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Abstract

The acute toxicities of selected drugs were assayed biochemically in vitro by estimation of LC50 dose in human lymphocyte cultures. Two methods were compared for estimation of LC50 values, the redistribution of DNA and lactate dehydrogenase after treatment with various concentrations of the compound. Ten of the chemicals used had previously been tested in HeLa cells using morphological changes as the endpoint. The LC50 values found in this study were a little lower than the IC50 values for HeLa cells. The enzyme assay gave lower LC50 values than the DNA assay, but the differences were not significant. LC50 values were also found for two drugs tested in several cell lines with redistribution of DNA and proteins. Again, no significant differences were apparent.

Keywords

LC 50 human lymphocytes redistribution of DNA lactate dehydrogenase

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Estimation of LC50 Values by Assay of Lactate Dehydrogenase and DNA Redistribution in Human Lymphocyte Cultures

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