Asparagus racemosus Willd. or Shatavari (Asparagaceae family) is an important medicinal plant in Ayurvedic medicine as a rejuvenate for women. A method for quantitative analysis of saponin glycosides bioactive constituents in A. racemosus is reported. A high performance liquid chromatography quadrupole time of flight mass spectrometry (HPLC-Q-TOF-MS/MS) method was developed and validated for simultaneous determination of five saponin glycosides, asparacoside, shatavarin IX, shatavarin IV, asparanin A and shatavarin V in A. racemosus extracted with 70% MeOH. The method was validated through intra-and inter-day precision, with the relative standard deviation (RSD) less than 6%, limits of detection (LOD) and limits of quantification (LOQ) <10 and 50 ng, respectively. Overall recoveries ranged from 95% to 105%, with RSD ranging from 0.7% to 4.5%. The method was applied to saponin glycoside contents in the leaves, stems, and roots of A. racemosus sourced from different geographical locations, including four provinces in Thailand, and a sample from India. Saponin glycosides were detected predominantly in the roots, the part used in traditional medicines and these showed wide variations in saponin glycoside profiles from undetectable to 12 mg/g dry weight. The quality control of A. racemosus is crucial for reliable and predictable therapies and only methods like the one developed has the necessary flexibility, sensitivity, accuracy, and selectivity for reliable routine quality control.
BopanaN, SaxenaS. (2007) Asparagus racemosus-Ethnopharmacological evaluation and conservation needs. Journal of Ethnopharmacology, 110, 1–15.
2.
GoelR, SairamK. (2002) Anti-ulcer drugs from indigenous sources with emphasis on Musa sapientum, Tamrahbasma, Asparagus racemosus and Zingiber officinale. Indian Journal of Pharmacology, 34, 100–110;
3.
SairamK, PriyambadaS, AryyaN, GoelR. (2003) Gastroduodenal ulcer protective activity of Asparagus racemosus: an experimental, biochemical and histological study. Journal of Ethnopharmacology, 86, 1–10.
4.
SharmaS; RamjiS; KumariS, BapnaJ. (1996) Randomized controlled trial of Asparagus racetnosus (shatavari) as a lactogogue in lactational inadequacy. Indian Pediatrics, 33, 675–677.
5.
PandeySK, SahayA, PandeyRS, TripathiYB. (2005) Effect of Asparagus racemosus rhizome (Shatavari) on mammary gland and genital organs of pregnant rat. Phytotherapy Research, 19, 721–724.
6.
OnlomC, KhanthawongS, WaranuchN, IngkaninanK. (2014) In vitro anti-malassezia activity and potential use in anti-dandruff formulation of Asparagus racemosus. International Journal of Cosmetic Science, 36, 74–78.
7.
VenkatesanN, ThiyagarajanV, NarayananS, ArulA, RajaS, VijayaKumar SG, RajarajanT, PerianayagamJB. (2005) Anti-diarrhoeal potential of Asparagus racemosus wild root extracts in laboratory animals. Journal of Pharmacy and Pharmaceutical Sciences, 8, 39–46.
8.
BhatnagarM, SisodiaSS, BhatnagarR. (2005) Antiulcer and antioxidant activity of Asparagus racemosus Willd. and Withania somnifera Dunal. in rats. Annals of the New York Academy of Sciences, 1056, 261–278.
9.
KongkaneramitL, WitoonsaridsilpW, PeungvichaP, IngkaninanK, WaranuchN, SarisutaN. (2011) Antioxidant activity and antiapoptotic effect of Asparagus racemosus root extracts in human lung epithelial H460 cells. Experimental and Therapeutic Medicine, 2, 143–148.
10.
BhutaniK, PaulA, FayadW, LinderS. (2010) Apoptosis inducing activity of steroidal constituents from Solanum xanthocarpum and Asparagus racemosus. Phytomedicine, 17, 789–793.
11.
LiuW, HuangXF, QiQ, DaiQS, YangL, NieFF, LuN, GongDD, KongLY, GuoQL. (2009) Asparanin A induces G 2/M cell cycle arrest and apoptosis in human hepatocellular carcinoma HepG2 cells. Biochemical and Biophysical Research Communications, 381, 700–705.
12.
HayesPY, JahidinAH, LehmannR, PenmanK, KitchingW, De VossJJ. (2008) Steroidal saponins from the roots of Asparagus racemosus. Phytochemistry, 69, 796–804.
13.
HayesPY, JahidinAH, LehmannR, PenmanK, KitchingW, De VossJJ. (2006) Structural revision of shatavarins I and IV, the major components from the roots of Asparagus racemosus. Tetrahedron letters, 47, 6965–6969.
14.
HayesPY, JahidinAH, LehmannR, PenmanK, KitchingW, De VossJJ. (2006) Asparinins, asparosides, curillins, curillosides and shavatarins: structural clarification with the isolation of shatavarin V, a new steroidal saponin from the root of Asparagus racemosus. Tetrahedron Letters, 47, 8683–8687.
15.
HandaSS, SuriOP, GuptaVN, SuriKA, SattiNK, BhardwajV, BediKL, KhajuriaA, KaulA, ParikhGG. (2003) Process for the isolation of novel oligospirostanoside. US Patents; US6670459, 2003.
16.
SharmaU, KumarN, SinghB. (2012) Furostanol saponin and diphenylpentendiol from the roots of Asparagus racemosus. Natural Product Communications, 7, 995–998.
17.
MitraSK, PrakashNS, SundaramR. (2012) Shatavarins (containing Shatavarin IV) with anticancer activity from the roots of Asparagus racemosus. Indian Journal of Pharmacology, 44, 732–736.
18.
DuttaA, GhoshalA, MandalD, MondalNB, BanerjeeS, SahuNP, MandalC (2007) Racemoside A, an anti-leishmanial, water-soluble, natural steroidal saponin, induces programmed cell death in Leishmania donovani. Journal of Medical Microbiology, 56, 1196–1204.
19.
OnlomC, YangY, AisaHA, WaranuchN, PhrompittayaratW, PutaluneW, IngkaninanK. (2017) Preparative and rapid purification of saponins from Asparagus racemosus root by high performance centrifugal partition chromatography, Natural Product Communications, 12, in press.
20.
SattiN, SuriK, DuttP, SuriO, AminaM, QaziG, RaufA. (2006) Evaluation of Asparagus racemosus on the basis of immunomodulating sarsasapogenin glycosides by HPTLC. Journal of Liquid Chromatography and Related Technologies, 29, 219–227.
21.
AsmariS, ZafarR, AhmadS. (2010) Production of sarsasapogenin from tissue culture of Asparagus racemosus and its quantification by HPTLC. Iranian Journal of Pharmaceutical Research, 3, 66–67.
22.
NegiJS, SinghP, nee PantGJ, RawatM. (2011) High performance liquid chromatographic analysis of derivatized sapogenin of Asparagus (RP-HPLC analysis of derivatized sapogenin of Asparagus). Journal of Medicinal Plants Research, 5, 1900–1904.
23.
ReamyindeeN. (2011) The Immunological Methods for Detection of Saponins in Asparagus racemosus Roots. MS thesis, Naresuan University.
24.
HuberL. (1998) Validation of analytical methods. Validation and Qualification in the Analytical Laboratories, Interpharm Press, Buffalo Grove, IL, 107.
25.
RiveraC, RodríguezR. (2014) Horwitz equation as quality benchmark in ISO/IEC 17025 testing laboratory. Private communication.
26.
BoonsomT, WaranuchN, IngkaninanK, DenduangboripantJ, SukrongS. (2012) Molecular analysis of the genus Asparagus based on matK sequences and its application to identify A. racemosus, a medicinally phytoestrogenic species. Fitoterapia, 83, 947–953.
27.
VijayN, SairkarP, SilawatN, GargR, MehrotraN. (2009) Genetic variability in Asparagus racemosus (Willd.) from Madhya Pradesh, India by random amplified polymorphic DNA. African Journal of Biotechnology, 8, 3135–3140.
28.
LeeMK, ParkH; LeeCH. (1987) Effect of growth conditions on saponin content and ginsenoside pattern of Panax ginseng. In Korea-Japan Panax Ginseng Symposium, 89–107.
29.
MimakiY, YokosukaA, KurodaM, SashidaY. (2001) Cytotoxic activities and structure-cytotoxic relationships of steroidal saponins. Biological and Pharmaceutical Bulletin, 24, 1286–1289.
30.
ManS, GaoW, ZhangY, HuangL, LiuC. (2010) Chemical study and medical application of saponins as anti-cancer agents. Fitoterapia, 81, 703–714.
