Sea urchin pigments, also known as spinochromes or polyhydroxynaphthoquinones (PHNQ) have been well studied for their bioactive properties like anti-bacterial, fungicidal, antioxidant and pro-inflammatory effects. Moreover, many studies have analyzed and identified these pigments in sea urchin tests and spines but, and to the best of our knowledge, never in their body compartments or in a quantitative manner. The aim of this work was to investigate the diversity and concentration of pigments in three sea urchins localized in the same coral reef in Madagascar (Diadema savignyi, Tripneustes gratilla and Toxopneustes pileolus). Their pigment distribution patterns were compared and linked to their behavior in order to understand better the implication of spinochromes in a sea urchin's life. Analyses and quantifications by LC-MS showed the presence of 11 different spinochromes with principally two kinds of patterns. First, D. savignyi showed a high concentration in all its body compartments with a predominance for Echinochrome A and Spinochrome D – Iso 3, a pattern close to the E. mathaei distribution found in our previous study. The presence of these pigments has been linked to their bioactive properties and suggested their implications in UV radiation protection, in reproduction, in feeding as well as in their immune systems. Secondly, T. gratilla and T. pileolus presented few spinochromes, which could explain their restricted diet and their covering behavior facing sunlight. Moreover, the absence of pigments in their gonads could be explained by their seasonal spawning occurring before our collection period. Nevertheless, the near absence of spinochromes may be counterbalanced by other bioactive compounds like their toxic proteins, which may act as either a defense mechanism or immune agents, or like carotenoid pigments already shown in sea urchin and acting as defense agents. In conclusion, spinochromes, and pigments in general, seem to play many roles in sea urchin ecology. Their highly reactive structures confer them with strong bioactive properties involved in most parts of their metabolic system and clearly participate in the survival of the sea urchins.
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