Abstract
The morphological architecture and enzymatic hydrolysis of Brassica campestris straw after pretreatment with alkali and then with laccase was investigated. The results show that the diameters and depths of holes on the surface using the alkali and laccase pretreatment are larger than those observed with alkali pretreatment alone. At the same time, the crystallinity indices after alkali and laccase pretreatment are 1.1% higher compared with those observed after alkali pretreatment alone. The components were investigated, and saccharification analysis was carried out. The results showed that the contents of glucose (121 ± 1.1 g/L), xylose (55.9 ± 1.4 g/L), and total reducing sugar (195.3 ± 8.2 g/L) with the alkali and laccase treatment are 38, 193, and 69% higher than with the alkali pretreatment alone. Correspondingly, saccharification reaches 48.8 and 28.9% respectively with the alkali and laccase pretreatment and with the alkali treatment alone.
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