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Abstract

Primary systemic immunoglobulin light chain (AL) amyloidosis is caused by a plasma cell clone of, usually low, malignant potential that expresses CD38 molecules on their surface. Treatment of AL amyloidosis is based on the elimination of the plasma cell clone. The combination of cyclophosphamide–bortezomib–dexamethasone (CyBorD) is the most widely used and is considered a standard of care; however, complete hematologic response rates and organ response rates remain unsatisfactory. Daratumumab, an anti-CD38 monoclonal antibody, has demonstrated encouraging results, with rapid and deep responses, in patients with relapsed or refractory AL amyloidosis as monotherapy with a favorable toxicity profile. The large phase-III, randomized, ANDROMEDA study evaluated the addition of daratumumab to CyBorD in previously untreated patients with AL amyloidosis and demonstrated that addition of daratumumab can substantially improve hematologic complete response rates, survival free from major organ deterioration or hematologic progression, and organ responses. In this review, we discuss the role of daratumumab in the treatment of AL amyloidosis, its mechanism of action, and the results of ANDROMEDA study that led to the first approved therapy for AL amyloidosis.

Keywords

daratumumab light chain amyloidosis monoclonal antibody

Introduction

Primary systemic light chain (AL) amyloidosis is a plasma cell dyscrasias characterized by extracellular tissue deposition of insoluble amyloid fibrils, which are generated by misfolded free light chains (FLCs). A small, usually low-grade, lymphoproliferative clone in the bone marrow produces the toxic monoclonal immunoglobulins that can affect almost every organ system, but most frequently the heart, kidneys, liver, nerves, and soft tissues. The incidence of AL amyloidosis ranges approximately from 10 to 15 cases per million persons per year, and the prevalence of the disease has been increased over the last decade from 15 to 40 cases per million people, with an annual rate of 12%,¹ probably due to improved recognition and increased suspicion of the disease. The overall survival (OS) has improved over the past two decades, after the introduction of novel immunotherapeutic anti-plasma cell therapies, with 2-year OS increasing from 42% to 60% and 4-year OS from 31% to 54%.² However, the disease is still incurable and the prognosis is highly dependent on the cardiac involvement and the time of diagnosis.³ Given also that the survival and organ improvement are intrinsically related to hematologic response,⁴ the introduction of more efficacious combinations is highly relevant. Improved outcomes are closely related to deep and rapid hematologic responses. Until recently, there was no approved therapies for AL amyloidosis by either US Food and Drug Administration (FDA) or European Medicines Agency (EMA). Based on the results of the ANDROMEDA study, on 15 January 2021, FDA approved the use of subcutaneous (SC) daratumumab in combination with cyclophosphamide–bortezomib–dexamethasone (CyBorD) for the treatment of newly diagnosed AL amyloidosis patients with Mayo stages I to IIIA (https://www.fda.gov/drugs/drug-approvals-and-databases/fda-grants-accelerated-approval-darzalex-faspro-newly-diagnosed-light-chain-amyloidosis). Later, in June 2021, EMA also approved the combination for this indication.

The current review will provide data on the combination of daratumumab with CyBorD as the new standard of care in patients with AL amyloidosis.

Current treatment of AL amyloidosis

The target of therapy in AL amyloidosis is to eradicate, as fast as possible, the lymphoproliferative clone that produces the precursor protein of amyloid fibrils. The achievement of at least very good partial response (VGPR) has been associated with improved OS and improved organ function.^5,6 The mainstay of therapy is regimens that have been used in multiple myeloma (MM), adjusted for schedule and dose. The use of alkylating agents has held a central place in the therapy of AL amyloidosis for many decades. Melphalan in combination with prednisone or dexamethasone showed hematologic responses in up to 76% of patients non-eligible for autologous stem cell transplantation (ASCT), with minimal toxicity (mainly myelotoxicity) but with low complete response (CR) rate of 31%.⁷ High-dose melphalan (HDM) followed by ASCT is an effective strategy with high rates of CRs, organ responses, and improved OS.⁸ However, treatment-related mortality (TRM) is higher among patients with AL amyloidosis; thus, HDM/ASCT is used only in strictly selected subjects and only in experienced centers.⁹ Exclusion criteria for ASCT include age, advanced cardiac involvement based on cardiac biomarkers (NT-proBNP and troponin), hypotension, very low serum albumin levels, and multi-organ involvement.

Following advances in MM, proteasome inhibitors (PIs) have been successfully incorporated in the management of AL amyloidosis. Plasma cell clones show intrinsic susceptibility to PIs, probably related to the extensive production of amyloidogenic FLCs, which is also associated with increased proteotoxic stress, altered organelle homeostasis, and reduced autophagy.¹⁰ Bortezomib-based combinations are very effective and well tolerated. Today bortezomib is administered subcutaneously and in a weekly schedule with initial dose of 1.3 mg/m² (if not contraindicated). Bortezomib with dexamethasone induce deep and rapid hematologic responses¹¹ and organ responses.¹² Bortezomib has been successfully combined with alkylating agents for the treatment of AL amyloidosis. In a randomized phase 3 trial, melphalan and dexamethasone (MDex) were combined with bortezomib resulting in 79% of patients to achieve hematological responses at 3 months, including 55% of at least VGPR.¹³ Importantly, this combination resulted also in a significant improvement of the OS compared with MDex. The addition of cyclophosphamide to bortezomib and dexamethasone (CyBorD) has resulted in the most widely used combination for newly diagnosed AL amyloidosis patients. CyBorD has been evaluated in several small studies and large retrospective cohorts with overall hematological response rate reaching 60%, with 43% achieving at least a VGPR, 17% achieving cardiac response, and 25% renal responses.¹⁴ Despite these promising findings, advanced cardiac disease (Mayo stage IIIB high-risk patients with NT-proBNP levels >8500 pg/ml) shows less favorable results with overall response rate (ORR) of 42% and at least VGPR of 23%.¹⁴ In addition, the major adverse events of bortezomib are neurotoxicity and hypotension, and many AL patients have disease-related neuropathy or orthostatic hypotension. Thus, treatment should always be tailored to individual patients’ characteristics. Moreover, there are data supporting lower efficacy of bortezomib-based regimens for patients harboring translocation (11;14),^15,16 which is found in up to 50% of patients, indicating that novel therapeutic combinations are needed.

The introduction of daratumumab

Daratumumab is a human IgGκ1 monoclonal antibody (MoAb) that binds with high affinity to CD38, a surface antigen of both normal and clonal plasma cells. According to studies using flow cytometry or next generation sequencing (NGS), CD38 is ubiquitously expressed on clonal plasma cells in both MM and AL amyloidosis,^17,18 representing an attractive target for immunotherapy. CD38 is a 45-kD type II transmembrane molecule that functions as a transmembrane signaling receptor. Via interacting with its ligand, CD31, it plays an important role in cell survival, adhesion, and migration.¹⁹ Moreover, CD38 acts as an ectoenzyme by converting nicotinamide adenine dinucleotide to cyclic ADP-ribose (adenosine diphosphate ribose) which regulates calcium release and lymphocyte proliferation.²⁰ CD38 is also present, to a lesser extent, on immune effector cells, including regulatory B cells, regulatory T cells, and natural killer (NK) cells.^21
–23 By contrast to MM, high CD38 expression in amyloid plasma cell clone may be correlated with adverse survival, partly because these patients show higher serum NT-proBNP levels and cardiac involvement (p = 0.04).¹⁸

It has been shown that daratumumab induces tumor cell death via a broad-spectrum activity, including direct cellular apoptosis, antibody-dependent cellular cytotoxicity (ADCC), antibody-dependent cellular phagocytosis (ADCP), and complement-dependent cytotoxicity (CDC). Direct apoptosis of CD38+ plasma cells is prompted by Fcγ receptor (FcγR)–mediated cross-linking.²⁴ Both inhibitory and activating FcγRs can result in programmed cell death (PCD) of tumor cells. Loss of the mitochondrial membrane potential, loss of membrane integrity, phosphatidylserine translocation, and morphological changes causing clustering of cells have demonstrated to correlate with cross-linking PCD activation.²⁴ This mechanism of action can enhance efficacy of daratumumab when NK cells or complement are exhausted or when patients are immunosuppressed. Daratumumab interaction with CD38 induces lysis of malignant plasma cells by ADCC and CDC. ADCC capacity is mediated by the activation of NK cells with enhanced degranulation of perforin and granzymes, and other cytotoxic cytokines.²⁵ In addition, the interaction of daratumumab Fc domain with C1q leads to the activation of membrane attack complex (MAC) through the classical pathway and CDC.²⁶ The role of daratumumab in killing tumor cells through ADCP has been explored. The interaction with the Fc receptors (FcRs) on the surface of effector cells (monocytes, macrophages, dendritic) contributes to phagocytosis of malignant plasma cells.²⁷ Besides tumor-targeting mechanisms, daratumumab demonstrates immunomodulatory effects by depletion of CD38+ regulatory T and B cells, and other myeloid-derived suppressor cells (MDSCs).²² Daratumumab exposure may lead to the activation of CD38+ NK cells, but their population is eliminated.²⁸ The reduction in NK cell population leads to expansion of CD4+ Th1 cells and CD8+ effector T cells, which enhances adaptive immune response toward malignant plasma cells.²² Modulation of enzymatic activity has been also observed. Daratumumab inhibits CD38 ectoenzyme function, by decreasing the release of adenosine, an immunosuppressor that eliminates the activity of immune effector cells.²⁵

Daratumumab monotherapy had demonstrated efficacy in pretreated patients with AL amyloidosis in few prospective and several retrospective studies. Kauffman et al. published in 2017 results of a retrospective analysis of 25 consecutive patients with relapsed AL amyloidosis who received daratumumab. The overall hematologic response rate was 76%, including CR in 36% and VGPR in 24%, and median time to response was 1 month. Two prospective phase 2 studies in patients with relapsed or refractory AL amyloidosis report that daratumumab monotherapy induced a VGPR or better hematologic response in 47% and 86% of patients, respectively.^29,30 Median time to hematological response was 1 and 4 weeks. Organ function improvement has also been reported in patients who received daratumumab monotherapy, with renal response in up to 67% and cardiac response in up to 55%.^30,31 Moreover, daratumumab in combination with PIs or immunomodulatory drugs (IMiDs) appears to be safe and highly effective in heavily pretreated patients, with results more favorable than in MM patients.³² In patients with advanced cardiac involvement, daratumumab can rapidly achieve complete remission and it is well tolerated with no signal of cardiac or renal toxicity.³³ These results provided the rationale for exploring daratumumab as part of the frontline therapy in combination with the standard of care CyBorD in the randomized phase 3 ANDROMEDA trial (NCT03201965).

Daratumumab in combination with CyBorD

The approval of SC daratumumab (1800 mg in 15 ml) with recombinant human hyaluronidase PH20, based on the results from COLUMBA trial in patients with MM, is of particular importance for AL amyloidosis. Especially for patients with cardiac involvement, the concern about volume overload is relevant. In addition, the SC daratumumab is associated with fewer administration-related reactions (ARRs). In the setting of newly diagnosed AL amyloidosis, ANDROMEDA study demonstrated for the first time that the addition of SC daratumumab in CyBorD can substantially improve hematologic and organ response rate.³⁴

The design of the ANDROMEDA study was straightforward. Briefly, eligible patients had to have confirmed systemic AL amyloidosis (involving at least one organ) and measurable hematologic disease, no prior therapy for plasma cell dyscrasia, no evidence of symptomatic MM, an estimated glomerular filtration rate of at least 20 ml/min/1.73 m², and were excluded if they had stage IIIB disease per the 2004 Mayo staging system (i.e. if they had stage III disease and an NT-proBNP level >8500 ng/l). Eligible patients were randomized to receive either standard CyBorD for six cycles (weekly dosing, up to 24 doses) or standard CyBorD plus daratumumab for six cycles, and then maintenance therapy with monthly administration of daratumumab for up to 24 cycles for the daratumumab–CyBorD group only.^34,35

There was a safety run-in part in which 28 patients were included.³⁴ The primary goal of this safety run-in was to identify any potential toxicity concerns. The most common treatment emergent adverse events were diarrhea (68%), fatigue (54%), and peripheral edema (50%), which were consistent with the previous experience with both SC daratumumab and CyBorD. Importantly, ARRs occurred in just one patient and was mild (grade 1). There were five deaths, three following autologous transplant and two due to amyloidosis organ progression; none was related to daratumumab–CyBorD therapy.

The ORR with daratumumab–CyBorD in this early small cohort was 96% with VGPR or better in 82% and CR in 36% (in 54% if modified criteria were used that did not require normalization of FLC ratio). The vast majority of responders achieved low difference between involved and uninvolved serum free light chain (dFLC) levels and involved free light chain (iFLC) levels (dFLC < 10 mg/l and iFLC < 20 mg/l), which indicates deep hematologic responses. Median time to first response (at least PR) was just 9 days, median time to VGPR was 19 days, and median time to best response (at least CR) was 85 days. However, daratumumab and other MoAbs are detected by serum immunofixation electrophoresis (IFE) rendering the establishment of CR (which requires negative immunofixation) challenging. A daratumumab-specific IFE reflex assay (DIRA) has been developed to distinguish the monoclonal band derived from therapeutic antibodies from the M-protein.³⁶ In addition, evaluation of minimal residual disease (MRD) using NGS or next generation flow (NGF) cytometry can help in the establishment of a very deep response (i.e. to the point of undetectable MRD). Detection of MRD is clinically relevant as patients with undetectable MRD have a very high probability of organ response and a very low probability of hematologic progression;³⁷ however, in ANDROMEDA, the data on MRD are not mature yet. In patients with cardiac involvement, administration of daratumumab is also feasible and well tolerated. This has been shown in patients with relapsed AL but was also seen in the safety run-in of ANDROMEDA. In the safety run-in section of the study, the overall organ response rate was 64% with 54% achieving cardiac response, 83% renal response, and 50% liver response.

The first interim analysis of the ANDROMEDA was presented in 2020 and results were published in 2021.³⁵ The primary end point of the study was overall hematologic CR rate, as assessed by an Independent Review Committee blinded to treatment. Hematologic CR was slightly different than the International Society of Amyloidosis (ISA) criteria, to account for abnormal FLC ratios due to very low uninvolved immunoglobulin levels. Thus, CR was defined as an iFLC level less than the upper limit of normal with negative serum and urine immunofixation without the requirement of normalized FLC ratio. Secondary end points of ANDROMEDA included major organ deterioration–progression-free survival (MOD-PFS, which was a new composite end point that included end-stage cardiac or renal failure, hematologic progression, or death), organ response rate, OS, 6-month hematologic CR rate, at least hematologic VGPR rate, time to and duration of hematologic CR, time to next treatment, and improvement in fatigue.

A total of 388 patients were enrolled (195 in daratumumab–CyBorD and 193 in the control arm). The characteristics of the patients were well balanced and fairly typical of the population of patients with AL amyloidosis. Although patients with stage IIIB were excluded, ~71% of patients had heart involvement, ~77% stage II or III disease, 36% stage IIIA cardiac disease, and 59% kidney involvement.

After a median follow-up of 11.4 months, hematologic CR rate (the primary end point) was 53.3% in the daratumumab group and 18.1%, corresponding to an odds ratio for CR of 5.1 [95% confidence interval (CI) = 3.2–8.2; p < 0.0001].³⁵ At 6-month landmark analysis, CR rate was 49.7% in the daratumumab–CyBorD versus 14% in CyBorD alone group. Importantly, the median time to hematologic CR was 60 days with daratumumab–CyBorD versus 85 days with CyBorD. VGPR or better rate was 78.5% and 49.2%, respectively (p < 0.0001), while a level of iFLC ⩽20 mg/l (70.8% versus 20.2%) or a level of dFLC <10 mg/l (64.1% versus 30.6%) was also more frequently achieved with the addition of daratumumab. Independently of the criteria used, there was a substantial benefit in terms of deep hematologic responses with the addition of daratumumab. In an update that was presented in 2021 ASCO (American Society of Clinical Oncology) and EHA (European Hematology Association) meetings,³⁸ with additional follow-up (median = 20.3 months), the significant improvement in CR rates was confirmed with 59% versus 19% of patients achieving a CR (OR = 5.9, 95% CI = 3.7–9.4, p < 0.0001) and at least VGPR in 79% versus 50% for daratumumab–CyBorD versus CyBorD, respectively.

The hematologic CR rate was consistent in prespecified subgroups such as patients with more advanced cardiac disease, different age groups, different body weight, and also in those harboring, t(11;14).^35,38 Almost 50% of patients with AL amyloidosis harbor translocation (11;14), and the presence of this abnormality has been associated with inferior response to bortezomib-based therapies (VGPR or better: 52% versus 77%, p = 0.004) and with shorter survival (median OS = 15 versus 27 months, p = 0.05).^15,16 The addition of daratumumab to the standard CyBorD could be even more beneficial for this subgroup of patients with survival disadvantage. It has been reported that in patients treated with daratumumab combinations, the presence of t(11;14) was associated with better hematologic event-free survival (hemEFS).³⁹ Importantly, the addition of daratumumab to CyBorD was able to overcome the adverse impact of t(11;14), and the complete hematologic response rate was similar for patients with and without t(11;14) (55% versus 52% for those without); however, the CR rate was only 13% for those treated with CyBorD (versus 25% for those without the translocation).³⁵

Improvement of organ function, as reflected by organ response rates, is also a major goal of any treatment in AL amyloidosis. A strong consensus in AL amyloidosis is that the majority of patients obtaining a hematologic CR will also obtain a cardiac response and this can radically change patients’ prognosis and survival.

In ANDROMEDA study, among those evaluable for cardiac response, the 6-month cardiac response rate was 41.5% in the daratumumab group and 22.2% in the control group (nominal p = 0.0029). These organ response rates improved further at the 12-month landmark (57% versus 28%). Given that this regimen induces high rates of CR and VGPR, it could significantly improve outcome for stage III AL patients. Patients with Mayo stage IIIB were excluded from ANDROMEDA; however, there is an ongoing trial (NCT04131309) evaluating daratumumab monotherapy in this ultra-high-risk population.

The kidney is the second most frequently affected organ in AL amyloidosis, and proteinuria is the most relevant clinical biomarker for renal survival. MoAbs are safe for patients with nephrotic syndrome and impaired renal function,⁴⁰ while bortezomib in combination with cyclophosphamide and dexamethasone has a protective effect in advanced renal failure due to plasma cell dyscrasias.⁴¹ However, in a series of 168 patients with advanced AL amyloidosis treated with daratumumab in combination with dexamethasone with or without bortezomib, heavy nephrotic-range albuminuria was a significantly adverse factor for hemEFS for both cohorts;³⁹ such patients often have stage III amyloidotic renal disease.⁶ The impaired glomerular barrier in patients with severe non-selective proteinuria leads to excessive urinary losses of MoAbs⁴² and might affect the plasma concentration of therapeutic regimen. There were only few patients with stage III renal disease, and in this subgroup the CR rates were lower but with a wide CI, so that no definite conclusion can be made. However, among those evaluable for renal response (daratumumab group, n = 117; control group, n = 113), the 6-month renal response rates were 53.0% and 23.9%, respectively (nominal p < 0.0001), with 6-month renal progression occurring in 2.6% and 7.1%, respectively, while in the updated 12-month landmark renal response rate remained significantly different (57% versus 27%).

One of the secondary end points in ANDROMEDA was MOD-PFS. This composite end point includes end-stage organ dysfunction (cardiac or renal failure), hematologic progression, or death, whichever occurs first. The MOD-PFS was significantly improved in the daratumumab–CyBorD arm compared with CyBorD alone (hazard ratio = 0.58, 95% CI = 0.36–0.93, p = 0.0211) in the first interim analysis.³⁵ These events included hematologic progression in 8 patients (4.1%) in the daratumumab group versus 25 patients (13.0%) in the control group, major organ deterioration events were 1 (0.5%) and 7 (3.6%), while deaths included 25 (12.8%) versus 21 (10.9%), respectively (deaths occurring after hematologic progression were not counted as events). In addition, significantly more patients required initiation of a new line of therapy in the CyBorD alone group [45 (23%) versus 13 (7%)] compared with daratumumab–CyBorD group.

The median time to next treatment was not reached in the daratumumab group and was 10.4 months in the CyBorD group. In the initial analysis, 19 patients (9.8%) in the daratumumab–CyBorD and 79 patients (42.0%) in the CyBorD arm required and received subsequent therapy, but 48/79 (60.8%) patients in the CyBorD arm received daratumumab-based therapy. Regarding ASCT, 13 patients (6.7%) after daratumumab–CyBorD arm and 20 patients (10.6%) after CyBorD received HDM.

The OS data are not mature yet. In the initial analysis, there was no difference (56 deaths had occurred, 27 in the daratumumab arm and 29 in the control arm);³⁵ at the most recent update, 31 deaths have occurred in the daratumumab–CyBorD arm and 40 deaths in CyBorD arm.³⁸ However, significantly longer follow-up will be required to reveal differences in OS between the two arms.

Safety is a major concern for any regimen used for patients with AL amyloidosis, since most are quite frail with multi-organ dysfunction. In ANDROMEDA, the most common grade 3 or 4 adverse events were lymphopenia; pneumonia (7.8% and 4.3%; it was also the most common in both treatment groups), with grade 3 or 4 infections being 16.6% versus 10.1%; cardiac failure (6.2% and 4.8%); diarrhea (5.7% and 3.7%); syncope (5.2% and 6.4%); neutropenia (5.2% and 2.7%); peripheral edema (3.1% and 5.9%); and hypokalemia (1.6% and 5.3%) for daratumumab–CyBorD and CyBorD, respectively. These were consistent with the previous experience with both CyBorD and with daratumumab. Overall, serious adverse events occurred in 43% of patients in the daratumumab–CyBorD versus 36.2% of patients in CyBorD arm, but these rarely led to treatment discontinuation (in 4.1% versus 4.3%, respectively).

Early deaths (i.e. <60 days from start of therapy) occurred in 13 patients in each group, and overall death was considered as related adverse events in 23 patients (11.9%) in the daratumumab–CyBorD and in 14 patients (7.4%) in CyBorD group; disease progression as a cause of death was reported in 1.0% with daratumumab–CyBorD versus 4.8% with CyBorD. As expected, most adverse events that were considered as leading to death, as well as mortality overall, were mostly observed in patients with advanced cardiac amyloidosis and all patients who died due to cardiac complications had amyloidotic heart involvement.

Patients in the daratumumab–CyBorD arm continued with daratumumab monotherapy after the initial six cycles, but the reported treatment-related adverse events which, during the initial six cycles, occurred in 38% were reduced to 21% after cycle 6,³⁸ while they were 36% in the six cycles of CyBorD alone.

Beyond small administration volume and short administration time, the use of SC daratumumab was also associated with very low rates of systemic ARRs, which occurred in only 14 (7.3%) patients, all of which were mild (grade 1 or 2) and of which 85.7% at the first administration. Nonetheless, all the patients received appropriate pre-medication at least during the initial administrations. No additional systemic ARRs beyond those reported in the primary analysis. Both daratumumab and bortezomib were given subcutaneously, and local injection-site reactions were not uncommon [in 54 patients (28.0%) versus 45 patients (23.9%)], with 21 patients (10.9%) in the daratumumab group experiencing injection-site reactions related to daratumumab.

The shift of daratumumab in combination with CyBorD in the upfront setting of AL amyloidosis arises new challenges for the treatment of relapsed and/or refractory patients. Clinical trials with newer regimens and individualized options for previously treated AL amyloidosis are needed (Table 1). B-cell maturation antigen (BCMA) is generally overexpressed in the surface of clonal plasma cells, including amyloid clones.⁴³ In MM, antibodies targeting BCMA have shown efficacy in relapsed/refractory setting. Currently a phase 2 study with belantamab mafodotin, an antibody-drug conjugate, is ongoing in patients with relapsed/refractory AL amyloidosis (NCT04617925). Alkylating agents have been in the armamentarium of AL amyloidosis treatment for years. The introduction of melphalan flufenamide (melflufen), a novel peptide-drug conjugate that is metabolized to melphalan inside clonal plasma cells, is also evaluated in a phase 1/2 study (NCT04115956). The frequent presence of t(11;14) with overexpression of anti-apoptotic factor BCL-2 (B-cell lymphoma 2) in AL amyloidosis implicates susceptibility to the BCL-2 inhibitor, venetoclax.^44,45 Pending appropriate prospective trials, this might be a promising option for carefully selected patients. Finally, therapies targeting amyloid deposits represent a novel approach to AL amyloidosis therapy. CAEL-101, a light chain amyloid fibril IgG1 MoAb, demonstrated minimal toxicity and significant organ responses in the analysis of phase 1 and phase 2 studies,^46,47 and a randomized phase 3 trial in patients with advanced cardiac involvement is ongoing (NCT04512235 and NCT04504825).

Table 1.

Ongoing clinical trials with daratumumab and novel agents in patients with AL amyloidosis.

ClinicalTrials.gov number	Phase	Agents	Mechanism of action	Population	Status
NCT04512235	3	CAEL-101 plus SoC	Anti-amyloid	NDAL with Mayo IIIA	Recruiting
NCT04504825	3	CAEL-101 plus SoC	Anti-amyloid	NDAL with Mayo IIIB	Recruiting
NCT04973137	3	Birtamimab (NEOD001) plus SoC	Anti-amyloid	NDAL with Mayo IV	Recruiting
NCT04131309	2	Daratumumab	Anti-CD38	NDAL with Mayo IIIB	Recruiting
NCT04895917	2	DaratumumabPomalidomide	Anti-CD38IMiD	RRAL	Recruiting
NCT04617925	2	Belantamab mafodotin	Anti-BCMA	RRAL	Recruiting
NCT04115956	1/2	Melphalan flufenamide (melfluflen)	Peptide-conjugated alkylator	RRAL	Active, not recruiting
NCT03236792	1/2	IxazomibCyclophosphamideDexamethasone	PIAlkylator	NDAL	Recruiting
NCT04942067	1b/2	APG-2575 plus novel therapeutic agents	BCL-2 inhibitor	RRAL	Recruiting
NCT04754945	1	Isatuximab plus CyBorD	Anti-CD38	High-risk NDAL	Recruiting
NCT03283917	1	DaratumumabIxazomibDexamethasone	Anti-CD38PI	NDAL or RRAL	Recruiting

AL, light chain amyloidosis, BCL-2, B-cell lymphoma 2; BCMA, B-cell maturation antigen; CyBorD, cyclophosphamide–bortezomib–dexamethasone; IMiD, immunomodulatory imide drug; NDAL, newly diagnosed AL patients; PI, proteasome inhibitor; RRAL, relapsed or refractory AL patients; SoC, standard of care therapy.

Cardiac amyloidosis

Based on our previous experience and ANDROMEDA results, the administration of daratumumab–CyBorD is overall safe for cardiac amyloidosis patients. The rapid and deep responses observed even after the first administration can improve the outcome of patients with advanced disease. The use of pre-medication for daratumumab includes oral steroids, antihistamine, and montelukast for 2 days prior to the first administration, and dexamethasone, paracetamol, and antihistamine on the day of administration. The approval of SC daratumumab eliminates both volume overload adverse events and allergic reactions. However, the management of ultra-high-risk AL amyloidosis patients (Mayo IIIB, Mayo IV, or patients with severe symptomatic orthostatic hypotension) remains an unmet need.^3,48 This population is often ill-tolerated of CyBorD due to deterioration of heart failure and hypotension; thus, doses should be risk-tailored. We recommend the administration of SC daratumumab 1800 mg with bortezomib at 0.7 mg/m² plus low-dose dexamethasone (20 mg) weekly, and close monitoring for adverse events (AEs). Pre-hydration for patients with severe hypotension may be considered, but it could also exacerbate peripheral edema or dyspnea. Regarding the role of cyclophosphamide, it is questionable today and most probably will be abandoned in the future. The focus will be shifted toward chemotherapy-free combinations, especially for cardiac amyloidosis patients who are extremely frail and prone to chemotherapy toxicities. The combination of novel anti-clonal immunotherapies with anti-amyloid agents, that could efficiently reduce amyloid load from myocardium, holds promise for better survival.

Conclusion

The result of the first phase 3 study in AL amyloidosis has transformed the therapeutic landscape with the introduction of daratumumab in the frontline. The specific characteristics of the amyloid clone make daratumumab an appropriate and promising option in combination with the standard of care CyBorD. The improved tolerability of SC administration is also a novelty of ANDROMEDA trial and it is extremely meaningful for a subgroup of AL amyloidosis patients with concerns about volume overload. Although results from the study regarding daratumumab maintenance and MRD status are still anticipated, the very rapid and deep reduction in toxic FLCs that is observed with daratumumab will improve patients’ prognosis and organ survival.

Footnotes

Author contributions

F.T. and E.K. performed the literature research and drafted the manuscript. M.A.D. and E.K. offered expert opinion and performed revisions.

Conflict of interest statement

The authors declared the following potential conflicts of interest with respect to the research, authorship, and/or publication of this article: E.K. has received honoraria/personal fees from Amgen, Genesis Pharma, Janssen, Takeda, and Prothena, and research grants from Amgen and Janssen. M.A.D. has received honoraria/personal fees from Amgen, BMS, Celgene, GSK, Janssen, and Takeda. FT declares no conclicts of interest.

Funding

The authors received no financial support for the research, authorship, and/or publication of this article.

ORCID iDs

Meletios A. Dimopoulos

Efstathios Kastritis

References

Quock

Yan

Chang

, et al. Epidemiology of AL amyloidosis: a real-world study using US claims data. Blood Adv 2018; 2: 1046–1053.

Muchtar

Gertz

Kumar

, et al. Improved outcomes for newly diagnosed AL amyloidosis between 2000 and 2014: cracking the glass ceiling of early death. Blood 2017; 129: 2111–2119.

Kumar

Dispenzieri

Lacy

, et al. Revised prognostic staging system for light chain amyloidosis incorporating cardiac biomarkers and serum free light chain measurements. J Clin Oncol 2012; 30: 989–995.

Kaufman

Dispenzieri

Gertz

, et al. Kinetics of organ response and survival following normalization of the serum free light chain ratio in AL amyloidosis. Am J Hematol 2015; 90: 181–186.

Palladini

Dispenzieri

Gertz

, et al. New criteria for response to treatment in immunoglobulin light chain amyloidosis based on free light chain measurement and cardiac biomarkers: impact on survival outcomes. J Clin Oncol 2012; 30: 4541–4549.

Palladini

Hegenbart

Milani

, et al. A staging system for renal outcome and early markers of renal response to chemotherapy in AL amyloidosis. Blood 2014; 124: 2325–2332.

Palladini

Milani

Foli

, et al. Oral melphalan and dexamethasone grants extended survival with minimal toxicity in AL amyloidosis: long-term results of a risk-adapted approach. Haematologica 2014; 99: 743–750.

Cibeira

Sanchorawala

Seldin

, et al. Outcome of AL amyloidosis after high-dose melphalan and autologous stem cell transplantation: long-term results in a series of 421 patients. Blood 2011; 118: 4346–4352.

D’Souza

Dispenzieri

Wirk

, et al. Improved outcomes after autologous hematopoietic cell transplantation for light chain amyloidosis: a Center for International Blood and Marrow Transplant Research Study. J Clin Oncol 2015; 33: 3741–3749.

10.

Oliva

Orfanelli

Resnati

, et al. The amyloidogenic light chain is a stressor that sensitizes plasma cells to proteasome inhibitor toxicity. Blood 2017; 129: 2132–2142.

11.

Reece

Hegenbart

Sanchorawala

, et al. Efficacy and safety of once-weekly and twice-weekly bortezomib in patients with relapsed systemic AL amyloidosis: results of a phase 1/2 study. Blood 2011; 118: 865–873.

12.

Kastritis

Wechalekar

Dimopoulos

, et al. Bortezomib with or without dexamethasone in primary systemic (light chain) amyloidosis. J Clin Oncol 2010; 28: 1031–1037.

13.

Kastritis

Leleu

Arnulf

, et al. A randomized phase III trial of melphalan and dexamethasone (MDex) versus bortezomib, melphalan and dexamethasone (BMDex) for untreated patients with AL amyloidosis. Washington, DC: American Society of Hematology, 2016.

14.

Palladini

Sachchithanantham

Milani

, et al. A European collaborative study of cyclophosphamide, bortezomib, and dexamethasone in upfront treatment of systemic AL amyloidosis. Blood 2015; 126: 612–615.

15.

Muchtar

Dispenzieri

Kumar

, et al. Interphase fluorescence in situ hybridization in untreated AL amyloidosis has an independent prognostic impact by abnormality type and treatment category. Leukemia 2017; 31: 1562–1569.

16.

Bochtler

Hegenbart

Kunz

, et al. Translocation t(11;14) is associated with adverse outcome in patients with newly diagnosed AL amyloidosis when treated with bortezomib-based regimens. J Clin Oncol 2015; 33: 1371–1378.

17.

Matsuda

Gono

Shimojima

, et al. Phenotypic analysis of plasma cells in bone marrow using flow cytometry in AL amyloidosis. Amyloid 2003; 10: 110–116.

18.

Seckinger

Hillengass

Emde

, et al. CD38 as immunotherapeutic target in light chain amyloidosis and multiple myeloma – association with molecular entities, risk, survival, and mechanisms of upfront resistance. Front Immunol 2018; 9: 1676.

19.

Deaglio

Morra

Mallone

, et al. Human CD38 (ADP-ribosyl cyclase) is a counter-receptor of CD31, an Ig superfamily member. J Immunol 1998; 160: 395–402.

20.

Ferrero

Malavasi

. Human CD38, a leukocyte receptor and ectoenzyme, is a member of a novel eukaryotic gene family of nicotinamide adenine dinucleotide+-converting enzymes: extensive structural homology with the genes for murine bone marrow stromal cell antigen 1 and aplysian ADP-ribosyl cyclase. J Immunol 1997; 159: 3858–3865.

21.

Flores-Borja

Bosma

, et al. CD19+CD24hiCD38hi B cells maintain regulatory T cells while limiting TH1 and TH17 differentiation. Sci Transl Med 2013; 5: 173ra123.

22.

Krejcik

Casneuf

Nijhof

, et al. Daratumumab depletes CD38+ immune regulatory cells, promotes T-cell expansion, and skews T-cell repertoire in multiple myeloma. Blood 2016; 128: 384–394.

23.

Zambello

Barilà

Manni

, et al. NK cells and CD38: implication for (immuno)therapy in plasma cell dyscrasias. Cells 2020; 9: 768.

24.

Overdijk

Jansen

Nederend

, et al. The therapeutic CD38 monoclonal antibody daratumumab induces programmed cell death via Fcγ receptor-mediated cross-linking. J Immunol 2016; 197: 807–813.

25.

van

Donk

Richardson

Malavasi

. CD38 antibodies in multiple myeloma: back to the future. Blood 2018; 131: 13–29.

26.

de Weers

Tai

Y-T

van der Veer

, et al. Daratumumab, a novel therapeutic human CD38 monoclonal antibody, induces killing of multiple myeloma and other hematological tumors. J Immunol 2011; 186: 1840–1848.

27.

Overdijk

Verploegen

Bögels

, et al. Antibody-mediated phagocytosis contributes to the anti-tumor activity of the therapeutic antibody daratumumab in lymphoma and multiple myeloma. MAbs 2015; 7: 311–321.

28.

Adams

3rd Stevenaert

Krejcik

, et al. High-parameter mass cytometry evaluation of relapsed/refractory multiple myeloma patients treated with daratumumab demonstrates immune modulation as a novel mechanism of action. Cytometry A 2019; 95: 279–289.

29.

Roussel

Merlini

Chevret

, et al. A prospective phase 2 trial of daratumumab in patients with previously treated systemic light-chain amyloidosis. Blood 2020; 135: 1531–1540.

30.

Sanchorawala

Sarosiek

Schulman

, et al. Safety, tolerability, and response rates of daratumumab in relapsed AL amyloidosis: results of a phase 2 study. Blood 2020; 135: 1541–1547.

31.

Chung

Kaufman

Sidana

, et al. Organ responses with daratumumab therapy in previously treated AL amyloidosis. Blood Adv 2020; 4: 458–466.

32.

Abeykoon

Zanwar

Dispenzieri

, et al. Daratumumab-based therapy in patients with heavily-pretreated AL amyloidosis. Leukemia 2019; 33: 531–536.

33.

Hossein Taghizadeh

Thomas

Franz

, et al. Daratumumab – a safe first-line treatment of cardiac AL amyloidosis in heavily compromised patients. In: The XVIth international symposium on amyloidosis, Kumamoto, Japan, 26–29 March 2018.

34.

Palladini

Kastritis

Maurer

, et al. Daratumumab plus CyBorD for patients with newly diagnosed AL amyloidosis: safety run-in results of ANDROMEDA. Blood 2020; 136: 71–80.

35.

Kastritis

Palladini

Minnema

, et al. Daratumumab-based treatment for immunoglobulin light-chain amyloidosis. N Engl J Med 2021; 385: 46–58.

36.

van

Donk

Otten

El Haddad

, et al. Interference of daratumumab in monitoring multiple myeloma patients using serum immunofixation electrophoresis can be abrogated using the daratumumab IFE reflex assay (DIRA). Clin Chem Lab Med 2016; 54: 1105–1109.

37.

Kastritis

Kostopoulos

Theodorakakou

, et al. Next generation flow cytometry for MRD detection in patients with AL amyloidosis. Amyloid 2021; 28: 19–23.

38.

Kastritis

Sanchorawala

Merlini

, et al. Subcutaneous daratumumab + bortezomib, cyclophosphamide, and dexamethasone (VCd) in patients with newly diagnosed light chain (AL) amyloidosis: updated results from the phase 3 ANDROMEDA study. J Clin Oncol 2021; 39: 8003–8003.

39.

Kimmich

Terzer

Benner

, et al. Daratumumab for systemic AL amyloidosis: prognostic factors and adverse outcome with nephrotic-range albuminuria. Blood 2020; 135: 1517–1530.

40.

Rocchi

Tacchetti

Pantani

, et al. Safety and efficacy of daratumumab in dialysis-dependent renal failure secondary to multiple myeloma. Haematologica 2018; 103: e277–e278.

41.

Chanan-Khan

Kaufman

Mehta

, et al. Activity and safety of bortezomib in multiple myeloma patients with advanced renal failure: a multicenter retrospective study. Blood 2007; 109: 2604–2606.

42.

Jacobs

Langer-Jacobus

Duong

, et al. Detection and quantification of rituximab in the human urine. J Immunol Methods 2017; 451: 118–121.

43.

Bal

Sigler

Chan

, et al. First description of B cell maturation antigen expression in light chain amyloidosis. Washington, DC: American Society of Hematology, 2019.

44.

Yip

Lau

JSM

Lam

. Venetoclax monotherapy induced rapid and sustained response in a frail patient with refractory AL amyloidosis: less is more? Int J Hematol 2020; 112: 234–237.

45.

Leung

Thomé

Dispenzieri

. Venetoclax induced a complete response in a patient with immunoglobulin light chain amyloidosis plateaued on cyclophosphamide, bortezomib and dexamethasone. Haematologica 2018; 103: e135–e137.

46.

Edwards

Gould

Langer

, et al. Interim analysis of the phase 1a/b study of chimeric fibril-reactive monoclonal antibody 11-1F4 in patients with AL amyloidosis. Amyloid 2017; 24(Suppl. 1): 58–59.

47.

Khouri

Anwer

Samaras

, et al. Safety, tolerability and efficacy of Cael-101 in AL amyloidosis patients treated on a phase 2, open-label, dose selection study to evaluate the safety and tolerability of Cael-101 in patients with AL amyloidosis. Blood 2020; 136: 21.

48.

Wechalekar

Schonland

Kastritis

, et al. A European collaborative study of treatment outcomes in 346 patients with cardiac stage III AL amyloidosis. Blood 2013; 121: 3420–3427.

Daratumumab plus CyBorD for patients with newly diagnosed light chain (AL) amyloidosis

Abstract

Keywords

Introduction

Current treatment of AL amyloidosis

The introduction of daratumumab

Daratumumab in combination with CyBorD

Cardiac amyloidosis

Conclusion

Footnotes

Author contributions

Conflict of interest statement

Funding

ORCID iDs

References