Rhodococcus equi was isolated from the lungs of six foals with bronchopneumonia. All isolates expressed 15-17-kd antigens by immunoblot analysis and contained a virulence-associated plasmid of 85 or 90 kb. Immunohistochemically, R. equi from all pulmonary lesions showed the expression of 15-17-kd antigens mainly in the phagocytic cells. The specific monoclonal antibody to 15-17-kd antigens of R. equi (MAb 10G5) may be an aid in the diagnosis of R. equi-induced pneumonia.
BartonMDHughesKL:
Corynebacterium equi: a review.
Vet Bull50:
65–80,
1980
2.
DungworthDL:
Rhodococcus (Corynebacterium) equi infection.
In:
Pathology of Domestic Animals,
ed. JubbKVFKennedyPCPalmerN,
4th ed.,
pp. 652–655.
Academic Press,
San Diego, CA,
1993
3.
IshinoSKumagaiKKuniyoshiSNakazawaMMatsudaIOkaM:
Immunohistochemical observations on pneumonic lesions caused by Rhodococcus equi in foals.
J Vet Med Sci54:
509–515,
1992
4.
PlattH:
Septicaemia in the foal. A review of 61 cases.
Br Vet J129:
221–229,
1973
5.
PrescottJF:
Rhodococcus equi: an animal and human pathogen.
Clin Microbiol Rev4:
20–34,
1991
6.
TakaiSAnzaiTSasakiYTsubakiSKamadaM:
Virulence of Rhodococcus equi isolated from lesions of infected foals.
Bull Equine Res Inst30:
9–14,
1993
7.
TakaiSIieMKobayashiCMorishitaTNishioTIshidaTFujimuraTSasakiYTsubakiS:
Monoclonal antibody specific to virulence-associated 15- to 17-kilodalton antigens of Rhodococcus equi.
J Clin Microbiol31:
2780–2782,
1993
8.
TakaiSKoikeKOhbushiSIzumiCTsubakiS:
Identification of 15- to 17-kilodalton antigens associated with virulent Rhodococcus equi.
J Clin Microbiol29:
439–443,
1991
9.
TakaiSSekizakiTOzawaTSugawaraTWatanabeYTsubakiS:
Association between a large plasmid and 15- to 17-kilodalton antigens in virulent Rhodococcus equi.
Infect Immun59:
4056–4060,
1991