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Abstract

Blue light sensitivity of melatonin suppression and subjective mood and alertness responses in humans is recognized as being melanopsin based. Observations that long-wavelength (red) light can potentiate responses to subsequent short-wavelength (blue) light have been attributed to the bistable nature of melanopsin whereby it forms stable associations with both 11-cis and all-trans isoforms of retinaldehyde and uses light to transition between these states. The current study examined the effect of concurrent administration of blue and red monochromatic light, as would occur in real-world white light, on acute melatonin suppression and subjective mood and alertness responses in humans. Young healthy men (18-35 years; n = 21) were studied in highly controlled laboratory sessions that included an individually timed 30-min light stimulus of blue (λ_max 479 nm) or red (λ_max 627 nm) monochromatic light at varying intensities (10¹³-10¹⁴ photons/cm²/sec) presented, either alone or in combination, in a within-subject randomized design. Plasma melatonin levels and subjective mood and alertness were assessed at regular intervals relative to the light stimulus. Subjective alertness levels were elevated after light onset irrespective of light wavelength or irradiance. For melatonin suppression, a significant irradiance response was observed with blue light. Co-administration of red light, at any of the irradiances tested, did not significantly alter the response to blue light alone. Under the current experimental conditions, the primary determinant of the melatonin suppression response was the irradiance of blue 479 nm light, and this was unaffected by simultaneous red light administration.

Keywords

light monochromatic humans melanopsin bistability melatonin suppression

Ocular light exposure in mammals can generate a range of acute nonvisual physiological and behavioral responses, which are maximally sensitive to short-wavelength blue light (λ_max ~ 480 nm). In humans, these include suppression of the nocturnal pineal hormone melatonin (Brainard et al., 2001; Thapan et al., 2001), circadian phase shifting (Lockley et al., 2003; Sletten et al., 2009), and enhancement of mood, alertness, and performance (Lockley et al., 2006; Revell et al., 2006). This blue light sensitivity in humans (λ_max 440-480 nm) is attributed to the photopigment melanopsin expressed in the inner retina in a subset of intrinsically photosensitive retinal ganglion cells (ipRGCs) (Berson et al., 2002; Gooley et al., 2001), which innervate brain areas regulating these nonvisual responses (Gooley et al., 2003; Hattar et al., 2006). In addition, the ipRGCs receive photic input from the rod and cone visual photoreceptors, which contribute to nonvisual responses in a context-dependent manner (Altimus et al., 2010; Lall et al., 2010).

Melanopsin is an atypical vertebrate opsin, being most homologous to invertebrate rhabdomeric opsins (Provencio et al., 2000) and having a sluggish, yet sustained, response to light (Berson et al., 2002; Dacey et al., 2005). Furthermore, through studies of human melanopsin (Melyan et al., 2005) and the Amphioxus homolog (Koyanagi et al., 2005), it has been suggested that melanopsin exhibits bistable properties and forms stable associations with both 11-cis (R, rhodopsin) and all-trans (M, metarhodopsin) isoforms of retinaldehyde, existing in equilibrium between these two states, with light driving their interconversion. The existence of such an intrinsic photoisomerase function to regenerate the chromophore (conversion of M to R state) removes the physical reliance on an external regeneration mechanism such as the retinal pigment epithelium. Although heterologous expression studies have indicated that the photosensitivity function (R to M state) is blue light sensitive (420-480 nm) (Panda et al., 2005), the photoisomerase function (M to R state) is purported to be long wavelength shifted (Melyan et al., 2005). This proposed sensitivity is supported by observations, in both murine suprachiasmatic nuclei single-cell recordings (Mure et al., 2007) and the human pupillary constriction response (Mure et al., 2009), where pretreatment with long-wavelength light (λ_max 620 nm) potentiated responses to subsequent blue light stimuli, presumably by increasing the available pool of the R state. Not all studies, however, have been able to demonstrate such potentiation (Mawad and Van Gelder, 2008), and the existence and physiological significance of melanopsin bistability remains an area of controversy. In addition to the potential existence of a long-wavelength sensitive melanopsin photoisomerase function, it is also suggested that the medium- and long-wavelength sensitive cone photoreceptors can contribute to human nonvisual light responses (Gooley et al., 2010). Combined, these observations suggest that the long-wavelength content of a polychromatic light pulse may be an important factor for driving nonvisual responses to light.

In the real world, it is likely that the proposed photosensitivity and photoisomerase functions of melanopsin will be stimulated simultaneously by short- and long-wavelength light, under both broadband polychromatic white lights and natural lighting (400-700 nm). This is different from the consecutive presentation of short and long wavelengths of light used in the previous in vivo studies (Mure et al., 2009; Mure et al., 2007). If melanopsin bistability is to be of practical use, then it is essential that the response to concurrent blue and red light is assessed. Therefore, in the current study, we aimed to determine whether the acute suppression of melatonin and elevation of mood and alertness by short-wavelength blue light (λ_max 479 nm) could be altered (enhanced or reduced) by simultaneous presentation of long-wavelength red (λ_max 627 nm) light.

Materials and Methods

All procedures were approved by the University of Surrey Ethics Committee and were conducted in accordance with the Declaration of Helsinki and conformed to international ethical standards. Participants gave written consent.

Participants

Healthy men (n = 21) aged 18 to 35 years (22.8 ± 3.5 years; mean ± SD) participated. As assessed by self-completed questionnaires, subjects were drug and prescription medication free, nonsmokers, and with no reported medical or psychological conditions. Participants had not crossed more than two time zones and/or worked night shifts during the month before the study. They had regular sleep-wake cycles with no reported sleep disorders (Pittsburgh Sleep Quality Index ≤ 5) and were neither extreme morning nor evening types (Horne-Östberg Morningness Eveningness Questionnaire score: 51.6 ± 6.6; mean ± SD). A full ophthalmic examination was performed on all participants to confirm they were free of any ocular disorders, including color vision deficiencies according to the Ishihara color-blindness plate test.

Prestudy measurements

The protocol used was similar to that previously described (Revell and Skene, 2007), with participants maintaining a regular, actigraphically (AWL, Cambridge Neurotechnology, UK) monitored sleep/wake schedule for at least 7 days before and throughout the in-laboratory sessions. For 72 h before each laboratory session and for its duration, participants refrained from caffeinated drinks, alcohol, excessive exercise, bright lights, and nonsteroidal anti-inflammatory drug intake. In addition, subjects performed a 48-h urine collection (sequential 4-h collection bins, 8 h overnight) for measurement, via radioimmunoassay, of 6-sulfatoxymelatonin, the major urinary metabolite of melatonin (Stockgrand Ltd., University of Surrey, Guildford, UK). The acrophase of 6-sulfatoxymelatonin production was determined by cosinor analysis, enabling light administration to be individually timed to occur on the rising phase of each participant’s endogenous melatonin rhythm.

In-laboratory protocol

Details of the protocol were previously described (Revell and Skene, 2007). A randomized, within-subject, crossover design was used. Participants were assigned to one of two study protocols that differed only in the light conditions administered (study A: 8 overnight sessions, n = 10; study B: 6 overnight sessions, n = 11). The sessions were run in blocks of 3 or 4 consecutive nights, which were separated by at least 3 days. The overnight in-laboratory sessions (1900-0700 h; October-April 2008-2010) contained a 2-h experimental period between 2300 and 0300 h. Night 1 was always a control condition (no light), and the subsequent light exposures were randomized across the remaining sessions. Throughout each study session posture, environmental lighting and meals were strictly controlled.

The participants’ pupils were fully dilated by a single drop of Minims Tropicamide (1.0%; Chauvin Pharmaceuticals, Romford, UK) placed in each eye 90 min before the light exposure. Blood samples were taken at regular 15-min intervals relative to the light exposure from 30 min before to up to 90 min after the start of the light pulse. The plasma fraction from each sample was stored at −20°C until melatonin analysis by radioimmunoassay. In addition, verbal ratings of subjective sleepiness (Karolinska Sleepiness Scale (Akerstedt and Gillberg, 1990) and mood and alertness (four 9-point scales: alertness, cheerfulness, depression, calmness) (Revell et al., 2006) were obtained at 15-min intervals from 15 min before until 45 min after the start of the light exposure.

Light Exposure

As previously described (Revell et al., 2010), the 30-min light pulse was administered using a specially constructed 45-cm diameter sphere (Apollo Lighting, Leeds, UK). The sphere was coated with white reflectance paint (WRC-680 Labsphere; Pro-Lite Technology, Bedfordshire, UK) and was illuminated via a fiber-optic cable connected to an ultra-high-pressure mercury lamp (Focus 100LS3, 100 W; Philips Lighting, Eindhoven, The Netherlands) to provide patternless illumination encompassing the entire visual field. Monochromatic light was produced using narrow-bandwidth interference filters (Coherent Ealing Europe Ltd., Watford, UK) with λ_max 480 and 630 nm (half-maximal bandwidth λ_0.5 10 nm). The actual in situ λ_max at eye level was shifted to 479 and 627 nm, respectively, as measured by a calibrated spectrometer (Ocean Optics BV, Dunedin, FL). Light irradiances were adjusted using neutral density filters (Kodak, Hemel Hempsted, UK) and were verified at the subject’s eye level using a calibrated radiometer (R203; Macam Photometrics Ltd., Livingston, Scotland) before and after each light exposure. Head movements and gaze were restricted, and subjects were monitored by trained researchers throughout the light stimulus.

Short-wavelength blue (λ_max 479 nm) and long-wavelength red (λ_max 627 nm) monochromatic lights were administered alone or in combination at a range of photon densities (Table 1). The photon densities were selected on the basis of previously determined irradiance response curves to monochromatic light for melatonin suppression (Thapan et al., 2001) and the reported spectral responses of the human pupil light reflex (Mure et al., 2009), to be within the melanopsin operating range and to ensure that either a potentiation or a reduction in the response could be observed. The highest irradiance of red monochromatic light tested was matched to that previously shown to be effective at enhancing subsequent pupil constriction responses to blue light (Mure et al., 2009). In study A, 3 irradiances of 479 nm light (10¹³-10¹⁴ photons/cm²/sec) were administered with and without a fixed irradiance (5 × 10¹³ photons/cm²/sec) of 627 nm light (n = 10). A subset of participants received 627 nm light alone at either 5 × 10¹³ photons/cm²/ sec (n = 5) or 1 × 10¹⁴ photons/cm²/sec (n = 4). In study B, a fixed irradiance of 479 nm light (2.5 × 10¹³ photons/cm²/sec) was administered alone and with varying irradiances of 627 nm light (10¹³-10¹⁴ photons/cm²/sec) (n = 11). A subset of participants received 627 nm light alone at 3 × 10¹⁴ photons/cm²/sec (n = 8).

Table 1.

Irradiances (photons/cm²/sec) of Monochromatic (λ_max 479 or 627 nm) and Bichromatic (λ_max 479 + 627 nm) Lights Administered in Study A (Fixed 627 nm Light Irradiance) and Study B (Fixed 479 nm Light Irradiance)

Study A: Fixed 627 nm Light Irradiance			Study B: Fixed 479 nm Light Irradiance
Photon Density (photons/cm²/sec)			Photon Density (photons/cm²/sec)
479 nm	627 nm	479 + 627 nm	479 nm	627 nm	479 + 627 nm
1 × 10¹³	5 × 10¹³	6 × 10¹³	2.5 × 10¹³	5 × 10¹³	7.5 × 10¹³
5 × 10¹³	5 × 10¹³	1 × 10¹⁴	2.5 × 10¹³	1 × 10¹⁴	1.3 × 10¹⁴
1 × 10¹⁴	5 × 10¹³	1.5 × 10¹⁴	2.5 × 10¹³	3 × 10¹⁴	3.3 × 10¹⁴

Study A: 479 nm light administered at 3 irradiances with and without concurrent 627 nm light of fixed irradiance. Study B: 479 nm light administered at a fixed irradiance alone and in combination with 3 increasing irradiances of 627 nm light.

Analysis and statistics

Plasma was separated within 10 min of collection by refrigerated centrifugation (3000 rpm for 10 min) and stored at −20 14;°C until analysis. Plasma melatonin was assayed by radioimmunoassay (affinity-stripped plasma and antimelatonin antiserum G/S/704-8483, Stockgrand Ltd., University of Surrey, Guildford, UK; tritiated melatonin TRK-798, Amersham International Ltd., Buckinghamshire, UK) (Fraser et al., 1983). The limit of detection was 6 pg/mL, and the interassay coefficients of variation were 16% at 25 pg/mL, 8% at 89 pg/mL, and 8% at 112 pg/mL (n = 26 for each concentration). Melatonin profiles suitable for analysis were obtained from 20 of the 21 participants (study A, n = 10; study B, n = 10). Percentage melatonin suppression was calculated as the change in melatonin levels from 0 to 30 min as a percentage of the 0-min levels (Revell et al., 2010). Mood and alertness scores at each time point (−15, 0, 15, 30, and 45 min) were expressed relative to an individual’s score at 0 min for each light condition. For clarity, the alertness, cheerfulness, and calmness ratings were reverse-scored such that a higher rating indicated increased mood and alertness. All statistical analysis was carried out using SPSS 16.0 (SPSS, Inc., Chicago, IL). The significance level was set at p ≤ 0.05, and significant results were compared post hoc by Bonferroni test. For melatonin raw data, paired Student t test was used to compare pre-exposure (0 min) and postexposure (30 min) plasma melatonin concentrations separately for each light condition. In addition, repeated-measures analysis of variance (ANOVA) was used to compare the 0-min plasma melatonin concentration between light conditions. For the remaining analysis, repeated-measures ANOVA was used with the following factors: melatonin suppression: (1) 479 nm vs. 627 nm, wavelength and time; (2) study A, red light presence and blue light irradiance; and (3) study B, red light irradiance. For mood and alertness: study A, red light presence, blue light irradiance and time; study B, red light irradiance and time.

Results

The ability of blue (λ_max 479 nm) and red (λ_max 627 nm) monochromatic light, of varying photon densities presented alone and in combination, to influence plasma melatonin levels and subjective mood and alertness was quantified in healthy male participants.

Minimal suppression of melatonin range (−2.9% to 16.7%; n = 17) was observed during the administration of 627 nm light alone at all photon densities tested (5 × 10¹³ to 3 × 10¹⁴ photons/cm²/sec). At all photon densities, the observed melatonin suppression with 627 nm light did not significantly differ from that of the “no-light” baseline condition as demonstrated by within-subject comparisons (data not shown). Representative plasma melatonin profiles (raw data) from 3 participants receiving the no-light control as well as 627 nm, 479 nm, and 479 + 627 nm light at varying irradiances are shown in Figure 1.

Figure 1.

Plasma melatonin profiles (pg/mL) during and after a 30-min light stimulus (shaded area) in 3 participants under no-light control conditions, red (627 nm) monochromatic, blue (479 nm) monochromatic, and red and blue bichromatic (479 + 627 nm) light. (A) Participant 1, 627: 5 × 10¹³ photons/cm²/sec nm, 479 nm: 5 × 10¹³ photons/cm²/sec, 479 + 627 nm: 1 × 10¹⁴ photons/cm²/sec. (B) Participant 2, 627 nm: 1 × 10¹⁴ photons/cm²/sec, 479 nm: 1 × 10¹⁴ photons/cm²/sec, 479 + 627 nm: 1.5 × 10¹⁴ photons/cm²/sec. (C) Participant 3, 627 nm: 3 × 10¹⁴ photons/cm²/sec, 479 nm: 2.5 × 10¹³ photon/cm²/sec, 479 + 627 nm: 3.3 × 10¹⁴ photon/cm²/sec.

Blue (479 nm) light produced significantly greater melatonin suppression than photon-matched red (627 nm) light as evidenced by within-subject comparisons at 5 × 10¹³ and 1 × 10¹⁴ photons/cm²/sec (Figure 2A, F_{(1, 4)} = 14.9, p = 0.018, n = 5; Figure 2B, F_{(1, 3)} = 25.7, p = 0.015, n = 4), respectively. Furthermore, the lowest irradiance 479 nm light (2.5 × 10¹³ photons/cm²/sec) was significantly more effective at suppressing melatonin than a 12-fold brighter 627 nm light pulse (3 × 10¹⁴ photons/cm²/sec) (Figure 2C, F_{(1, 7)} = 11.4, p = 0.012, n = 8).

Figure 2.

Percentage melatonin suppression (mean ± SEM) during a 30-min nocturnal red (627 nm) or blue (479 nm) monochromatic light pulse (shaded area) in the same individuals. (A) 5 × 10¹³ photons/cm²/sec (n = 5). (B) 1 × 10¹⁴ photons/cm²/sec light pulses (n = 4). (C) 627 nm (3 × 10¹⁴ photons/cm²/sec) and 479 nm (2.5 × 10¹³ photon/cm²/sec) light pulses (n = 8).

Melatonin suppression was calculated by comparing the plasma melatonin levels before (0 min) and at the end (30 min) of the light stimulus. For each study, the levels at 0 min did not differ significantly between light conditions (including the no-light control) (study A: 56.7-64.0 pg/mL, mean range; 59.4 ± 5.6 pg/mL, mean ± SEM; F_{(6, 54)} = 0.8, p = 0.489, n = 10; study B: 65.3-74.8 pg/mL; 70.1 ± 3.5 pg/mL; F_{(4, 36)} = 2.4, p = 0.066, n = 10). At 30 min, under the baseline no-light condition, plasma melatonin levels were significantly elevated (p < 0.05), whereas under 479 nm light at 5 × 10¹³ and 1 × 10¹⁴ photons/cm²/sec, melatonin levels were significantly suppressed (p < 0.01) (Figure 3).

Figure 3.

Plasma melatonin levels (mean ± SEM; n = 10) before (0 min) and after (30 min) monochromatic blue (479 nm) light stimuli of increasing irradiance; the no-light control condition is indicated by 0. *p < 0.05; **p < 0.01, compared to the corresponding 0-min condition.

In study A, irradiance dependency of melatonin suppression was observed for both 479 nm light alone and 479 + 627 nm light pulses (Figure 4A) (F_{(2, 18)} = 17.3, p < 0.001). However, no significant effects of red light presence (479 nm alone vs 479 + 627 nm) or red light presence × blue light irradiance interaction were observed. Similarly, in study B, the melatonin suppression observed with 479 nm light alone (2.5 × 10¹³ photons/cm²/sec) did not differ significantly from that obtained with bichromatic pulses containing 479 nm light and increasing irradiances of 627 nm light (Figure 4B) (F_{(3, 27)} = 2.1, p = 0.122).

Figure 4.

Percentage melatonin suppression (mean ± SEM) at the end of a 30-min nocturnal monochromatic blue light stimulus (λ_max 479 nm) alone and in combination with long-wavelength red light (λ_max 627 nm). (A) Increasing irradiances of 479 nm light alone and in combination with 5 × 10¹³ photons/cm²/sec 627 nm light (n = 10). (B) 479 nm (2.5 × 10¹³ photons/cm²/sec) light alone and in combination with increasing irradiances of red 627 nm light (n = 10).

At lower photon densities (5 × 10¹³ photons/cm²/sec), 479 nm light significantly (p = 0.049) enhanced subjective alertness ratings compared to 627 nm light as demonstrated by within-subject comparisons (data not shown). In study A, there was a significant effect of time on subjective alertness (F_{(4, 36)} = 3.3, p = 0.020) (Figure 5A) and sleepiness (F_{(4, 36)} = 3.3, p = 0.022) but no significant effect of light condition or light condition × time interaction. A significant light condition × time interaction for depression was revealed (F_{(4, 36)} = 2.9, p = 0.035). In study B, a significant effect of time was observed for alertness (F_{(4, 40)} = 7.9, p = 0.003) (Figure 5B), calmness (F_{(4, 40)} = 8.8, p < 0.001), depression (F_{(4, 40)} = 5.1, p = 0.002), and sleepiness (F_{(4, 40)} = 6.1, p = 0.006) levels but no significant effect of light condition or light condition × time interaction.

Figure 5.

Subjective alertness levels (mean ± SEM) before, during, and after a nocturnal 30-min monochromatic or dichromatic light stimulus normalized to 0 min. (A) 479 nm light alone at 3 irradiances, 1 × 10¹³ photons/cm²/sec (●), 5 × 10¹³ photons/cm²/sec (□), and 1 × 10¹⁴ photons/cm²/sec (▲), and in combination with fixed 5 × 10¹³ photons/cm²/sec λ_max 627 nm light resulting in 6 × 10¹³ photons/cm²/sec (▽), 1 × 10¹⁴ photons/cm²/sec (X), and 1.5 × 10¹⁴ photons/cm²/sec (○) (n = 10). (B) Fixed 2.5 × 10 photons/cm²/sec irradiance of blue λ_max 479 nm light alone (●) and in combination with increasing irradiances of red λ_max 627 nm light resulting in 7.5 × 10¹³ photons/cm²/sec (□), 1.3 × 10¹⁴ photons/cm²/sec (▲), and 3.3 × 10¹⁴ photons/cm²/sec (▽) (n = 11). A significant effect of time but not of light condition was observed for both studies.

Discussion

The current study aimed to test the hypothesis that acute melatonin and subjective measures of alertness and mood to short-wavelength blue light (λ_max 479 nm) could be significantly altered by concurrent presentation of long-wavelength red light (λ_max 627 nm).

In accordance with previous studies of light-induced melatonin suppression (Brainard et al., 2001; Thapan et al., 2001), we observed a clear irradiance dependency with blue monochromatic light. In addition, minimal melatonin suppression (non–control adjusted) was observed with red monochromatic light. This is in agreement with a previous study (Hanifin et al., 2006) where a 90-min red monochromatic light stimulus (1.9 × 10¹⁸ photons/cm²), over 6000 times brighter than our highest photon density red light, only induced 18% melatonin suppression (control adjusted) that did not significantly differ from the no-light control response (Hanifin et al., 2006).

The current study demonstrates for the first time that simultaneous presentation of red 627 nm light neither enhanced nor reduced the degree of melatonin suppression observed with blue 479 nm light alone. This was true when a fixed-irradiance red light pulse (5 × 10¹³ photons/cm²/sec) was added to increasing irradiances of blue light (study A) and when a fixed-irradiance blue light pulse (2.5 × 10¹³ photons/cm²/sec) was administered with increasing irradiances of red light (study B). At lower irradiances (5 × 10¹³ photons/cm²/s), 479 nm light was more effective at enhancing subjective alertness levels than photon-matched 627 nm light. The lack of this discrepancy at higher irradiances suggests that at these levels, either the irradiance of the light is more determinative than wavelength for alertness or the subjective scales lack sufficient resolution at higher photon densities. Similar to the melatonin suppression data, the addition of a fixed-irradiance red light did not enhance the alerting response to blue light alone. The observed photic enhancement of subjective mood and alertness at night, irrespective of light irradiance and composition, in dark-adapted individuals is in accordance with our previous data (Revell et al., 2010).

Previous studies in mice and humans (Mure et al., 2009; Mure et al., 2007) have attributed the ability of a long-wavelength red light pulse to enhance responses to subsequent blue light exposure to the bistable nature of melanopsin. It has been proposed that, through red light stimulation of the M state, the proportion of R state available for subsequent stimulation by blue light will be increased, resulting in an augmented response. By contrast, concurrent presentation of red and blue light, as used in the present study, has the theoretical potential to both increase and reduce responses observed with blue light alone. The action of red light on the pool of M state could either enhance the response by increasing the available proportion of “blue light–responsive” R state or reduce the response by simply attenuating the sustained firing of the M state under prolonged exposure to blue light. In addition, the presence of longer wavelength red light could have stimulated the M- and L-cone photoreceptors whose input could have also modulated the response to blue light alone. Indeed, a limitation of the current experimental design (and all previous human studies) is that the relative contribution of each photoreceptor class cannot be isolated; rather, the responses measured result from the aggregate input of all 3 classes of photoreceptors. Increasing evidence suggests that, under specific conditions, rods and cones might be important in regulating nonvisual responses to light (Lucas et al., 2003; Altimus et al., 2010; Lall et al., 2010). Studying patients with retinal disorders who lack specific classes of a classical photoreceptor may provide information into the response dynamics of melanopsin.

The lack of significant impact of the concurrent presentation of red and blue light in the current study compared to the observed enhancing effect of consecutive red and blue light for the pupil light reflex (PLR) (Mure et al., 2009) may be attributable to differences in the protocol such as time of day, light stimulus duration, periods of darkness, and dependent variables. First, the PLR was measured in the morning compared to our nocturnal assessments of melatonin suppression; circadian variation in photopigment contribution to nonvisual responses has been observed previously (Tsai et al., 2009) and could be pertinent here. Second, it is possible that, under concurrent red and blue light administration over a 30-min period, the red light simultaneously enhanced (regenerating R state) and repressed (attenuating M state firing) the response to blue light, resulting in no overall change in response. Third, as the relative sensitivities of the M and R states are unknown, it is possible that the irradiances of red light used in the current studies were insufficient to activate the M state under constant blue light illumination. Finally, it is possible that regeneration of the R state under constant illumination (current study) may be slower than under complete darkness (PLR study) such that we observed neither potentiation nor suppression of response. The dynamics of the photoisomerase (M to R state) function under both light and dark conditions remain to be determined. A protocol regime incorporating an extended period of darkness between the red and blue light stimuli may be necessary to resolve the photoisomerase function.

The bistability of melanopsin is an emerging area, and it is possible that, in the current study, the optimal long wavelength and/or irradiance light, to demonstrate photoisomerase activity, was not used. Although the in vivo studies of Mure and colleagues (2007, 2009) implicated wavelengths >600 nm (used in the current study), exposure to blue light in Amphioxus results in an only slightly red-shifted photoproduct (520 nm) that was then capable of photoregenerating the bleached isoform (Koyanagi et al., 2005). Further complicating the interpretation of the present and previous results is the proposed existence of a light-independent (dark) regeneration mechanism (Koyanagi et al., 2005) as well as the possibility of more than one meta-state (Ritter et al., 2004). Melanopsin in dark-adapted mouse retinas has been shown to be exclusively bound to 11-cis retinal (Walker et al., 2008), and this apparent photo-independent regeneration may have contributed to the results observed by Mure and colleagues (Mure et al., 2009; Mure et al., 2007). Thus, regeneration of the R state may be a highly complex process, and identification of photoisomerase activity may be protocol dependent. Finally, the existence of several subtypes of melanopsin ipRGCs that differentially innervate different brain areas involved in nonvisual responses (Baver et al., 2008; Ecker et al., 2010) and are themselves differentially reliant on rod/cone signaling (Schmidt and Kofuji, 2010) could also contribute to the response diversity observed between the PLR (Mure et al., 2009) and the melatonin suppression responses (current study).

Establishing whether concurrent administration of red light can significantly alter nonvisual responses to blue light is important as this experimental paradigm is likely to be more practical and applicable than the consecutive light pulses used in previous studies (Mure et al., 2009). Under the current experimental conditions, there was no evidence of simultaneous red light significantly altering nonvisual responses to blue light alone. Future optimization of lighting for health and well-being will require the relative contribution of each photoreceptor class to be deciphered as well as the complexities of the melanopsin states to be defined. Recent work in transgenic mice lacking rods and cones has demonstrated that the melanopsin photosensitivity function alone can predict circadian and pupil responses to a diverse range of poly-chromatic lights (Enezi et al., 2011). These data are in agreement with the current study and, although not precluding the existence of melanopsin bistability, suggest that the red long-wavelength content of a light stimulus may not be a significant factor contributing to nonvisual responses to polychromatic light.

Footnotes

Acknowledgements

This work was supported by the 6th Framework project EUCLOCK (018741).

The authors thank Philips Lighting, Eindhoven, The Netherlands, for providing the light sources, and Stockgrand Ltd. for providing radioimmunoassay reagents. They also thank Daniel Barrett, Dr. Shelagh Hampton, Dr. Benita Middleton, Ivonne Vogt, and the Surrey CRC clinical and medical teams for their assistance.

The author(s) have declared the following potential conflicts of interest with respect to the research, authorship, and/or publication of this article: V.L.R. acts as a scientific advisor to Lumie Ltd, Cambridge, UK. D.J.S. is a director of Stockgrand Ltd, Guildford, UK.

References

Akerstedt

Gillberg

(1990) Subjective and objective sleepiness in the active individual. Int J Neurosci 52:29-37.

Altimus

Guler

Alam

Arman

Prusky

Sampath

Hattar

(2010) Rod photoreceptors drive circadian photoentrainment across a wide range of light intensities. Nat Neurosci 13:1107-1112.

Baver

Pickard

Sollars

(2008) Two types of melanopsin retinal ganglion cell differentially innervate the hypothalamic suprachiasmatic nucleus and the olivary pretectal nucleus. Eur J Neurosci 27:1763-1770.

Berson

Dunn

Takao

(2002) Phototransduction by retinal ganglion cells that set the circadian clock. Science 295:1070-1073.

Brainard

Hanifin

Greeson

Byrne

Glickman

Gerner

Rollag

(2001) Action spectrum for melatonin regulation in humans: evidence for a novel circadian photoreceptor. J Neurosci 21:6405-6412.

Dacey

Liao

Peterson

Robinson

Smith

Pokorny

Yau

Gamlin

(2005) Melanopsin-expressing ganglion cells in primate retina signal colour and irradiance and project to the LGN. Nature 433:749-754.

Ecker

Dumitrescu

Wong

Alam

Chen

LeGates

Renna

Prusky

Berson

Hattar

(2010) Melanopsin-expressing retinal ganglion-cell photoreceptors: cellular diversity and role in pattern vision. Neuron 67:49-60.

Enezi

Revell

Brown

Wynne

Schlangen

Lucas

(2011) A “melanopic” spectral efficiency function predicts the sensitivity of melanopsin photoreceptors to polychromatic lights. J Biol Rhythms 26:314-323.

Fraser

Cowen

Franklin

Franey

Arendt

(1983) Direct radioimmunoassay for melatonin in plasma. Clin Chem 29:396-397.

10.

Gooley

Chou

Scammell

Saper

(2001) Melanopsin in cells of origin of the retino-hypothalamic tract. Nat Neurosci 4:1165.

11.

Gooley

Fischer

Saper

(2003) A broad role for melanopsin in nonvisual photoreception. J Neurosci 23:7093-7106.

12.

Gooley

Rajaratnam

Brainard

Kronauer

Czeisler

Lockley

(2010) Spectral responses of the human circadian system depend on the irradiance and duration of exposure to light. Sci Transl Med 2:31ra33.

13.

Hanifin

Stewart

Smith

Tanner

Rollag

Brainard

(2006) High-intensity red light suppresses melatonin. Chronobiol Int 23:251-268.

14.

Hattar

Kumar

Park

Tong

Tung

Yau

Berson

(2006) Central projections of melanopsin-expressing retinal ganglion cells in the mouse. J Comp Neurol 497:326-349.

15.

Koyanagi

Kubokawa

Tsukamoto

Shichida

Terakita

(2005) Cephalochordate melanopsin: evolutionary linkage between invertebrate visual cells and vertebrate photosensitive retinal ganglion cells. Curr Biol 15:1065-1069.

16.

Lall

Revell

Momiji

Al Enezi

Altimus

Guler

Aguilar

Cameron

Allender

Hankins

. (2010) Distinct contributions of rod, cone, and melanopsin photoreceptors to encoding irradiance. Neuron 66:417-428.

17.

Lockley

Brainard

Czeisler

(2003) High sensitivity of the human circadian melatonin rhythm to resetting by short wavelength light. J Clin Endocrinol Metab 88:4502-4505.

18.

Lockley

Evans

Scheer

Brainard

Czeisler

Aeschbach

(2006) Short-wavelength sensitivity for the direct effects of light on alertness, vigilance, and the waking electroencephalogram in humans. Sleep 29:161-168.

19.

Lucas

Hattar

Takao

Berson

Foster

Yau

(2003) Diminished pupillary light reflex at high irradiances in melanopsin-knockout mice. Science 299:245-247.

20.

Mawad

Van Gelder

(2008) Absence of long-wavelength photic potentiation of murine intrinsically photosensitive retinal ganglion cell firing in vitro. J Biol Rhythms 23:387-391.

21.

Melyan

Tarttelin

Bellingham

Lucas

Hankins

(2005) Addition of human melanopsin renders mammalian cells photoresponsive. Nature 433:741-745.

22.

Mure

Cornut

Rieux

Drouyer

Denis

Gronfier

Cooper

(2009) Melanopsin bistability: a fly’s eye technology in the human retina. PLoS One 4:e5991.

23.

Mure

Rieux

Hattar

Cooper

(2007) Melanopsin-dependent nonvisual responses: evidence for photopigment bistability in vivo. J Biol Rhythms 22:411-424.

24.

Panda

Nayak

Campo

Walker

Hogenesch

Jegla

(2005) Illumination of the melanopsin signaling pathway. Science 307:600-604.

25.

Provencio

Rodriguez

Jiang

Hayes

Moreira

Rollag

(2000) A novel human opsin in the inner retina. J Neurosci 20:600-605.

26.

Revell

Arendt

Fogg

Skene

(2006) Alerting effects of light are sensitive to very short wavelengths. Neurosci Lett 399:96-100.

27.

Revell

Barrett

Schlangen

Skene

(2010) Predicting human nocturnal nonvisual responses to monochromatic and polychromatic light with a melanopsin photosensitivity function. Chronobiol Int 27:1762-1777.

28.

Revell

Skene

(2007) Light-induced melatonin suppression in humans with polychromatic and monochromatic light. Chronobiol Int 24:1125-1137.

29.

Ritter

Zimmermann

Heck

Hofmann

Bartl

(2004) Transition of rhodopsin into the active metarhodopsin II state opens a new light-induced pathway linked to Schiff base isomerization. J Biol Chem 279:48102-48111.

30.

Schmidt

Kofuji

(2010) Differential cone pathway influence on intrinsically photosensitive retinal ganglion cell subtypes. J Neurosci 30:16262-16271.

31.

Sletten

Revell

Middleton

Lederle

Skene

(2009) Age-related changes in acute and phase-advancing responses to monochromatic light. J Biol Rhythms 24:73-84.

32.

Thapan

Arendt

Skene

(2001) An action spectrum for melatonin suppression: evidence for a novel non-rod, non-cone photoreceptor system in humans. J Physiol 535:261-267.

33.

Tsai

Hannibal

Hagiwara

Colas

Ruppert

Ruby

Heller

Franken

Bourgin

(2009) Melanopsin as a sleep modulator: circadian gating of the direct effects of light on sleep and altered sleep homeostasis in Opn4(−/−) mice. PLoS Biol 7:e1000125.

34.

Walker

Brown

Cronin

Robinson

(2008) Photochemistry of retinal chromophore in mouse melanopsin. Proc Natl Acad Sci U S A 105:8861-8865.

Human Nonvisual Responses to Simultaneous Presentation of Blue and Red Monochromatic Light

Abstract

Keywords

Materials and Methods

Participants

Prestudy measurements

In-laboratory protocol

Light Exposure

Analysis and statistics

Results

Discussion

Footnotes

Acknowledgements

References