Abstract
Electrical stimulation was applied to bull carcasses at a low voltage (80 V, 15 Hz, 30 s). The pH decline, tenderness, myofibrillar protein degradation, and exoprotease activities were studied in semimembranosus and longissimus dorsi muscles during the ageing process. Electrical stimulation was found to be beneficial for tenderness in longissimus dorsi muscle as determined by shear force and confirmed by the earlier appearance of the 30-kDa band in the electrically stimulated muscles. The acceleration of the glycolytic process after electrical stimulation affected exoprotease activities, in turn induced the activation of dipeptidyl peptidases I and II and the inhibition of methionyl, leucyl and pyroglutamyl aminopeptidases; however electrical stimulation did not influence alanyl aminopeptidase and arginyl aminopeptidase.
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