Secretion granules in the shell gland, isthmus, and albumin-secreting region of the hen oviduct were analyzed with WET-scanning electron microscopy (SEM) and EDX, a combination of wide-angle backscattered electron detector (BED) and energy-dispersive X-ray microanalyzer (EDX). Glutaraldehyde-fixed but unhydrated, unstained, and uncoated samples were analyzed; Ca was localized in all secretion granules in all three sections of the hen oviduct studied.
GayCV, SchraerH: Autoradiographic localization of calcium in the mucosal cells of the avian oviduct. Calcif Tissue Res7: 201, 1971
2.
MakitaT, NishidaS: The fine structure of the avian oviduct. Proceedings 6th International Congress Electron Microscopy, vol. 2. 1966, p 777–778
3.
MakitaT, SandbornEB: Scanning electron microscopy of secretion granules in albumin gland cells of the laying hen oviduct. Exp Cell Res60: 477, 1970
4.
MakitaT, SandbornEB: Identification of intracellular components by scanning electron microscopy. Exp Cell Res67: 211, 1971
5.
MakitaT, KiwakiS, SandbornEB: Secretion granules in the ciliated cells of the avian oviduct. Arch Histol Jpn35: 209, 1973
6.
MakitaT: X-ray microanalysis of the avian shell gland and egg shell. Scan Electron Microsc2: 473, 1981
7.
MakitaT, HatsuokaM: Scanning electron microscopy and X-ray microanalysis of shell membrane of avian egg and secretion granules in the isthmus of hen oviduct. Scan Electron Microsc2, in press
8.
MakitaT, UedaH, IdegomoriT: Applications of WET-SEM for X-ray microanalysis of hydrated biological specimens. Scan Electron Microsc2, in press
9.
RobinsonVNE: The construction and uses of an efficient back-scattered electron detector for SEM. J Phys Ed Sci Instrum7: 650, 1974
10.
RobinsonVNE: Backscattered electron imaging. Scan Electron Microsc51, 1975
11.
SchraerR, SchraerH: Changes in metal distribution of the avian oviduct during the ovulation cycle. Proc Soc Exp Biol Med119: 937, 1965
