Intraoperative Imaging of Positron Emission Tomographic Radiotracers Using Cerenkov Luminescence Emissions

Figure S1.

Control Cerenkov luminescence imaging (CLIs) of mice 1 to 8 at 144 hours postinjection covered with optically dense black paper. This image confirms that the CLI signal observed is not due to the interference of γ-rays with the charge-coupled device detector.

Table S1.

Optical ROI Data from CLI of ⁸⁹Zr-DFO-Trastumab Uptake in Mice 1 to 8 Bearing BT-474, MDA-MB-468, BT-474 Block, and MDA-MB-468 Block Tumors

	Normal Mice 1–4 89Zr-DFO-Trastuzumab (23–24 μg mAb)		Block Mice 5–8 89Zr-DFO-Trastuzumab (125 μg mAb)
Time	MDA-MB-468	BT-474	MDA-MB-468	BT-474
Average radiance/(p/s/cm2/sr)
3	1,285 ± 432	3,053 ± 871	381 ± 464	1,738 ± 744
24	4,516 ± 1,355	9,905 ± 1,857	3,530 ± 1,186	3,611 ± 1,381
48	4,261 ± 930	9,760 ± 3,413	4,268 ± 1,339	4,894 ± 1,048
72	5,047 ± 1,636	12,727 ± 2,920	5,451 ± 1,517	7,000 ± 986
96	3,791 ± 1,611	9,608 ± 3,680	4,998 ± 1,635	6,418 ± 1,077
120	5,236 ± 1,963	11,568 ± 4,800	5,747 ± 2,014	6,450 ± 774
144	4,469 ± 1,503	10,619 ± 3,031	5,932 ± 2,066	7,902 ± 645
Maximum radiance/(p/s/cm2/sr)
3	1,869 ± 1,097	7,354 ± 2,834	3,579 ± 3,450	9,484 ± 2,776
24	5,656 ± 1,817	18,177 ± 3,547	6,538 ± 3,213	5,797 ± 2,345
48	6,277 ± 2,290	21,683 ± 6,295	5,536 ± 1,200	8,187 ± 2,166
72	5,174 ± 2,821	26,779 ± 7,305	8,260 ± 2,935	11,118 ± 1,938
96	7,996 ± 8,278	22,602 ± 7,326	5,817 ± 2,209	10,011 ± 1,111
120	6,671 ± 3,604	22,961 ± 6,362	8,324 ± 3,190	10,484 ± 991
144	6,499 ± 3,816	25,578 ± 9,857	9,302 ± 2,547	12,239 ± 3,485

CLI = Cerenkov luminescence imaging; mAb = monoclonal antibody; ROI = region of interest.

Table S2.

PET VOI Data of ⁸⁹Zr-DFO-Trastumab Uptake in Mice (1–8) Bearing BT-474, MDA-MB-468, BT-474 Block, and MDA-MB-468 Block Tumors

	Normal Mice 1–4 89Zr-DFO-Trastuzumab (23–24 μg mAb)		Block Mice 5–8 89Zr-DFO-Trastuzumab (125 μg mAb)
Time	MDA-MB-468	BT-474	MDA-MB-468	BT-474
Mean %ID/g
3	2.9 ± 1.1	5.3 ± 1.6	3.1 ± 1.2	2.9 ± 0.7
24	3.3 ± 1.5	9.4 ± 2.6	3.7 ± 1.0	4.4 ± 1.0
48	3.8 ± 0.8	8.9 ± 2.0	3.8 ± 0.6	4.3 ± 0.7
96	3.8 ± 0.8	8.5 ± 2.7	4.0 ± 0.5	4.7 ± 0.3
144	3.7 ± 0.7	7.4 ± 1.6	3.6 ± 1.1	4.3 ± 0.5
Maximum %ID/g
3	4.9 ± 1.6	10.1 ± 3.3	5.8 ± 2.3	5.2 ± 1.9
24	6.5 ± 1.6	21.0 ± 5.9	6.2 ± 1.5	9.2 ± 2.7
48	7.1 ± 1.6	25.3 ± 7.8	7.1 ± 1.6	11.2 ± 3.4
96	6.9 ± 1.6	30.0 ± 11.5	7.9 ± 1.3	12.0 ± 2.6
144	7.2 ± 0.9	28.7 ± 10.6	7.8 ± 2.5	12.5 ± 3.4

%ID/g = percent injected dose per gram; mAb = monoclonal antibody; PET = positron emission tomography; VOI = volume of interest.

Table S3.

t-Test Analysis of the Statistical Significance between Radiotracer Uptake in Four Tumor Types (BT-474, MDA-MB-468, BT-474 Block, and MDA-MB-468 Block) as Measured by CLI Using Maximum and Average Radiance*

Data Set	Time (h)
A	B	3	24	48	72	96	120	144
Maximum radiance
BT-474	MDA-MB-468	0.0238	0.0022	0.0115	0.0058	0.0389	0.0075	0.0238
BT-474	BT-474 block	0.9438	0.0018	0.0180	0.0197	0.0397	0.0279	0.0675
BT-474	MDA-MB-468 block	0.5922	0.0029	0.0127	0.0096	0.0154	0.0120	0.0390
MDA-MB-468	MDA-MB-468 block	0.1447	0.6538	0.5936	0.1803	0.6419	0.5181	0.3235
BT-474 block	MDA-MB-468	0.1212	0.9274	0.2713	0.0161	0.6614	0.1220	0.0684
BT-474 block	MDA-MB-468 block	0.7192	0.7238	0.0889	0.1629	0.0234	0.2733	0.2821
Average radiance
BT-474	MDA-MB-468	0.0188	0.0042	0.0440	0.0068	0.0429	0.0714	0.0188
BT-474	BT-474 block	0.0626	0.0021	0.0598	0.0238	0.1813	0.1214	0.1701
BT-474	MDA-MB-468 block	0.0038	0.0020	0.0414	0.0087	0.0817	0.0885	0.0483
MDA-MB-468	MDA-MB-468 block	0.0292	0.3162	0.9936	0.7297	0.3333	0.7290	0.2995
BT-474 block	MDA-MB-468	0.3418	0.3860	0.4013	0.0970	0.0403	0.3157	0.0132
BT-474 block	MDA-MB-468 block	0.0268	0.9315	0.4906	0.1457	0.2047	0.5514	0.1512

CLI = Cerenkov luminescence imaging.

*

Values of p < .05 are highlighted in boldface.

Table S4.

Complete Biodistribution Data for ⁸⁹Zr-DFO-Trastuzumab Uptake in BT-474 and MDA-MB-468 Tumor–Bearing Mice at 144 Hours Postadministration

	Tissue Uptake/%ID/g
Organ	144 h (n = 4) Normal Mice 1–4	144 h (n = 4) Block Mice 5–8
Blood	8.7 ± 0.6	8.6 ± 1.3
Tumor (BT-474)	47.2 ± 6.2	21.9 ± 7.5
Tumor (MDA-MB-468)	7.2 ± 1.3	6.5 ± 0.4
Heart	3.1 ± 0.8	4.1 ± 1.1
Lungs	5.3 ± 0.7	4.9 ± 1.3
Liver	6.4 ± 0.1	6.4 ± 0.7
Spleen	7.8 ± 1.3	6.7 ± 1.3
Stomach	0.4 ± 0.1	0.3 ± 0.1
Small intestine	0.9 ± 0.2	1.0 ± 0.2
Large intestine	0.5 ± 0.0	0.6 ± 0.1
Kidney	3.9 ± 0.3	4.9 ± 1.8
Muscle	1.1 ± 0.7	0.7 ± 0.1
Bone	3.0 ± 0.7	2.2 ± 0.5

%ID/g = percent injected dose per gram.