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Abstract

Preparations containing RNA inactivator(s) synthesized from Cu²⁺ and hydroquinone were tested for their effect on monoribonucleotides. The preparations caused the disappearance of large fractions of all of the monoribonucleotides tested, which were the 3′-ribonucleotides and the 5-ribonucleotides of adenine, cytosine, guanine, and uracil, and an impurity identified as the 2′-ribonucleotide of uracil. Control preparations with Cu²⁺ but without hydroquinone had no effect on the ribonucleotides. Control preparations with hydroquinone but without deliberately added Cu²⁺ caused the disappearance of relatively small fractions of the ribonucleotides tested. The activity of these control preparations is probably attributable, at least in part, to trace copper impurity or contamination. The addition of L-histidine protected the ribonucleotides against the effect of the RNA inactivator preparations from Cu²⁺ and hydroquinone, just as the L-histidine protects naked viral RNA against the RNA inactivator(s). The hypothesis that the ribonucleotide moieties of the viral RNA are the targets of the RNA inactivator(s) is discussed.

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The Effect of RNA Inactivator(s) Synthesized from Cu 2+ and Hydroquinone on Monoribonucleotides

Abstract

References