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Abstract

Poly(ADP-ribose) metabolism is altered in patients with SLE. In order to localize the defect, the levels of poly(ADP-ribose) polymerase-specific mRNA were measured from dot blots of total RNA from peripheral blood lymphocytes. In this preliminary study, eleven patients with SLE and two with antiphospholipid syndrome were compared to three controls. It was found that the mean levels of specific mRNA were ten fold lower in the PBL from SLE patients compared to controls and no overlap of values was seen between the two groups. No such decrease was seen in the PBL from the patients with antiphospholipid syndrome. It is concluded that the defect in poly(ADP-riboseO polymerase metabolism that is seen in SLE patients occurs at the level of transcription or mRNA turnover.

Keywords

SLE Poly(ADP-ribose) polymerase mRNA

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References

Hayaishi O. , Ueda K. ADP-Ribosylation Reactions. New York, Academic Press, 1982.

Wintersberger U. , Wintersberger E. Poly

ADP-ribossylation - a cellular emergency reaction?

FEBS Lett 1985; 188: 189-91.

Duskatz BW , Irwin J. , Shall S. The effect of inhibition of (ADPribose)n biosynthesis on DNA repair assayed by the nucleoid technique. Eur J Biochem 1981 ; 121: 65-9.

Johnstone AP , Williams GT Role of DNA breaks and ADP-ribosyl transferase activity in eukaryotic differentiation demonstrated in human lymphocytes . Nature 1982; 300: 368-70.

Kawamitsu H. , Hoshino H. , Okada H. , Miwa M. , Momoi H. , Sugmura T. Monoclonal antibodies to poly(adenosine diphosphate ribose) recognize different structures. Biochemistry 1984; 23: 3771-7.

Sibley JT , Braun RP , Lee JS The production of antibodies to DNA in normal mice following immunization with poly(ADP-ribose). Clin Exp Immunol 1986 ; 64: 563-9.

Clayton A-L. , Bernstein RM , Tavassoli M. et al. Measurement of antibody to poly(adensoine diphosphate-ribose): its diagnostic value in systemic lupus erythematosus . Clin Exp Immunol 1984 ; 56: 263-71.

Haug BL , Sibley JT , Lee JS Increased synthesis of poly(ADP-ribose) in isolated liver nuclei from autoimmune NZB/NZW mice. FEBS Lett 1987; 215: 252-6.

Sibley JT , Haug BL , Lee JS Altered metabolism of poly(ADP-ribose) in the peripheral blood lymphocytes of patients with systemic lupus erythematosus. Arthritis Rheum 1989; 32: 1045-9.

10.

Tan EN , Cohen AS , Fries JF et al. The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum 1982; 25: 1271-7.

11.

Sibley JT , Olszynski WO , Decoteau WE , Sundaram MB The incidence and prognosis of central nervous system disease in systemic lupus erythematosus. J Rheumatol 1992; 19: 47-52.

12.

Chomcznski P. , Lesk AM Single step method of RNA isolation by acid guanidinium thiocyanate phenol chloroform extraction. Anal Biochem 1987; 162: 156-9.

13.

Morgan AR , Lee JS , Pulleyblank DE , Murray NL , Evans DH Ethidium fluorescence assays. 1. Physiochemical studies. Nucleic Acids Res 1979; 7: 547-69.

14.

Chemey BW , McBridge OW , Chen D. et al. cDNA sequence, protein sequence and chromosomal location of the human gene for poly(ADPribose) polymerase. Proc Natl Acad Sci USA 1987; 84: 8370-4.

15.

Woolson RF Statistical Methods for the Analysis of Biomedical Data. New York, John Wiley, 1987.

16.

Haug BL , Lee JS , Sibley JT Altered metabolism of poly(ADP-Ribose) in family members of patients with SLE. J Rheumatol (in press).

Decreased mRNA Levels Coding for Poly(ADP-Ribose) Polymerase in Lymphocytes of Patients with SLE

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