D); 0-18 points
Histological efficacy – staging	No worsening of fibrosis scores	Ishak score (0–6 points)
Safety – drug-specific AEs	AEs and SAEs	Severity and relation of study drug
Safety – disease specific AEs	HBV and HDV reactivation	HBV-DNA, HDV-RNA, ALT and other liver function parameters
ProQOLs	Quality of life during and after end of therapy	EQ5, SF-36
Exploratory endpoints
Liver stiffness	Liver elastography	Fibroscan, ARFI
Serum biomarkers for inflammation and fibrosis	Established scores (e.g., APRI, FIB4, delta fibrosis score) novel parameters	Serum-/plasma tests
Intrahepatic virologic response (HDV and HBV)	Intrahepatic HDV-RNA, HDAg staining, HBV-DNA, HBV-RNA, HBV cccDNA	Virologic assays
Immune responses	HDV-specific T cells, HBV-specific T cells, NK cell frequency and function, soluble inflammatory mediators	T Cell assays, flow cytometry, bead-arrays

AEs: adverse events; AFRI: acoustic radiation force impulse; ALT: alanine aminotransferase; APRI: asparate aminotransferase to platelet radio index; cccDNA: covalently closed circular DNA; EOT: end of treatment; FIB4: fibrosis-4 score; HBV: hepatitis B virus; HBsAg: hepatitis B virus surface antigen; HDV: hepatitis D virus; ProQOLs: professional quality of life scales; SAEs: serious AEs.

Main Takeaways

(1) Undetectable HDV-RNA in serum 6 months after end of therapy is considered an important endpoint, but it requires further validation as late relapses have occurred up to 5–8 years after successful therapy.

(2) Success of finite treatment endpoints:

• HBsAg loss (ideal). The timing of assessment varies according to treatment strategy.

• HDV-RNA below the limit of quantification at 24 weeks off-treatment (there should be a commitment to long-term follow-up for a minimum of 5 years to assess durability of this endpoint with annual HDV-RNA quantitative testing).

• ALT normalization (desired). The timing of assessment varies according to treatment strategy.

• Improvement of necroinflammation and fibrosis.

(3) Goals of long-term treatment:

• ≥2 log reduction in HDV-RNA (but still detectable) combined with normalization of ALT level at 48 weeks on-treatment and during the follow-up on-treatment.

• Undetectable HDV-RNA and normal ALT at the EOT in case of finite therapy.

• The optimal duration of maintenance therapy is currently unknown.

Treatments

Treatment options for chronic HDV are limited. Standard and pegIFNα therapies have been extensively employed against HDV over the past few decades and are approved for HDV treatment in some countries, including Canada⁵⁷ (Table 3). Identifying which patients will respond to pegIFNα is essential, especially since new treatments are not expected soon, especially in countries where HDV is endemic. Bulevirtide has been available since 2020 in some countries (conditional approval in 2020, full approval in 2023 by EMA). Patients with mild disease don’t need immediate therapy and can wait for the availability of new treatments.

Table 3.

HDV treatment guidelines.

Treatment	Guideline recommendations
PEG-IFNα	All patients should be evaluated for PEG-IFNα (EASL, DGVS, AASLD, APASL).^24,26,27,58
	Dose and duration: 120–180 µg once weekly for 48 weeks (EASL, DGVS, AASLD).^24,25,27 12–18 months is suggested by APASL guideline.²⁶
	Prolongation of therapy may be considered if HBsAg declines with good tolerability and compliance with the goal of HBsAg loss (EASL, DGVS).^24,25
	Therapy with PEG-IFNα should not be performed in the presence of decompensated liver disease (DGVS).²⁵
Bulevirtide (BLV)	All patients should be evaluated for BLV (EASL, DGVS).^24,25
	Dose and duration: 2 mg once daily. Treatment duration is not defined.^24,25
	BLV therapy should be continued as long as clinical benefit is evident.
	If decompensation occurs during BLV therapy, therapy should be continued (DGVS).²⁵
PEG-IFNα/BLV	The combination of PEG-IFNα and BLV may be considered in patients without PEG-IFNα intolerance or contraindications (EASL, DGVS).^24,25
NAs therapy	NAs have no direct antiviral efficacy against HDV; however, NAs should be given to patients with compensated cirrhosis and detectable HBV-DNA (EASL, DGVS).^24,25
	NAs should be given in patients without cirrhosis if HBV-DNA levels are higher than 2000 IU/ml (EASL).²⁴
	If HBV-DNA levels are elevated, concurrent therapy with NA using preferred drugs (entecavir, TDF, or TAF) is indicated (AASLD).²⁷
Liver transplant and post-transplant	Patients with decompensated cirrhosis should be evaluated for liver transplantation (EASL, DGVS).^24,25
	Patients who have undergone liver transplantation for CHD should receive HBIG combined with a high genetic barrier NA after transplantation (EASL, DGVS).^24,²⁵
	After the early post-transplant period (6 months), HBIG should be continued (EASL).²⁴

AASLD, American Association for the Study of Liver Diseases; APASL, Asian Pacific Association for the Study of the Liver; BLV, Bulevirtide; CHD, chronic hepatitis D; DGVS, Deutsche Gesellschaft für Gastroenterologie Verdauungs-und Stoffwechselkrankheiten; EASL, European Association for the Study of the Liver; HBIG, hepatitis B immune globulin; HBsAg, hepatitis B surface antigen; HBV-DNA, hepatitis B virus deoxyribonucleic acid; HDV, hepatitis D virus; IU/mL, international units per milliliter; NAs, nucleoside/nucleotide analogues; PEG-IFNα, pegylated interferon alpha; TAF, tenofovir alafenamide; TDF, tenofovir disoproxil fumarate; μg, microgram.

PegIFNα

Studies on IFNα include 2 randomized controlled trials and numerous uncontrolled trials, with prospective and retrospective design. In a systematic review of 15 studies, 17–47% of pegIFNα-treated patients showed virologic response at 24 weeks post-treatment.⁵⁹ However, over 50% of those may experience relapse up to 10 years post-treatment as shown by the Hep-Net-International Delta Hepatitis Intervention I (HIDIT I) study.^54,60 PCR assay sensitivity for HDV-RNA detection has changed from >1000 IU/ml to 6 IU/ml over time, impacting result comparability of these trials. Upon re-testing samples with a more sensitive assay, 31% of previously negative samples were found positive, revealing potential misclassification due to suboptimal sensitivity,⁶¹ which helps to explain the late relapse. Despite HDV-RNA recurrence, the extended HIDIT-I trial revealed better outcomes for patients achieving undetectable HDV-RNA at 6 months during post-treatment follow-up.⁵⁴

Extending IFNα treatment to 2 years in most studies doesn’t appear to increase virologic response rates.^62–64 IFNα has been also employed in CHD patients with compensated cirrhosis.²⁴ Recognizing the heterogeneity of HDV and that the PegIFNα mechanism of action is not well understood, additional research is needed to understand factors impacting response.

Bulevirtide (BLV)

Bulevirtide (BLV) has been used as an antiviral drug since its approval by the European Medicines Agency (EMA) in July 2020 (full approval in July 2023) and is therefore a relatively new addition to HDV therapy and treatment.

Based on data from Phase 2 and Phase 3 studies,⁶⁵ BLV is administered subcutaneously at a dose of 2 mg/day for the treatment of compensated CHD. The phase 3 study showed that 48 weeks of BLV therapy decreased HDV replication, normalized ALT levels in ∼50% of patients, and improved quality of life.^66,67 BLV was well tolerated, with frequent reports of increased bile acids due to BLV’s mechanism of action (binding to sodium taurocholate co-transporting polypeptide) and injection site reactions due to subcutaneous injections.⁶⁶ Real-world studies in chronic HDV and compensated cirrhosis confirmed these promising results.^45,68,69 A recent follow-up of the 48-week phase 3 study presented at EASL in June 2023 found BLV to be a safe and well-tolerated monotherapy for CHD up to week 96, with further improvement in virologic and biochemical response observed with longer treatment duration.⁷³ However, the duration of BLV treatment required to achieve complete virologic control without relapse remains uncertain. Recent real-world data confirm that prolonged suppressive therapy with BLV monotherapy has the potential to reduce viral replication in the long term to such an extent that it may be possible to stop therapy even without HBsAg loss.⁷⁰ Randomized clinical studies on the use of BLV in decompensated liver cirrhosis are currently not available. However, patients with decompensated cirrhosis have already been treated in real-world studies⁷¹ and there were no safety concerns, so that therapy is recommended in individual cases after risk/benefit assessment.⁵⁸

Combination of BLV with PegIFNα

IFNα′s immune modulation, antiviral effects on HBV and HDV, and capacity to hinder HDV spread support its combination with BLV, targeting a lasting virological response.^24,72 In the ongoing MYR204 study, patients were randomized to receive 2 or 10 mg BLV with pegIFNα for 48 weeks, then BLV for another 48 weeks, or 10 mg BLV monotherapy for 96 weeks. The primary endpoint was HDV-RNA undetectable at week 24 after EOT, and the secondary endpoints were undetectable HDV-RNA at EOT and change from baseline in liver stiffness at week 24 after EOT. Additional endpoints included ALT normalization and composite response.

The efficacy and safety of BLV in combination with pegIFNα results were presented recently at AASLD from the phase 2b, open-label, randomized, multicenter study (MYR204). The data demonstrated that in combination with PegIFNα, BLV 10 mg demonstrated the highest rates of HDV-RNA undetectability, ALT normalization, and composite response, surpassing both BLV 10 mg monotherapy and PegIFNα monotherapy (primary endpoint).⁷³ Additionally, BLV 2 mg in combination with PegIFNα outperformed BLV 10 mg monotherapy in terms of HDV-RNA undetectability and composite response. Specifically, the combination therapies showed improvements in liver stiffness observed across the BLV-containing treatment groups. HBsAg loss was infrequent but observed in the combination groups. BLV combined with PegIFNα was safe and well-tolerated as the safety profile observed with BLV (10 mg or 2 mg) in combination was similar to pegIFNα alone. Moreover, few Grade 3 treatment-emergent adverse events (TEAEs) and no serious adverse events (SAEs) related to BLV were reported.⁷³ The study also measured the total serum bile acids and found dose-dependent asymptomatic elevations in bile acids in the BLV groups, attributed to the BLV mechanism of action. However, the increase in the bile only occurred early and the mean levels were stable with continued treatment and even reversible upon treatment completion.

De novo combination therapy shows promise, but studies have had small patient numbers and unexplained differences between dose groups. In a small cohort from Vienna, patients who did not achieve further decreases in HDV-RNA after 24 weeks were offered PEG-IFN as an add-on therapy in a response-guided manner.⁷⁴ The optimal duration for BLV treatment, alone or in combination with PEG-IFN, remains undetermined. More studies are needed on combination therapy for CHD to optimize treatment schedules before proposing it as first-line therapy aiming at curing HDV infection. Currently, BLV plus pegIFNα should be considered for compliant patients within clinical protocols.²⁴

Considerations for anti-HBV therapies

Developing HDV antiviral agents is challenging because it uses host enzymes for replication and can replicate independently of HBV. These viral properties make antiviral agents like nucleos(t)ide analogues (NAs) ineffective. Studies showed NAs like famciclovir, lamivudine, entecavir, and adefovir lacked efficacy against HDV either as monotherapy or in combination with IFNα.^75–78

However, tenofovir disoproxil fumarate (TDF) showed improvement (HDV-RNA and fibrosis) in HIV-coinfected HDV patients,⁷⁹ possibly by restoring the immune system in the HIV-infected cohort or inducing interferon lambda.⁸⁰

In summary, there is no evidence of benefit for NAs therapy in chronic HDV.^81,82 Nevertheless, the treatment principles for HBV monoinfections also apply to coinfected HBV/HDV patients, i.e., NA therapy is indicated if HBV DNA is ≥ 2000 IU/mL or if HBV DNA is positive and advanced fibrosis or cirrhosis is present.^24,58

Liver transplantation

Liver transplantation stands as a viable choice for individuals with end-stage liver diseases. Post-transplant, HBsAg and HDV-RNA clearance is pursued through hepatitis B immune globulin (HBIg) and NAs.⁸³ HDV-RNA decreases after liver transplantation,^84,85 while anti-HDV-IgG and HDV total antibodies persist for years, according to experts. Immunohistochemistry analysis of liver biopsies can detect HDAg up to 590 days post-transplant in HDV-induced liver disease, without detectable ongoing HBV replication. This presence of HDAg in post-transplant livers is not associated with detectable HDV-RNA in the patient’s bloodstream, indicating that HDV in the liver may remain dormant but could potentially reactivate if the patient experiences a superinfection with HBV. This underscores the rationale for considering extended prophylaxis with HBIg and with high genetic barrier NA.⁸⁴

If HDV is present in liver cells it may reproduce, but it is unable to spread in the absence of HBV particles, as HDV lacks HBs proteins expression. While reinfection can occur post-transplant, it is a rare event due to continuous HBIg and NA prophylaxis recommended by major guidelines^24,86–88

New therapies against HDV⁸⁹

(i) Preventing Assembly of Mature HDV Particles: Lonafarnib inhibits HDAg prenylation of large delta antigen (L-HDAg), crucial for HBsAg interaction.¹⁷ Phase 2 studies tested different lonafarnib dosing regimens, alone or with pegIFNα or ritonavir. In LOWR HDV-1,⁹⁰ higher lonafarnib doses lowered HDV viral load, yet triggered more gastrointestinal (GI) events. Adding pegIFNα or ritonavir improved antiviral responses and reduced events compared to lonafarnib alone.⁹⁰ In LOWR-2 study, combining low-dose lonafarnib, ritonavir, and pegIFNα achieved maximum efficacy; again, high-dose lonafarnib resulted in more GI events.⁵⁶ The ongoing D-LIVR phase 3 study investigates lonafarnib (LNF) + ritonavir (RTV) ± pegIFNα in chronic HDV patients on anti-HBV nucleos(t)ide maintenance therapy.⁹¹ According to the 48-week data, both LNF-based regimens, (LNF + RTV, 12.6%) and (LNF + RTV + pegIFNα 24.2%) demonstrated success in meeting the primary efficacy endpoint (the composite virologic and biochemical response rate at end-of-treatment in patients who receive LNF 50 mg/RTV 100 mg BID), outperforming the placebo (2.6%). Furthermore, it is noteworthy that the combination arm showed statistically significant histological improvement.⁹² The 72-week follow-up data, presented in the 2023 EASL meeting, revealed that the combination was still showing consistent endpoint response and the treatment was well tolerated in both arms.

(ii) Preventing Export of HDV Particles: Nucleic acid polymers (NAPs) hinder HBsAg secretion, which in turn can result in blocking HDV secretion from infected cells.⁹³ Phase 2 studies tested NAPs with pegIFNα via infusion in a few CHB/CHD cases, showing off-treatment HBsAg clearance after 63 weeks in several patients.⁹⁴ Off-treatment ALT flares are a potential issue. Several case reports from the Compassionate Use Program show promising results in patients who have failed PEG-IFN and/or BLV therapy.⁹⁵ Further research is required.

(iii) Use of PegIFN-lambda: PegIFNλ is expected to have similar efficacy to pegIFNα but fewer side effects.⁹⁶ A phase 2 study has reported treatment of chronic HDV with PegIFNλ for 48 weeks, which led to a durable virologic response and was associated with a favorable side effect profile.⁹⁷ The phase 3 study investigating the use of 48-week treatment with pegIFNλ has been stopped due to safety concerns because some patients experienced hepatic decompensation.⁹⁸

(iv) Monoclonal antibodies against HBsAg with neutralizing activity, RNA interfering drugs (ASO, siRNA) entered the clinical evaluation, but additional research is needed to validate their use in larger trials and real-world clinical settings.⁹⁹ The Supplemental Table includes additional detail regarding emerging HDV therapies.

Main Takeaways

(1) Patients with mild disease don’t require immediate therapy and may await new treatments’ availability.

(2) PegIFNα for 48 weeks is an option for finite therapy. Treatment beyond 48 weeks generally doesn’t increase overall response rates but some patients benefit from prolonged pegIFNα treatment beyond 1 year.^100,101 Long-term treatment, 5 years follow-up, indicated that liver-related clinical events were uncommon and less likely to occur in patients who experienced virological responses to PEG-IFNα treatment.¹⁰² Reduced dosage with prolonged treatment has been described in case reposts and case series.

(3) Predicting who will respond to pegIFNα is crucial, as new treatments will not become available soon, particularly in HDV endemic countries.

(4) BLV has received approval in Europe by the EMA and represents a breakthrough in the treatment of CHD. However, it is not yet available in other parts of the world, and the treatment duration has not yet been determined

(5) Defining which patients benefit from the combination of pegIFNα plus BLV (de novo, add-on, or sequential) is important. Patients with inadequate response to BLV may benefit from add-on pegIFNα treatment.

(6) HBV NAs are recommended when HBV-DNA ≥2000 IU/mL or in cirrhotic patients with advanced fibrosis or cirrhosis with any detectable HBV-DNA.

Post-treatment and monitoring

Follow-up after HDV treatment varies depending on the treatment regimen and outcomes. Patients treated with pegIFNα with detectable HDV-RNA at EOT may experience subsequent clearance within 24 weeks,⁷⁵ and on the other hand, late relapses have been reported.⁵⁵ Therefore, the advisors recommended a long follow-up period, even in noncirrhotic cases, considering re-treatment or a novel therapy if viremic. Re-treatment urgency might be less with normal ALT; however, clinicians need more data on response maintenance and long-term outcomes, such as fibrosis progression and clinical complications, according to the panel discussion. Noninvasive fibrosis monitoring, such as transient elastography, helps for ongoing management.⁴⁰ Patients with CHD failing treatment response should be monitored at regular intervals for progressive fibrosis which, if found, can lead to efforts to employ novel therapies.¹⁰³

The increased risk of HCC in patients with HDV appears to be related in part to the incremental risk of cirrhosis.¹⁰⁴ The unclear HCC risk in non-cirrhotic HDV patients might prompt broader screening and surveillance. Consistently, guidelines suggest HCC screening and surveillance for all HDV patients. Additionally, incremental HCC risk exists in HIV/HBV/HDV co-infected patients, warranting screening.⁵

Conclusions

Progress has been achieved in addressing HDV, marked by approval and improved access to bulevirtide and updated EASL guidelines in 2023. Further investment and information are crucial to enhance awareness, screening, and the long-term efficacy and safety of therapies. Following the recommendations of clinical experts can lead to improved detection and early intervention of patients with HDV.

Supplemental Material

Supplemental Material - Best practices for screening, testing, diagnosing, and treating patients with hepatitis D (delta) virus based on global expert review and recent guidelines

Supplemental Material for Best practices for screening, testing, diagnosing, and treating patients with hepatitis D (delta) virus based on global expert review and recent guidelines by Markus Cornberg, Fabien Zoulim, Robert Gish, Ira M. Jacobson, Tatyana Kushner, Pietro Lampertico, Mario Rizzetto, Cihan Yurdaydin and Michael Manns in Antiviral Therapy

Ethical considerations

Due to the nature of the review article, ethical approval was not required.

Footnotes

Acknowledgements

The authors would like to thank Ellen Whipple, The Write Source, and Guanine Medical Inc. for medical writing support and Kelly Schrank for editorial support.

ORCID iDs

Robert Gish

Michael Manns

Author contributions

All authors contributed equally to the writing of the manuscript. Prof Dr Manns provided author coordination and editorial support.

Funding

The authors disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: Financial support was provided by Gilead Sciences.

Declaration of conflicting interests

The authors declared the following potential conflicts of interest with respect to the research, authorship, and/or publication of this article: MC: Abbvie – lecturer, consulting, travel support; Gilead Science – lecturer, consulting; Falk Foundation – lecturer; Merck (MSD) – consulting, lecturer; AiCuris – consulting; GlaxoSmithKline (GSK) – lecturer, consulting; Novartis – consulting; Roche – consulting; AstraZeneca – consulting. FZ: lecturer and/or consulting: Aligos, Assembly, AusperBio, Blue Jay, Gilead, GlaxoSmithKline (GSK), nChroma, Precision Biosciences, Vir Bio; research grants: Assembly, AusperBio, Blue Jay, ImCheck. RGG – Grants: Gilead, Consultant/Advisor: Abacus, Abbott, AbbVie, Albireo, Aligos, Altimunne, Antios, Arrowhead, AstraZeneca, Audentes Therapeutics, Corcept, Dynavax, Effectus, Eiger, Eisai, Enyo, Genentech, Genlantis, Gerson Lehrman Group, Gilead Sciences, GlaxoSmithKline, Helios, HepaTX, HepQuant, Intercept, Janssen, JBS Science, Kinnate Bio, Merck, Precision BioSciences, Pfizer, Seres Therapeutics, Topography Health, Tune Therapeutics, Venatorx, Virion, Fibronostics, Fujifilm/Wako, Perspectum, Quest, Sonic Incytes; Scientific or Clinical Advisory Boards: AbbVie, Dynavax, Enyo, Genentech, Genlantis, Gilead, Helios, HepaTX, HepQuant, Intercept, Janssen, Merck, Pfizer, Prodigy; Clinical Trials Alliance: Topography Health; Chair Clinical Advisory Board: Prodigy; Data Safety Monitoring Board: Arrowhead, CymaBay Therapeutics, Durect, Kezar Life Sciences, Sagimet, Takeda; Speaker: AbbVie, AstraZeneca, BMS, Diasorin, Eisai, Genentech, Gilead Sciences Inc., Intercept, Mallinckrodt, VBI Vaccines; Minor stock shareholder: RiboSciences, CoCrystal. IJ – Consulting: Aligos, AusperBio, Barinthus, Gilead, Moderna, Roche, Vir Bio; Data Monitoring Committee: GSK, Precision Biosciences; Research funding: Assembly, Ausperbio, Bluejay, GSK, Vir Bio. TK: Gilead, Abbvie, Mirum, Ipsen, Madrigal, Eiger, GSK – Advisory; Gilead, Mirium, Abbvie – Research support. PL: Advisory Board/Speaker Bureau for Roche Pharma/Diagnostics, Gilead Sciences, Gsk, Abbvie, Janssen, Myr, Eiger, Antios, Aligos, Vir, Grifols, Altona, Roboscreen. MR: Lecturer – Gilead. CY: lecturer and/or consulting: Gilead, Roche, Eiger. MM: Abbvie – lecturer, travel support; Assembly Bio – consulting; Bristol Myers Squibb – consulting, lecturer, travel support; Falk Pharma – consulting, lecturer, travel support, monitoring board/data analysis; Gilead – consulting, lecturer, travel support, monitoring board/data analysis; Merck – principal investigator, grant, consulting, lecturer, travel support, monitoring board/data analysis; GSK – consulting.

Supplemental Material

Supplemental material for this article is available online.

Appendix

References

Rizzetto

Canese

Aricò

, et al. Immunofluorescence detection of new antigen-antibody system (delta/anti-delta) associated to hepatitis B virus in liver and in serum of HBsAg carriers. Gut 1977; 18(12): 997–1003.

Wang

Choo

Weiner

, et al. Structure, sequence and expression of the hepatitis delta (delta) viral genome. Nature 1986; 323(6088): 508–514.

Lempp

Urban

. Hepatitis delta virus: insights into a peculiar pathogen and novel treatment options. Nat Rev Gastroenterol Hepatol 2016; 13(10): 580–589.

Hughes

Wedemeyer

Harrison

. Hepatitis delta virus. The Lancet 2011; 378(9785): 73–85.

Alfaiate

Clément

Gomes

, et al. Chronic hepatitis D and hepatocellular carcinoma: a systematic review and meta-analysis of observational studies. J Hepatol 2020; 73(3): 533–539.

Kapuria

Ben Yakov

Koh

, et al. Spontaneous clearance of chronic delta hepatitis. Hepatology (Baltim, Md) 2020; 71(5): 1873–1875.

Wedemeyer

. Hepatitis D revival. Liver Int 2011; 31: 140–144.

Hayashi

Takeshita

Hutin

YJF

, et al.

The global hepatitis delta virus (HDV) epidemic: what gaps to address in order to mount a public health response?

Arch Public Health 2021; 79(1): 180.

Roulot

Brichler

Layese

, et al. Origin, HDV genotype and persistent viremia determine outcome and treatment response in patients with chronic hepatitis delta. J Hepatol 2020; 73(5): 1046–1062.

10.

Lucifora

Alfaiate

Pons

, et al. Hepatitis D virus interferes with hepatitis B virus RNA production via interferon-dependent and -independent mechanisms. J Hepatol 2023; 78(5): 958–970.

11.

Béguelin

Moradpour

Sahli

, et al. Hepatitis delta-associated mortality in HIV/HBV-coinfected patients. J Hepatol 2017; 66(2): 297–303.

12.

Kucirka

Farzadegan

Feld

, et al. Prevalence, correlates, and viral dynamics of hepatitis delta among injection drug users. J Infect Dis 2010; 202(6): 845–852.

13.

Wang

Shen

, et al. The prevalence of HDV among HBsAg-positive populations with and without HIV-1 in China. Int J Infect Dis 2024; 140: 70–77.

14.

Chen

Oidovsambuu

Liu

, et al. A novel quantitative microarray antibody capture assay identifies an extremely high hepatitis delta virus prevalence among hepatitis B virus–infected mongolians. Hepatology (Baltim, Md) 2017; 66(6): 1739–1749.

15.

Toy

Ahishali

Yurdaydın

. Hepatitis delta virus epidemiology in the industrialized world. AIDS Rev 2020; 22(4): 203–212.

16.

Polaris Observatory Collaborators . Global prevalence, treatment, and prevention of hepatitis B virus infection in 2016: a modelling study. Lancet Gastroenterol Hepatol 2018; 3(6): 383–403.

17.

Rizzetto

Hamid

Negro

. The changing context of hepatitis D. J Hepatol 2021; 74(5): 1200–1211.

18.

Stockdale

Chaponda

Beloukas

, et al. Prevalence of hepatitis D virus infection in sub-Saharan Africa: a systematic review and meta-analysis. Lancet Global Health 2017; 5(10): e992–e1003.

19.

Miao

Zhang

, et al. Estimating the global prevalence, disease progression, and clinical outcome of hepatitis delta virus infection. J Infect Dis 2020; 221(10): 1677–1687.

20.

Chen

Shen

, et al. Prevalence and burden of hepatitis D virus infection in the global population: a systematic review and meta-analysis. Gut 2019; 68(3): 512–521.

21.

Stockdale

Kreuels

Henrion

MYR

, et al. The global prevalence of hepatitis D virus infection: systematic review and meta-analysis. J Hepatol 2020; 73(3): 523–532.

22.

Polaris Observatory Collaborators . Adjusted estimate of the prevalence of hepatitis delta virus in 25 countries and territories. J Hepatol 80: 232–242.

23.

Kushner

Serper

Kaplan

. Delta hepatitis within the Veterans Affairs medical system in the United States: prevalence, risk factors, and outcomes. J Hepatol 2015; 63(3): 586–592.

24.

Brunetto

Ricco

Negro

. EASL clinical practice guidelines on hepatitis delta virus. J Hepatol 2023; 79(2): 433–460.

25.

Sandmann

Berg

Deterding

, et al. Addendum “Antivirale Therapie der chronischen Hepatitis-D-Virusinfektion” zur S3-Leitlinie “Prophylaxe, Diagnostik und Therapie der Hepatitis-B-Virusinfektion” der Deutschen Gesellschaft für Gastroenterologie, Verdauungs- und Stoffwechselkrankheiten (DGVS). Z Gastroenterol 2023; 61(12): 1635–1653.

26.

Sarin

Kumar

Lau

, et al. Asian-Pacific clinical practice guidelines on the management of hepatitis B: a 2015 update. Hepatol Int 2016; 10(1): 1–98.

27.

Terrault

Lok

ASF

McMahon

, et al. Update on prevention, diagnosis, and treatment of chronic hepatitis B: AASLD 2018 hepatitis B guidance. Clin Liver Dis 2018; 12(1): 33–34.

28.

Soriano

de Mendoza

Treviño

, et al. Treatment of hepatitis delta and HIV infection. Liver Int 2023; 43(S1): 108–115.

29.

Shah

Choudhry

Reyes

KJC

, et al. An update on the management of chronic hepatitis D. Gastroenterol Rep 2019; 7(6): 396–402.

30.

Ahn

Gish

. Hepatitis D virus: a call to screening. Gastroenterol Hepatol 2014; 10(10): 647–686.

31.

Wranke

Heidrich

Ernst

, et al. Anti-HDV IgM as a marker of disease activity in hepatitis delta. PLoS One 2014; 9(7): e101002.

32.

Lempp

Roggenbach

Nkongolo

, et al. A rapid point-of-care test for the serodiagnosis of hepatitis delta virus infection. Viruses 2021; 13(12): 2371.

33.

Le Gal

Brichler

Sahli

, et al. First international external quality assessment for hepatitis delta virus RNA quantification in plasma. Hepatology 2016; 64(5): 1483–1494.

34.

Wedemeyer

Leus

Battersby

, et al. HDV RNA Assays: performance characteristics, clinical utility and challenges. Hepatology 2025; 81: 637–650.

35.

Stelzl

Ciesek

Cornberg

, et al. Reliable quantification of plasma HDV RNA is of paramount importance for treatment monitoring: a European multicenter study. J Clin Virol 2021; 142: 104932.

36.

Le Gal

Dziri

Gerber

, et al. Performance characteristics of a new consensus commercial kit for hepatitis D virus RNA viral load quantification. J Clin Microbiol 2017; 55(2): 431–441.

37.

Lutterkort

Wranke

Yurdaydin

, et al. Non-invasive fibrosis score for hepatitis delta. Liver Int 2017; 37(2): 196–204.

38.

Sharma

Khalili

Nguyen

. Non-invasive diagnosis of advanced fibrosis and cirrhosis. World J Gastroenterol 2014; 20(45): 16820–16830.

39.

Surana

Kleiner

, et al. The Delta-4 fibrosis score (D4FS): a novel fibrosis score in chronic hepatitis D. Antivir Res 2020; 174: 104691.

40.

Surana

Takyar

, et al. Vibration-controlled transient elastography for the detection of cirrhosis in chronic hepatitis D infection. J Viral Hepat 2020; 27(4): 428–436.

41.

Huang

, et al. Accuracy of FibroScan in analysis of liver fibrosis in patients with concomitant chronic Hepatitis B and nonalcoholic fatty liver disease. Medicine 2020; 99(23): e20616.

42.

Sandmann

Degasperi

Port

, et al. Liver stiffness measurement as a noninvasive method for the diagnosis of liver cirrhosis in patients with chronic hepatitis D virus infection. Aliment Pharmacol Ther 2024; 59: 752–761. Published online 11 January.

43.

Roulot

Brichler

Layese

, et al. Liver stiffness measurement to detect severe fibrosis and cirrhosis in patients with chronic hepatitis D infection. AASLD The Liver Meeting. 2023. Accessed December 12, 2023. https://www.natap.org/2023/AASLD/AASLD_62.htm

44.

Anolli

Renteria

Degasperi

, et al. Comparison of 3 methods for plasma HDV-RNA quantification in bulevirtide-treated and untreated HDV patients. JHEP Report. 2024; 7(3): 101299. doi:10.1016/j.jhepr.2024.101299.

45.

Loglio

Ferenci

Uceda Renteria

, et al. Safety and effectiveness of up to 3 years’ bulevirtide monotherapy in patients with HDV-related cirrhosis. J Hepatol 2022; 76(2): 464–469.

46.

Gigi

Lagopoulos

Liakos

. Management of autoimmune hepatitis induced by hepatitis delta virus. World J Gastroenterol 2024; 30(8): 799–805.

47.

Enc

Ulasoglu

. A case of autoimmune hepatitis following pegylated interferon treatment of chronic hepatitis delta. North Clin Istanb 2020; 7(4): 407–410.

48.

Sagnelli

Felaco

Filippini

, et al. Influence of HDV infection on clinical, biochemical and histological presentation of HBsAg positive chronic hepatitis. Liver 1989; 9(4): 229–234.

49.

Feld

. Hepatitis C virus diagnostics: the road to simplification. Clin Liver Dis 2018; 12(5): 125–129.

50.

Palom

Rando-Segura

Vico

, et al. Implementation of anti-HDV reflex testing among HBsAg-positive individuals increases testing for hepatitis D. JHEP Rep 2022; 4(10): 100547.

51.

Analytik

Diagnostic tools for antiviral therapy monitoring: RoboGene product family. Analytik Jena. Accessed October 20, 2023. https://www.analytik-jena.com/fileadmin/import/assets/12560648_Br_RoboGene_Family_en.pdf

52.

Safaie

Razeghi

Rouster

, et al. Hepatitis D diagnostics: utilization and testing in the United States. Virus Res 2018; 250: 114–117.

53.

Yurdaydin

Abbas

Buti

, et al. Treating chronic hepatitis delta: the need for surrogate markers of treatment efficacy. J Hepatol 2019; 70(5): 1008–1015.

54.

Wranke

Hardtke

Heidrich

, et al. Ten-year follow-up of a randomized controlled clinical trial in chronic hepatitis delta. J Viral Hepat 2020; 27(12): 1359–1368.

55.

Bremer

Anastasiou

Hardtke

, et al. Residual low HDV viraemia is associated HDV RNA relapse after PEG-IFNa-based antiviral treatment of hepatitis delta: results from the HIDIT-II study. Liver Int 2021; 41(2): 295–299.

56.

Yurdaydin

Keskin

Yurdcu

, et al. A phase 2 dose-finding study of lonafarnib and ritonavir with or without interferon alpha for chronic delta hepatitis. Hepatology 2022; 75(6): 1551–1565.

57.

Urban

Neumann-Haefelin

Lampertico

. Hepatitis D virus in 2021: virology, immunology and new treatment approaches for a difficult-to-treat disease. Gut 2021; 70(9): 1782–1794.

58.

Sandmann

Berg

Deterding

, et al. Addendum “antivirale therapie der chronischen hepatitis-D-virusinfektion“ zur S3-leitlinie” prophylaxe, diagnostik und therapie der hepatitis-B-virusinfektion“ der Deutschen Gesellschaft für Gastroenterologie, Verdauungs- und Stoffwechselkrankheiten (DGVS). Z Gastroenterol. 2023; 61(12): 1635–1653. doi:10.1055/a-2181-3046.

59.

Sandmann

Wedemeyer

. Interferon-based treatment of chronic hepatitis D. Liver Int 2023; 43(Suppl 1): 69–79.

60.

Heidrich

Yurdaydın

Kabaçam

, et al. Late HDV RNA relapse after peginterferon alpha-based therapy of chronic hepatitis delta. Hepatology (Baltim, Md) 2014; 60(1): 87–97.

61.

Abbas

Khan

Salih

, et al. Interferon alpha for chronic hepatitis D. Cochrane Database Syst Rev 2011; 2011(12): CD006002.

62.

Wedemeyer

Yurdaydin

Hardtke

, et al. Peginterferon alfa-2a plus tenofovir disoproxil fumarate for hepatitis D (HIDIT-II): a randomised, placebo controlled, phase 2 trial. Lancet Infect Dis 2019; 19(3): 275–286.

63.

Gunsar

Akarca

Ersoz

, et al. Two-year interferon therapy with or without ribavirin in chronic delta hepatitis. Antivir Ther 2005; 10(6): 721–726.

64.

Yurdaydin

Bozkaya

Karaaslan

, et al. A pilot study of 2 years of interferon treatment in patients with chronic delta hepatitis. J Viral Hepat 2007; 14(11): 812–816.

65.

Wedemeyer

Aleman

Brunetto

, et al. A phase 3, randomized trial of bulevirtide in chronic hepatitis D. N Engl J Med 2023; 389(1): 22–32.

66.

Wedemeyer

Aleman

Brunetto

, et al. Efficacy and safety of bulevirtide monotherapy given at 2 mg or 10 mg dose level once daily for treatment of chronic hepatitis delta: week 48 primary end point results from a phase 3 randomized, multicenter, parallel design study. J Hepatol 2022; 77: S4–S5.

67.

Buti

Wedemeyer

Aleman

, et al. Treatment with bulevirtide improves patient-reported outcomes in patients with chronic hepatitis delta: an exploratory analysis of a Phase 3 trial at 48 weeks. J Hepatol 2022; 77: S103.

68.

Degasperi

Anolli

Uceda Renteria

, et al. Bulevirtide monotherapy for 48 weeks in patients with HDV-related compensated cirrhosis and clinically significant portal hypertension. J Hepatol 2022; 77(6): 1525–1531.

69.

Lampertico

Roulot

Wedemeyer

. Bulevirtide with or without pegIFNα for patients with compensated chronic hepatitis delta: from clinical trials to real-world studies. J Hepatol 2022; 77(5): 1422–1430.

70.

Anolli

Degasperi

Allweiss

, et al. A 3-year course of bulevirtide monotherapy may cure Hdv infection in cirrhotics. J Hepatol. 2023; 3:S0168-8278(22)03475-4. DOI: 10.1016/j.jhep.2022.12.023. Published online January 3.

71.

Dietz-Fricke

Tacke

Zöllner

, et al. Treating hepatitis D with bulevirtide – real-world experience from 114 patients. JHEP Rep 2023; 5(4): 100686.

72.

Zhang

Lempp

, et al. Hepatitis D virus-induced interferon response and administered interferons control cell division-mediated virus spread. J Hepatol 2022; 77(4): 957–966.

73.

Asselah

Chulanov

Lampertico

, et al. Bulevirtide combined with pegylated interferon for chronic hepatitis D. N Engl J Med 2024; 391(2): 133–143.

74.

Jachs

Schwarz

Panzer

, et al. Response‐guided long‐term treatment of chronic hepatitis D patients with bulevirtide—results of a “real world” study. Aliment Pharmacol Ther 2022; 56(1): 144–154.

75.

Wedemeyer

Yurdaydìn

Dalekos

, et al. Peginterferon plus adefovir versus either drug alone for hepatitis delta. N Engl J Med 2011; 364(4): 322–331.

76.

Niro

Ciancio

Tillman

, et al. Lamivudine therapy in chronic delta hepatitis: a multicentre randomized-controlled pilot study. Aliment Pharmacol Ther 2005; 22(3): 227–232.

77.

Kabaçam

Onder

Yakut

, et al. Entecavir treatment of chronic hepatitis D. Clin Infect Dis 2012; 55(5): 645–650.

78.

Yurdaydin

Bozkaya

Gürel

, et al. Famciclovir treatment of chronic delta hepatitis. J Hepatol 2002; 37(2): 266–271.

79.

Soriano

Vispo

Sierra-Enguita

, et al. Efficacy of prolonged tenofovir therapy on hepatitis delta in HIV-infected patients. AIDS (London, England) 2014; 28(16): 2389–2394.

80.

Murata

Asano

Matsumoto

, et al. Induction of IFN-λ3 as an additional effect of nucleotide, not nucleoside, analogues: a new potential target for HBV infection. Gut 2018; 67(2): 362–371.

81.

Wranke

Serrano

Heidrich

, et al. Antiviral treatment and liver-related complications in hepatitis delta. Hepatology 2017; 65(2): 414–425.

82.

Kamal

Westman

Falconer

, et al. Long-term study of hepatitis delta virus infection at secondary care Centers: the impact of viremia on liver-related outcomes. Hepatology (Baltim, Md) 2020; 72(4): 1177–1190.

83.

Danziger-Isakov

Kumar

AST ID Community of Practice . Vaccination of solid organ transplant candidates and recipients: guidelines from the American society of transplantation infectious diseases community of practice. Clin Transplant 2019; 33(9): e13563.

84.

Mederacke

Filmann

Yurdaydin

, et al. Rapid early HDV RNA decline in the peripheral blood but prolonged intrahepatic hepatitis delta antigen persistence after liver transplantation. J Hepatol 2012; 56(1): 115–122.

85.

Smedile

Casey

Cote

, et al. Hepatitis D viremia following orthotopic liver transplantation involves a typical HDV virion with a hepatitis B surface antigen envelope. Hepatology (Baltim, Md) 1998; 27(6): 1723–1729.

86.

Kushner

Chan

, et al. Liver transplantation for hepatitis D virus in the United States: a UNOS study on outcomes in the meld era. Transplant Direct 2022; 8(1): e1253.

87.

Adil

Fatih

Volkan

, et al. Hepatitis B virus and hepatitis D virus recurrence in patients undergoing liver transplantation for hepatitis B virus and hepatitis B virus plus hepatitis D virus. Transplant Proc 2016; 48(6): 2119–2123.

88.

Cholongitas

Goulis

Antoniadis

, et al. Nucleos(t)ide analog(s) prophylaxis after hepatitis B immunoglobulin withdrawal against hepatitis B and D recurrence after liver transplantation. Transpl Infect Dis 2016; 18(5): 667–673.

89.

Hepatitis B Foundation . 2023c. Hepatitis Delta Connect [Internet]. Doyeston (PA): Hepatitis B Foundation. Drug watch. Accessed September 8, 2023. https://www.hepb.org/research-and-programs/hepdeltaconnect/drug-watch/

90.

Yurdaydin

Keskin

Kalkan

, et al. Optimizing lonafarnib treatment for the management of chronic delta hepatitis: the LOWR HDV-1 study. Hepatology (Baltim, Md) 2018; 67(4): 1224–1236.

91.

Eiger BioPharmaceuticals . A phase 3, matrix design, partially double-blind, randomized Study of the Efficacy and Safety of 50 Mg lonafarnib/100 Mg ritonavir BID With and without 180 mcg PEG IFN-Alfa-2a for 48 Weeks compared with PEG IFN-Alfa-2a Monotherapy and placebo Treatment in patients chronically infected with hepatitis delta virus being Maintained on anti-HBV nucleos(t)Ide therapy (D-LIVR). clinicaltrials.gov, 2023. https://clinicaltrials.gov/study/NCT03719313 (Accessed 24 August 2023).

92.

Etzion

Hamid

Asselah

, et al. Week 48 results of the phase 3 D-LIVR study, a randomized double-blind, placebo-controlled trial evaluating the safety and efficacy of Lonafarnib-boosted with Ritonavir with or without Peginterferon Alfa in patients with chronic hepatitis delta. J Hepatol 2023; 78: S10.

93.

Fonte

Plissonnier

Michelet

, et al. REP 2139 targets the Hepatitis Delta Virus (HDV) ribonucleoprotein (RNP) and exerts a direct antiviral effect on HDV replication. AASLD The Liver Meeting. 2023. https://aasld.confex.com/aasld/2023/mediafile/Handout/Paper45652/Fonte20et20al20Poster20AASLD20202320END.pdf

94.

Brancaccio

Gaeta

Vitale

, et al. Recent breakthroughs in the treatment of chronic hepatitis Delta. Infez Med 2022; 30(2): 204–210.

95.

Stern

Freitas

Bazinet

, et al. 550 safety and efficacy of rep 2139-MG in association with TDF in patients with chronic hepatitis delta and decompensated cirrhosis. Gastroenterology 2023; 164(6): S.

96.

Donnelly

Kotenko

. Interferon-lambda: a new addition to an old family. J Interferon Cytokine Res 2010; 30(8): 555–564.

97.

Etzion

Hamid

Lurie

, et al. Treatment of chronic hepatitis D with peginterferon lambda-the phase 2 LIMT-1 clinical trial. Hepatology 2023; 77(6): 2093–2103.

98.

Eiger BioPharmaceuticals . Phase 3, randomized, open-label, parallel arm study to evaluate the efficacy and safety of 180 mcg peginterferon lambda-1a (lambda) subcutaneous injection for 48 Weeks in patients with chronic hepatitis delta virus (HDV) infection (LIMT-2). clinicaltrials.gov, 2023. https://clinicaltrials.gov/study/NCT05070364 (Accessed 24 August 2023).

99.

Asselah

Streinu-Cercel

Jucov

, et al. The monoclonal antibody VIR-3434 and siRNA VIR-2218 for the treatment of chronic Hepatitis D Virus: preliminary results from the Phase 2 SOLSTICE trial. AASLD The Liver Meeting. 2023. Accessed February 5, 2024. https://www.natap.org/2023/AASLD/AASLD_61.htm

100.

Heller

Rotman

Koh

, et al. Long-term therapy of chronic delta hepatitis with peginterferon alfa. Aliment Pharmacol Ther 2014; 40(1): 93–104.

101.

Yurdaydin

Keskin

Kalkan

, et al. Interferon treatment duration in patients with chronic delta hepatitis and its effect on the natural course of the disease. J Infect Dis 2018; 217(8): 1184–1192.

102.

Anastasiou

Caruntu

Curescu

, et al. Five‐year follow‐up of 96 weeks peginterferon plus tenofovir disoproxil fumarate in hepatitis D. Liver Int 2024; 44: 139–147.

103.

Kalkan

Yılmaz

Erdoğan

, et al. Non–invasive fibrosis markers for assessment of liver fibrosis in chronic hepatitis delta. J Viral Hepat 2023; 30(5): 406–416.

104.

Bockmann

Grube

Hamed

, et al. High rates of cirrhosis and severe clinical events in patients with HBV/HDV co-infection: longitudinal analysis of a German cohort. BMC Gastroenterol 2020; 20(1): 24.

105.

Botelho-Souza

Alves Vasconcelos

Oliveira Dos Santos

, et al. Hepatitis delta: virological and clinical aspects. Virol J. 2017; 14(1): 177. doi:10.1186/s12985-017-0845-y.

Supplementary Material

Please find the following supplemental material available below.

For Open Access articles published under a Creative Commons License, all supplemental material carries the same license as the article it is associated with.

For non-Open Access articles published, all supplemental material carries a non-exclusive license, and permission requests for re-use of supplemental material or any part of supplemental material shall be sent directly to the copyright owner as specified in the copyright notice associated with the article.

0.00 MB

0.22 MB

Best practices for screening,testing,diagnosing,and treating patients with hepatitis D (delta) virus based on global expert review and recent guidelines

Abstract

Background

Methods

Results

Conclusion

Keywords

Introduction & methods

Results & discussion

Natural history of HDV

Epidemiology of HDV

Screening, testing, and diagnosis of HDV

Patients with anti-HDV + should be tested for quantitative HDV-RNA to confirm active infection.3,7

According to advisors, patients with HDV should undergo testing for HCV and HIV due to shared transmission routes. 30 Non-invasive fibrosis scores may be useful

Liver biopsies remain the gold standard for grading and staging. 38

Other testing/modalities are employed, but limitations restrict their use in clinical practice

Simplifying HDV testing

Current guidelines

Endpoints in clinical trials

Treatments

PegIFNα

Bulevirtide (BLV)

Combination of BLV with PegIFNα

Considerations for anti-HBV therapies

Liver transplantation

New therapies against HDV 89

Post-treatment and monitoring

Conclusions

Supplemental Material

Supplemental Material - Best practices for screening, testing, diagnosing, and treating patients with hepatitis D (delta) virus based on global expert review and recent guidelines

Ethical considerations

Footnotes

Acknowledgements

ORCID iDs

Author contributions

Funding

Declaration of conflicting interests

Supplemental Material

Appendix

References

Supplementary Material

Patients with anti-HDV + should be tested for quantitative HDV-RNA to confirm active infection.^3,7

According to advisors, patients with HDV should undergo testing for HCV and HIV due to shared transmission routes.³⁰ Non-invasive fibrosis scores may be useful

Liver biopsies remain the gold standard for grading and staging.³⁸

New therapies against HDV⁸⁹