Proteases from the tentacles of the sea cucumber Isostichopus fuscus were partially purified and used to produce antioxidant peptides from body wall proteins of this marine species. Three proteins (105, 68, and 39 kDa) were identified by SDS-PAGE in the proteolytic extract of the tentacles. Protein hydrolyzates were generated with gelatin and crude protein substrates from body wall, and peptidic fractions lower and higher than 3 kDa were obtained to evaluate their oxygen radical scavenging capacity (ORAC). The 3 kDa-fraction obtained from the crude protein hydrolyzate showed the highest ORAC value (0.92 ± 0.04 μmol Trolox equivalent/mg protein). This fraction was selected to purify peptides potentially responsible for the activity that might be used as ingredients for development of functional foods.
SarmadiaBH, IsmailaA. (2010) Antioxidative peptides from food proteins: A review. Peptides, 31, 1949–1956.
2.
OwuorED, KongANT. (2002) Antioxidants and oxidants regulated signal transduction pathways. Biochemical Pharmacology, 64, 765–770.
3.
AmesBN, ShigenagaMK, HagenTM. (1993) Oxidants, antioxidants, and the degenerative diseases of aging. Proceedings of the National Academy of Sciences, 90, 7915–7922.
4.
ChenCH, PearsonAM, GrayJI. (1992) Effects of synthetic antioxidants (BHA, BHT and PG) on the mutagenicity of IG-like compounds. Food Chemistry, 43, 177–183.
5.
FreitasAC, AndradeJC, SilvaFM, Rocha-SantosTAP, DuarteAC, GomesAM. (2013) Antioxidative peptides: trends and perspectives for future research. Current Medicinal Chemistry, 20, 4575–4594.
6.
ChakrabartiS, JahandidehF, WuJ. (2014) Food-derived bioactive peptides on inflammation and oxidative stress. BioMed Research International Article, ID 608979.
7.
Food and Agriculture Organization of the United Nations. (2004) Advances in sea cucumber aquaculture and management. FAO Fisheries Technical Paper, 463, 347–358.
8.
Pérez-VegaJA, Olivera-CastilloL, Gómez-RuizJA, Hernández-LedesmaB. (2013) Release of multifunctional peptides by gastrointestinal digestion of sea cucumber (Isostichopus badionotus). Journal of Functional Foods, 5, 869–877.
9.
WuHL, HuYQ, ShenJD, CaiQF, LiuGM, SuWJ, CaoMJ. (2013) Identification of a novel gelatinolytic metalloproteinase (GMP) in the body wall of sea cucumber (Stichopus japonicus) and its involvement in collagen degradation. Process Biochemistry, 48, 871–877.
10.
QiH, DongXP, CongLN, GaoY, LiuL, MikiroT, ZhuBW. (2007) Purification and characterization of a cysteine-like protease from the body wall of the sea cucumber Stichopus japonicus. Fish Physiology and Biochemistry, 33, 181–188.
11.
SunLM, ZhuBW, WuHT, YuL, ZhouDY, DongX, YangJF, LiDM, YeWX, MurataY. (2011) Purification and characterization of cathepsin B from the gut of the sea cucumber (Stichopus japonicus). Food Science and Biotechnology, 20, 919–925.
12.
ZhuBW, ZhaoL, SunL, LiD, MurataY, YuL, ZhangL. (2008) Purification and characterization of a cathepsin L-like enzyme from the body wall of the sea cucumber Stichopus japonicus. Bioscience, Biotechnology, and Biochemistry, 72, 1430–1437.
13.
WuHT, LiDM, ZhuBW, SunJJ, ZhengJ, WangFL, KonnoK, JiangX. (2013) Proteolysis of noncollagenous proteins in sea cucumber, Stichopus japonicus, body wall: Characterization and the effects of cysteine protease inhibitors. Food Chemistry, 141, 1287–1294.
14.
Hernández-SámanoAC, Guzmán-GarcíaX, García-BarrientosR, Ascencio-ValleF, Sierra-BeltránA, Vallejo-CórdobaB, González-CórdovaAF, Torres-LlanezMJ, Guerrero-LegarretaI. (2015) Extraction and characterization of sea cucumber Isostichopus fuscus proteases, collected at the Gulf of California, Mexico. Revista Mexicana de Ingeniería Química, 14, 35–47.
15.
FuX, XueC, MiaoB, LiZ, GaoX, YangW. (2005) Characterization of proteases from the digestive tract of sea cucumber (Stichopus japonicus): High alkaline protease activity. Aquaculture, 246, 321–329.
16.
ZhouX, WangC, JiangA. (2012) Antioxidant peptides isolated from sea cucumber Stichopus japonicus. European Food Research and Technology, 234, 441–447.
17.
FuSJ, JinHZ. (2013) The relationship between antioxidant activity of sea cucumber peptide and its molecular weight. Food Science and Technology, 38, 63–66.
18.
ZhengJ, WuHT, ZhuBW, DongXP, ZhangMM, LiYL. (2012) Identification of antioxidative oligopeptides derived from autolysis hydrolysates of sea cucumber (Stichopus japonicus) guts. European Food Research and Technology, 234, 895–904.
19.
OsamaYA, RidzwanBH, MuhammadT, JamaludinMD, Masa-AkiI, ZaliBI. (2009) In vitro antioxidant and antiproliferative activities of three Malaysian sea cucumber species. European Journal of Scientific Research, 3, 376–387.
20.
KimSK, ByunHG, LeeEH. (1994) Optimum extraction conditions of gelatin from fish skins and its physical properties. Korean Society of Industrial and Engineering Chemistry, 5, 547–559.
21.
LaemmliUK. (1970) Cleavage of structure proteins during the assembly of head of bacteriophage T4. Nature, 277, 680–685.
22.
Hernández-LedesmaB, DávalosA, BartoloméB, AmigoL. (2005) Preparation of antioxidant enzymatic hydrolysates from alpha-lactalbumin and beta-lactoglobulin. Identification of active peptides by HPLC-MS/MS. Journal of Agricultural and Food Chemistry, 53, 588–593.
