Abstract
Interleukin-1β transfer across the blood–brain barrier in the ovine fetus
Grazyna B Sadowska, Xiaodi Chen, Jiyong Zhang, Yow-Pin Lim, Erin E Cummings, Oleksandr Makeyev, Walter G Besio, John Gaitanis, James F Padbury, William A Banks and Barbara S Stonestreet
Journal of Cerebral Blood Flow & Metabolism 2015; 35:
On page 1389 of this article, the text read as follows when first published:
Radiolabeling of the IL-1β Ovine Protein. On the day of the study, the pure IL-1β protein was radioactively labeled with iodine-125 (125I-IL-1β) using the chloramine-T method as previously described.26 One µCi of 125I-, 5 µg of IL-1β protein, and 10 µL of 1 mg/ml chloramine-T (Sigma-Aldrich, St. Louis, MO, USA) were combined and vigorously mixed. After 60 seconds, the reaction was stopped with 10 µL of 10 mg/ml sodium metabisulfite (Sigma, St. Louis, MO, USA).
The text should have read as follows:
Radiolabeling of the IL-1β Ovine Protein. On the day of the study, the pure IL-1β protein was radioactively labeled with iodine-125 (125I-IL-1β) using the chloramine-T method as previously described.26 Two mCi of 125I-, 5 µg of IL-1β protein, and 10 µL of 1 mg/ml chloramine-T (Sigma-Aldrich, St. Louis, MO, USA) were combined and vigorously mixed. After 60 seconds, the reaction was stopped with 10 µL of 10 mg/ml sodium metabisulfite (Sigma, St. Louis, MO, USA).