Effects of endurance training on hsa-miR-223,P2RY 12 receptor expression and platelet function in type 2 diabetic patients

Abstract

BACKGROUND:

Type 2 diabetes mellitus (T2DM) is associated with thrombotic events due to platelets’ defects.

OBJECTIVE:

The aim of this study was to investigate the effects of endurance training on expressions of platelet hsa-miR-223 and P2RY₁₂ receptor, as well as platelet function in T2DM patients.

METHODS:

Twenty female T2DM patients (age, 62.25±3.81 yr; weight, 73.53±9.04 kg; height, 154.7±4.3 cm) were randomly divided into two groups (Control (CONT) and endurance training (ET)). Subjects in ET group performed eight weeks of running on treadmill at 60–75% of VO₂peak (moderate intensity), 3 sessions per week, while, the subjects in control group were not involved in any kind of exercise training. Two blood samples were taken before and 48 h after the training and were analyzed for platelet aggregation, and hsa-miR-223 and P2RY₁₂ expressions.

RESULTS:

Although platelet aggregation decreased significantly in ET group (P <  0.05), these changes were not significantly different between two groups. Expression of platelet hsa-miR-223 increased and P2RY₁₂ mRNA reduced following ET non-significantly. However, decreases in fasting blood glucose, glycated hemoglobin and body weight, and increases in VO₂peak following ET were significantly different when compared to control group (P <  0.05).

CONCLUSIONS:

Short-term endurance training dose not induce up-regulation of hsa-miR-223 and down-regulation of P2RY12, while it has a positive impact on platelet function, glycemic indices, physical fitness and body composition in female T2DM patients.