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Abstract

Non-alcoholic fatty liver disease (NAFLD) and atherosclerosis (AS) are two major phenotypes of metabolic syndrome that frequently coexist through a “liver–vascular axis” characterized by lipid dysregulation, chronic inflammation, and mitochondrial dysfunction. The liver kinase B1/AMP-activated protein kinase (LKB1/AMPK) signaling pathway, acting as a master regulator of energy and redox balance, has emerged as a central hub in this comorbidity. Activation of this pathway suppresses lipogenesis, enhances fatty acid oxidation, attenuates inflammation, and improves endothelial function, thereby interrupting the vicious metabolic–inflammatory cycle underlying NAFLD and AS. Natural products provide promising multi-target modulators of LKB1/AMPK. Polyphenols (such as curcumin, resveratrol, quercetin), terpenoids (such as nobiletin, betulinic acid), alkaloids (such as berberine), and glycosides (such as ginsenosides, salidroside) restore lipid homeostasis, induce autophagy, regulate oxidative stress, and modulate immune responses via LKB1/AMPK and its crosstalk with sirtuin 1 (SIRT1), peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1α), and mammalian target of rapamycin (mTOR) signaling. Preclinical studies highlight their ability to improve hepatic steatosis, vascular inflammation, and plaque stability, underscoring their cross-organ therapeutic potential. However, pharmacological translation remains limited by poor bioavailability, variable pharmacokinetics, species differences, and inconsistent clinical outcomes. This review systematically summarizes comorbidity mechanisms of NAFLD and AS, regulatory roles of the LKB1/AMPK pathway, therapeutic actions of representative natural products, and the challenges ahead, aiming to guide precision strategies for metabolic syndromes.

Keywords

Phytotherapy LKB1/AMPK pathway liver-vascular axis metabolic-inflammatory network comorbidity

Introduction

Non-alcoholic fatty liver disease (NAFLD) and atherosclerosis (AS) are two core manifestations of the metabolic syndrome that have risen to global prominence. Epidemiological data indicate that NAFLD affects roughly one quarter of the world's population and that AS-related cardiovascular disease remains the leading cause of mortality worldwide.^1–3 Their frequent co-occurrence and reciprocal worsening amplify risks of major adverse cardiovascular events as well as progression to end-stage liver disease, making the NAFLD-AS axis a major clinical and research priority.^4–6 This comorbidity calls for mechanistic frameworks that cross traditional organ boundaries rather than organ-centric approaches.

Mechanistically, NAFLD and AS share a network of interlocking pathologies-lipid dysregulation, chronic inflammation, oxidative stress, and insulin resistance (IR)-that drive reciprocal organ injury.^7–9 Hepatic lipotoxicity promotes atherogenesis via enhanced secretion of very low-density lipoprotein (VLDL), formation of oxidized low-density lipoprotein (ox-LDL), and exosome-mediated suppression of macrophage cholesterol efflux (for example, by miR-30a-3p), while vascular dysfunction and impairment of high-density lipoprotein (HDL) in AS can feed back to aggravate hepatic lipid deposition and fibrogenesis.^10–13 A growing body of imaging, biomarker, and molecular studies therefore supports the concept of a liver-vascular crosstalk in which the liver both signals to and is affected by the atherosclerotic milieu.

Among molecular hubs implicated in this crosstalk, the liver kinase B1/AMP-activated protein kinase (LKB1/AMPK) signaling axis emerges as a central integrator of energy, lipid, and immune homeostasis. LKB1/AMPK functions as a cellular energy sensor that directly phosphorylates targets such as acetyl-CoA carboxylase (ACC)-leading to reduced malonyl-CoA and enhanced carnitine palmitoyltransferase 1 (CPT1)-mediated fatty acid oxidation-while suppressing anabolic programs driven by sterol regulatory element-binding protein 1c (SREBP1c) and 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR). At the same time, it promotes vascular protection by enhancing endothelial nitric oxide synthase (eNOS) activity, inducing autophagy, and inhibiting nuclear factor kappa B (NF-κB).^14–16 These direct AMPK actions are further modulated by secondary but highly relevant axes such as sirtuin 1 (SIRT1), mammalian target of rapamycin (mTOR), and nuclear factor erythroid 2-related factor 2 (Nrf2), which may act downstream of, in parallel with, or independently from AMPK to shape mitochondrial biogenesis, antioxidant defenses, and inflammatory set-points.^17–19 Thus, targeting the LKB1/AMPK hub offers a rational strategy to concurrently ameliorate hepatic steatosis, systemic metabolic dysfunction, and atherogenesis.

Several reviews have previously addressed natural products and the LKB1/AMPK pathway in the context of NAFLD.^20–22 However, these reviews primarily focused on liver-specific disease mechanisms and compound classes, with limited emphasis on vascular pathology or NAFLD–AS comorbidity. In contrast, the present review distinctively emphasizes the bidirectional liver–vascular crosstalk, highlights how natural products acting on LKB1/AMPK may simultaneously mitigate both hepatic and vascular lesions, and critically discusses pharmacological and translational challenges that remain obstacles to clinical application.

Natural products—including polyphenols, terpenoids, alkaloids, and glycosides—have attracted attention because many directly or indirectly activate LKB1/AMPK and simultaneously modulate complementary pathways such as SIRT1, Nrf2, and mTOR, yielding pleiotropic benefits in preclinical NAFLD and AS models (for example, curcumin, ginsenosides, berberine, resveratrol, and epigallocatechin gallate (EGCG)).^23–25 However, a critical translational gap persists: most evidence is preclinical, and progress to the clinic is hampered by poor oral bioavailability, complex pharmacokinetics—including extensive first-pass metabolism and gut microbiota transformation—species-dependent differences, and inconsistent clinical trial outcomes. Addressing these pharmacological and delivery challenges is essential to convert promising mechanisms into effective therapies for patients.^26–28

In this review we (1) synthesize epidemiological and mechanistic evidence linking NAFLD and AS, (2) summarize the biological functions of the LKB1/AMPK pathway that underlie this crosstalk, (3) review natural products that target this hub with emphasis on unique molecular mechanisms and available clinical evidence, and (4) critically discuss pharmacokinetic, safety and translational challenges that must be overcome to enable clinical application. By integrating mechanistic biology with translational realities, we aim to provide a focused roadmap for leveraging LKB1/AMPK-targeting natural products in the management of NAFLD-AS comorbidity.

Method

Literature Search and Selection Criteria for Natural Products

To identify natural products that modulate the LKB1/AMPK signaling pathway in the context of NAFLD, non-alcoholic steatohepatitis (NASH), or AS, a systematic literature search was conducted following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. Five electronic databases—PubMed, Web of Science, Embase, Scopus, and the China National Knowledge Infrastructure—were searched from inception to June 30, 2025. Search terms combined pathway- and disease-related keywords (“LKB1” OR “serine/threonine kinase 11” OR “AMPK” OR “AMP-activated protein kinase”) with disease terms (“non-alcoholic fatty liver disease” OR “NAFLD” OR “non-alcoholic steatohepatitis” OR “NASH” OR “atherosclerosis” OR “AS”) and natural product-related terms (“natural product” OR “phytochemical” OR “herb*” OR specific compound names such as “curcumin”, “berberine”, “resveratrol”). References of retrieved articles and relevant reviews were manually screened for additional studies.

Inclusion and Exclusion Criteria

Eligible studies met the following criteria:

original experimental studies in cell culture (in vitro), animal models (in vivo), or clinical research on plant- or fungi-derived natural products (isolated compounds or defined extracts);

direct experimental evidence of LKB1/AMPK pathway modulation (for example, changes in phosphorylated AMPK at Thr172, phosphorylated LKB1, or downstream targets), or mechanistic validation using AMPK/LKB1 inhibition, knockdown/knockout, or other perturbation experiments; and

outcomes relevant to NAFLD, NASH, or AS (for example, lipid accumulation, inflammation, plaque area, endothelial function, liver enzymes).

Exclusion criteria comprised: review articles, editorials, and conference abstracts without full data; purely computational docking studies without experimental validation; synthetic derivatives without confirmed natural origin; studies lacking direct LKB1/AMPK readouts; non-peer-reviewed reports; and non-English or non-Chinese publications without accessible full text.

Data Extraction and Quality Assessment

Two reviewers independently screened titles, abstracts, and full texts, resolving disagreements by consensus. Extracted data included compound or extract identity, source, study model, dosing regimen, pathway evidence, key outcomes, and pharmacokinetic data where available. Risk of bias was assessed using the Systematic Review Centre for Laboratory animal Experimentation tool for animal studies and the Cochrane risk-of-bias tool for clinical trials. Mechanistic robustness was graded according to pre-specified criteria. The study selection process was documented in a Preferred Reporting Items for Systematic Reviews and Meta-Analyses flow diagram.

Pathological Mechanisms Linking NAFLD and AS

Association Between NAFLD and AS

Epidemiological and Biomarker Evidence

Multiple studies confirm a significant correlation between NAFLD and atherosclerotic cardiovascular risk, independent of confounders such as metabolic syndrome, smoking, and obesity.^1–3 Patients with NAFLD exhibit higher carotid intima-media thickness (CIMT), coronary artery calcium (CAC) scores, and arterial stiffness (brachial-ankle pulse wave velocity; carotid-femoral pulse wave velocity) compared to non-NAFLD individuals.^1–3^,8,13 The severity of NAFLD demonstrates a dose-dependent relationship with AS risk: grade 3 hepatic steatosis correlates with significantly elevated CIMT, while advanced liver fibrosis is linked to increased CAC risk.^4,5 Notably, even in young (<45 years) and non-obese individuals, NAFLD remains independently associated with subclinical AS.^6–8

Beyond imaging-based indices, multiple biomarkers further support the NAFLD–AS link. Inflammatory and metabolic markers such as interleukin-6 (IL-6), C-reactive protein (CRP), Fetuin-A, and pentraxin-3 are elevated in NAFLD and correlate with AS progression, while low family with sequence similarity 19 member A5 levels show negative associations with hepatic steatosis and CIMT.^14–16 Lipid-related markers including high triglyceride/high-density lipoprotein cholesterol (triglyceride [TG]/high-density lipoprotein cholesterol [HDL-C]) ratios, small extracellular vesicle microRNA-30a-3p, and ATP-binding cassette transporter (ABC)-A1/cholesterol 7 alpha-hydroxylase imbalance reflect cholesterol dysregulation in NAFLD–AS crosstalk.^17–19 Molecular pathway markers such as receptor-interacting protein kinase 1, sirtuin 6 (SIRT6), and peroxisome proliferator-activated receptors indicate lipid–inflammation interaction, whereas chemokine (C-C motif) ligand 19 expression and CD4+ T-cell infiltration highlight immune involvement.^17,20,21

Furthermore, clinical risk assessment tools—including non-invasive liver fibrosis scores (fibrosis-4 index; NAFLD fibrosis score) and imaging-based plaque characteristics (non-calcified plaque ratio)—hold predictive value for cardiovascular outcomes in patients with NAFLD.^3,12,22 Together, these findings establish NAFLD as both a structural and molecular predictor of atherosclerotic burden.

Key Mechanisms

The pathological interplay between NAFLD and AS involves both direct effects of the LKB1/AMPK axis and secondary/synergistic pathways (eg, SIRT1; mTOR; Nrf2), which may act independently or downstream of AMPK activation.

Lipid Metabolism Dysregulation: NAFLD disrupts hepatic lipid metabolism, elevating TG, non-HDL-C, and ox-LDL while reducing HDL-C. This promotes VLDL secretion and cholesterol accumulation.^23,24 Liver-derived exosomes (eg, miR-30a-3p) inhibit ABC-A1-mediated cholesterol efflux, driving macrophage foam cell formation and accelerating AS. Direct AMPK-mediated effects include upregulation of ABC-A1/ABC-G1 and scavenger receptor class B type I/lecithin-cholesterol acyltransferase to enhance HDL-dependent reverse cholesterol transport,²⁵ suppression of ox-LDL uptake,²⁶ and promotion of autophagy (FoxO3a–LC3/Unc-51 like autophagy activating kinase 1, ULK1 pathway).²⁷ Secondary mechanisms involve activation of SIRT1 (which independently deacetylates peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) and p65) and inhibition of mammalian target of rapamycin complex 1 (mTORC1), both of which synergistically enhance fatty acid oxidation (FAO), mitochondrial biogenesis, and lipid clearance.

Inflammatory Response: NAFLD activates systemic inflammation through pro-inflammatory factors (tumor necrosis factor-alpha [TNF-α], IL-6, CRP), damaging vascular endothelia and promoting AS plaque formation.^28–31 Direct anti-inflammatory actions of LKB1/AMPK include suppression of NF-κB signaling to reduce IL-6 levels,^25,32 promotion of macrophage M2 polarization,²⁵ and autophagy induction via the AMPK/mTOR pathway.^26,33 Secondary/synergistic effects involve SIRT1-mediated p65 deacetylation, Nrf2 activation (driving antioxidant gene transcription), and further mTORC1 suppression to limit inflammatory gene expression. AMPK activators also reduce HDL inflammatory index and myeloperoxidase/paraoxonase-1 ratios,²⁵ systemically inhibiting inflammatory damage.

Endothelial Dysfunction: NAFLD induces endothelial injury via oxidative stress and lipid peroxidation, exacerbated by IR and elevated free fatty acids.^28,30 Direct AMPK effects include inhibition of protein kinase C (PKC) signaling to reduce ox-LDL-induced endothelial apoptosis³³ and enhancement of eNOS phosphorylation for vasodilation.³² Secondary effects include SIRT1 activation (independently improving endothelial mitochondrial function) and Nrf2-driven reactive oxygen species (ROS) detoxification, which synergize with AMPK-mediated vascular protection.

Oxidative Stress: NAFLD increases hepatic oxidative stress, with malondialdehyde accumulation damaging hepatocytes and endothelia, promoting foam cell formation. Direct AMPK effects include suppression of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity (via thioredoxin; uncoupling protein 2) and clearance of damaged mitochondria through ULK1-mediated autophagy.³³ Secondary contributions arise from Nrf2 activation (which induces antioxidant genes such as heme oxygenase-1, HO-1; NAD(P)H quinone dehydrogenase 1) and SIRT1 signaling, both of which can also be triggered by AMPK to amplify antioxidant defense.

Energy Metabolism Dysregulation: IR, a core mechanism linking NAFLD and AS, drives hepatic lipolysis and free fatty acids release, exacerbating dyslipidemia and endothelial injury. Direct AMPK actions include improving insulin sensitivity, enhancing glucose transporter type 4 (GLUT4) translocation, and suppressing hepatic gluconeogenesis. Secondary and independent pathways include peroxisome proliferator-activated receptor alpha/gamma (PPARα/γ) regulation of lipid oxidation, SIRT6 inhibition of NF-κB inflammation, and angiotensin-converting enzyme 2/angiotensin-(1-7) axis modulation of renin–angiotensin system activity.³⁴ Exosome-mediated organ crosstalk exacerbates metabolic disorders: hepatocyte-derived exosomes impair cholesterol efflux,¹⁹ while AS plaque-derived exosomes worsen hepatic lipid deposition and inflammation.^24,35 Immune cells further disrupt liver–vascular crosstalk via exosomal cytokines or non-coding RNAs.^18,35 Thus, both primary LKB1/AMPK signaling and secondary metabolic–immune pathways jointly underlie NAFLD–AS comorbidity (Figure 1).

Figure 1.

Hepato-Vascular Pathological Interplay in NAFLD-AS Comorbidity. Annotations: LCFA: Long Chain Fatty Acid; CHOL: Cholesterol; WAT: White Adipose Tissue; PAF: Platelet Activating Factor; LM: Leukocyte Migration; ICI: Inflammatory Cell Infiltration;Endo MT: Endothelial Mesenchymal Transition; DM: Dysfunctional Mitochondria; MFI: Mitochondrial Fission Imbalance; IR: Insulin Resistance. Created with BioRender.com.

Biological Functions of the LKB1/AMPK Signaling Pathway

Basic Overview of the LKB1/AMPK Pathway

The LKB1/AMPK signaling pathway is a well-established regulatory hub in metabolic and inflammatory diseases, with extensive evidence from cellular, animal, and human studies linking its dysfunction to NAFLD, cardiometabolic disorders, and cancer.^36,37 AMPK, a heterotrimeric protein kinase (composed of α, β, and γ subunits, forming 12 isoforms), maintains energy homeostasis by sensing intracellular ATP/AMP ratios. LKB1, a key upstream kinase, directly activates AMPK by phosphorylating Thr172 (Thr174 in α2).³⁷ Direct regulation occurs through AMP binding, conformational activation, and prevention of Thr172 dephosphorylation. Secondary regulation involves associated pathways such as SIRT1 (nicotinamide adenine dinucleotide-dependent deacetylase), mTOR (nutrient-sensing kinase), and Nrf2 (antioxidant transcription factor), which can be either AMPK-dependent or independent.

Role of LKB1/AMPK in Metabolic Regulation:

Lipid Metabolism

Direct mechanisms: The LKB1/AMPK axis directly phosphorylates ACC1/ACC2 at Ser79/Ser212,³⁶^38–41 reducing malonyl-CoA levels and thereby blocking fatty acid and cholesterol synthesis via HMG-CoA reductase (HMGR) inhibition,⁴² while relieving malonyl-CoA-mediated suppression of CPT1 to promote mitochondrial long-chain fatty acid β-oxidation.^36,38,43 AMPK also suppresses lipogenic transcription factors (SREBP1c; ChREBP) and downregulates fatty acid synthase (FASN) and Hmgcr expression,³⁹^44–46 and activates CPT1a to enhance fatty acid mitochondrial transport.⁴⁷

Secondary/Synergistic mechanisms: AMPK synergizes with the SIRT1–PGC-1α–PPARα axis (via SIRT1-mediated PGC-1α deacetylation) to upregulate CPT1a and acyl-CoA oxidase 1, promoting mitochondrial and peroxisomal β-oxidation.^48–50 It interacts with mTORC1 inhibition (via tuberous sclerosis complex 2 (TSC2)/regulatory-associated protein of mTOR phosphorylation)^51,52 and enhances nicotinamide adenine dinucleotide metabolism to boost oxidative capacity.⁵⁰ In the hypothalamus, LKB1/AMPK–pro-opiomelanocortin (POMC) neuron activation promotes white adipose tissue (WAT) browning via PR domain containing 16–PPARγ and suppresses hepatic SREBP1c.^53,54 These secondary mechanisms can act independently of AMPK but usually enhance its lipid-lowering effects, contributing to immune regulation (eg, T-cell FAO support⁴⁷ and organ protection (eg, reversing hepatic steatosis and renal fibrosis.^49,55

Inflammatory Response

Direct mechanisms: AMPK inhibits mTORC1 via TSC2 phosphorylation,^36,56 blocks NF-κB and c-Jun N-terminal kinase (JNK) activation, and phosphorylates inhibitor of κB kinase α/β (IKKα/β) to prevent NF-κB nuclear translocation. It also reduces ROS production via PKC–NADPH oxidase inhibition and phosphorylates forkhead box protein O1/3 to induce superoxide dismutase 2 (SOD2)/catalase (CAT) expression.^57,58

Secondary/Synergistic mechanisms: SIRT1-mediated p65 deacetylation and PGC-1α competition with p65 further suppress pro-inflammatory cytokines (TNF-α, IL-1β, IL-6).^54,59,60 LKB1 maintains tricarboxylic acid (TCA) cycle homeostasis to control 2-hydroxyglutarate levels, modulating chromatin accessibility, while AMPK limits inflammatory transcription via poly (ADP-ribose) polymerase 1/histone deacetylase 5 inhibition.^61,62 Nrf2 activation enhances antioxidant gene expression.⁵⁷ Tissue-specific effects include hypothalamic LKB1/AMPK stabilization of inhibitor of κBα to suppress transforming growth factor-beta1 (TGF-β1)–Toll-like receptor 4 (TLR4) signaling⁵³ and intestinal LKB1 maintenance of barrier integrity.⁴⁴ These secondary mechanisms act independently but synergize with AMPK to inhibitNLR family pyrin domain containing 3 (NLRP3) inflammasome activation,⁵⁸ modulate T helper 17/regulatory T (Th17/Treg) balance,^61,62 and reduce lipotoxic inflammation.^58,63

Endothelial Function

Direct mechanisms: AMPK phosphorylates eNOS at Ser1177 to enhance NO production,^41,56 increases eNOS expression, and prevents uncoupling. It also suppresses pro-apoptotic JNK/p38 mitogen-activated protein kinase (MAPK) signaling and inhibits PKC–NADPH oxidase to reduce ROS.^57,59

Secondary/Synergistic mechanisms: SIRT1 activation improves mitochondrial function and supports eNOS activity.⁵⁹ Nrf2-mediated oxidative stress control reduces endothelial inflammation.⁵⁷ LKB1/AMPK also downregulates adhesion molecules (vascular cell adhesion molecule 1, VCAM-1; intercellular adhesion molecule 1, ICAM1; E-selectin) and chemokines (monocyte chemoattractant protein 1, MCP-1; regulated on activation, normal T cell expressed and secreted),⁵⁹ and works with PPARα to maintain endothelial integrity—loss of this axis (eg, high-fat diet) impairs function, whereas PPARα agonists restore it.⁵²

Mitochondrial Function

Direct mechanisms: LKB1/AMPK activates PGC-1α (with nuclear respiratory factor 1) to drive mitochondrial biogenesis, improves oxidative phosphorylation, FAO, and respiratory capacity.^52,63 It phosphorylates ULK1 for mitophagy and mitochondrial fission factor (MFF) for mitochondrial fission, maintaining network dynamics.^36,51,64

Secondary/Synergistic mechanisms: SIRT1 deacetylates PGC-1α to couple mitochondrial biogenesis with quality control,⁵¹ and Nrf2 induction boosts mitochondrial antioxidant defenses.^52,65 During mitochondrial dysfunction, LKB1 restores TCA activity via NADH metabolism and phosphoglycerate dehydrogenase regulation. LKB1/AMPK deficiency in type 2 innate lymphoid cells causes oxidative phosphorylation impairment and ROS accumulation, suppressing IL-5/IL-13 via B-cell lymphoma-extra large (Bcl-xL) inhibition and ROS–nuclear factor of activated T-cells–programmed cell death protein 1 (PD-1) activation, which can be rescued by PD-1 blockade.^61,66

Energy Metabolism

Direct mechanisms: AMPK improves insulin sensitivity by enhancing β-cell insulin synthesis/secretion, upregulating GLUT4 translocation, and suppressing hepatic glucose-6-phosphatase (G6Pase)/phosphoenolpyruvate carboxykinase (PEPCK).^36,38,39,56 It modulates glycolysis (6-phosphofructo-2-kinase activation during ischemia, Warburg effect suppression in tumors) and reduces energy expenditure on protein synthesis.^39,42,56

Secondary/Synergistic mechanisms: peroxisome proliferator-activated receptors, SIRT1, and SIRT6 modulate energy metabolism in cooperation with AMPK.⁵⁰ LKB1 regulates hypothalamic POMC neurons to control appetite and glucose balance,^53,55 and stabilizes PD-1/glucocorticoid-induced TNFR-related protein in Treg cells to modulate immune metabolism.^32,55 The Nrf2 axis enhances antioxidant defenses (SOD2/CAT).^41,54,63 These pathways form a metabolic–inflammation–immune network that can function independently but collectively sustain systemic energy homeostasis.

The integrated multi-organ regulatory roles of LKB1/AMPK signaling in NAFLD and AS are illustrated in Figure 2. The following section explores the therapeutic potential of various natural products that target this multifaceted LKB1/AMPK signaling hub, highlighting their unique mechanistic features and clinical applications in NAFLD and AS.

Figure 2.

Mechanistic Network of LKB1/AMPK-Mediated Multi-Organ Regulation in NAFLD and AS. Annotations: ACC: Acetyl-CoA Carboxylase; SREBP1c: Sterol Regulatory Element-Binding Protein 1c; CPT1A: Carnitine Palmitoyltransferase 1A; PGC-1α: Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-Alpha; PPARα: Peroxisome Proliferator-Activated Receptor Alpha; LCFA: Long Chain Fatty Acid; CHOL: Cholesterol; WAT: White Adipose Tissue; BAT: Brown Adipose Tissue; TSC2: Tuberous Sclerosis complex 2; IKKα/β: Inhibitor of Nuclear Factor Kappa-B Kinase Subunit Alpha/beta; mTORC1: Mechanistic Target of Rapamycin complex 1; NF-κB: Nuclear Factor Kappa B; TNF-α: Tumor Necrosis Factor-Alpha; IL-1β: Interleukin-1 beta; IL-6: Interleukin-6; IL-17: Interleukin-17; VEGF: Vascular Endothelial Growth Factor; eNOS: Endothelial Nitric Oxide Synthase; VCAM-1: Vascular Cell Adhesion Molecule 1; ICAM-1: Intercellular Adhesion Molecule 1; PAF: Platelet Activating Factor; LM: Leukocyte Migration; ICI: Inflammatory Cell Infiltration;Endo MT: Endothelial Mesenchymal Transition; ULK1: Unc-51 Like Autophagy Activating Kinase 1; MFF: Mitochondrial Fission Factor; Bcl-xL: B-Cell Lymphoma-Extra Large; mtDNA: Mitochondrial DNA; DM: Dysfunctional Mitochondria; MFB: Mitochondrial Fission Balance; ILC2 mt: Type 2 Innate Lymphoid Cells Mitochondrial; NPY: Neuropeptide Y; AgRP: Agouti-Related Peptide; POMC: Pro-Opiomelanocortin; CART: Cocaine- and Amphetamine-Regulated Transcript; FoxO1: Forkhead box Protein O1; GLUT4: Glucose Transporter Type 4; G6Pase: Glucose-6-Phosphatase; PEPCK: Phosphoenolpyruvate Carboxykinase. Created with BioRender.com.

Therapeutic Potential of Natural Products Targeting LKB1/AMPK Pathway in NAFLD and AS

LKB1/AMPK pathway serves as a central signaling hub, integrating regulation of lipid metabolism, inflammation, oxidative stress, endothelial function, and mitochondrial homeostasis, as detailed in Section 3. The therapeutic potential of the natural products discussed below primarily arises from their ability to activate this master regulatory axis, thereby invoking its broad spectrum of beneficial downstream effects. Rather than reiterating these common effects (eg, ACC phosphorylation, SREBP1c suppression, eNOS activation), this section will highlight the unique mechanisms, direct molecular targets, clinical translation evidence, and specific bioavailability challenges associated with each compound or herbal extract. The overarching goal is to showcase their multi-targeted and synergistic approaches to disrupting the NAFLD-AS comorbidity network.

Multi-Target Effects of Single Herbal Medicines

Rhizome-Derived Herbs

Turmeric-derived bioactive compounds (tetrahydrocurcumin [THC], fermented turmeric, curcumin) exert their effects primarily by directly activating the LKB1/AMPK axis.^67–71 Unique Mechanisms and Synergies: A unique and direct plaque-stabilizing mechanism of curcumin is the inhibition of macrophage extracellular matrix metalloproteinase inducer (EMMPRIN)/matrix metalloproteinase (MMP) expression, which is independent of its AMPK-mediated anti-inflammatory effects.⁷¹ THC further demonstrates a strong secondary benefit by improving insulin sensitivity through insulin receptor substrate–phosphoinositide 3-kinase–protein kinase B (IRS–PI3K–Akt) signaling.⁶⁷ Clinical Evidence and Challenges: Despite promising preclinical data, clinical evidence is mixed. A randomized controlled trial (RCT) found 1500 mg/day curcumin was not superior to lifestyle intervention alone for NAFLD inflammation,⁷² while meta-analyses suggest benefits for metabolic parameters.⁷³ This discrepancy underscores the critical challenge of its low oral bioavailability, driving the development of novel formulations like BCM-95^®⁷² and nanoparticles.^74–76

Ginseng-derived bioactive compounds (ginsenosides Rg5, Rb2, compound K [CK], Rg1, Rb1, Rg3) mediate their effects through direct activation of the upstream kinase LKB1.^77–82 Unique Mechanisms and Synergies: Rg3 promotes macrophage M2 polarization through PPARγ activation, showcasing an immunomodulatory mechanism that complements its AMPK-mediated effects.^79,81 Rb1 enhances endothelial function via the SIRT1/AMPK axis, providing a vasoprotective synergy.⁸² Clinical Evidence and Challenges: Clinical trials for pure ginsenosides are scarce. A major translational bottleneck is the extremely low bioavailability (<1%) of highly active metabolites like ginsenoside CK,^83–85 necessitating strategies like albumin nanoparticles.⁸⁶

Salvia miltiorrhiza-derived bioactive compounds (salvianolic acid A [SAA], sodium tanshinone IIA sulfonate [STS], Radix Rubiae Paeoniae [RRP], puerarin [PA]) exert multi-target effects via the LKB1/AMPK axis.^87–91 Unique Mechanisms and Synergies: SAA uniquely inhibits ferroptosis, linking AMPK activation to a novel cell death pathway in NAFLD.⁸⁷ STS activates the AMPK/Nrf2/HO-1 pathway, providing a potent antioxidant effect.⁸⁹ Clinical Evidence and Challenges: Robust clinical trial data for NAFLD are lacking.⁹² A significant hurdle is the poor oral bioavailability of its key components,⁹² spurring the development of innovative co-delivery systems like metal-phenolic networks^93,94

Leaf-Derived Herbs

Artemisia annua extracts (scopoletin [SCO]) and artemisinin function through the LKB1/AMPK pathway.^95–97 Unique Mechanisms and Synergies: Artemisinin demonstrates a compelling multi-faceted immunomodulatory role by suppressing the NLRP3 inflammasome through the AMPK–NF-κB–NLRP3 pathway.⁹⁷ Clinical Evidence and Challenges: Clinical trials for NAFLD/AS are very limited.⁹⁸ Repurposing this antimalarial drug faces challenges: low bioavailability (19-35%), individual metabolic variations, and potential toxicity at high doses.⁹⁸

Ginkgolide C (GC) and Ginkgo biloba extract (GbE) act via LKB1/AMPK.^99,100 Unique Mechanisms and Synergies: GC additionally enhances mitochondrial function through the LKB1/AMPK–PGC-1α axis.⁹⁹ GbE's effects are further mediated via Akt/eNOS signaling and suppression of p38 mitogen-activated protein kinase (MAPK)/NF-κB.¹⁰⁰

Flower-Derived Herbs

Saffron-derived bioactive compounds (crocin, crocetin, safflower yellow A [SYA]) show efficacy via LKB1/AMPK.⁹⁰^101–103 Unique Mechanisms and Synergies: Crocetin engages the SIRT1/AMPK pathway to improve lipid profiles.¹⁰³ It also suppresses vascular inflammation by inhibiting NF-κB activation.^102,103 Clinical Evidence and Challenges: Clinical studies, particularly in coronary artery disease patients, show promise. Crocetin (10 mg/day) improved lipid profiles and homocysteine,¹⁰³ while crocin (30 mg/day) modulated gene expression (SIRT1/AMPK↑; lectin-type oxidized LDL receptor 1/NF-κB↓) and reduced ox-LDL.¹⁰⁴ Saffron is generally safe, but its dose optimization is complex due to the different potencies of its compounds.^103,105

These findings collectively indicate that natural products exert protective actions against NAFLD and AS by simultaneously modulating multiple targets and coordinating cross-organ crosstalk through the LKB1/AMPK pathway. A schematic overview of these multi-organ and multi-target mechanisms is illustrated in Figure 3.

Figure 3.

Multi-Target and multi-Organ Protective Mechanisms of Natural Products Against NAFLD and AS via the LKB1/AMPK Pathway. Annotations: SIRT1: Sirtuin 1; PGC-1α: Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-Alpha; mTOR: Mechanistic Target of Rapamycin; ACC: Acetyl-CoA Carboxylase; CPT1A: Carnitine Palmitoyltransferase 1A; SREBP1c: Sterol Regulatory Element-Binding Protein 1c; Hmgcs2: 3-Hydroxy-3-Methylglutaryl-CoA Synthase 2; HMGR: 3-Hydroxy-3-Methylglutaryl-CoA Reductase; eNOS: Endothelial Nitric Oxide Synthase; NF-κB: Nuclear Factor Kappa B; IL-6: Interleukin-6; TNF-α: Tumor Necrosis Factor-Alpha; ULK1: unc-51 Like Autophagy Activating Kinase 1; HO-1: Heme Oxygenase 1; PPARα: Peroxisome Proliferator-Activated Receptor Alpha; PPARγ: Peroxisome Proliferator-Activated Receptor Gamma; IRS: Insulin Receptor Substrate; FASN: Fatty Acid Synthase; SCD1: Stearoyl-CoA Desaturase-1; DGAT1: Diacylglycerol O-Acyltransferase 1; AP-1: Activator Protein 1; p53: Tumor Protein p53; p21: Cyclin-Dependent Kinase Inhibitor 1A; IGFBP1: Insulin-Like Growth Factor Binding Protein 1; TGFβ: Transforming Growth Factor beta; CTGF: Connective Tissue Growth Factor; Col1a1: Collagen Type I Alpha 1 Chain; NLRP3: NLR Family Pyrin Domain Containing 3; ICAM-1: Intercellular Adhesion Molecule 1; VCAM-1: Vascular Cell Adhesion Molecule 1; LXRα: Liver X Receptor Alpha; M1→M2 Polarization: Macrophage M1 to M2 Polarization; THC: Tetrahydrocurcumin; GC: Ginkgolide C; GbE: Ginkgo biloba Extract; SYA: Safflower Yellow A; SAA: Salvianolic Acid A; STS: Sodium Tanshinone IIA Sulfonate; RRP: Radix Rubiae Paeoniae; PA: Puerarin; SCO: Scopoletin. Created with BioRender.com.

Targeted Regulation of Monomeric Compounds

Polyphenolic Compound

Luteolin ameliorates pathology through the nicotinamide phosphoribosyltransferase (NAMPT)-Sirt1/AMPK axis.^106–108 Unique Mechanisms and Synergies: Its effects are significantly reinforced by its unique action of upregulating NAMPT–Sirt1 signaling, leading to reduced forkhead box protein O1 (FOXO1) acetylation and improved lipid metabolism.^106–108 Clinical Evidence and Challenges: Direct clinical evidence in NAFLD/AS is very limited.¹⁰⁹ A major challenge is that dietary intake is much lower than efficacious preclinical doses.¹⁰⁹

Baicalin (BAI) multi-dimensionally ameliorates pathology by targeting the LKB1/AMPK axis.^110–112 Unique Mechanisms and Synergies: BAI activates the Nrf2/HO-1 antioxidant pathway and inhibits NF-κB-mediated inflammation.^110,111 It also enhances insulin sensitivity via the AMPK–Akt/glycogen synthase kinase-3 beta (GSK-3β) axis.¹¹² Clinical Evidence and Challenges: There is a notable lack of clinical trials investigating baicalin for NAFLD or AS.¹¹³ Its clinical translation is primarily hampered by low oral bioavailability.¹¹³

Quercetin (Que) ameliorates pathology via the LKB1/AMPK axis.⁹⁷^114–116 Unique Mechanisms and Synergies: Que alleviates endoplasmic reticulum (ER) stress through the AMPK/protein phosphatase 2A (PP2A) axis.¹¹⁵ It also promotes macrophage M2 polarization and inhibits the M1 phenotype via Akt signaling.^114,116 Clinical Evidence and Challenges: An RCT in NAFLD patients showed that 500 mg/day quercetin for 12 weeks significantly reduced hepatic fat content (∼17.4%).¹¹⁷ Its low and variable bioavailability presents a significant challenge.^117,118

Xanthohumol (Xn) exerts therapeutic effects on NAFLD and AS by activating AMPK phosphorylation.^119,120 Unique Mechanisms and Synergies: Xn induces Nrf2 nuclear translocation, enhances antioxidant enzyme activity, and inhibits NF-κB-driven cytokine release.¹¹⁹ It further reduces MCP-1 expression.¹²⁰

Nobiletin (NOB) synergistically regulates lipid metabolism and inflammation via AMPK signaling.^121,122 Unique Mechanisms and Synergies: NOB activates IRS-1/AKT and SIRT1-related pathways, thereby enhancing glucose uptake and modulating inflammatory responses.¹²¹

EGCG exerts cross-disease therapeutic effects by activating LKB1/AMPK signaling.^123–125 Unique Mechanisms and Synergies: EGCG restores fibroblast growth factor 21 (FGF21) signaling sensitivity and activates the adiponectin receptor 2 (AdipoR2)/SIRT1 axis.^123,124 It also enhances antioxidative defenses via the Nrf2 pathway.¹²⁴ Clinical Evidence and Challenges: Clinical evidence for EGCG in NAFLD is limited and not yet conclusive.¹²⁶ Concerns about potential hepatotoxicity at high doses and its low bioavailability complicate dose optimization.^126,127

Resveratrol (RSV) exerts multi-target therapeutic effects by activating the SIRT1-dependent LKB1/AMPK axis.^128–135 Unique Mechanisms and Synergies: RSV promotes cholesterol efflux through the liver X receptor alpha (LXRα)/ABC-A1 axis.^129,130 It inhibits inflammation via NF-κB/TLR4 suppression and upregulates antioxidative defenses.^131–133 Clinical Evidence and Challenges: Clinical trial results are mixed. A meta-analysis found no significant effect on most NAFLD parameters,¹³⁶ while other RCTs show benefits.^137,138 Its very low oral bioavailability is a major limitation.^137,138

Gallic acid (GA) exerts multi-target therapeutic effects by activating the LKB1-dependent AMPK pathway.^139–141 Unique Mechanisms and Synergies: GA downregulates lipid uptake genes and enhances antioxidant defenses via the Nrf2/HO-1 axis.^139,140 In AS, GA improves endothelial function via AMPK–eNOS activation.^140,141 Clinical Evidence and Challenges: Clinical data for gallic acid in NAFLD or AS is currently lacking.¹⁴² Its development is challenged by high water solubility leading to low bioavailability.¹⁴²

Terpenoid Compound

Betulinic acid (BA) ameliorates NAFLD and AS by modulating the calcium/calmodulin-dependent protein kinase kinase–AMPK signaling axis.^143–145 Unique Mechanisms and Synergies: BA suppresses mTOR–ribosomal protein S6 kinase signaling and activates PGC-1α-associated thermogenesis.^143,144

Gastrodin exerts cross-disease regulatory effects through the LKB1/AMPK signaling axis.^146–148 Unique Mechanisms and Synergies: Gastrodin activates the Nrf2/HO-1 antioxidant pathway and inhibits NF-κB-mediated inflammation.^146–148 It also intervenes by inhibiting TGF-β/Smad2-driven fibrosis in NAFLD.¹⁴⁷

Alkaloid Compound

Berberine (BBR) is a premier example of a natural product with cross-disease efficacy through the LKB1/AMPK signaling axis.^149–155 Unique Mechanisms and Synergies: Uniquely, a significant part of BBR's metabolic benefit originates from its ability to modulate gut microbiota and bile acid metabolism, a systemic effect that indirectly reinforces AMPK activation.^104,106 Clinical Evidence and Challenges: Berberine is standout for its strong clinical validation. Multiple RCTs confirm its efficacy in improving liver enzymes, lipid profiles, and IR in NAFLD.^156,157 The primary challenge remains its low bioavailability, motivating research into novel delivery systems and combination therapies.^156,158,159

Glycoside Compound

Salidroside (SAL) exerts pleiotropic therapeutic effects by targeting the LKB1/AMPK signaling axis.^160–162 Unique Mechanisms and Synergies: SAL additionally regulates oxidative stress and inflammation by modulating the NLRP3 inflammasome.^160,161

Nucleoside Compound

Cordycepin ameliorates pathology through multi-target modulation of the LKB1/AMPK signaling axis.^163,164 Unique Mechanisms and Synergies: Beyond direct AMPK activation, Cordycepin reduces tumor necrosis factor (TNF) signaling activity and inhibits TGF-β/Smad-driven fibrotic responses.¹⁶³

Phenolic Derivative

6-Gingerol exerts pleiotropic therapeutic effects through the LKB1/AMPK signaling axis.^165–167 Unique Mechanisms and Synergies: 6-Gingerol reduces oxidative stress and inflammation.¹⁶⁵ In AS, it alleviates vascular inflammation via NF-κB pathway inhibition and suppresses macrophage pyroptosis.^166,167

Representative structures of these compounds are shown in Figure 4, and their classifications and mechanisms of action are summarized in Table 1.

Figure 4.

Bioactive Compounds Structures. (1) Luteolin; (2) Baicalin; (3) Quercetin; (4) Xanthohumol; (5) Nobiletin; (6) Epigallocatechin Gallate; (7) Resveratrol; (8) Gallic Acid; (9) Betulinic Acid; (10) Gastrodin; (11) Berberine; (12) Salidroside; (13)Cordycepin; (14) 6-Gingerol.

Table 1.

Comprehensive Summary of Natural Products Targeting the LKB1/AMPK Pathway and Associated Mechanisms in NAFLD and AS Therapy.

Chemical Compound (Source)	Direct AMPK-Mediated Mechanisms (LKB1/AMPK Axis)	Key Secondary/Synergistic Pathways & Mechanisms	Disease Model	Evidence Type (Selected Models)	References
Tetrahydrocurcumin (Curcuma longa)	Activates LKB1/AMPK; suppresses SREBP-1c; promotes PPARα/CPT-1a-mediated FAO.	Improves insulin sensitivity via IRS–PI3K–Akt signaling.	NAFLD	in vivo (HFD-mice)	⁶⁷
Curcumin (Curcuma longa)	Activates AMPK–SIRT1–LXRα axis to promote cholesterol efflux in macrophages.	Suppresses NF-κB via AMPK–PKC–MAPK; inhibits EMMPRIN/MMP to stabilize plaques.	AS	in vitro (THP-1 cells)	^69–71
Ginsenoside Rg5 (Panax ginseng)	Activates LKB1/AMPK to suppress SREBP-1c/FASN.	Modulates metabolism through LKB1/AMPK/mTOR axis; improves gut barrier function.	NAFLD	in vivo (HFD-mice)	^72,77
Ginsenoside CK (Panax ginseng)	Activates LKB1/AMPK to downregulate SREBP-1c and promote PPARα/CPT1α-driven FAO.	N/A	NAFLD	in vitro (HepG2); in vivo (Fructose-mice)	^74,78
Ginsenoside Rg3 (Panax ginseng)	Inhibits VSMC proliferation via direct LKB1/AMPK signaling.	Promotes macrophage M2 polarization through PPARγ activation.	AS	in vitro (BMDMs); in vivo (ApoE⁻/⁻ mice)	^75,79
Salvianolic acid A (Salvia miltiorrhiza)	Regulates lipid homeostasis via LKB1/AMPK/IGFBP1 axis.	Uniquely inhibits ferroptosis; enhances mitochondrial function.	NAFLD	in vitro (AML-12); in vivo (ApoE⁻/⁻ mice)	⁸⁷
Sodium Tanshinone IIA Silate (Salvia miltiorrhiza)	Suppresses VSMC proliferation via AMPK/p53/p21 axis.	Activates AMPK/Nrf2/HO-1 pathway to attenuate oxidative stress and inflammation.	AS	in vitro (VSMCs); in vivo (Rat MI model)	⁸⁹
Artemisinin (Artemisia annua)	Enhances macrophage autophagy via AMPK–ULK1 signaling.	Suppresses NLRP3 inflammasome activation via AMPK–NF-κB pathway.	AS	in vitro (RAW264.7); in vivo (ApoE⁻/⁻ mice)	^96,97
Luteolin	Activates AMPK pathway; promotes p-ACC; activates PPARα-driven oxidation.	Upregulates NAMPT–Sirt1 signaling; inhibits NF-κB.	NAFLD, AS	in vitro (HepG2, THP-1); in vivo (HFD-mice)	^106–108
Baicalin	Stabilizes LKB1/AMPK complex; promotes AMPK phosphorylation; suppresses SREBP1.	Activates Nrf2/HO-1 pathway; inhibits NF-κB; enhances insulin sensitivity via AMPK–Akt/GSK-3β.	NAFLD, AS	in vitro (HepG2, AML12); in vivo (HFD-mice)	^110–112
Quercetin	Activates LKB1/AMPK signaling; inhibits ACACA; attenuates lipogenesis.	Alleviates ER stress via AMPK/PP2A; promotes M2 macrophage polarization via Akt.	NAFLD, AS	in vitro (HepG2, VSMCs, RAW264.7); in vivo (HFD-mice); Clinical (RCT, NAFLD)	⁹⁷ ^114–117
EGCG	Activates LKB1 phosphorylation & AMPK signaling; suppresses lipogenesis.	Restores FGF21 sensitivity; activates AdipoR2/SIRT1; enhances Nrf2 defenses; protects endothelium via SIRT1–AMPK–Akt/eNOS.	NAFLD, AS	in vitro (Hepatocytes, HUVECs); in vivo (HFD-mice)	^123–125
Resveratrol	Activates SIRT1-dependent LKB1/AMPK axis; increases p-ACC; enhances β-oxidation.	Promotes cholesterol efflux via LXRα/ABC-A1; inhibits inflammation via NF-κB/TLR4; induces autophagy; reduces ER stress.	NAFLD, AS	in vitro, in vivo; Clinical (Mixed results)	^128–131 ^,136–138
Gallic acid	Activates LKB1-dependent AMPK pathway; suppresses lipogenic factors. Direct binder of AMPKα/β subunits.	Enhances antioxidant defenses via Nrf2/HO-1; suppresses inflammation; improves endothelial function via AMPK–eNOS.	NAFLD, AS	in vitro (HepG2, A7r5 cells)	^139–141
Berberine	Enhances LKB1/AMPK complex stability; suppresses SREBP-1c & ACC; promotes LDLR-mediated clearance.	Modulates gut microbiota; activates SIRT3/AMPK & AMPK/NRF1; suppresses NF-κB/YY1.	NAFLD, AS	in vitro, in vivo; Clinical (RCTs)	^149–155 ^,156–159
Salidroside	Activates LKB1/AMPK axis (via miR-802/PRKAA1); inhibits lipogenesis; improves endothelial function via LKB1/AMPK–PI3 K/Akt/eNOS.	Modulates NLRP3 inflammasome; mitigates oxidative stress (partly AMPK-independent).	NAFLD, AS	in vitro (Hepatocytes, HUVECs); in vivo (HFD, ApoE⁻/⁻ mice)	^160–162
Cordycepin	Activates LKB1/AMPK pathway; suppresses lipogenesis; enhances macrophage autophagy via AMPK.	Reduces TNF signaling; inhibits TGF-β/Smad fibrosis; involves mTOR suppression in autophagy.	NAFLD, AS	in vitro (L02 cells, RAW264.7); in vivo (HFD, ApoE⁻/⁻ mice)	^163,164
6-Gingerol	Directly binds & stabilizes LKB1/STRAD/MO25 complex; activates LKB1/AMPK; inhibits lipogenesis; promotes cholesterol efflux.	Reduces oxidative stress & inflammation; inhibits NF-κB; suppresses macrophage pyroptosis; enhances eNOS via AMPK.	NAFLD, AS	in vitro (HepG2); in vivo (HFD, ApoE⁻/⁻ mice)	^136–138 ^,165–167

Pharmacological and Translational Challenges of Natural Products Targeting the LKB1/AMPK Pathway

Bioavailability Limitations

Despite promising efficacy in preclinical models, many natural products modulating the LKB1/AMPK pathway suffer from low oral bioavailability. Poor aqueous solubility, rapid phase II metabolism, and high polarity (eg, polyphenols, ginsenosides, phenolic acids) restrict systemic exposure.^86,168,169 For example, curcumin shows negligible plasma levels after oral administration due to poor solubility and rapid metabolism,^170,171 while many ginsenosides exhibit oral bioavailability often <1%–5%.^172,173 These properties necessitate supraphysiological doses in animal studies and complicate dose selection for human trials.

Pharmacokinetics and Metabolism

The pharmacokinetic (PK) behavior of LKB1/AMPK-activating compounds is complex and variable. Absorption is strongly influenced by formulation and route of administration.^169,174 Distribution is further affected by plasma protein binding and tissue affinity.^175,176 Many compounds undergo rapid hepatic metabolism and conjugation, or are transformed by gut microbiota into secondary metabolites with distinct activity.^177–179 Elimination pathways, including biliary and renal excretion, often shorten half-life (eg, artemisinin) and limit sustained exposure.^180–182 In addition, drug–drug or nutrient–drug interactions (eg, with B vitamins or chemotherapeutics) can markedly alter systemic exposure.^183–186

Species and Disease-State Variability

Interspecies differences in absorption, metabolizing enzymes, transporter expression, and microbiota composition lead to divergent pharmacokinetics and pharmacodynamics (PD) between rodents and humans.^170,171 For instance, ginsenoside metabolite profiles and time to maximum concentration differ substantially between mice and rats.^178,182 Moreover, nanoformulations that significantly increase maximum concentration/area under the curve in rodents often fail to achieve comparable exposure in humans.¹⁸⁷ Disease conditions such as NAFLD and AS further influence absorption and metabolism, complicating extrapolation of preclinical efficacy to patients.

Translation Gaps from Animal to Human Studies

Natural products such as resveratrol, EGCG, and xanthohumol robustly activate LKB1/AMPK in experimental models.^86,170,175 However, clinical trials have yielded mixed or modest outcomes.^188,189 Discrepancies often arise from insufficient systemic exposure in humans compared with animal studies,¹⁹⁰ differences in active metabolite formation,¹⁹¹ and reliance on simplified animal models that fail to capture the heterogeneity of human NAFLD and AS.¹⁹²

Future Perspectives

To overcome these barriers, future studies should integrate rigorous PK/PD characterization, including metabolite profiling.^175,188 Optimization of delivery systems and use of structural analogs are needed to enhance systemic exposure.¹⁹⁰ Physiologically based pharmacokinetic modeling can help bridge preclinical and clinical data.¹⁹¹ Furthermore, early clinical trials should ensure that human exposure levels approximate those producing efficacy in animal models.¹⁹² These strategies are essential to harness the therapeutic potential of natural products acting through the LKB1/AMPK pathway.

Discussion

LKB1/AMPK Signaling Hub: A Tripartite Regulatory Core Integrating Metabolism, Inflammation, and Mitochondria

LKB1/AMPK pathway acts as a “central processor” of energy metabolism, orchestrating multi-dimensional regulatory roles in the comorbidity of NAFLD and AS by integrating pathological networks between the hepatic and vascular systems. From a mechanistic perspective, AMPK bidirectionally regulates lipid metabolism by phosphorylating ACC (inhibiting lipid synthesis) and activating PPARα/CPT1a (promoting fatty acid β-oxidation), thereby breaking hepatic lipotoxicity cycles. Concurrently, it enhances macrophage cholesterol efflux via ABC-A1/ABC-G1 upregulation, suppressing AS plaque core formation.^36,38,39 Tissue-specific regulation is also evident: hypothalamic LKB1 activates the AMPK-POMC neuron-sympathetic axis to induce white adipose browning and suppress hepatic SREBP1c expression,^51,53,54 while intestinal LKB1 deficiency disrupts mitochondrial FAO, leading to lipid accumulation.⁴⁴

In addition to metabolic control, AMPK exerts potent anti-inflammatory effects through mTORC1/NF-κB inhibition and SIRT1-mediated p65 deacetylation, while reshaping the Th17/Treg immune balance.^55,59,61 On the mitochondrial side, the LKB1/AMPK-PGC-1α network drives mitochondrial biogenesis and Unc-51 like autophagy activating kinase 1 (ULK1)-mediated mitophagy, while alleviating ROS-mediated vascular endothelial injury.^43,52,63 Collectively, this “metabolism–inflammation–mitochondria” triad establishes a mechanistic foundation for therapies targeting LKB1/AMPK.

Liver–Vascular Axis Crosstalk: Exosomal miRNA-Mediated Communication

The vicious cycle of the liver–vascular axis is not solely dependent on classical circulating lipids or cytokines, but also mediated through exosomal microRNAs (miRNAs). Hepatocyte-derived small extracellular vesicle (sEV)-miR-30a-3p suppresses ABC-A1 expression, reducing macrophage cholesterol efflux and promoting coronary plaque formation.³⁵ Conversely, AS plaque-derived exosomes carrying receptor-interacting protein kinase 1 messenger RNA (mRNA) activate hepatic NLRP3 inflammasomes, accelerating NAFLD progression.²⁸ This bidirectional communication establishes a “hepatic lipotoxicity → vascular inflammation → hepatic fibrosis” feedback loop.

The LKB1/AMPK pathway may disrupt such pathological signaling by modulating exosomal cargo (eg, reducing miR-30a-3p secretion) and restoring mitochondrial function.^35,61 Emerging interventions such as curcumin nanoparticles and RSV exemplify how natural compounds, acting through LKB1/AMPK activation, remodel inter-organ communication and immune microenvironments.^{67,71,107,193}

Multi-Target Intervention by Natural Products: From Molecular Synergy to Clinical Translation Challenges

Natural products, with their multi-component and multi-target characteristics, hold unique promise in NAFLD-AS comorbidity. For example, ginsenoside Rg5 suppresses hepatic lipogenesis via the AMPK/mTOR axis while promoting FAO, STS activates both the AMPK/p53/p21 and Nrf2/HO-1 pathways, BBR exhibits liver-targeting with vascular benefits, and RSV simultaneously induces autophagy and improves endothelial function.^{69,70,72,79,106,107}^122–124^,193

Despite these advances, clinical translation remains hindered by poor bioavailability, insufficient organ selectivity, and unresolved synergy among multiple components. New technologies provide potential solutions:

Nanocarriers improve solubility, bioavailability, and tissue targeting (eg, curcumin- or ginsenoside-loaded nanoparticles).^170,171

Gut microbiota modulation leverages host–microbiome interactions to indirectly enhance AMPK activation.^104,106

Structural modifications and bioenhancer combinations increase systemic exposure and therapeutic efficacy.^179,191

Conclusion and Limitations

In conclusion, the LKB1/AMPK pathway integrates metabolic, inflammatory, and mitochondrial regulation, forming a central hub in the progression of NAFLD-AS comorbidity. Exosomal miRNA-mediated liver–vascular communication further amplifies disease burden, while natural products—through their multi-target and systemic regulatory potential—offer promising therapeutic opportunities.

However, several limitations must be acknowledged. Current evidence is largely derived from preclinical studies with heterogeneous models, and pharmacokinetic and pharmacodynamic profiles of many natural products remain insufficiently characterized. Moreover, the precise interactions among multi-component herbal preparations are still poorly understood.

Looking forward, emerging technologies such as nanocarrier-based delivery systems, gut microbiota modulation, and rational structural modification represent transformative strategies to overcome the long-standing barriers of bioavailability and organ specificity. By integrating these approaches, natural product–based interventions targeting the LKB1/AMPK axis may ultimately achieve successful clinical translation and provide novel therapeutic solutions for patients with NAFLD-AS comorbidity.

Footnotes

Abbreviations

ORCID iD

Jian-Ping Zhu

Ethical Approval

Ethical Approval is not applicable for this article.

Author Contributions

Conceptualization, J.-P.Z. and J.-X.K.; writing—original draft preparation, J.-X.K.; writing—review and editing, J.-X.K.; visualization, T.W.; supervision, H.C.; project administration, J.-P.Z.; funding acquisition, J.-P.Z. All authors have read and agreed to the published version of the manuscript.

Funding

The authors disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was supported by the Hunan Provincial Training Program for Young Core Teachers in Higher Education Institutions, University-level Scientific Research Project Funded by Hunan University of Chinese Medicine, Scientific Research Project Funded by Hunan Provincial Department of Education, (grant number 2024XJZC017, 23B0375).

Declaration of Conflicting Interests

The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Data Availability Statement

All data related to this study are presented in this publication.

Statement of Human and Animal Rights

This article does not contain any studies with human or animal subjects.

Statement of Informed Consent

There are no human subjects in this article and informed consent is not applicable.

References

Miao

Targher

Byrne

Cao

Zheng

. Current status and future trends of the global burden of MASLD. Trends Endocrinol. Metab. 2024;35(8):697‐707. https://doi.org/10.1016/j.tem.2024.02.007

Driessen

Francque

Anker

, et al. Metabolic dysfunction-associated steatotic liver disease and the heart. Hepatology. 2025;82(2):487‐503. https://doi.org/10.1097/HEP.0000000000000735

Koskinas

Van Craenenbroeck

Antoniades

, et al. Obesity and cardiovascular disease: an ESC clinical consensus statement. Eur. Heart J. 2024;45(38):4063‐4098. https://doi.org/10.1093/eurheartj/ehae508

Rasool

Dar

Latief

Dar

Sofi

Khan

. Nonalcoholic fatty liver disease as an independent risk factor for carotid atherosclerosis. Brain Circ. 2017;3(1):35-40. https://doi.org/10.4103/bc.bc_28_16

Jamalinia

Zare

Lankarani

. Systematic review and meta-analysis: association between liver fibrosis and subclinical atherosclerosis in nonalcoholic fatty liver disease. Aliment. Pharmacol. Ther. 2023;58(4):384‐394. https://doi.org/10.1111/apt.17617

Gill

Vatcheva

Pan

, et al. Frequency of nonalcoholic fatty liver disease and subclinical atherosclerosis among young Mexican Americans. Am. J. Cardiol. 2017;119(11):1717‐1722. https://doi.org/10.1016/j.amjcard.2017.03.010

Han

Wang

Yuan

, et al. Nonalcoholic fatty liver disease represents a greater metabolic burden in patients with atherosclerosis: a cross-sectional study. Medicine (Baltimore). 2019;98(11):e14896. https://doi.org/10.1097/MD.0000000000014896

Abdallah

de Matos

Souza

YPDM

Vieira-Soares

Muller-Machado

Pollo-Flores

. Non-alcoholic fatty liver disease and its links with inflammation and atherosclerosis. Curr. Atheroscler. Rep. 2020;22(1):7. https://doi.org/10.1007/s11883-020-0820-8

Kang

Kim

. Nonalcoholic fatty liver disease is associated with the presence and morphology of subclinical coronary atherosclerosis. Yonsei Med. J. 2015;56(5):1288‐1295. https://doi.org/10.3349/ymj.2015.56.5.1288

10.

Zeb

Budoff

, et al. Nonalcoholic fatty liver disease and incident cardiac events: the multi-ethnic study of atherosclerosis. J. Am. Coll. Cardiol. 2016;67(16):1965‐1966. https://doi.org/10.1016/j.jacc.2016.01.070

11.

Stols-Gonçalves

Hovingh

Nieuwdorp

Holleboom

. NAFLD And atherosclerosis: two sides of the same dysmetabolic coin? Trends Endocrinol. Metab. 2019;30(12):891‐902. https://doi.org/10.1016/j.tem.2019.08.008

12.

Zhang

Zheng

Lei

, et al. A clinical study of the correlation between metabolic-associated fatty liver disease and coronary plaque pattern. Sci. Rep. 2023;13(1):7224. https://doi.org/10.1038/s41598-023-34462-8

13.

Zheng

Zhou

Zhang

, et al. Association between nonalcoholic fatty liver disease and subclinical atherosclerosis: a cross-sectional study on population over 40 years old. BMC Cardiovasc. Disord. 2018;18(1):147. https://doi.org/10.1186/s12872-018-0877-2

14.

Dogru

Genc

Tapan

, et al. Plasma fetuin-A is associated with endothelial dysfunction and subclinical atherosclerosis in subjects with nonalcoholic fatty liver disease. Clin. Endocrinol. 2013;78(5):712‐717. https://doi.org/10.1111/j.1365-2265.2012.04460.x

15.

Simon

Trejo

MEP

McClelland

, et al. Circulating interleukin-6 is a biomarker for coronary atherosclerosis in nonalcoholic fatty liver disease: results from the multi-ethnic study of atherosclerosis. Int. J. Cardiol. 2018;259(1):198‐204. https://doi.org/10.1016/j.ijcard.2018.01.046

16.

Yari

Shabani

Karami

Sarmadi

Poustchi

Bandegi

. Circulating levels of FAM19A5 are inversely associated with subclinical atherosclerosis in non-alcoholic fatty liver disease. BMC Endocr. Disord. 2021;21(1):153. https://doi.org/10.1186/s12902-021-00820-8

17.

DeFilippis

Blaha

Martin

, et al. Nonalcoholic fatty liver disease and serum lipoproteins: the multi-ethnic study of atherosclerosis. Atherosclerosis. 2013;227(2):429‐436. https://doi.org/10.1016/j.atherosclerosis.2013.01.022

18.

Sohrabi

Reinecke

. RIPK1 Targeting protects against obesity and atherosclerosis. Trends Endocrinol. Metab. 2021;32(7):420‐422. https://doi.org/10.1016/j.tem.2021.03.009

19.

Chen

Pang

, et al. Hepatic steatosis aggravates atherosclerosis via small extracellular vesicle-mediated inhibition of cellular cholesterol efflux. J. Hepatol. 2023;79(6):1491‐1501. https://doi.org/10.1016/j.jhep.2023.08.023

20.

Wang

Liu

Wang

, et al. Natural active botanical metabolites: targeting AMPK signaling pathway to treat metabolic dysfunction-associated fatty liver disease. Front Pharmacol. 2025;16(1):1611400. https://doi.org/10.3389/fphar.2025.1611400

21.

Omidkhoda

Mahdiani

Hayes

Karimi

. Natural compounds against nonalcoholic fatty liver disease: a review on the involvement of the LKB1/AMPK signaling pathway. Phytother Res. 2023;37(12):5769‐5786. https://doi.org/10.1002/ptr.8020

22.

Wang

Yan

Peng

. Natural products in non-alcoholic fatty liver disease (NAFLD): novel lead discovery for drug development. Pharmacol Res. 2023;196(1):106925. https://doi.org/10.1016/j.phrs.2023.106925

23.

Patel

Siddiqui

. The interplay between nonalcoholic fatty liver disease and atherosclerotic heart disease. Hepatology. 2019;69(1):1372‐1374. https://doi.org/10.1002/hep.30410

24.

Jiang

Chen

Wang

Ling

Yan

Xia

. Hepatocyte-derived extracellular vesicles promote endothelial inflammation and atherogenesis via microRNA-1. J. Hepatol. 2020;72(1):156‐166. https://doi.org/10.1016/j.jhep.2019.09.014

25.

Wang

Yang

Zhu

. AMPK Activation enhances the anti-atherogenic effects of high density lipoproteins in apoE-/- mice. J. Lipid Res. 2017;58(8):1536‐1547. https://doi.org/10.1194/jlr.M073270

26.

Wang

Liang

Xiang

Chen

. LKB1 Regulates vascular macrophage functions in atherosclerosis. Front. Pharmacol. 2021;12(1):810224. https://doi.org/10.3389/fphar.2021.810224

27.

Vasamsetti

Karnewar

Gopoju

, et al. Resveratrol attenuates monocyte-to-macrophage differentiation and associated inflammation via modulation of intracellular GSH homeostasis: relevance in atherosclerosis. Free Radic. Biol. Med. 2016;96(1):392‐405. https://doi.org/10.1016/j.freeradbiomed.2016.05.003

28.

Gaudio

Nobili

Franchitto

Onori

Carpino

. Nonalcoholic fatty liver disease and atherosclerosis. Intern. Emerg. Med. 2012;7(1):S297‐S305. https://doi.org/10.1007/s11739-012-0826-5

29.

Kucukazman

Ata

Yavuz

, et al. Evaluation of early atherosclerosis markers in patients with nonalcoholic fatty liver disease. Eur. J. Gastroenterol. Hepatol. 2013;25(2):147‐151. https://doi.org/10.1097/MEG.0b013e32835a58b1

30.

Dong

, et al. Research advances in the relationship between nonalcoholic fatty liver disease and atherosclerosis. Lipids Health Dis. 2015;14(1):158. https://doi.org/10.1186/s12944-015-0141-z

31.

Han

Wen

Pan

Chen

. The association between atherosclerosis and nonalcoholic fatty liver disease. Medicine (Baltimore). 2024;103(1):e36815. https://doi.org/10.1097/MD.0000000000036815

32.

Gao

Chen

Zhu

. A potential strategy for treating atherosclerosis: improving endothelial function via AMP-activated protein kinase. Sci. China Life Sci. 2018;61(9):1024‐1029. https://doi.org/10.1007/s11427-017-9285-1

33.

Huangfu

Huang

Liao

Zou

Shang

. M1 linear ubiquitination of LKB1 inhibits vascular endothelial cell injury in atherosclerosis through activation of AMPK. Hum. Cell. 2023;36(6):1901‐1914. https://doi.org/10.1007/s13577-023-00950-2

34.

Stachowicz

Wiśniewska

Kuś

, et al. Diminazene aceturate stabilizes atherosclerotic plaque and attenuates hepatic steatosis in apoE-knockout mice by influencing macrophages polarization and taurine biosynthesis. Int. J. Mol. Sci. 2021;22(11):5861. https://doi.org/10.3390/ijms22115861

35.

Zhao

Kong

Cui

, et al. Communication between nonalcoholic fatty liver disease and atherosclerosis: focusing on exosomes. Eur. J. Pharm. Sci. 2024;193(1):106690. https://doi.org/10.1016/j.ejps.2024.106690

36.

Luo

Saha

Xiang

Ruderman

. AMPK, the metabolic syndrome and cancer. Trends Pharmacol. Sci. 2005;26(2):69‐76. https://doi.org/10.1016/j.tips.2004.12.011

37.

Yan

Zhou

Melcher

. Structure and physiological regulation of AMPK. Int. J. Mol. Sci. 2018;19(11):3534. https://doi.org/10.3390/ijms19113534

38.

Carling

. The AMP-activated protein kinase cascade–a unifying system for energy control. Trends Biochem. Sci. 2004;29(1):18‐24. https://doi.org/10.1016/j.tibs.2003.11.005

39.

Foretz

Taleux

Guigas

, et al. Régulation du métabolisme énergétique par l'AMPK: une nouvelle voie thérapeutique pour le traitement des maladies métaboliques et cardiaques. Med. Sci. 2006;22(4):381‐388. https://doi.org/10.1051/medsci/2006224381

40.

Kottakis

Bardeesy

. LKB1-AMPK Axis revisited. Cell Res. 2012;22(12):1617‐1620. https://doi.org/10.1038/cr.2012.108

41.

Al-Rasheed

Attia

, et al. Renoprotective effects of fenofibrate via modulation of LKB1/AMPK mRNA expression and endothelial dysfunction in a rat model of diabetic nephropathy. Pharmacology. 2015;95(5-6):229‐239. https://doi.org/10.1159/000381190

42.

Shackelford

Shaw

. The LKB1-AMPK pathway: metabolism and growth control in tumour suppression. Nat. Rev. Cancer. 2009;9(8):563‐575. https://doi.org/10.1038/nrc2676

43.

Jiang

Wang

Luo

, et al. AMP-activated protein kinase regulates cancer cell growth and metabolism via nuclear and mitochondria events. J. Cell. Mol. Med. 2019;23(6):3951‐3961. https://doi.org/10.1111/jcmm.14279

44.

Gao

Yan

Tripathi

, et al. LKB1 Represses ATOH1 via PDK4 and energy metabolism and regulates intestinal stem cell fate. Gastroenterology. 2020;158(5):1389‐1401. https://doi.org/10.1053/j.gastro.2019.12.033

45.

Bourouh

Marignani

. The tumor suppressor kinase LKB1: metabolic nexus. Front. Cell Dev. Biol. 2022;10(1):881297. https://doi.org/10.3389/fcell.2022.881297

46.

Meng

Zhang

Tang

. Gradual deterioration of fatty liver disease to liver cancer via inhibition of AMPK signaling pathways involved in energy-dependent disorders, cellular aging, and chronic inflammation. Front. Oncol. 2023;13(1):1099624. https://doi.org/10.3389/fonc.2023.1099624

47.

Saravia

Raynor

Chapman

Lim

Chi

. Signaling networks in immunometabolism. Cell Res. 2020;30(4):328‐342. https://doi.org/10.1038/s41422-020-0301-1

48.

Xie

Yuan

, et al. Downregulation of hepatic ceruloplasmin ameliorates NAFLD via SCO1-AMPK-LKB1 complex. Cell Rep. 2022;41(3):111498. https://doi.org/10.1016/j.celrep.2022.111498

49.

Liu

Zhang

Wang

, et al. Pharmacological targeting of AMPK to restore glucose and fatty acid metabolism homeostasis attenuates transplanted kidney fibrosis. Biochim. Biophys. Acta Mol. Basis Dis. 2025;1871(1):167510. https://doi.org/10.1016/j.bbadis.2024.167510

50.

Jang

Choi

Hur

Kim

Kwon

. New insights into AMPK, as a potential therapeutic target in metabolic dysfunction-associated steatotic liver disease and hepatic fibrosis. Biomol. Ther. 2025;33(1):18‐38. https://doi.org/10.4062/biomolther.2024.188

51.

van der Vaart

Boon

Houtkooper

. The role of AMPK signaling in brown adipose tissue activation. Cells. 2021;10(4):1122. https://doi.org/10.3390/cells10051122

52.

Jiang

Liao

, et al. High phosphate impairs arterial endothelial function through AMPK-related pathways in mouse resistance arteries. Acta Physiol. 2021;231(4):e13595. https://doi.org/10.1111/apha.13595

53.

Zhang

, et al. LKB1 up-regulation inhibits hypothalamic inflammation and attenuates diet-induced obesity in mice. Metabolism. 2021;116(1):154694. https://doi.org/10.1016/j.metabol.2020.154694

54.

Liu

Guan

Zhou

Huang

. LKB1 Alleviates high glucose- and high fat-induced inflammation and the expression of GnRH and sexual precocity-related genes, in mouse hypothalamic cells by activating the AMPK/FOXO1 signaling pathway. Mol. Med. Rep. 2022;25(4):143. https://doi.org/10.3892/mmr.2022.12659

55.

van der Zande

Brombacher

Lambooij

, et al. Dendritic cell-intrinsic LKB1-AMPK/SIK signaling controls metabolic homeostasis by limiting the hepatic Th17 response during obesity. JCI Insight. 2023;8(5):e157948. https://doi.org/10.1172/jci.insight.157948

56.

Arad

Seidman

. AMP-activated protein kinase in the heart: role during health and disease. Circ. Res. 2007;100(4):474‐488. https://doi.org/10.1161/01.RES.0000258446.23525.37

57.

Tsai

Huang

Kao

, et al. Aspirin attenuates vinorelbine-induced endothelial inflammation via modulating SIRT1/AMPK axis. Biochem. Pharmacol. 2014;88(2):189‐200. https://doi.org/10.1016/j.bcp.2013.12.005

58.

Wang

Huang

Cao

, et al. Loureirin B reduces insulin resistance and chronic inflammation in a rat model of polycystic ovary syndrome by upregulating GPR120 and activating the LKB1/AMPK signaling pathway. Int. J. Mol. Sci. 2024;25(20):11146. https://doi.org/10.3390/ijms252011146

59.

Ding

Zhou

Ling

, et al. Metformin in cardiovascular diabetology: a focused review of its impact on endothelial function. Theranostics. 2021;11(19):9376‐9396. https://doi.org/10.7150/thno.64706

60.

Hyun

Kim

Park

, et al. Protective mechanisms of loquat leaf extract and ursolic acid against diabetic pro-inflammation. J. Mol. Med. 2022;100(10):1455‐1464. https://doi.org/10.1007/s00109-022-02243-x

61.

Baixauli

Piletic

Puleston

, et al. An LKB1-mitochondria axis controls TH17 effector function. Nature. 2022;610(7932):555‐561. https://doi.org/10.1038/s41586-022-05264-1

62.

Xiao

Wang

Luo

, et al. Lkb1 orchestrates γδ T-cell metabolic and functional fitness to control IL-17-mediated autoimmune hepatitis. Cell. Mol. Immunol. 2024;21(6):546‐560. https://doi.org/10.1038/s41423-024-01163-9

63.

Chen

Wang

, et al. Docosahexaenoic acid ester of phloridzin reduces inflammation and insulin resistance via AMPK. Curr. Pharm. Des. 2022;28(22):1854‐1862. https://doi.org/10.2174/1381612828666220518102440

64.

Huang

Zhan

, et al. Resveratrol-induced Sirt1 phosphorylation by LKB1 mediates mitochondrial metabolism. J. Biol. Chem. 2021;297(2):100929. https://doi.org/10.1016/j.jbc.2021.100929

65.

Rabinovitch

Samborska

Faubert

, et al. AMPK Maintains cellular metabolic homeostasis through regulation of mitochondrial reactive oxygen Species. Cell Rep. 2017;21(1):1‐9. https://doi.org/10.1016/j.celrep.2017.09.026

66.

Sun

Zhang

, et al. Metabolic regulator LKB1 controls adipose tissue ILC2 PD-1 expression and mitochondrial homeostasis to prevent insulin resistance. Immunity. 2024;57(6):1289‐1305. https://doi.org/10.1016/j.immuni.2024.04.024

67.

Pan

Chen

Kong

Lai

. Attenuation by tetrahydrocurcumin of adiposity and hepatic steatosis in mice with high-fat-diet-induced obesity. J. Agric. Food Chem. 2018;66(48):12685‐12695. https://doi.org/10.1021/acs.jafc.8b04624

68.

Lee

Nam

Yoon

, et al. Fermented Curcuma longa L. Prevents alcoholic fatty liver disease in mice by regulating CYP2E1, SREBP-1c, and PPAR-α. J. Med. Food. 2022;25(4):456‐463. https://doi.org/10.1089/jmf.2021.K.0098

69.

Lin

Liu

, et al. Curcumin enhanced cholesterol efflux by upregulating ABCA1 expression through AMPK-SIRT1-LXRα signaling in THP-1 macrophage-derived foam cells. DNA Cell Biol. 2015;34(9):561‐572. https://doi.org/10.1089/dna.2015.2866

70.

Soltani

Salmaninejad

Jalili-Nik

, et al. 5'-Adenosine Monophosphate-Activated protein kinase: a potential target for disease prevention by curcumin. J. Cell. Physiol. 2019;234(3):2241‐2251. https://doi.org/10.1002/jcp.27192

71.

Cao

Han

Tian

, et al. Curcumin inhibits EMMPRIN and MMP-9 expression through AMPK-MAPK and PKC signaling in PMA induced macrophages. J. Transl. Med. 2014;12(1):266. https://doi.org/10.1186/s12967-014-0266-2

72.

Saadati

Sadeghi

Mansour

, et al. Curcumin and inflammation in non-alcoholic fatty liver disease: a randomized, placebo controlled clinical trial. BMC Gastroenterol. 2019;19(1):133. https://doi.org/10.1186/s12876-019-1055-4

73.

Jabczyk

Nowak

Hudzik

Zubelewicz-Szkodzińska

. Curcumin in metabolic health and disease. Nutrients. 2021;13(12):4440. https://doi.org/10.3390/nu13124440

74.

Zhang

Deng

Tang

Guan

Chen

Fan

. Zingiberaceae plants/curcumin consumption and multiple health outcomes: an umbrella review of systematic reviews and meta-analyses of randomized controlled trials in humans. Phytother. Res. 2022;36(8):3080‐3101. https://doi.org/10.1002/ptr.7500

75.

Pillai

Borah

MNT

Kawano

Hasegawa

Kumar

. Co-Delivery of curcumin and bioperine via PLGA nanoparticles to prevent atherosclerotic foam cell formation. Pharmaceutics. 2021;13(9):1420. https://doi.org/10.3390/pharmaceutics13091420

76.

Laurindo

de Carvalho

de Oliveira Zanuso

, et al. Curcumin-Based nanomedicines in the treatment of inflammatory and immunomodulated diseases: an evidence-based comprehensive review. Pharmaceutics. 2023;15(1):229. https://doi.org/10.3390/pharmaceutics15010229

77.

Shi

Chen

, et al. Ginsenoside Rg5 activates the LKB1/AMPK/mTOR signaling pathway and modifies the gut Microbiota to alleviate nonalcoholic fatty liver disease induced by a high-fat diet. Nutrients. 2024;16(6):842. https://doi.org/10.3390/nu16060842

78.

Zhang

Fan

. Ginsenoside CK ameliorates hepatic lipid accumulation via activating the LKB1/AMPK pathway In Vitro and In Vivo. Food Funct. 2022;13(3):1153‐1167. https://doi.org/10.1039/d1fo03026d

79.

Liu

Zhang

Dai

, et al. A comprehensive review on the phytochemistry, pharmacokinetics, and antidiabetic effect of Ginseng. Phytomedicine. 2021;92(1):153717. https://doi.org/10.1016/j.phymed.2021.153717

80.

Huang

Wang

Yang

Wang

. Ginsenoside Rb2 alleviates hepatic lipid accumulation by restoring autophagy via induction of Sirt1 and activation of AMPK. Int. J. Mol. Sci. 2017;18(5):1063. https://doi.org/10.3390/ijms18051063

81.

Xiao

Zhang

Yang

, et al. Ginsenoside Rg1 ameliorates palmitic acid-induced hepatic steatosis and inflammation in HepG2 cells via the AMPK/NF-κB pathway. Int. J. Endocrinol. 2019;2019(1):7514802. https://doi.org/10.1155/2019/7514802

82.

Zheng

Wang

Cheng

, et al. Ginsenoside Rb1 reduces H2O2-induced HUVEC dysfunction by stimulating the sirtuin-1/AMP-activated protein kinase pathway. Mol. Med. Rep. 2020;22(1):247‐256. https://doi.org/10.3892/mmr.2020.11096

83.

Hernández-García

Granado-Serrano

Martín-Gari

Naudí

Serrano

. Efficacy of Panax ginseng supplementation on blood lipid profile. A meta-analysis and systematic review of clinical randomized trials. J. Ethnopharmacol. 2019;243(1):112090. https://doi.org/10.1016/j.jep.2019.112090

84.

Yue

Fan

, et al. Long-term and liver-selected ginsenoside C-K nanoparticles retard NAFLD progression by restoring lipid homeostasis. Biomaterials. 2023;301(1):122291. https://doi.org/10.1016/j.biomaterials.2023.122291

85.

Morshed

Akter

Karim

Iqbal

Kang

Yang

. Bioconversion, pharmacokinetics, and therapeutic mechanisms of ginsenoside compound K and its analogues for treating metabolic diseases. Curr. Issues Mol. Biol. 2024;46(3):2320‐2342. https://doi.org/10.3390/cimb46030148

86.

Sharma

Lee

. Ginsenoside compound K: insights into recent studies on pharmacokinetics and health-promoting activities. Biomolecules. 2020;10(7):1028. https://doi.org/10.3390/biom10071028

87.

Zhu

Guo

Liu

, et al. Salvianolic acid A attenuates non-alcoholic fatty liver disease by regulating the AMPK-IGFBP1 pathway. Chem. Biol. Interact. 2024;400(1):111162. https://doi.org/10.1016/j.cbi.2024.111162

88.

Chen

Xie

, et al. Przewaquinone A inhibits angiotensin II-induced endothelial diastolic dysfunction activation of AMPK. Phytomedicine. 2024;133(1):155885. https://doi.org/10.1016/j.phymed.2024.155885

89.

Fang

Little

. Atheroprotective effects and molecular targets of tanshinones derived from herbal medicine danshen. Med. Res. Rev. 2018;38(1):201‐228. https://doi.org/10.1002/med.21438

90.

Liu

Song

, et al. RRP Regulates autophagy through the AMPK pathway to alleviate the effect of cell senescence on atherosclerosis. Oxid. Med. Cell. Longev. 2023;2023(1):9645789. https://doi.org/10.1155/2023/9645789

91.

Wei

You

Ling

, et al. Regulation of antioxidant system, lipids and fatty acid β-oxidation contributes to the cardioprotective effect of sodium tanshinone IIA sulphonate in isoproterenol-induced myocardial infarction in rats. Atherosclerosis. 2013;230(1):148‐156. https://doi.org/10.1016/j.atherosclerosis.2013.07.005

92.

Liu

. Salvia miltiorrhiza burge (danshen): a golden herbal medicine in cardiovascular therapeutics. Acta Pharmacol. Sin. 2018;39(5):802‐824. https://doi.org/10.1038/aps.2017.193

93.

Tang

Chen

, et al. A green and highly efficient method to deliver hydrophilic polyphenols of Salvia miltiorrhiza and Carthamus tinctorius for enhanced anti-atherosclerotic effect via metal-phenolic network. Colloids Surf. B Biointerfaces. 2022;215(1):112511. https://doi.org/10.1016/j.colsurfb.2022.112511

94.

Chen

Pan

Zhu

, et al. Biomimetic metal-phenolic nanocarrier for co-delivery of multiple phytomedical bioactive components for anti-atherosclerotic therapy. Int. J. Pharm. 2025;671(1):125228. https://doi.org/10.1016/j.ijpharm.2025.125228

95.

Wang

Zhang

, et al. Artemisia scoparia extract attenuates non-alcoholic fatty liver disease in diet-induced obesity mice by enhancing hepatic insulin and AMPK signaling independently of FGF21 pathway. Metabolism. 2013;62(9):1239‐1249. https://doi.org/10.1016/j.metabol.2013.03.004

96.

Cao

Duan

Liu

. Artemisinin attenuated atherosclerosis in high-fat diet-fed ApoE-/- mice by promoting macrophage autophagy through the AMPK/mTOR/ULK1 pathway. J. Cardiovasc. Pharmacol. 2020;75(4):321‐332. https://doi.org/10.1097/FJC.0000000000000794

97.

Jiang

Liu

Cao

. Artemisinin alleviates atherosclerotic lesion by reducing macrophage inflammation via regulation of AMPK/NF-κB/NLRP3 inflammasomes pathway. J. Drug Target. 2020;28(4):70‐79. https://doi.org/10.1080/1061186X.2019.1616296

98.

Majidiani

Musavi

Momtazi-Borojeni

. New roles of artemisinins in atherosclerosis progression. Phytother. Res. 2025;39(4):1847‐1857. https://doi.org/10.1002/ptr.8483

99.

Xie

Wei

Guo

, et al. Ginkgolide C Attenuated Western Diet-Induced Non-Alcoholic Fatty Liver Disease via Increasing AMPK Activation. Inflammation. 2024;48(2):770-782. https://doi.org/10.1007/s10753-024-02086-3

100.

Hsieh

Yang

, et al. Ginkgo biloba extract attenuates oxLDL-induced endothelial dysfunction via an AMPK-dependent mechanism. J. Appl. Physiol. 2013;114(2):274‐285. https://doi.org/10.1152/japplphysiol.00367.2012

101.

Luo

Fang

Dan

. Crocin ameliorates hepatic steatosis through activation of AMPK signaling in db/db mice. Lipids Health Dis. 2019;18(1):11. https://doi.org/10.1186/s12944-018-0955-6

102.

Zhang

Fan

Liu

, et al. Safflor yellow A protects vascular endothelial cells from ox-LDL-mediated damage. J. Recept. Signal Transduct. Res. 2022;42(1):52‐59. https://doi.org/10.1080/10799893.2020.1843492

103.

Abedimanesh

Bathaie

Ostadrahimi

Asghari Jafarabadi

Taban Sadeghi

. The effect of crocetin supplementation on markers of atherogenic risk in patients with coronary artery disease: a pilot, randomized, double-blind, placebo-controlled clinical trial. Food Funct. 2019;10(11):7461‐7475. https://doi.org/10.1039/c9fo01166h

104.

Abedimanesh

Motlagh

Abedimanesh

Bathaie

Separham

Ostadrahimi

. Effects of crocin and saffron aqueous extract on gene expression of SIRT1, AMPK, LOX1, NF-κB, and MCP-1 in patients with coronary artery disease: a randomized placebo-controlled clinical trial. Phytother. Res. 2020;34(5):1114‐1122. https://doi.org/10.1002/ptr.6580

105.

Moeini Badi

Bathaie

Borazjani

, et al. The effect of crocetin (a saffron carotenoid) supplementation on antioxidant and inflammatory indexes and serum leptin concentration in patients with coronary artery disease. Food Funct. 2025;16(11):4604‐4614. https://doi.org/10.1039/d4fo03396e

106.

Zhu

Liu

Shen

Cai

. Combination of luteolin and lycopene effectively protect against the “two-hit” in NAFLD through Sirt1/AMPK signal pathway. Life Sci. 2020;256(1):117990. https://doi.org/10.1016/j.lfs.2020.117990

107.

Guo

Huang

Wang

, et al. Therapeutic application of natural products: NAD+ metabolism as potential target. Phytomedicine. 2023;114(1):154768. https://doi.org/10.1016/j.phymed.2023.154768

108.

Dong

Ren

, et al. Luteolin decreases atherosclerosis in LDL receptor-deficient mice via a mechanism including decreasing AMPK-SIRT1 signaling in macrophages. Exp. Ther. Med. 2018;16(3):2593‐2599. https://doi.org/10.3892/etm.2018.6499

109.

Luo

Shang

. Luteolin: a flavonoid that has multiple cardio-protective effects and its molecular mechanisms. Front. Pharmacol. 2017;8(1):692. https://doi.org/10.3389/fphar.2017.00692

110.

Chen

Liu

, et al. Scutellaria baicalensis regulates FFA metabolism to ameliorate NAFLD through the AMPK-mediated SREBP signaling pathway. J. Nat. Med. 2018;72(3):655‐666. https://doi.org/10.1007/s11418-018-1199-5

111.

Gao

Liu

Hao

, et al. Baicalin ameliorates high fat diet-induced nonalcoholic fatty liver disease in mice via adenosine monophosphate-activated protein kinase-mediated regulation of SREBP1/Nrf2/NF-κB signaling pathways. Phytother. Res. 2023;37(6):2405‐2418. https://doi.org/10.1002/ptr.7762

112.

Chen

, et al. Baicalin attenuates high fat diet-induced insulin resistance and ectopic fat storage in skeletal muscle, through modulating the protein kinase B/glycogen synthase kinase 3 Beta pathway. Chin. J. Nat. Med. 2016;14(1):48‐55. https://doi.org/10.3724/SP.J.1009.2016.00048

113.

Wang

Huang

Liang

, et al. Immuno-modulatory role of baicalin in atherosclerosis prevention and treatment: current scenario and future directions. Front. Immunol. 2024;15(1):1377470. https://doi.org/10.3389/fimmu.2024.1377470

114.

Gnoni

Di Chiara Stanca

Giannotti

Gnoni

Siculella

Damiano

. Quercetin reduces lipid accumulation in a cell model of NAFLD by inhibiting De Novo fatty acid synthesis through the acetyl-CoA carboxylase 1/AMPK/PP2A axis. Int. J. Mol. Sci. 2022;23(3):1044. https://doi.org/10.3390/ijms23031044

115.

Tsai

Chen

, et al. Regulatory effects of quercetin on M1/M2 macrophage polarization and oxidative/antioxidative balance. Nutrients. 2021;14(1):67. https://doi.org/10.3390/nu14010067

116.

Kim

Choi

. Quercetin-Induced AMP-activated protein kinase activation attenuates vasoconstriction through LKB1-AMPK signaling pathway. J. Med. Food. 2018;21(2):146‐153. https://doi.org/10.1089/jmf.2017.4052

117.

Cui

Wang

Qin

. Quercetin intervention reduced hepatic fat deposition in patients with nonalcoholic fatty liver disease: a randomized, double-blind, placebo-controlled crossover clinical trial. Am. J. Clin. Nutr. 2024;120(3):507‐517. https://doi.org/10.1016/j.ajcnut.2024.07.013

118.

Arabi

Shahraki Jazinaki

Chambari

, et al. The effects of quercetin supplementation on cardiometabolic outcomes: an umbrella review of meta-analyses of randomized controlled trials. Phytother. Res. 2023;37(11):5080‐5091. https://doi.org/10.1002/ptr.7971

119.

Atteia

AlFaris

Alshammari

, et al. The hepatic antisteatosis effect of Xanthohumol in high-fat diet-fed rats entails activation of AMPK as a possible protective mechanism. Foods. 2023;12(23):4214. https://doi.org/10.3390/foods12234214

120.

Doddapattar

Radović

Patankar

, et al. Xanthohumol ameliorates atherosclerotic plaque formation, hypercholesterolemia, and hepatic steatosis in ApoE-deficient mice. Mol. Nutr. Food Res. 2013;57(10):1718‐1728. https://doi.org/10.1002/mnfr.201200794

121.

Cheng

Feng

Sheng

Yang

. Nobiletin from Citrus peel: a promising therapeutic agent for liver disease-pharmacological characteristics, mechanisms, and potential applications. Front. Pharmacol. 2024;15(1):1354809. https://doi.org/10.3389/fphar.2024.1354809

122.

Tsuboi

Yonezawa

, et al. Molecular mechanism for nobiletin to enhance ABCA1/G1 expression in mouse macrophages. Atherosclerosis. 2020;297(1):32‐39. https://doi.org/10.1016/j.atherosclerosis.2020.01.024

123.

Santamarina

Oliveira

Silva

, et al. Green tea extract rich in epigallocatechin-3-gallate prevents fatty liver by AMPK activation via LKB1 in mice fed a high-fat diet. PLoS ONE. 2015;10(11):e0141227. https://doi.org/10.1371/journal.pone.0141227

124.

Zhang

Yin

Lang

Yang

Zhao

. Epigallocatechin-3-Gallate ameliorates hepatic damages by relieve FGF21 resistance and promotion of FGF21-AMPK pathway in mice fed a high fat diet. Diabetol. Metab. Syndr. 2022;14(1):53. https://doi.org/10.1186/s13098-022-00823-y

125.

Pai

Chou

Chan

, et al. Epigallocatechin gallate reduces homocysteine-caused oxidative damages through modulation SIRT1/AMPK pathway in endothelial cells. Am. J. Chin. Med. 2021;49(1):113‐129. https://doi.org/10.1142/S0192415X21500063

126.

Zhang

Wang

, et al. Preclinical evidence construction for epigallocatechin-3-gallate against non-alcoholic fatty liver disease: a meta-analysis and machine learning study. Phytomedicine. 2025;142(1):156651. https://doi.org/10.1016/j.phymed.2025.156651

127.

Eng

Thanikachalam

Ramamurthy

. Molecular understanding of epigallocatechin gallate (EGCG) in cardiovascular and metabolic diseases. J. Ethnopharmacol. 2018;210(1):296‐310. https://doi.org/10.1016/j.jep.2017.08.035

128.

Fang

Song

Zhou

, et al. Molecular mechanism pathways of natural compounds for the treatment of non-alcoholic fatty liver disease. Molecules. 2023;28(15):5645. https://doi.org/10.3390/molecules28155645

129.

Parsamanesh

Asghari

Sardari

, et al. Resveratrol and endothelial function: a literature review. Pharmacol. Res. 2021;170(1):105725. https://doi.org/10.1016/j.phrs.2021.105725

130.

Molaei

Sadeghnia

Hayes

Karimi

. LKB1: an emerging therapeutic target for cardiovascular diseases. Life Sci. 2022;306(1):120844. https://doi.org/10.1016/j.lfs.2022.120844

131.

Zhang

Chen

Zhou

, et al. Resveratrol improves hepatic steatosis by inducing autophagy through the cAMP signaling pathway. Mol. Nutr. Food Res. 2015;59(8):1443‐1457. https://doi.org/10.1002/mnfr.201500016

132.

Dong

Wang

, et al. Inhibitory effects of resveratrol on foam cell formation are mediated through monocyte chemotactic protein-1 and lipid metabolism-related proteins. Int. J. Mol. Med. 2014;33(5):1161‐1168. https://doi.org/10.3892/ijmm.2014.1680

133.

Vasamsetti

Karnewar

Gopoju

134.

Zordoky

Robertson

Dyck

. Preclinical and clinical evidence for the role of resveratrol in the treatment of cardiovascular diseases. Biochim. Biophys. Acta. 2015;1852(6):1155‐1177. https://doi.org/10.1016/j.bbadis.2014.10.016

135.

Chen

Jin

, et al. Resveratrol attenuates vascular endothelial inflammation by inducing autophagy through the cAMP signaling pathway. Autophagy. 2013;9(12):2033‐2045. https://doi.org/10.4161/auto.26336

136.

Jakubczyk

Skonieczna-Żydecka

Kałduńska

Stachowska

Gutowska

Janda

. Effects of resveratrol supplementation in patients with non-alcoholic fatty liver disease-A meta-analysis. Nutrients. 2020;12(8):2435. https://doi.org/10.3390/nu12082435

137.

Cho

Namkoong

Shin

, et al. Cardiovascular protective effects and clinical applications of resveratrol. J. Med. Food. 2017;20(4):323‐334. https://doi.org/10.1089/jmf.2016.3856

138.

Santana

Ogawa

Rogero

Barroso

Alves de Castro

. Effect of resveratrol supplementation on biomarkers associated with atherosclerosis in humans. Complement. Ther. Clin. Pract. 2022;46(1):101491. https://doi.org/10.1016/j.ctcp.2021.101491

139.

Zhang

Song

, et al. Gallic acid impairs fructose-driven De Novo lipogenesis and ameliorates hepatic steatosis via AMPK-dependent suppression of SREBP-1/ACC/FASN cascade. Eur. J. Pharmacol. 2023;940(1):175457. https://doi.org/10.1016/j.ejphar.2022.175457

140.

Tanaka

Sato

Kishimoto

Mabashi-Asazuma

Kondo

Iida

. Gallic acid inhibits lipid accumulation via AMPK pathway and suppresses apoptosis and macrophage-mediated inflammation in hepatocytes. Nutrients. 2020;12(5):1479. https://doi.org/10.3390/nu12051479

141.

Lin

Wang

. Gallic acid attenuates oleic acid-induced proliferation of vascular smooth muscle cell through regulation of AMPK-eNOS-FAS signaling. Curr. Med. Chem. 2013;20(31):3944‐3953. https://doi.org/10.2174/09298673113209990175

142.

Song

, et al. The role of gallic acid in liver disease: a review of its phytochemistry, pharmacology, and safety. Front. Pharmacol. 2025;16(1):1595508. https://doi.org/10.3389/fphar.2025.1595508

143.

Quan

Kim

Chung

. Betulinic acid alleviates non-alcoholic fatty liver by inhibiting SREBP1 activity via the AMPK-mTOR-SREBP signaling pathway. Biochem. Pharmacol. 2013;85(9):1330‐1340. https://doi.org/10.1016/j.bcp.2013.02.007

144.

Kim

Jung

Ryu

, et al. Betulinic acid inhibits high-fat diet-induced obesity and improves energy balance by activating AMPK. Nutr. Metab. Cardiovasc. Dis. 2019;29(4):409‐420. https://doi.org/10.1016/j.numecd.2018.12.001

145.

Jin

Choi

Hwang

, et al. Betulinic acid increases eNOS phosphorylation and NO synthesis via the calcium-signaling pathway. J. Agric. Food Chem. 2016;64(4):785‐791. https://doi.org/10.1021/acs.jafc.5b05416

146.

Jiang

Kong

. Gastrodin ameliorates oxidative stress and proinflammatory response in nonalcoholic fatty liver disease through the AMPK/Nrf2 pathway. Phytother. Res. 2016;30(3):402‐411. https://doi.org/10.1002/ptr.5541

147.

Wan

Zhang

Yang

, et al. Gastrodin improves nonalcoholic fatty liver disease through activation of the adenosine monophosphate-activated protein kinase signaling pathway. Hepatology. 2021;74(6):3074‐3090. https://doi.org/10.1002/hep.32068

148.

Tao

Yang

Xie

, et al. Gastrodin induces lysosomal biogenesis and autophagy to prevent the formation of foam cells via AMPK-FoxO1-TFEB signalling axis. J. Cell. Mol. Med. 2021;25(12):5769‐5781. https://doi.org/10.1111/jcmm.16600

149.

Huang

Zhou

. Hepatic AMP kinase as a potential target for treating nonalcoholic fatty liver disease: evidence from studies of natural products. Curr. Med. Chem. 2018;25(8):889‐907. https://doi.org/10.2174/0929867324666170404142450

150.

García-Muñoz

Victoria-Montesinos

Ballester

Cerdá

Zafrilla

. A descriptive review of the antioxidant effects and mechanisms of action of berberine and silymarin. Molecules. 2024;29(19):4576. https://doi.org/10.3390/molecules29194576

151.

Zhu

Bian

Wang

, et al. Berberine attenuates nonalcoholic hepatic steatosis through the AMPK-SREBP-1c-SCD1 pathway. Free Radic. Biol. Med. 2019;141(1):192‐204. https://doi.org/10.1016/j.freeradbiomed.2019.06.019

152.

Fatahian

Haftcheshmeh

Azhdari

Farshchi

Nikfar

Momtazi-Borojeni

. Promising anti-atherosclerotic effect of berberine: evidence from in vitro, in vivo, and clinical studies. Rev. Physiol. Biochem. Pharmacol. 2020;178(1):83‐110. https://doi.org/10.1007/112_2020_42

153.

Peng

, et al. Berberine: a review of its pharmacokinetics properties and therapeutic potentials in diverse vascular diseases. Front. Pharmacol. 2021;12(1):762654. https://doi.org/10.3389/fphar.2021.762654

154.

Ren

Wang

, et al. Preclinical evidence of berberine on non-alcoholic fatty liver disease: a systematic review and meta-analysis of animal studies. Front. Pharmacol. 2021;12(1):742465. https://doi.org/10.3389/fphar.2021.742465

155.

Chen

Liu

Deng

. Protective effects of berberine on nonalcoholic fatty liver disease in db/db mice via AMPK/SIRT1 pathway activation. Curr. Med. Sci. 2024;44(5):902‐911. https://doi.org/10.1007/s11596-024-2914-y

156.

Koperska

Wesołek

Moszak

Szulińska

. Berberine in non-alcoholic fatty liver disease-A review. Nutrients. 2022;14(17):3459. https://doi.org/10.3390/nu14173459

157.

Nie

Huang

, et al. The clinical efficacy and safety of berberine in the treatment of non-alcoholic fatty liver disease: a meta-analysis and systematic review. J. Transl. Med. 2024;22(1):225. https://doi.org/10.1186/s12967-024-05011-2

158.

Song

Hao

Fan

. Biological properties and clinical applications of berberine. Front. Med. 2020;14(5):564‐582. https://doi.org/10.1007/s11684-019-0724-6

159.

Fatahian

Haftcheshmeh

Azhdari

Farshchi

Nikfar

Momtazi-Borojeni

. Promising anti-atherosclerotic effect of berberine: evidence from In Vitro, In Vivo, and clinical studies. Rev. Physiol. Biochem. Pharmacol. 2020;178(1):83‐110. https://doi.org/10.1007/112_2020_42

160.

Zheng

Yang

, et al. Salidroside attenuates high-fat diet-induced nonalcoholic fatty liver disease via AMPK-dependent TXNIP/NLRP3 pathway. Oxid. Med. Cell. Longev. 2018;2018(1):8597897. https://doi.org/10.1155/2018/8597897

161.

Zhang

, et al. Salidroside activates the AMP-activated protein kinase pathway to suppress nonalcoholic steatohepatitis in mice. Hepatology. 2021;74(6):3056‐3073. https://doi.org/10.1002/hep.32066

162.

Xing

Yang

Zheng

, et al. Salidroside improves endothelial function and alleviates atherosclerosis by activating a mitochondria-related AMPK/PI3 K/akt/eNOS pathway. Vasc. Pharmacol. 2015;72(1):141‐152. https://doi.org/10.1016/j.vph.2015.07.004

163.

Lan

Zhang

, et al. Cordycepin ameliorates nonalcoholic steatohepatitis by activation of the AMP-activated protein kinase signaling pathway. Hepatology. 2021;74(2):686‐703. https://doi.org/10.1002/hep.31749

164.

Zhou

Zhang

Cao

Guo

. Cordycepin stimulates autophagy in macrophages and prevents atherosclerotic plaque formation in ApoE-/- mice. Oncotarget. 2017;8(55):94726‐94737. https://doi.org/10.18632/oncotarget.21886

165.

Liu

Wang

, et al. 6-Gingerol Ameliorates hepatic steatosis, inflammation and oxidative stress in high-fat diet-fed mice through activating LKB1/AMPK signaling. Int. J. Mol. Sci. 2023;24(7):6285. https://doi.org/10.3390/ijms24076285

166.

Alipour

Baradaran Rahimi

Askari

. Promising influences of gingerols against metabolic syndrome: a mechanistic review. Biofactors. 2022;48(5):993‐1004. https://doi.org/10.1002/biof.1892

167.

Wang

Tian

Yang

, et al. 6-Gingerol Ameliorates behavioral changes and atherosclerotic lesions in ApoE-/- mice exposed to chronic mild stress. Cardiovasc. Toxicol. 2018;18(5):420‐430. https://doi.org/10.1007/s12012-018-9452-4

168.

Wang

Liu

Chen

, et al. Pharmacokinetics of ginsenoside Rh2, the Major anticancer ingredient of ginsenoside H dripping pills, in healthy subjects. Clin. Pharmacol. Drug Dev. 2021;10(6):669‐674. https://doi.org/10.1002/cpdd.877

169.

Wang

Zheng

Yang

. Pharmacokinetic and metabolism study of ginsenoside Rb2 in rat by liquid chromatography combined with electrospray ionization tandem mass spectrometry. Biomed. Chromatogr. 2021;35(11):e5191. https://doi.org/10.1002/bmc.5191

170.

Vijayakumar

Baskaran

Maeng

Yoo

. Ginsenoside improves physicochemical properties and bioavailability of curcumin-loaded nanostructured lipid carrier. Arch. Pharm. Res. 2017;40(7):864‐874. https://doi.org/10.1007/s12272-017-0930-1

171.

Vijayakumar

Baskaran

Baek

Sundaramoorthy

Yoo

. In Vitro cytotoxicity and bioavailability of ginsenoside-modified nanostructured lipid carrier containing curcumin. AAPS PharmSciTech. 2019;20(2):88. https://doi.org/10.1208/s12249-019-1295-1

172.

Zare-Zardini

Alemi

Taheri-Kafrani

, et al. Assessment of a new ginsenoside Rh2 nanoniosomal formulation for enhanced antitumor efficacy on prostate cancer: an in vitro study. Drug Des. Devel. Ther. 2020;14(1):3315‐3324. https://doi.org/10.2147/DDDT.S261027

173.

Kim

, et al. Pharmacokinetics of ginsenoside Rb1, Rg3, Rk1, Rg5, F2, and compound K from red Ginseng extract in healthy Korean volunteers. Evid. Based Complement. Alternat. Med. 2022;2022(1):8427519. https://doi.org/10.1155/2022/8427519

174.

Zhang

, et al. Research on Q-markers of qiliqiangxin capsule for chronic heart failure treatment based on pharmacokinetics and pharmacodynamics association. Phytomedicine. 2018;44(1):220‐230. https://doi.org/10.1016/j.phymed.2018.03.003

175.

Feng

Fang

Zhang

. The melanin inhibitory effect of plants and phytochemicals: a systematic review. Phytomedicine. 2022;107(1):154449. https://doi.org/10.1016/j.phymed.2022.154449

176.

Shao

Sun

, et al. Effects of borneol on the release of compound danshen colon-specific osmotic pump capsule In Vitro and pharmacokinetics study in beagle dogs. AAPS PharmSciTech. 2020;21(8):316. https://doi.org/10.1208/s12249-020-01840-8

177.

Yoo

Park

Kim

, et al. Ginsenoside absorption rate and extent enhancement of black Ginseng (CJ EnerG) over red Ginseng in healthy adults. Pharmaceutics. 2021;13(4):487. https://doi.org/10.3390/pharmaceutics13040487

178.

Liang

Zhang

Hou

Feng

Yin

. Pharmacokinetics study of ginsenoside Rg1 liposome by pulmonary administration. Heliyon. 2024;10(9):e29906. https://doi.org/10.1016/j.heliyon.2024.e29906

179.

Zheng

Chu

, et al. Enhancing effect of borneol on pharmacokinetics of ginsenoside Rb1, ginsenoside Rg1, and notoginsenoside R1 in healthy volunteers after oral administration of compound danshen dropping pills. Biomed. Chromatogr. 2022;36(5):e5311. https://doi.org/10.1002/bmc.5311

180.

Jeon

Lee

Choi

Song

. Pharmacokinetics of ginsenosides following repeated oral administration of red ginseng extract significantly differ between species of experimental animals. Arch. Pharm. Res. 2020;43(12):1335‐1346. https://doi.org/10.1007/s12272-020-01289-0

181.

Kim

Choi

Park

Kee

Kim

Yoo

. Pharmacokinetic profiling of ginsenosides, Rb1, rd, and Rg3, in mice with antibiotic-induced gut Microbiota alterations: implications for variability in the therapeutic efficacy of red Ginseng extracts. Foods. 2023;12(23):4342. https://doi.org/10.3390/foods12234342

182.

Zhang

Zhu

Song

, et al. Ginsenoside Rb1, compound K and 20(S)-protopanaxadiol attenuate high-fat diet-induced hyperlipidemia in rats via modulation of gut Microbiota and bile acid metabolism. Molecules. 2024;29(5):1108. https://doi.org/10.3390/molecules29051108

183.

Morshed

Akter

Karim

Iqbal

Kang

Yang

184.

Zheng

Chen

, et al. Influence of B-Complex vitamins on the pharmacokinetics of ginsenosides Rg1, Rb1, and ro after oral administration. J. Med. Food. 2017;20(11):1127‐1132. https://doi.org/10.1089/jmf.2017.3922

185.

Olaleye

Niu

, et al. Multiple circulating saponins from intravenous ShenMai inhibit OATP1Bs in vitro: potential joint precipitants of drug interactions. Acta Pharmacol. Sin. 2019;40(6):833‐849. https://doi.org/10.1038/s41401-018-0173-9

186.

Zhou

, et al. Herb-drug interaction: a case study of effects and involved mechanisms of cisplatin on the pharmacokinetics of ginsenoside Rb1 in tumor-bearing mice. Biomed. Pharmacother. 2019;110(1):95‐104. https://doi.org/10.1016/j.biopha.2018.11.021

187.

Chen

Wei

Jiang

, et al. Comparative pharmacokinetics of seven bioactive components after oral administration of crude and processed qixue shuangbu prescription in chronic heart failure rats by microdialysis combined with UPLC-MS/MS. J. Ethnopharmacol. 2023;303(1):116035. https://doi.org/10.1016/j.jep.2022.116035

188.

Zhang

Geng

, et al. High-throughput metabolomics and ingenuity pathway approach reveals the pharmacological effect and targets of ginsenoside Rg1 in Alzheimer's disease mice. Sci. Rep. 2019;9(1):7040. https://doi.org/10.1038/s41598-019-43537-4

189.

Hou

Peng

, et al. Ginsenoside Rg1 regulates liver lipid factor metabolism in NAFLD model rats. ACS Omega. 2020;5(19):10878‐10890. https://doi.org/10.1021/acsomega.0c00529

190.

Cheng

Gao

, et al. Ginsenoside Rg2 ameliorates high-fat diet-induced metabolic disease through SIRT1. J. Agric. Food Chem. 2020;68(14):4215‐4226. https://doi.org/10.1021/acs.jafc.0c00833

191.

Zhang

Feng

Jiang

Wang

. Ginsenoside compound K-based multifunctional liposomes for the treatment of rheumatoid arthritis. Drug Deliv. 2025;32(1):2464190. https://doi.org/10.1080/10717544.2025.2464190

192.

Liu

Jiang

, et al. Ginsenoside Rg3 ameliorates myocardial glucose metabolism and insulin resistance via activating the AMPK signaling pathway. J. Ginseng Res. 2022;46(2):235‐247. https://doi.org/10.1016/j.jgr.2021.06.001

193.

Kadoglou

NPE

Christodoulou

Kostomitsopoulos

Valsami

. The cardiovascular-protective properties of saffron and its potential pharmaceutical applications: a critical appraisal of the literature. Phytother. Res. 2021;35(12):6735‐6753. https://doi.org/10.1002/ptr.7260

Natural Products Ameliorate Non-Alcoholic Fatty Liver Disease and Atherosclerosis via the LKB1/AMPK Pathway

Abstract

Keywords

Introduction

Method

Literature Search and Selection Criteria for Natural Products

Inclusion and Exclusion Criteria

Data Extraction and Quality Assessment

Pathological Mechanisms Linking NAFLD and AS

Association Between NAFLD and AS

Epidemiological and Biomarker Evidence

Key Mechanisms

Biological Functions of the LKB1/AMPK Signaling Pathway

Basic Overview of the LKB1/AMPK Pathway

Role of LKB1/AMPK in Metabolic Regulation:

Lipid Metabolism

Inflammatory Response

Endothelial Function

Mitochondrial Function

Energy Metabolism

Therapeutic Potential of Natural Products Targeting LKB1/AMPK Pathway in NAFLD and AS

Multi-Target Effects of Single Herbal Medicines

Rhizome-Derived Herbs

Leaf-Derived Herbs

Flower-Derived Herbs

Targeted Regulation of Monomeric Compounds

Polyphenolic Compound

Terpenoid Compound

Alkaloid Compound

Glycoside Compound

Nucleoside Compound

Phenolic Derivative

Pharmacological and Translational Challenges of Natural Products Targeting the LKB1/AMPK Pathway

Bioavailability Limitations

Pharmacokinetics and Metabolism

Species and Disease-State Variability

Translation Gaps from Animal to Human Studies

Future Perspectives

Discussion

LKB1/AMPK Signaling Hub: A Tripartite Regulatory Core Integrating Metabolism, Inflammation, and Mitochondria

Liver–Vascular Axis Crosstalk: Exosomal miRNA-Mediated Communication

Multi-Target Intervention by Natural Products: From Molecular Synergy to Clinical Translation Challenges

Conclusion and Limitations

Footnotes

Abbreviations

ORCID iD

Ethical Approval

Author Contributions

Funding

Declaration of Conflicting Interests

Data Availability Statement

Statement of Human and Animal Rights

Statement of Informed Consent

References