The presence of immunoreactive glucagon (IRG) in smooth muscle of the cerebral arteries of rats was demonstrated immunohistochemically using two antisera against pancreatic glucagon, OAL-123 and Unger's 30K. Based on the results and on our previous radioimmunoassay and gel filtration study, the smooth muscle cells of the blood vessels may be one of the extrapancreatic sources of IRG in the plasma.
CoonsAH, (1958) Fluorescent antibody methods. In: General Cytochemical Methods (DanielliJF, ed), New York, Raven Press, pp 399–422
2.
EdvinssonLFahrenkrugJHankoJOwmanCSundlerFUddmanR, (1980) VIP (vasoactive intestinal polypeptide)-containing nerves of intracranial arteries in mammals. Cell Tissue Res208:135–142
3.
EdvinssonLMcCullochJUddmanR, (1981) Perivascular localization and cerebrovascular effects of substance P. J Cereb Blood Flow Metabol1(suppl 1):S319–S320
4.
EdvinssonLMcCullochJTatemotoKUddmanR, (1983) Neuropeptide Y: A unique system of perivascular nerve mediating contraction of cerebral vessels. J Cereb Blood Flow Metabol3(suppl 1):S182–S183
5.
ImagawaKNishinoTShinSUeharaSHashimuraEYanaiharaCYanaiharaN, (1979) Production of anti-glucagon sera with a C-terminal fragment of pancreatic glucagon. Endocrinol Jpn26:123–131
6.
LarssonLIEdvinssonLFahrenkrugJHåkansonROwmanCde MuckadellOB SchffalitzkeySundlerF, (1976) Immunohistochemical localization of a vasodilatory polypeptide (VIP) in cerebrovascular nerves. Brain Res113:400–404
7.
LeeTHumeWRSuCBevanJ, (1978) Neurogenic vasodilation of cat cerebral arteries. Circ Res42:535–542
8.
MatsuyamaTMatsumotoMShiosakaSFujisawaAYonedaSKimuraKAbeHTohyamaM, (1983a) Overall distribution of substance P and vasoactive intestinal polypeptide in the cerebral arteries: An immunohistochemical study using whole mount. J Cereb Blood Flow Metabol3(suppl 1):S208–S209
9.
MatsuyamaTShiosakaSMatsumotoMYonedaSKimuraKAbeHHayakawaTInoueHTohyamaM, (1983b) Overall distribution of vasoactive intestinal polypeptide-containing nerves on the wall of cerebral arteries: An immunohistochemical study using whole-mounts. Neuroscience10:89–96
10.
TanakaJShiosakaSTsubouchiHGohdaEDaikuharaYTohyamaM, (1983) Immunoreactive glucagon in the vascular walls of the rat. Life Sci33:1549–1554
YamamotoKMatsuyamaTShiosakaSInagakiSSenbaEShimizuYIshimotoIHayakawaTMatsumotoMTohyamaM, (1983) Overall distribution of substance P-containing nerves in the wall of the cerebral arteries of the guinea pig and its origins. J Comp Neurol215:421–426
13.
ZamboniLDe MartinoC, (1967) Buffered picric-acid formaldehyde: A new rapid fixative for electron microscopy. J Cell Biol35:148A
