In order to obtain high level of β-glucosidase and filter paper activity production, the screening of strain combinations and the characteristic of enzymes from co-culture were investigated. The results showed that both the β-glucosidase (0.347 U/ml) and FPA (3.21 U/ml) reached the highest level via co-culture of T.reesei and T.koningii with the corn straw of alkali pretreatment as fermentation substrate. After 72 h of fermentation, both two enzyme activities reached the highest level. And the optimal temperature and pH value were 55°C and 5.5, respectively. These two enzyme stability decreased rapidly when the temperatures exceeded 90°C. The conversion efficiency reached 0.652 g reducing sugar per g pretreated corn straw using the enzymes from these two fungi, which was 1.49-fold and 1.64-fold compared with those from T.reesei (0.439 g/g) and T.koningii (0.398 g/g). The present work demonstrated an effective way to develop an efficient system for the bioconversion of agricultural waste straws to sugar.
SinghA.N. S., BishnoiN.R.Enzymatic hydrolysis of chemically pretreated rice straw by two indigenous fungal strains: a comparative study. J. Sci. Ind. Res., 69 (2010) 232–237.
2.
BjerreA. B., OlesenA.B.Pretreatment of wheat straw using combined wet oxidation and alkaline hydrolysis resulting in convertible cellulose and hemicellulose. Biotechnology and Bioengineering, 49(5) (1996) 568–577.
3.
CarrilloF.L. M., ColomX.Effect of alkali pretreatment on cellulase hydrolysis of wheat straw: Kinetic study. Process Biochemistry, 40(10) (2005) 3360–3364.
4.
ChenM., ZhaoJ.Comparison of four different chemical pretreatments of corn stover for enhancing enzymatic digestibility. Biomass and Bioenergy, 33(10) (2009) 1381–1385.
5.
ChengS.J., YangP.Z.Expression of multi-functional cellulase gene mfc in Coprinus cinereus under control of different basidiomycete promoters. Bioresource Technology, 100(19) (2009) 4475–4480.
6.
DuffS.J.B., M. W. Bioconversion of forest products industry waste cellulosics to fuel ethanol: a review. Biores. Technol., 55 (1996) 1–33.
7.
GhoseT.K.Measurement of cellulase activities. Pure and Applied Chemistry, 59 (1987) 257–268.
8.
GLM.Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry, 31 (1959) 426–428
9.
GonzálezG. L. p.-S. n. J., CaminalG., SolaC.Dilute acid hydrolysis of wheat straw hemicellulose at moderate temperature: a simplified kinetic model. Biotechnol. Bioeng. Symp., 28 (1986) 288–293.
10.
GurpreetS.D., HarinderS.O., SurinderK.Value-addition of agricultural wastes for augmented cellulase and xylanase production through solid-state tray fermentation employing mixed-culture of fungi, Industrial Crops and Products, 34 (2011) 1160–1167.
11.
Ikram-ul-HaqJ.M.M., KhanT.S.An innovative approach for hyperproduction of cellulolytic and hemicellulolytic enzymes by consortium of Aspergillus niger MSK-7 and Trichoderma viride MSK-10. Afr. J. Biotechnol., 5(8) (2006) 609–614.
12.
JuhaszT.K.K., SzengyelZ.Production of beta-glucosidase in mixed culture of Aspergillus niger BKMF 1305 and Trichoderma reesei RUT C30. Food Technology And Biotechnology, 41(1) (2003) 49–53
13.
KonoH.K.S., ErataT.Regioselective syntheses of new tri- and tetrasaccharides by transglycosylation of Trichoderma viride beta-glucosidase. Journal of Carbohydrate Chemistry, 19(2) (2000) 127–140.
14.
LiC.L., KnierimB.Comparison of dilute acid and ionic liquid pretreatment of switchgrass:Biomass Recalcitrance, Delignification and Enzymatic Saccharification Bioresource Technology, 101(13) (2010) 4900–4906.
15.
LinY.C., MinGuiguang Ling. A method of purification, identification and characterization of beta-glucosidase from Trichoderma koningii AS3.2774. Journal of Microbiological Methods, 83(1) (2010) 74–81.
16.
MassadehM.I., Wan-YusoffW.M., OmarO., and KaderJ.Synergism of cellulase enzymes in mixed culture solid substrate fermentation. Biotechnol. Lett., 23 (2001) 1771–1774.
17.
McIntoshS., and VancovT.Enhanced enzyme saccharification of Sorghum bicolor straw using dilute alkali pretreatment. Bioresour. Technol., 101(17) (2010) 6718–6727.
18.
Monchan MaketonJ.A., and SiriraweekuChatchai. Greenhouse evaluation of Bacillus subtilis AP-01 and Trichoderma harzianum AP-001 in controlling tobacco diseases. Braz. J. Microbiol., 39(2) (2008) 296–300
19.
PanagiotopoulosB., Claassende Vrije, Koukios. Dilute-acid pretreatment of barley straw for biological hydrogen production usingCaldicellulosiruptor saccharolyticus. International Journal of Hydrogen Energy, 37(16) (2012) 11727–11734.
20.
PribowoA.A., ValdeirSaddler, JackN.The adsorption and enzyme activity profiles of specific Trichoderma reesei cellulase/xylanase components when hydrolyzing steam pretreated corn stover. Enzyme and Microbial Technology, 50(3) (2012) 195–203.
21.
SchafnerD.W.T., R T. Cellulase production in continuous culture by Trichoderma reesei on xylose-based media. Biotechnology and Bioengineering, 39(8) (1992) 50–56.
22.
SunY., and ChengJ.Y.Hydrolysis of lignocellulosic materials for ethanol production: a review. Bioresource Technology, 83(1) (2002) 1–11.
23.
SutcliffeR.S.J.The role of lignin in adsorption of cellulases during enzymic treatment of lignocellulosic material. Biotechnol. Bioeng. Symp., 17 (1986) 749–762.
24.
WeimerP.J., and ZeikusJ.G.Fermentation of cellulose and cellobiose by Clostridium thermocellum in absence and presence of methano bacteriumThermoauto trophicum. Applied and Environmental Microbiology, 33(2) (1977) 289–297.
25.
WenZ.Y., LiaoW.Production of cellulase/beta-glucosidase by the mixed fungi culture Trichoderma reesei and Aspergillus phoenicis on dairy manure. Process Biochemistry, 40(9) (2005) 3087–3094.
