MODY5 Hepatocyte Nuclear Factor 1ß (HNF1ß)-Associated Nephropathy: experience from a regional monogenic diabetes referral centre in Singapore

Abstract

From our monogenic diabetes registry set-up at a secondary-care diabetes center, we identified a nontrivial subpopulation (~15%) of maturity-onset diabetes of the young (MODY) among people with young-onset diabetes. In this report, we describe the diagnostic caveats, clinical features and long-term renal-trajectory of people with HNF1B mutations (HNF1B-MODY). Between 2013 and 2020, we received 267 referrals to evaluate MODY from endocrinologists in both public and private practice. Every participant was subjected to a previously reported structured evaluation process, high-throughput nucleotide sequencing and gene-dosage analysis. Out of 40 individuals with confirmed MODY, 4 (10%) had HNF1B-MODY (harboring either a HNF1B whole-gene deletion or duplication). Postsequencing follow-up biochemical and radiological evaluations revealed the known HNF1B-MODY associated systemic-features, such as transaminitis and structural renal-lesions. These anomalies could have been missed without prior knowledge of the nucleotide-sequencing results. Interestingly, preliminary longitudinal observation (up to 15 years) suggested possibly 2 distinct patterns of renal-deterioration (albuminuric vs. nonalbuminuric chronic kidney disease). Monogenic diabetes like HNF1B-MODY may be missed among young-onset diabetes in a resource-limited routine-care clinic. Collaboration with a MODY-evaluation center may fill the care-gap. The long-term renal-trajectories of HNF1B-MODY will require further studies by dedicated registries and international consortium.

Keywords

HNF1B-associated nephropathy HNF1B-MODY MODY5 renal anomalies renal trajectory

Introduction

Mutations in the hepatocyte nuclear factor-1B (HNF1B) gene was first reported in 1997 as a rare genetic cause of monogenic diabetes or maturity-onset diabetes of the young (MODY) by Horikawa Y et al.¹ MODY is characterized by young-onset diabetes (typically <30 years old), lean, absence of beta cell autoimmunity, noninsulin dependence, and nonketotic prone hyperglycemia.² It is a form of Mendelian dominantly inherited disease primarily caused by underlying genetic defects in more than 20 different genes identified to date,³ including the HNF1B gene.

Genetic defects in HNF1B account for 2% to 6% of all MODY cases and affected individuals usually present with extra-renal features, including multicystic dysplastic kidneys, glomerulocystic kidney disease, renal agenesis, renal hypoplasia, and renal interstitial fibrosis.^4,5 HNF1B belongs to the homeobox-containing family of transcription factors and plays an essential role in the development and function of epithelia in the pancreas, kidneys, liver, and genitourinary tract.⁵ Till date, more than 50 different variants have been described within the HNF1B gene,⁶ located on chromosome 17q12. The most common genetic defect, occurring in 50% of HNF1B-MODY patients, is a whole-gene deletion. In fact, virtually all HNF1B whole-gene deletions have been associated with the 17q12 recurrent deletion syndrome (due to a 1.4 Mb heterozygous recurrent deletion at chromosome 17q12 which contains 15 genes, including HNF1B).^7,8 This syndrome is characterized by variable combinations of clinical features including young-onset diabetes (MODY), neurodevelopmental, or neuropsychiatric disorders (ie, developmental delay, intellectual disability, autism spectrum disorder, schizophrenia, anxiety, and bipolar disorder), structural lesion of the intra-abdominal organs involving the pancreas and genital-tract, renal tubule-interstitial disease, and congenital abnormalities of the kidney and urinary tract (CAKUT).⁹ The recently proposed HNF1B risk score¹⁰ is a useful discriminative tool which relies on a combination of clinical, imaging and biological variables, in selecting patients for HNF1B genetic testing. However, a crucial determinant of this risk score is to radiologically detect intra-abdominal organ defects, which may not be routinely ordered without prior suspicion, even in specialist diabetes clinics, thereby limiting the adoption of the scoring system even among diabetes care-providers.

HNF1B-MODY is uniquely susceptible to chronic kidney disease (CKD). This is because of the dual vulnerability conferred by CAKUT and early-onset diabetes. Importantly, the heterogeneity and longitudinal renal trajectory of HNF1B-associated nephropathy has not been well described. In this report, we describe the clinical presentation, long-term renal outcomes (up to 15 years) and therapeutic management of 4 selected HNF1B-MODY patients harboring either a HNF1B whole-gene deletion or duplication. Notably, 2 out of 4 patients, receiving care at a secondary-care diabetes center, were referred for MODY candidate genes nucleotide-sequencing primarily because of atypical diabetes, instead of known CAKUT. Therefore, the HNF1B risk score was not applied prior to gene sequencing.

Materials and Methods

Participants were selected for MODY evaluation based on previously described clinical criteria.^11,12 Briefly, these include age of diabetes onset ≤35 years, body mass index (BMI) <32.5 kg/m² (threshold motivated by the BMI value collected often after long duration of diabetes rather than observed upon diabetes onset), absence of diabetic ketoacidosis (DKA) and negative glutamic acid decarboxylase antibodies (GADA). Massively parallel targeted-gene sequencing for 16 MODY-associated genes and gene dosage analysis using multiplex ligation-dependent probe amplification (MLPA) method combined with MRC-Holland kit P241-E1 for 5 MODY genes (as described by Ang SF et al) were carried out.¹² Chromosomal microarray using whole genome Infinium CytoSNP-850K v1.2 Illumina BeadChip or MLPA using the SALSA MLPA Probemix P297 Microdeletion Syndromes-2 assay (MRC-Holland) was used to detect the recurrent micro-aberration at chromosome 17q12.

Glycated hemoglobin A1c (HbA1c), lipids (cholesterol, triglyceride, LDL-, and HDL-cholesterol), urinary albumin-to-creatinine ratio (uACR) were measured by the American College of Pathology certified Department of Laboratory Medicine at the Khoo Teck Puat Hospital, Singapore. Estimated glomerular filtration rate (eGFR) was calculated from age and serum creatinine using the Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) equation. This study is approved by the Singapore National Healthcare Group Domain Specific Review Board (DSRB Study Reference No. 2011/00232, 2012/01131 and 2017/00276). Informed consent was obtained from all study participants.

Results

Among 267 individuals eligible for genetic testing, 40 (~15%) were diagnosed with MODY. From those with MODY, we identified 4 patients with either a HNF1B whole-gene deletion (NM_000458.4 [HNF1B]: c.195_1868del p.[Met1_Trp557del]) or duplication (NM_000458.4 [HNF1B]: c.195_1868dup p.[Met1_Trp557dup]). Further genetic workup by chromosomal microarray or MLPA suggests a recurrent deletion or duplication at chromosome 17q12 respectively. Table 1 shows the baseline clinical characteristics and key features associated with HNF1B-MODY of all 4 patients upon referral for genetic evaluation. Notably, all 4 patients presented with early-onset diabetes (between 19 and 35 years old) and fairly optimal glucose control (HbA1c between 6.6% and 7.3%; 49 to 56 mmol/mol) at point of referral. Among those with family history available (3 out of 4), all had either first or second degree family history of diabetes. Of note, HNF1B-associated features, especially those that are not routinely measured or performed in a diabetes clinic (eg magnesium concentration, parathyroid hormone, hypo-plastic pancreas, and genital abnormalities), were mostly not assessed before genetic diagnosis. Subsequent targeted diagnostic workup by abdominal or renal ultrasound revealed structural abnormalities of the kidney (multicystic dysplastic kidney, unilateral renal agenesis, or multiple cysts) that were not different from previously reported cases.

Table 1.

Evaluation of HNF1B-MODY Associated Clinical Features and Baseline Characteristics of Four Selected Patients Diagnosed With HNF1B-MODY.

Characteristic	Patient 1		Patient 2		Patient 3		Patient 4
Characteristic	Before	After	Before	After	Before	After	Before	After
Evaluation of HNF1B-MODY associated clinical features before and after genetic diagnosis
Renal cysts	Not assessed	No	Yes^a	Yes	Yes^b	Yes	Not assessed	Yes^c
Diabetes	Yes		Yes		Yes		Yes
Reduced renal function	Yes	Yes	No	Yes	No	No	Yes	Yes
CAKUT	Not assessed	Yes^d	No	No	Yes^e	Yes	Not assessed	No
Hyperechogenicity	Not assessed	No	No	No	Yes^f	Yes	Not assessed	Yes^g
Hypomagnesemia	Not assessed	Yes	Not assessed	Yes	Not assessed	Yes	Not assessed
Hyperuricemic/gout	Not assessed	No	Not assessed	No	Not assessed	No	Not assessed	No
Elevated liver enzymes	Not assessed	Yes	Not assessed	No	Not assessed	Yes	Not assessed	No
Exocrine pancreatic disease	No	No	No	No	No	No	No	No
Urinary tract malformation	Not assessed	Yes^h	Not assessed	No	No	No	Not assessed	No
Genital malformations	Not assessed		Not assessed		No		Not assessed
Neurodevelopmental/neuropsychiatric disorder	Not formally assessed		Not formally assessed		No^		Not formally assessed
Hyperparathyroidism	Not assessed	No	Not assessed	No	Not assessed	No	Not assessed	No
Baseline clinical characteristics at point of referral for MODY evaluation
Sex	Female		Male		Male		Male
Family history of diabetes (first and second degree)	Yes		Yes		Yes		Not available
Age at presentation (at diabetologist), year	19		23		29		35
BMI (kg/m²)	26.2		17.2		14.5		22.6
HbA1c (%; mmol/mol)	6.8; 51		7.3; 56		6.6; 49		7.0; 53
Systolic blood pressure (mmHg)	128		123		147		133
Diastolic blood pressure (mmHg)	73		87		101		79
Total cholesterol (mM)	4.44		5.12		5.00		4.68
HDL-C (mM)	1.79		1.56		1.50		1.13
LDL-C (mM)	2.48		3.56		3.28		2.89
Triglycerides (mM)	1.23		0.77		1.26		2.30
CKD-Epi-eGFR (ml/min/1.73 m²)	62		92		111		34.2
Pharmacological treatment
Insulin	Yes		Yes		Yes		No
OHA	Yes		No		No		Yes
HNF1B mutation	Whole-gene deletion		Whole-gene deletion		Whole-gene deletion		Whole-gene duplication
Diagnostic delay	26 years		10 years		10 months		38 years
HNF1B score at presentation¹	4, 10 (re-evaluation)		12, 14 (re-evaluation)		14, 18 (re-evaluation)		4, 16 (re-evaluation)
MODY probability (Shields BM et al)²	8.2%		1.9%		4%		4.6%
MODY probability (Ang SF et al)³	12.9%		51.8%		Not available*		20.6%

Abbreviations: HNF1B-MODY, Hepatocyte Nuclear Factor-1B-maturity-onset diabetes of the young; CAKUT, congenital abnormalities of the kidney and urinary tract; BMI = body mass index; OHA, oral hypoglycemic agents.

Based on HNF1B score developed by Faguer S et al.¹⁰

Based on MODY probability calculator developed by Shields BM et al.¹³

Based on MODY probability calculator developed by Ang SF et al.¹²

Postgenetic diagnosis, abdominal ultrasonography revealed multiple cysts in both kidneys.

Pregenetic diagnosis, renal ultrasonography revealed multiple cysts in the left kidney.

Postgenetic diagnosis, abdominal ultrasonography revealed multiple cysts in the left kidney.

Postgenetic diagnosis, abdominal ultrasonography revealed unilateral renal agenesis of the right kidney.

Pregenetic diagnosis, renal ultrasonography revealed multicystic dysplastic right kidney which preceded diabetes onset.

Pregenetic diagnosis, renal ultrasonography revealed increased echogenicity in the left kidney.

Postgenetic diagnosis, abdominal ultrasonography revealed increased echogenicity in both kidneys.

Postgenetic diagnosis, abdominal ultrasound revealed presence of mild hydronephrosis in the left kidney suggesting distal urinary tract obstruction.

Patient presented with congenital lateral rectus palsy during adolescent.

Unable to derive probability due to insufficient clinical data required by the calculator.

The first patient, a 48-year-old female, was diagnosed with diabetes at age 19 and was referred for MODY evaluation 26 years after diabetes onset. At the point of referral, she was on Metformin as well as multiple daily insulin injections with NPH Insulin and Insulin Actrapid with total daily dose of 64 units/day or 1 unit/kg of short and intermediate-acting insulin therapy. Postgenetic diagnosis, abdominal ultrasonography carried out in this patient revealed unilateral agenesis of the right kidney and the presence of mild hydronephrosis in the left kidney suggested distal urinary tract obstruction. Glycemic control was variable and mostly suboptimal, with HbA1c ranging between 7% and 9.3% (53-78.1 mmol/mol) over several years (Figure 1). After genetic diagnosis in 2017, she had optimal glucose control, with HbA1c between 6.5% and 7% (48 to 53 mmol/mol). Kidney function evaluation suggest CKD based on eGFR of <60 mL/min/1.73 m² for more than 2 years, elevated serum creatinine levels and prolonged microalbuminuria based on uACR between 10 and 60 µg/mg, initially attributed to uncontrolled glycemic burden by the primary physician. Notably, in recent years, her rate of eGFR decline was -2.26 mL/min/1.73 m² per year.

Figure 1.

Longitudinal renal trajectory of Patient 1 based on HbA1c (%), eGFR (mL/min/1.73 m²), serum creatinine (µmol/L), and urinary ACR (µg/mg).

The second patient, a 34-year-old male, diagnosed with diabetes at age 23 years was referred for MODY evaluation 10 years after diabetes onset while on multiple daily insulin injections with insulin glargine and insulin glulisine with total daily dose of 27 units/day or 0.5 unit/kg. Notably, he presented with multicystic kidneys in utero and had chronic hypomagnesemia and hypokalemia during childhood. A few foci of parenchymal calcification were observed in the kidneys but hydronephrosis was not detected. He had stable renal function for several years after diabetes onset but recently, his eGFR began to decline rapidly at -4.67 mL/min/1.73 m² per year, accompanied by suboptimal glycemic control with HbA1c between 7.0% and 8.5% (53 to 69.4 mmol/mol) albeit absence of albuminuria (uACR <30 μg/mg), suggesting nonalbuminuric CKD (Figure 2). In addition, recent ophthalmological examination ruled out diabetic retinopathy, suggesting non-diabetes-related CKD.

Figure 2.

Longitudinal renal trajectory of Patient 2 based on HbA1c (%), eGFR (mL/min/1.73 m²), and urinary ACR (µg/mg).

The third patient, a 29-year-old male, presented with congenital lateral rectus palsy in his right eye and dysplastic right kidney at 9-year-old which precedes diabetes onset 20 years later. He was subsequently referred for MODY evaluation (particularly HNF1B-MODY) upon diagnosis of diabetes. Renal ultrasound performed prior to genetic diagnosis suggested that his left kidney is normal in size with increased echogenicity and several cysts present. There was no hydronephrosis nor calcification in both kidneys. Upon diagnosis of diabetes with HbA1c of 14.0% (130 mmol/mol) in 2019, he was started on multiple daily insulin injections with insulin glargine and glulisine with total daily dose of 32 units/day or 0.8 unit/kg. He achieved good glycemic control over 7 months upon treatment with HbA1c between 6.3 and 6.6% (45 to 49 mmol/mol) (Figure 3). Based on his renal trajectory, his kidney function is presently well-preserved (eGFR >90 mL/min/1.73 m²) with absence of albuminuria. It would be noteworthy to continually observe his long-term renal outcome. Notably, genital examination excluded structural genital malformations.

Figure 3.

Longitudinal renal trajectory of Patient 3 based on HbA1c (%), serum creatinine (µmol/L), and eGFR (mL/min/1.73 m²).

The fourth patient, a 74-year-old male, was diagnosed with diabetes at age 35 and evaluated for MODY 38 years after diabetes onset. Renal-trajectory over 15 years suggests progressive decline in renal function based on declining eGFR at 1.125 mL/min/1.73 m² per year and presence of prolonged macroalbuminuria (uACR >300 µg/mg). The onset and progression of renal-injury was most probably attributable to long standing diabetes (more than 30 years) and long-term variable HbA1c and off-target glucose control (Figure 4).¹⁴ Abdominal ultrasound showed that both kidneys were normal in size but with increased echogenicity and well-defined cysts in the left kidney. However, no focal mass or hydronephrosis was detected. He is currently treated with dual oral anti-diabetic agents of metformin and a sulphonylurea, along with an angiotensin receptor blocker for the albuminuria.

Figure 4.

Longitudinal renal trajectory of Patient 4 based on HbA1c (%), eGFR (mL/min/1.73 m²), urinary ACR (µg/mg), and serum creatinine (µmol/L).

Taken together, there appears to be 2 patterns of renal deterioration. Patients 1 and 2 showed the atypical features of fairly steep decline in eGFR, despite relatively modest albuminuria. In contrast, patient 4 experienced progressive albuminuria and gradual eGFR decline, which is the more classical pattern expected of diabetic kidney disease (DKD).

We also evaluated the 17-item HNF1B risk score developed by Faguer et al¹⁰ in all 4 patients. Particularly, 2 of the patients had a risk score <8 (cutoff of 8 was reported to be the optimal threshold with a sensitivity of 98.2%, a specificity of 41.1%, and a positive predictive value [PPV] of 19.8%) at the point of initial presentation. Importantly, after the genetic diagnosis has been ascertained by nucleotide-sequencing, subsequent re-evaluation of their clinical features (eg, abdominal imaging, biochemistry tests) led to a re-assignment of higher risk scores. This suggested that the extent of evaluation prompted by clinical indications (eg, with or without abdominal imaging), will affect the performance of such a risk score. This highlights the limitation of the current HNF1B risk score, because of its reliance upon information on intra-abdominal organs structural abnormalities, that is often not routinely or adequately assessed in real-world resource-limited diabetes clinics, unless CKD or other indications such as rapid renal function decline or recurrent urinary tract infections are present or HNF1B-related disease is strongly suspected. In addition, the MODY probability calculators developed by Shields et al¹³ and Ang et al¹² (optimal Youden index-informed cutoff of 17.5%, will yield a sensitivity of 76.0%, specificity of 72.6% and PPV of 37.3%, NPV of 93.4% and accuracy of 73.2%), were also evaluated (Table 1).

Discussion

A nontrivial subpopulation of people with young-onset atypical diabetes has MODY.^11,12 Our experience suggested that a structured clinical-algorithm, supported by candidate-genes massively parallel high-throughput nucleotide sequencing could identify a meaningful proportion (~15%) of individuals with major subtypes of MODY. Notably, among these 40 individuals, 4 (10%) were diagnosed with HNF1B-MODY attributable to HNF1B gene mutations. However, given the rarity of HNF1B-MODY, it is largely undiagnosed in the diabetes clinics. We wish to describe 3 important clinical observations. First, our data suggested that the proposed 17-item HNF1B risk score may not work well in the diabetes clinic because of its heavy reliance on documenting intra-abdominal organ structural abnormalities (eg, CAKUT), which is not part of real-world routine diabetes care, without prior indication for such abdominal imaging. In addition, liver abnormalities and high uric acid levels are nonspecific and electrolytes such as magnesium and even parathyroid hormones are not routinely measured. The MODY probability calculators developed by Shields et al and Ang et al were based on different MODY subtypes and therefore, are not specifically targeted at HNF1B-MODY. Nevertheless, the adoption of poly-genetic risk score¹⁵ may help to identify T1D and T2D among individuals with young-onset diabetes, thereby enriching the remaining subjects for monogenic diabetes gene testing such as HNF1B-MODY. Second, HNF1B-MODY is unique because of its distinctive genetic architecture, that is, HNF1B whole-gene deletion, as part of the chromosome 17q12 syndrome in about 50% of affected individuals.^7,8 This results in a myriad of clinical phenotype, which includes structural kidney anomalies, that compounds the susceptibility to CKD, given the context of on-going long-term diabetes-mediated metabolic injury. Third, the longitudinal renal trajectory of people with HNF1B-MODY has not been well described. Our preliminary data suggested 2 possible patterns: (1) eGFR decline in-tandem with incremental albuminuria, conforming with classical DKD, largely attributable to long-term suboptimal glycemic control and (2) fairly rapid eGFR decline with no or minimal albuminuria, atypical of diabetic nephropathy (DN).¹⁶ Therefore, the latter atypical pattern of renal progression, which is uncommon among Asians (estimated ~5% of our diabetic population),¹⁷ may prompt the request for renal-imaging to search for unexpected diagnosis like HNF1B-MODY associated renal lesions.

Nonetheless, subsequent biochemistry lab tests revealed hypomagnesemia in 3 out of 4 patients and abnormalities of the liver in the form of elevated concentrations of transaminases (alanine aminotransferase and aspartate aminotransferase) in 2 out of 4 patients. Neurodevelopmental or neuropsychiatric disorder which is another common feature (>50% occurrence) related to the 17q12 syndrome was either absent or not formally assessed. The increased risk for neurodevelopmental and neuropsychiatric disorders, such as developmental delay, intellectual disability, autism, and schizophrenia are thought to be attributed by genes other than HNF1B in the 17q12 region, namely LHX1 and ACACA.^18-20 Conversely, the lack of individuals with neurological disease associated with mutations in either of these two candidate genes and the variable severity or absence of neurological disease among 17q12 deletion carriers support a more complex disease model.^7,21 Assessing the extent to which 17q12 genomic disarrangement is associated with neuropsychological disorders will require comprehensive evaluation in all affected patients.

Taken together, the recognition and diagnosis of HNF1B-associated nephropathy is even more challenging when CKD accompanied by diabetes is frequently misdiagnosed as common garden DN and CKD in combination with renal cysts is easily misdiagnosed as autosomal dominant tubulointerstitial disease (ADTKD).²² This is further complicated when the phenotypes associated with HNF1B mutations partly overlap with mutations in other genes (eg, REN, UMOD, PKD1, PKD2 and MUC1).²³ Furthermore, HNF1B has recently been identified as a modifier gene in autosomal dominant polycystic kidney disease (ADPKD)—caused by mutations in the PKD1 or PKD2 genes.²⁴ Therefore, the use of tolvaptan may be explored in HNF1B-MODY patients to slow down the formation of cysts and protect kidney function.^25,26 Additionally, the therapeutic role of SGLT2 inhibitor (a highly effective treatment for CKD) in HNF1B-associated nephropathy is unclear, given the concern of increased susceptibility to uro-genital sepsis (a known contraindication for SGLT2 inhibitor) in the presence of CAKUT.²⁷ Hence, future studies will be needed to address these important clinical issues.

Conclusion

HNF1B-MODY is often a serious multi-systemic disease, partly because of its unique genetic architecture, for example, chromosomal microdeletion with multiple gene-loss. However, the diagnosis can be easily missed among young-onset diabetes in a resource-limited routine-care clinic. Hence, collaboration with a MODY-evaluation center may fill the clinical care-gap.

Additionally, HNF1B-MODY is uniquely vulnerable to CKD due to concomitant CAKUT and early-onset diabetes. Therefore, the long-term renal trajectories of HNF1B-MODY will require further studies by dedicated registries and international consortium.

In the era of precision medicine, the accurate molecular diagnosis of HNF1B-MODY is a feasible and realizable goal, to provide personalized care for affected patients and their families.

Footnotes

Acknowledgements

We would like to thank the patients for their participation and care-providers for their referrals without which this study would not have been possible.

Authors Note

Molly May Ping Eng, MB, BCh, BAO, MRCS, FRCS, is now affiliated to Department of Urology, Khoo Teck Puat Hospital, Singapore.

Allen Yan Lun Liu, MBChB, MRCP, is now affiliated to Department of Renal Medicine, Khoo Teck Puat Hospital, Singapore.

Declaration of Conflicting Interests

The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Funding

The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This research is funded by Alexandra Health Pte Ltd Science—Translational and Applied Research grants STAR17201, STAR18107, and STAR19111.

Ethics Approval

Ethical approval to report this case series was obtained from the Singapore National Healthcare Group Domain Specific Review Board (DSRB Study Reference No. 2011/00232, 2012/01131 and 2017/00276).

Informed Consent

Written informed consent was obtained from the patients for their anonymized information to be published in this article.

ORCID iDs

Bing Xing Goh

Wann Jia Loh

Su Chi Lim

References

Horikawa

Iwasaki

Hara

, et al. Mutation in hepatocyte nuclear factor-1 beta gene (TCF2) associated with MODY. Nat Genet. 1997;17(4):384-385.

Nkonge

TN.

The epidemiology, molecular pathogenesis, diagnosis, and treatment of maturity-onset diabetes of the young (MODY). Clin Diabetes Endocrinol. 2020;6:20. doi:10.1186/s40842-020-00112-5.

Juszczak

Owen

KR.

Identifying subtypes of monogenic diabetes. Diabetes Manage. 2014;4:13.

Roehlen

Hilger

Stock

, et al. 17q12 deletion syndrome as a rare cause for diabetes mellitus type MODY5. J Clin Endocrinol Metab. 2018;103(10):3601-3610.

Ferrè

Igarashi

New insights into the role of HNF-1B in kidney (patho)physiology. Pediatr Nephrol. 2019;34(8):1325-1335.

Clissold

Hamilton

Hattersley

Ellard

Bingham

HNF1B-associated renal and extra-renal disease: an expanding clinical spectrum. Nat Rev Nephrol. 2015;11(2):102-112.

Laffargue

Bourthoumieu

Llanas

Towards a new point of view on the phenotype of patients with a 17q12 microdeletion syndrome. Arch Dis Child. 2015;100(3):259-264.

Mitchel

Moreno-De-Luca

Myers

, et al. 17q12 recurrent deletion syndrome. In: Adam

Ardinger

Pagon

, et al, eds. GeneReviews® [Internet]. Seattle: University of Washington, Seattle; 1993-2021. https://www.ncbi.nlm.nih.gov/books/NBK401562/. Published October 15, 2020. Accessed December 9, 2021.

Dubois-Laforgue

Cornu

Saint-Martin

, et al. Diabetes, associated clinical spectrum, long-term prognosis, and genotype/phenotype correlations in 201 adult patients with hepatocyte nuclear factor 1B (HNF1B) molecular defects. Diabetes Care. 2017;40:1436-1443.

10.

Faguer

Chassaing

Bandin

, et al. The HNF1B score is a simple tool to select patients for HNF1B gene analysis. Kidney Int. 2014;86(5):1007-1015.

11.

Ang

Lim

Tan

CSH

, et al. A preliminary study to evaluate the strategy of combining clinical criteria and next generation sequencing (NGS) for the identification of monogenic diabetes among multi-ethnic Asians. Diabetes Res Clin Pract. 2016;119:13-22.

12.

Ang

Tan

CSH

Chan

LWT

, et al. Clinical experience from a regional monogenic diabetes referral centre in Singapore. Diabetes Res Clin Pract. 2020;168:108390.

13.

Shields

McDonald

Ellard

Campbell

Hyde

Hattersley

AT.

The development and validation of a clinical prediction model to determine the probability of MODY in patients with young-onset diabetes. Diabetologia. 2012;55(5):1265-1272.

14.

Low

SKM

Lim

Yeoh

, et al. Effect of long-term glycemic variability on estimated glomerular filtration rate decline among patients with type 2 diabetes mellitus: insights from the diabetic nephropathy cohort in Singapore. J Diabetes. 2017;9(10):908-919.

15.

Udler

McCarthy

Florez

Mahajan

Genetic risk scores for diabetes diagnosis and precision medicine. Endocrine Reviews. 2019;40:1500-1520.

16.

Adler

Stevens

Manley

, et al. UKPDS GROUP. Development and progression of nephropathy in Type 2 diabetes: The United Kingdom Prospective Diabetes Study (UKPDS 64). Kidney Int. 2003;63:225-232.

17.

Low

SKM

Sum

Yeoh

, et al. Prevalence of chronic kidney disease in adults with type 2 diabetes mellitus. Ann Acad Med Singap. 2015;44(5):164-171.

18.

Bedont

LeGates

Slat

, et al. Lhx1 controls terminal differentiation and circadian function of the suprachiasmatic nucleus. Cell Rep. 2014;7(3):609-622.

19.

Zhao

Kwan

Mailloux

, et al. LIM-homeodomain proteins Lhx1 and Lhx5, and their cofactor Ldb1, control Purkinje cell differentiation in the developing cerebellum. Proc Natl Acad Sci USA. 2007;104(32):13182-13186.

20.

Girirajan

Dennis

Baker

, et al. Refinement and discovery of new hotspots of copy-number variation associated with autism spectrum disorder. Am J Hum Genet. 2013;92(2):221-237.

21.

Clissold

Ashfield

Burrage

, et al. Genome-wide methylomic analysis in individuals with HNF1B intragenic mutation and 17q12 microdeletion. Clin Epigenet. 2018;10:97.

22.

Verhave

Bech

Wetzels

JFM

Nijenhuis

Hepatocyte nuclear factor 1B-associated kidney disease: more than renal cysts and diabetes. J Am Soc Nephrol. 2016;27:345-353.

23.

Groopman

Povysil

Goldstein

Gharavi

AG.

Rare genetic causes of complex kidney and urological diseases. Nat Rev Nephrol. 2020;16(11):641-656.

24.

Shao

Chan

Igarashi

Role of transcription factor hepatocyte nuclear factor-1B in polycystic kidney disease. Cell Signal. 2020;71:109568. doi:10.1016/j.cellsig.2020.109568.

25.

Torres

Chapman

Devuyst

, et al. Tolvaptan in patients with autosomal dominant polycystic kidney disease. N Engl J Med. 2012;367(25):2407-2418.

26.

Gansevoort

van Gastel

MDA

Chapman

, et al. Plasma copeptin levels predict disease progression and tolvaptan efficacy in autosomal dominant polycystic kidney disease. Kidney Int. 2019;96(1):159-169.

27.

Tuttle

Brosius

III Cavender

, et al. SGLT2 Inhibition for CKD and cardiovascular disease in type 2 diabetes: report of a scientific workshop sponsored by the National Kidney Foundation. Am J Kidney Dis. 2020;77(1):94-109. doi:10.1053/j.ajkd.2020.08.003.