The effect of iodoacetate (IAA), an inhibitor of glycolysis, on local CBF (LCBF) and local CMRglu (LCMRglu) was studied in cats by means of a double-radionuclide autoradiographic method. Artificial CSF containing 5 mM IAA was superfused on the left parietal cortex under a cranial window for 30 min. [14C]2-Deoxyglucose and [123I]iodoantipyrine were injected for the determination of LCMRglu and LCBF, respectively. A marked increase in LCBF, accompanied by a moderate to severe depression of LCMRglu, was observed in the IAA-superfused cortex. This result suggests that LCBF may be closely regulated by the cellular energy state associated with glycolytic activity in brain tissue.
Abdul-RahmanASiesjöBK, (1980) Local cerebral glucose consumption during insulin-induced hypoglycemia, and in the recovery period following glucose administration. Acta Physiol Scand110:149–159
2.
Abdul-RahmanAAgardhCDSiesjöBK, (1980) Local cerebral blood flow in the rat during severe hypoglycemia, and in the recovery period following glucose injection. Acta Physiol Scand109:307–314
3.
DoraE, (1983) Glycolysis and epilepsy-induced changes in cerebrocortical NAD/NADH redox state. J Neurochem41:1774–1777
4.
DoraE, (1984) A single cranial window technique for optical monitoring of cerebrocortical microcirculation and NAD/NADH redox state. Effect of mitochondrial electron transport inhibitors and anoxic anoxia. J Neurochem42:101–108
5.
DoraEKovachAGB, (1983) Effect of topically administered epinephrine, norepinephrine, and acetylcholine on cerebrocortical circulation and the NAD/NADH redox state. J Cereb Blood Flow Metabol3:161–169
6.
DoraEKovachAGB, (1984) Glycolysis and regulation of cerebral blood flow and metabolism. In Oxygen Transport to Tissue (LubbersDWAckerHGoldstickTKLeniger-FollertE, eds), New York, Plenum Press, pp. 305–314
HealdPJ, (1953) The effect of metabolic inhibitors on respiration and glycolysis of electrically stimulated cerebral-cortex slices. Biochem J55:625–631
9.
JonesSCFedoraTLearJGreenbergJHReivichM, (1981) The flow-metabolism couple in normal rat brain. J Cereb Blood Flow Metabol1 (suppl 1):S488–S499
10.
KennedyCDesRosiersMHSakuradaOShinoharaMReivichMJehleJWSokoloffL, (1976) Metabolic mapping of the primary visual system of the monkey by means of the autoradiographic [14C]deoxyglucose technique. Proc Natl Acad Sci USA73:4230–4234
11.
LearJLJonesSCGreenbergJHFedoraTJReivichM, (1981) Use of 123-I and 14-C in a double radionuclide autoradiographic technique for simultaneous measurement of LCBF and LCMRglu. Theory and method. Stroke12:589–597
12.
LowryOHPassonneauJV, (1972) A Flexible System of Enzymatic Analysis. New York, Academic Press
13.
NilssonBAgardhCDIngvarMSiesjöBK, (1981) Cerebrovascular response during and following severe insulin-induced hypoglycemia: CO2-sensitivity, autoregulation, and influence of prostaglandin synthesis inhibition. Acta Physiol Scand111:455–463
14.
NuutinenEMNishikiKErecinskaMWilsonDF, (1982) Role of mitochondrial oxidative phosphorylation in regulation of coronary blood flow. Am J Physiol243:H159–H169
15.
PurvesMJ, (1972) The Physiology of the Cerebral Circulation. New York, Cambridge University Press
16.
ReivichM, (1974) Blood flow metabolism couple in brain. In: Association for Research in Nervous and Mental Disease, Vol 53: Brain Dysfunction in Metabolic Disorders (PlumF, ed), New York, Raven Press, pp. 125–140
17.
ReivichMJehleJSokoloffLKetySS, (1969) Measurement of regional cerebral blood flow with antipyrine-14-C in awake cats. J Appl Physiol27:296–300
18.
RoyCSSherringtonMB, (1890) On the regulation of blood-supply of the brain. J Physiol11:85–108
19.
SakuradaOKennedyCJehleJBrownJDCarbinGLSokoloffL, (1978) Measurement of local cerebral blood flow with iodo[14-C]antipyrine. Am J Physiol234:H59–H66
20.
SavakiHEDavidsonLSmithLSokoloffL, (1980) Measurement of free glucose turnover in brain. J Neurochem35:495–502
21.
ShinoharaMDollingerBBrownGRapoportSSokoloffL, (1979) Cerebral glucose utilization: Local changes during and after recovery from spreading cortical depression. Science203:188–203
22.
SickTJRosenthalMLaMannaJCLutzPL, (1982) Brain potassium ion homeostasis, anoxia, and metabolic inhibition in turtle and rats. Am J Physiol243:R281–R288
23.
SokoloffL, (1981) Relationship among local functional activity, energy metabolism, and blood flow in the central nervous system. Fed Proc40:2311–2316
24.
SokoloffLReivichMKennedyCDesRosiersMHPatlakCSPettigrewKLSakuradaOShinoharaM, (1977) The [14C]-deoxyglucose method for the measurement of local cerebral glucose utilization: Theory, procedure, and normal values in the conscious and anesthetized albino rat. J Neurochem28:897–916
25.
TodaN, (1984) Calcium independent contraction induced by iodoacetic acid in isolated cerebral arteries. Stroke15:475–480
26.
WahlMKuschinskyW, (1976) The dilatory action of adenosine on pial arteries of cats and its inhibition by theophylline. Pflugers Arch362:55–59
27.
WebbJL, (1966) Iodoacetate and iodoacetamide. In: Enzyme and Metabolic Inhibitors, Vol 3: Iodoacetate, Maleate, N-Ethylmaleimide, Alloxan, Quinines, Arsenicals (WebbJL, ed), New York, Academic Press, pp. 1–283
28.
WelshFARiederW, (1978) Evaluation of in situ freezing of cat brain by NADH fluorescence. J Neurochem31:299–309
29.
WelshFAO'ConnorMJMarcyVRSpataccoAJJohnsRL, (1982) Factors limiting regeneration of ATP following temporary ischemia in cat brain. Stroke13:234–242
