Sage Journals: Discover world-class research

Abstract

Hyaline glomerulopathy is a spontaneous disease of undetermined etiology that occurs sporadically in various strains of aging mice. In our laboratory, this disease was observed with unusual ultrastructural features as an incidental finding in 2 female B6C3F1 mice from 2 carcinogenicity bioassays. Microscopically, renal lesions were characterized by marked diffuse enlargement and prominent hyalinization of the glomeruli, equally affecting both kidneys. Affected glomeruli were PAS positive, but were negative for amyloid by the Congo red method. Immunocytochemical staining revealed weakly positive glomerular deposits with polyclonal anti-mouse IgG-IgM-IgA cocktail. Ultrastructurally, there were characteristic subendothelial osmio-philic deposits composed of loosely-packed linear structures in the glomeruli. Lamellae, which appeared as fibrils in perpendicular sections, were relatively uniform, measured 6.1-17.01 nm in diameter, and formed single or double-layered structures. The ultrastructural and immunocytochemical characteristics are suggestive of a spontaneous immune-mediated mechanism in a strain of mouse commonly used in toxicology studies.

Keywords

Hyaline glomerulopathy ultrastructure lamellae kidney mouse

References

1. Churg J and Grishman E (1972). Ultrastructure of immune deposits in renal glomeruli. Ann. Int. Med. 76: 479–486

2. Duffy JL , Khurana E , Susin M , Gomez-Leon G , and Churg J (1983). Fibrillary renal deposits and nephritis. Am. J. Pathol. 113: 279–290.

3. Dunn TB (1949). Some observations on the normal and pathologic anatomy of the kidney of the mouse. J. Natl. Cancer Inst. 9: 285–301.

4. Dunn TB and Larsen CD (1946). Hyalinization of glomeruli produced in strain A mice by administration of urethane (ethyl carbamate). Fed. Proc. 5: 220.

5. Ghadially FN (1988). Ultrastructural Pathology of the Cell and Matrix, 3rd ed. Butterworths, London, pp. 1060–1063.

6. Grishman E , Porush JC , Rosen SM , and Churg J (1967). Lupus nephritis with organized deposits in the kidneys. Lab. Invest. 16: 717–725.

7. Gude WD and Lupton AC (1960). Spontaneous glomerulosclerosis in aging RF mice. J. Gerontol. 15: 373–376.

8. Guttman PH and Kohn HI (1960). Progressive in-tercapillary glomerulosclerosis in the mouse, rat and Chinese hamster associated with aging and x-ray exposure. Am. J. Pathol. 37: 293–307.

9. Hirokawa K (1975). Characterization of age-associated kidney disease in Wistar rats. Mech. Ageing Dev. 4: 301–316.

10.

10. Hsu S-M , Raine L , and Fanger H (1981). Use of avidin-biotin-peroxidase complex (ABC) in itnmu-noperoxidase techniques: A comparison between ABC and unlabelled antibody (PAP) procedures. J. His-tochem. Cytochem. 29: 577–580.

11.

11. Kirschbaum A (1944). Spontaneous glomerulonephritis in mice. Proc. Soc. Exper. Biol. Med. 55: 280–281.

12.

12. Linder E , Pasternack A , and Edgington TS (1972). Pathology and immunology of age-associated disease of mice and evidence for an autologous immune complex pathogenesis of the associated renal disease. Clin. Immunol. Immunopathol. 1: 104–121.

13.

13. Manligod JR , Pirani CL , Miyasato F , and Pollak VE (1967). The renal changes in NZB/B1 and NZB-NZW F1 hybrid mice. Light and electron microscopic studies. Nephron 4: 215–230.

14.

14. McGiven AR and Lynraven GS (1968). Glomerular lesions in NZB/NZW mice. Electron microscopic study of development. Arch. Pathol. 85: 250–261.

15.

15. Pelletier M , Hinglais N , and Bach JF (1975). Characteristic immunohistochemical and ultrastructural glomerular lesions in Nude mice. Lab. Invest. 32: 388–396.

16.

16. Peter CP (1973). Possible immune origin of age-related pathological changes in long-lived mice. J. Gerontol. 28: 265–275.

17.

17. Sass B (1986). Glomerulonephritis, mouse. In: Urinary System, TC Jones , U Mohr , and RD Hunt (eds). Springer-Verlag, New York, pp. 192–210.

18.

18. Shimamura T and Sorenson GD (1965). Electron microscopy of serial sections of the murine glomerular mesangium. Anat. Rec. 152: 141–154.

19.

19. Silva FG and Pirani CL (1988). Electron microscopic study of medical diseases of the kidney: Update— 1988. Mod. Pathol. 1: 292–315.

20.

20. Treser G , Oppermann W , Ehrenreich T , Lange K , Levine R , and Camerini-Davalos RA (1968). Glomerular lesions in a strain of genetically diabetic mice. Proc. Soc. Exp. Biol. Med. 129: 820–823.

Hyaline Glomerulopathy in B6C3F1 Mice

Abstract

Keywords

References