Involvement of CD8 + T Cells in Delayed-type Hypersensitivity Responses against Bovine Leukemia Virus (BLV) Induced in Sheep Vaccinated with Recombinant Vaccinia Virus Expressing BLV Envelope Glycoprotein
Free accessResearch articleFirst published online March, 1993
Involvement of CD8 + T Cells in Delayed-type Hypersensitivity Responses against Bovine Leukemia Virus (BLV) Induced in Sheep Vaccinated with Recombinant Vaccinia Virus Expressing BLV Envelope Glycoprotein
Delayed-type hypersensitivity responses against bovine leukemia virus (BLV) envelope glycoprotein (gp60) were induced in the skin of sheep vaccinated with recombinant vaccinia virus (RVV) expressing BLV glycoprotein. The lesions were characterized by marked infiltration of lymphocytes, slight migration of neutrophils, eosinophils, and macrophages in the dermis to hypodermis, and partial intercellular edema in the reticular layer. Immunohistochemical analysis with monoclonal antibodies demonstrated that the lymphocytic infiltrates consisted mainly of CD8+ T cells (53.7–55.8% at 48 hours post-challenge of BLV), CD4+ T cells (24.7–26.7%), and B cells (11.5–16.9%). The role of CD4+ and CD8+ T cells in suppressing BLV growth in RVV-vaccinated animals is discussed.
AidaYMiyasakaMOkadaKOnumaMKogureSSuzukiMMinoprioPLevyDIkawaY:
Further phenotypic characterization of target cells for bovine leukemia virus experimental infection in sheep.
Am J Vet Res50:
1946–1951,
1989
2.
DavisWCHamiltonMJParkYLarsenRAWyattCROkadaK:
Ruminant leukocyte differentiation molecules.
In:
Monographs in Animal Immunology,
vol. I,
pp. 47–70.
BAR-LAB,
Blacksburg, VA,
1990
3.
DavisWCMarusicSLewinHASplitterGAPerrymanLEMcGuireTCGorhamJR:
The development and analysis of species specific and cross reactive monoclonal antibodies to leukocyte differentiation antigens and antigens of major histocompatibility complex for use in the study of the immune system in cattle and other species.
Vet Immunol Immunopathol15:
337–376,
1987
4.
DohertyPCAllanJELynchFCeredigR:
Dissection of an inflammatory process induced by CD8+ T cells.
Immunol Today11:
55–59,
1990
5.
EzakiTParisotRDudlerLBeyaMFMiyasakaMTrnkaZ:
Monoclonal antibodies to surface markers which define functional subsets of sheep T lymphocytes.
In:
Immunology of the Sheep,
ed. MorrisBMiyasakaM,
pp. 88–110.
F. Hoffmann-La Roche,
Basel, Switzerland,
1985
6.
FerrerJF:
Antigenic comparison of bovine type C virus with murine and feline leukemia virus.
Cancer Res32:
1871–1877,
1972
7.
FerrerJFAbtDABhattDMMarshakRR:
Studies on the relationship between infection with bovine C-type virus, leukemia, and persistent lymphocytosis in cattle.
Cancer Res34:
893–900,
1974
8.
HsuSMRaineLFangerH:
A comparative study of the peroxidase-antiperoxidase method and an avidinbiotin complex method for studying polypeptide hormones with radioimmunoassay antibodies.
Am J Clin Pathol75:
734–738,
1981
9.
International Committee on Bovine Leukosis:
Criteria for the determination of the normal and leukotic state in cattle.
J Natl Cancer Inst41:
243–263,
1961
10.
KleinJ:
The cell.
In:
Natural History of the Major Histocompatibility Complex,
pp. 291–422.
John Wiley & Sons,
New York, NY1986
11.
KoyamaHHohdatsuTNagaiTTsubakiS:
Determination of lymphocyte count necessary for isolating bovine leukemia virus (BLV) from BLV-infected cattle and correlation between lymphocyte count and antibody titer.
J Vet Med Ser B35:
648–653,
1988
12.
LanierLLPhillipsJHHackettJTuttMKumarV:
Opinion. Natural killer cells: definition of a cell type rather than a function.
J Immunol137:
2735–2739,
1986
13.
LevyDDeshayesLGuillemainBParodiAL:
Bovine leukemia virus specific antibodies among French cattle. I. Comparison of complement fixation and hematological test.
Int J Cancer19:
822–827,
1977
14.
MackayCRHeinWRBrownMHMatzingerP:
Unusual expression of CD2 in sheep: implications for T cell interactions.
Eur J Immunol18:
1681–1688,
1988
15.
MammerickxMPortetelleDKettmannRGhysdaelJBurnyADekegelD:
Diagnostic tests of bovine leukemia. Comparison between an hematological test and the serological diagnosis.
Eur J Cancer12:
433–439,
1976
16.
MillerJMMillerLDOlsonCGilletteKG:
Virus-like particles in phytohemagglutinin-stimulated lymphocyte cultures with reference to bovine lymphosarcoma.
J Natl Cancer Inst43:
1297–1305,
1969
17.
MiyasakaMMcClureSHeinWRTrnkaZ:
Differentiation antigens in lymphohemopoietic system of the sheep.
In:
Differentiation Antigens in Lymphohemopoietic Tissues,
ed. MiyasakaMTrnkaZ,
pp. 499–523.
Marcel Dekker,
New York, NY,
1988
18.
OhishiKSuzukiHMaruyamaTYamamotoTFunahashiSMikiKIkawaYSugimotoM:
Induction of neutralizing antibodies against bovine leukosis virus in rabbits by vaccination with recombinant vaccinia virus expressing bovine leukosis virus envelope glycoprotein.
Am J Vet Res51:
1170–1173,
1990
19.
OhishiKSuzukiHYamamotoTMaruyamaTMikiKIkawaYNumakunaiSOkadaKOhshimaKSugimotoM:
Protective immunity against bovine leukaemia virus (BLV) in carrier sheep induced by inoculation with the vaccinia virus-BLV env recombinant: association with cell-mediated immunity.
J Gen Virol72:
1887–1892,
1991
20.
YamamotoSOnumaMKodamaHKoyamaHMikamiTIzawaH:
Existence of cytotoxic activity against BLV-transformed cells in lymphocytes from normal cattle and sheep.
Vet Immunol Immunopathol8:
63–78,
1985
21.
YamamotoSOnumaMKodamaHMikamiTIzawaH:
Suppression of natural cytotoxic activity of lymphocytes from cattle and sheep during the progress of bovine leukosis.
Vet Microbiol9:
105–111,
1984
