For the first time, the bioactive compound tetramethylpyrazine (TMP) was isolated and identified from the culture of Pleurotus geesteranus Singer. Its identification was confirmed unambiguously by identical retention time and mass spectrum to those of a TMP standard. The TMP content in P. geesteranus from solid culture was up to 8.04 g.kg−1ds. This finding provides a novel natural way to produce TMP and will lead to new value added mushroom products with nutraceutical functions.
TanZ. (2009) Neural protection by naturopathic compounds—an example of tetramethylpyrazine from retina to brain. Journal of Ocular Biology, Diseases, and Information, 2, 137–144.
2.
KosugeT., AdachiT., KamiyaH. (1962) Isolation of tetramethylpyrazine from culture of Bacillus natto, and biosynthetic pathway of tetramethylpyrazine. Nature, 195, 1103–1103.
3.
OjewoleJ.A.O., OdebiyiO.O. (1981) Mechanism of the hypotensive effect of tetramethylpyrazine, an amide alkaloid from the stem of Jatropha podagrica. Journal of Medicinal Plant Research, 41, 281–287.
4.
KosugeT., ZendaH., TsujiK., YamamotoT., NaritaH. (1971) Studies on flavor components of foodstuffs Part I, Distribution of tetramethylpyrazine in fermented foodstuffs. Agricultural & Biological Chemistry, 35, 693–696.
