Abstract
Martina Raudenska, Marketa Sztalmachova, Jaromir Gumulec, Michaela Fojtu, Hana Polanska, Jan Balvan, Marek Feith, Hana Binkova, Zuzana Horakova, Rom Kostrica, Rene Kizek and Michal Masarik
[Tumor Biology,
The section Preparation of tissue samples contained a mistake. The tumour-adjacent tissue in this study was not verified histologically, but only macroscopically by the surgeon. The main message of this study stays bona fide the same. The correct version of tissue samples preparation is listed here:
Preparation of tissue samples
The study was conducted in accord with the Helsinki Declaration of 1964 and all subsequent revisions thereof. It was approved by the ethical committee of St. Anne’s Faculty Hospital, Brno, Czech Republic. All surgical tissue samples were obtained from HNSCC patients after they signed informed consent. Tumour tissue (T) was separated by the surgeon and the adjoining cut was evaluated by the pathologist to verify squamous cell carcinoma histology. The rest of the tumour tissue was placed into RNAlater. The tumour-adjacent tissue (A) was a matched sample of non-tumorous mucosa adjacent to the tumour site which was macroscopically healthy, i.e. at a safe distance from the tumour. Available tissues from a noncancerous tonsillectomy (Tons) were also collected. The tissue material harvested at surgery was placed into RNAlater Solution for RNA stabilisation and storage (Ambion, Carlsbad, CA, USA). The material was maintained cold, and RNA was isolated within 24 h.