Fifty canine lymphomas were classified cytomorphologically using the updated Kiel classification scheme. Aspirates of lymph nodes from dogs with lymphoma were stained using 5 canine-specific antibodies and 3 human-specific antibodies that cross-react with canine lymphocytes. The antibody-stained aspirates were analyzed by flow cytometry. A total of 32 (64%) of the 50 lymphomas were characterized as B-cell origin and 18 (36%) were of T-cell origin. B-cell lymphomas were identified in 12 females and 20 males with a mean age of 8.35 years. T-cell lymphomas were identified in 8 females and 10 males with a mean age of 7.9 years. A minority of the lymphomas were low-grade B-cell and T-cell lymphomas (6/50, 12% and 4/50, 8%, respectively). The most common morphologic types were high-grade centroblastic and unclassifiable plasmacytoid for B- and T-cell lymphomas (18/50, 36% and 7/50, 14%, respectively).
AppelbaumFRSaleGEStorbR: 1984, Phenotyping of canine lymphoma with monoclonal antibodies directed at cell surface antigens: classification, morphology, clinical presentation and response to chemotherapy. Hematol Oncol2:151–168.
2.
CaniattiMRoccabiancaPScanzianiE: 1996, Canine lymphoma: immunocytochemical analysis of fine-needle aspiration biopsy. Vet Pathol33:204–212.
3.
CarterRFValliVE: 1988, Advances in the cytologic diagnosis of canine lymphoma. Semin Vet Med Surg (Small Anim)3:167–175.
4.
CarterRFValliVELumsdenJH: 1986, The cytology, histology and prevalence of cell types in canine lymphoma classified according to the National Cancer Institute Working Formulation. Can J Vet Res50:154–164.
5.
CobboldSMetcalfeS: 1994, Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW). Tissue Antigens43:137–154.
6.
CulmseeKSimonDMischkeR: 2001, Possibilities of flow cytometric analysis for immunophenotypic characterization of canine lymphoma. J Vet Med Ser A47:199–206.
7.
DobsonJMBlackwoodLBMcInnesEF: 2001, Prognostic variables in canine multicentric lymphosarcoma. J Small Anim Pract42:377–384.
8.
FerrerLFondeuilaDRabanalR: 1993, Immunohistochemical detection of CD3 antigen (pan-T marker) in canine lymphomas. J Vet Diagn Invest5:616–620.
9.
Fournel-FleuryCMagnolJPBricaireP: 1997, Cytohistological and immunological classification of canine malignant lymphomas: comparison with human non-Hodgkin's lymphomas. J Comp Pathol117:35–59.
10.
Fournel-FleuryCMagnolJPChabanneL: 1997, Growth fractions in canine non-Hodgkin's lymphomas as determined in situ by the expression of the Ki-67 antigen. J Comp Pathol117:61–72.
11.
Fournel-FleuryCPonceFFelmanP: 2002, Canine T-cell lymphomas: a morphological, immunological, and clinical study of 46 new cases. Vet Pathol39:92–109.
12.
GreenleePGFilippaDAQuimbyFW: 1990, Lymphomas in dog. A morphologic, immunologic and clinical study. Cancer66:480–490.
13.
HagaTYokomoriKNakayamaH: 1988, Canine and feline lymphoid and myeloid tumors encountered in Tokyo. Jpn J Vet Sci50:809–813.
14.
JaffeES: 1999, Hematopathology: integration of morphologic features and biologic markers for diagnosis. Mod Pathol12:109–115.
15.
JaffeESHarrisNLSteinHVardimanJW: 2001, Pathology and genetics of tumours of haematopoietic and lymphoid tissues. In: World health organization classification of tumours, ed. JaffeESHarrisNLSteinHVardimanJW, pp. 41–58. IARC Press, Lyon, France.
16.
JagielskiDLechowskiRHoffmann-JagielskaM: 2002, A retrospective study of the incidence and prognostic factors of multicentric lymphoma in dogs (1998–2000). J Vet Med A Physiol Pathol Clin Med49:419–424.
17.
KellerETMacEwenEGRosenthalRC: 1993, Evaluation of prognostic factors and sequential combination chemotherapy with doxorubicin for canine lymphoma. J Vet Intern Med7:289–295.
18.
LadigesWCKeastMAppebaumFR: 1988, Phenotypic characterization of canine lymphoma, using monoclonal antibodies and a microlymphotoxicity assay. Am J Vet Res49:870–872.
19.
LennertKFellerCA: 1991, Histologie des Lymphomes Malins Non Hodgkiniens Selon la Classification de Kiel Actualisée. Doin, Paris, France.
20.
MagnolJPFournel-FleuryCMarchalT: 1995, Lymphomes malins à moyennes cellules macronucléolées dans l'espèce canine: implication d'une cellule originale de la zone marginale du ganglion lymphatique réactionnel. Bull Acad Nat Med1:51–65.
21.
PileriSAMilaniMFraternali-OrcioniG: 1998, From the R.E.A.L. Classification to the upcoming WHO scheme: a step toward universal categorization of lymphoma entities? Ann Oncol9:607–612.
22.
PonceFMagnolJPMarchalT: 2003, High-grade canine T-cell lymphoma/leukemia with plasmacytoid morphology: a clinical pathological study of nine cases. J Vet Diagn Invest15:330–337.
23.
PriesterWAMcKayFW: 1980, The occurrence of tumors in domestic animals. Natl Cancer Inst Monogr54:166.
24.
RaskinRE: 2001, Lymphoid system. In: Atlas of canine and feline cytology, ed. RaskinREMeyerDJ, pp. 93–134. WB Saunders, Philadelphia, PA.
25.
RotheGSchmitzG: 1996, Consensus protocol for the flow cytometric immunophenotyping of hematopoietic malignancies. Leukemia10:877–895.
26.
RuslanderDAGebhardDHTompkinsMB: 1997, Immunophenotypic characterization of canine lymphoproliferative disorders. In Vivo112:169–172.
27.
Solal-CelignyPBrousseNFerméC: 1997, Lymphomes, lymphomes non Hodgkiniens-maladie de Hodgkin. Frison-Roche, 3rd ed., pp. 527. Aubin Press, Paris, France.
28.
TeskeE: 1994, Canine malignant lymphoma: a review and comparison with human non-Hodgkin's lymphoma. Vet Q164:209–219.
29.
TeskeE: 1999, Canine malignant lymphoma: a review and comparison with human non-Hodgkin's lymphoma. In: Canine cancer, pp. 13–29. Hill's Pet Nutrition Inc. Topeka, KS.
30.
TeskeEWismanPMoorePF: 1994, Histologic classification and immunophenotyping of canine non-Hodgkin's lymphomas: unexpected high frequency of T cell lymphomas with B cell morphology. Exp Hematol22:1179–1187.
31.
The Non-Hodgkin's Lymphoma Classification Project: 1997, A clinical evaluation of the International Lymphoma Study Group classification of non-Hodgkin's lymphoma. Blood89:3909–3918.
32.
ValliVE: 1988, Techniques in veterinary cytopathology. Semin Vet Med Surg (Small Anim)3:85–93.
33.
WithrowSJ: 1996, Why worry about cancer in pet animals? In: Small animal clinical oncology, ed. WithrowSJMcEwenEG, 2nd ed., pp. 1–3. WB Saunders, Philadelphia, PA.
34.
YoungNAAl-SaleemTIEhyaH: 1998, Utilization of fine-needle aspiration cytology and flow cytometry in the diagnosis and subclassification of primary and recurrent lymphoma. Cancer (Cancer Cytopathol)84:252–261.
