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Abstract

This report describes a new application of surface-enhanced Raman spectroscopy (SERS) in the analysis of highly complex and crude biomedical samples. The specific cases examined are ophthalmic extracts obtained from the homogenization of a normal human lens, a senile cataractous human lens, and a chipmunk lens. The results demonstrate that SERS is capable of selectively probing certain biological components with high mass sensitivity. In the chipmunk and normal human lens extracts, adenine-containing molecules (e.g., adenosine-5'-monophosphate) are found to be abundantly present, while intense tyrosine and tryptophan Raman signals are apparent in the SERS spectrum of the cataractous human lens sample. We have also conducted gel-filtration and mass spectrometric studies of the eye lens extracts, and the results indicate that SERS is an effective method for biomedical species identification.

Keywords

SERS Raman Eye lens Ocular pigments Cataract

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Surface-Enhanced Raman Analysis of Biomedical Eye Lens Extracts

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