Antiproliferative activity of nanofibers containing quaternized chitosan and/or doxorubicin against MCF-7 human breast carcinoma cell line by apoptosis

Abstract

The antiproliferative activity of electrospun mats of poly(L-lactide-co-D,L-lactide) (coPLA) containing quaternized chitosan (QCh) and/or doxorubicin hydrochloride (DOX) was evaluated against the Michigan Cancer Foundation-7(MCF-7) human breast carcinoma cell line. QCh- and DOX-containing nanofibrous mats possess good antiproliferative activity and decrease considerably the viability of the MCF-7 cells for the different periods of cell incubation as confirmed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Fluorescent microscopy analyses and scanning electron microscopy observations revealed that apoptosis was one of the major mechanisms of MCF-7 cell death induced by the QCh- and DOX-containing mats.

Keywords

electrospinning nanofibers quaternized chitosan polylactide antiproliferative activity apoptosis

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