Introduction
The migration of intravenously transplanted human neural stem cells (hNSCs) in ischemic rats was investigated.
Methods
hNSCs were colabeled with Endorem and BrdU, and then the hNSCs (4×106 cells) were intravenously injected in ischemic rats. After injection, rats were sacrificed at different time points (3 hr, 6 hr, 1 d, 3 d, 5 d, 7 d, 14 d and 21 d) and each rat brain and six organs (liver, lung, heart, intestine, kidney, spleen) were isolated, followed by histological analysis.
Results
On 3 d after transplatation, hNSCs were detected in the focal ischemia lesion of rat brain and remained for 21 d whereas they were not observed in any other organs that were tested. However, hNSCs were detected in the spleen at entire time points.
Conclusion
Our study demonstrates that the intravenously transplanted hNSCs migrated specifically into ischemia lesion with the exception of the spleen.
Footnotes
Acknowledgements
Grant support: This study was supported by a grant of the Korea Health 21 R & D Project, Ministry of Health & Welfare, Republic of Korea. (Project No.: 0405-B002-0205-0001)