Effect of beta adrenergic agents on granulocyte deformability

Abstract

The effect of beta adrenergic agents on granulocyte deformability was investigated using the filtration technique. Three groups of granulocytes were studied: 1) cells treated with isoproterenol (10⁻⁶ to 10⁻³ M); 2) cells treated with propranolol (10⁻⁶ to 10⁻³ M); and 3) control cells. Granulocytes were pumped at a constant rate through polycarbonate filters with 5 µm diameter pores. The pressure generated as the cell suspension was pumped through the filter reflected the deformability of the cells. Isoproterenol in concentrations ranging between 10⁻⁶ to 10⁻³ M and propranolol in concentrations < 3.5 × 10⁻⁴ M did not change the filtration pressure of granulocytes. Propranolol in concentrations ≥ 3.5 × 10⁻⁴ M increased the filtration pressure. This decrease in deformability of granulocytes was associated with a loss of pseudopod formation and a decrease in percent viability. High doses of propranolol decrease granulocyte deformability.