Estimation of LC50 Values by Assay of Lactate Dehydrogenase and DNA Redistribution in Human Lymphocyte Cultures

Abstract

The acute toxicities of selected drugs were assayed biochemically in vitro by estimation of LC50 dose in human lymphocyte cultures. Two methods were compared for estimation of LC50 values, the redistribution of DNA and lactate dehydrogenase after treatment with various concentrations of the compound. Ten of the chemicals used had previously been tested in HeLa cells using morphological changes as the endpoint. The LC50 values found in this study were a little lower than the IC50 values for HeLa cells. The enzyme assay gave lower LC50 values than the DNA assay, but the differences were not significant. LC50 values were also found for two drugs tested in several cell lines with redistribution of DNA and proteins. Again, no significant differences were apparent.

Keywords

LC 50 human lymphocytes redistribution of DNA lactate dehydrogenase

Get full access to this article

View all access options for this article.

References

Ekwall

, and Johanson

(1980). Preliminary studies on the validity of in vitro measurement of drug toxicity using HeLa cells, I. Comparative in vitro cytotoxicity of 27 drugs. Toxicology Letters 5, 299–307.

Ekwall

(1980). Preliminary studies on the validity of in vitro measurement of drug toxicity using HeLa cells, II. Drug toxicity in the MIT-24 system compared with mouse and human lethal dosage of 52 drugs. Toxicology Letters 5, 309–317.

Balls

, and Horner

S.A.

(1985). The FRAME interlaboratory programme on in vitro cytotoxicology. Food and Chemical Toxicology 23, 209–213.

Ekwall

, and Acosta

(1982). In vitro comparative toxicity of selected drugs and chemicals in HeLa cells, Chang liver cells and rat hepatocytes. Drug and Chemical Toxicology 5, 219–231.

Bergmeyer

H.U.

(1970). Methoden der Enzymatischen Analyse, 2nd edn, (ed. Trautschold

, & Löffler

) pp. 182–183, Verlag Chemie Weinheim.

Kissane

J.M.

, and Robins

(1958). The fluorometric measurement of deoxyribonucleic acid in animal tissues with special reference to the central nervous system. Journal of Biological Chemistry 233, 184–188.

Fotina

, Merson

E.J.

, and Allan

F.H.

(1971). Micromethod for rapid separation of lymphocytes from peripheral blood. Annals of Clinical and Laboratory Science 1, 131–133.