Sage Journals: Discover world-class research

Abstract

Previously, we found that mouse TH2.52 cells possess the characteristic of CD5⁺ B1 cells and proliferate in response to lipopolysaccharide (LPS). The effect of LPS on cytokine production by TH2.52 B1 cells was studied. TH2.52 cells constitutively produced a small amount of tumor necrosis factor (TNF)-α and interleukin (IL)-6, and TNF-α and IL-6 production was markedly enhanced by LPS stimulation. Although interferon (IFN)-γ caused the production of various cytokines, such as IL-2, IL-4, IL-6 and TNF-α in TH2.52 cells, LPS did not cause the production of such cytokines. LPS did not induce IFN-β production in TH2.52 cells and TH2.52 cells lacked the expression of several molecules participating in the MyD88-independent pathway in LPS signaling. Defective responsiveness of TH2.52 B1 cells to LPS in cytokine production might be responsible for the failure of IFN-β production due to the lack of molecules participating in the MyD88-independent pathway.

Keywords

CD5+ B cell B1 cell LPS IFN-γ TNF-α

References

Hardy RR , Hayakawa K. B cell development pathways. Annu Rev Immunol 2001; 19: 595—621.

Dono M. , Cerruti G. , Zupo S. The CD5+ B-cell. Int J Biochem Cell Biol 2004; 36: 2105—2111.

Borrello MA , Phipps RP Fibroblast-secreted macrophage colony-stimulating factor is responsible for generation ofbiphenotypic B/macrophage cells from a subset of mouse B lymphocytes . J Immunol 1999; 163: 3605—3611.

Borrello MA , Palis J. , Phipps RP The relationship of CD5+ B lymphocytes to macrophages: insights from normal biphenotypic B/macrophage cells. Int Rev Immunol 2001; 20: 137—155.

Hamano T. , Kim KJ , Leiserson WM et al. Establishment of B cell hybridomas with B cell surface antigens . J Immunol 1982; 129: 1403—1406.

Hamano T. , Asofsky R. Functional studies on B cell hybridomas with B cell surface antigens. I. Effects of anti-immunoglobulin antibodies on proliferation and differentiation. J Immunol 1983; 130: 2027—2032.

Little J. , Alling DW , Asofsky R. Induction of differentiation in a B lymphoma x B lymphocyte hybrid line. II. Intraclonal heterogeneity in growth, secretion of IgM, and cytokine production in response to lipopolysaccharide. J Immunol 1993; 150: 2129—2138.

Koide N. , Sugiyama T. , Kato Y. et al. Mouse B1 cell line responds to lipopolysaccharide via membrane-bound CD14. J Endotoxin Res 2001; 7: 39—43.

Sugiyama T. , Koide N. , Chakravortty D. et al. The expression of membrane-bound CD14 renders mouse B1 cells susceptible to LPS. J Endotoxin Res 2001; 7: 223—226.

10.

Koide N. , Sugiyama T. , Mu MM et al. Gamma interferon-induced nitric oxide production in mouse CD5 + B1-like cell line and its association with apoptotic cell death. Microbiol Immunol 2003; 47: 669—679.

11.

Koide N. , Sugiyama T. , Mori I. et al. Change of mouse CD5(+) B1 cells to a macrophage-like morphology induced by gamma interferon and inhibited by interleukin-4. Clin Diagn Lab Immunol 2002; 9: 1169—1174.

12.

Seideman J. , Peritt D. A novel monoclonal antibody screening method using the Luminex-100 microsphere system. J Immunol Methods 2002; 267: 165—171.

13.

Sugiyama T. , Fujita M. , Koide N. et al. 2-aminopurine inhibits lipopolysaccharide-induced nitric oxide production by preventing IFN-beta production. Microbiol Immunol 2004; 48: 957—963.

14.

Sato N. , Takahashi N. , Suda K. et al. MyD88 but not TRIF is essential for osteoclastogenesis induced by lipopolysaccharide, diacyl lipopeptide, and IL-1alpha. J Exp Med 2004; 200: 601—611.

15.

Koide N. , Ito H. , Mu MM et al. Inhibition of extracellular signal-regulated kinase 1/2 augments nitric oxide production in lipopolysaccharide-stimulated RAW264.7 macrophage cells. FEMS Immunol Med Microbiol 2005; 45: 213—219.

16.

Van der Meide PH , Schellekens H. Cytokines and the immune response. Biotherapy 1996; 8: 243—249.

17.

Takeuchi O. , Hemmi H. , Akira S. Interferon response induced by Toll-like receptor signaling. J Endotoxin Res 2004; 10: 252—256.

Defective responsiveness of CD5+ B1 cells to lipopolysaccharide in cytokine production

Abstract

Keywords

References