Brain function is an extremely active dynamic process. Studing a living organism is essential for explaination of the sophistication of the brain. Although there are progresses in studying living tissues of acute or cultured slice for prolonged period in vitro, clinicians prefer seeing the lesion directly on an imaging screen or material. Functional MRI (fMRI) is commonly used method to study brain pathology in dynamics.
References
1.
AndersonP., MorrisR., AmaralD., BlissT., O'KeefeJ.Historical perspective: Proposed functions, biological characteristics, and neurobiological models of the hippocampus. In The Hippocampus Book.AndersonP., MorrisR., AmaralD., BlissT., O'KeefeJ., Eds. Oxford: Oxford University Press, 2006, pp 9–36.
2.
LeinP.J., BarnhartC.D., PessahI.N.Acute hippocampal slice preparation and hippocampal slice cultures. In In Vitro Neurotoxicology.CostaL.G., GiordanoG., GuizzettiM., Eds. Totowa, NJ: Humana Press, 2011, pp 115–134.
3.
LoD.C., McAllisterA.K., KatzL.C.Neuronal transfection in brain slices using particle-mediated gene transfer. Neuron1994, 13(6): 1263–1268.