The rhizomes and roots of Valeriana fauriei were extracted with 80% aqueous ethanol. This extract was found to exhibit potent inhibitory effects on fat accumulation in 3T3-L1 murine adipocytes. After several steps of chromatographic purification, we succeeded in identifying monovalerianester A as an inhibitor of fat accumulation. Thus, monovalerianester A and the crude extract of the rhizomes and roots of V. fauriei may have therapeutic potential for the treatment of obesity.
MannCC. (2005) Provocative study says obesity may reduce U.S. life expectancy. Science, 307, 1716–1717.
2.
ShimokawaK, MashimaI, AsaiA, YamadaK, KitaM, UemuraD. (2006) (-)-Ternatin, a highly N-methylated cyclic heptapeptide that inhibits fat accumulation: Structure and synthesis. Tetrahedron Letters, 47, 4445–4448.
3.
ShimokawaK, YamadaK, KitaM, UemuraD. (2007) Convergent synthesis and in vivo inhibitory effect on fat accumulation of (-)-ternatin, a highly N-methylated cyclic peptide. Bioorganic and Medicinal Chemistry Letters, 17, 4447–4449.
SashidaY, YamasakiK. (1997) Syouyakugaku (in Japanese). Nankodo, Tokyo, 258–260
6.
KonoshimaM, SawadaT, HataK. (1978) Syouyakugaku (in Japanese). Asakura Publishing, Tokyo, 234–236.
7.
GuoY, XuJ, LiY, WatanabeR, OshimaY, YamakuniT, OhizumiY. (2006) Iridoids and sesquiterpenoids with NGF-potentiating activity from the rhizomes and roots of Valeriana fauriei. Chemical and Pharmaceutical Bulletin, 54, 123–125.
8.
YukiK, IkedaM, MiyamotoK, OhnoO, YamadaK, UemuraD. (2012) Isolation of 9-hydroxy-10E,12Z-octadecadienoic acid, an inhibitor of fat accumulation from Valeriana fauriei. Bioscience, Biotechnology, and Biochemistry, 76, 1233–1235.
9.
ZhouY, FangY, GongZ, DuanX, LiuY. (2009) Two new terpenoids from Valeriana officinalis. Chinese Journal of Natural Medicines, 7, 270–273.
10.
ShimokawaK, IwaseY, MiwaR, YamadaK, UemuraD. (2008) Whole structure-activity relationships of the fat-accumulation inhibitor (-)-Ternatin: Recognition of the importance of each amino acid residue. Journal of Medicinal Chemistry, 51, 5912–5914.
11.
EndoT, TaguchiH. (1977) The constituents of valerian root. The structures of four new iridoid glycosides, Kanokoside A, B, C, and D from the root of “Hokkaikisso”. Chemical and Pharmaceutical Bulletin, 25, 2140–2142.
12.
GreenH, KehindeO. (1976) Spontaneous heritable changes leading to increased adipose conversion in 3T3 cells. Cell, 7, 105–113.
13.
SekiyaK, OkudaH, HottaY, ArichiS. (1987) Enhancement of adipose differentiation of mouse 3T3-L1 fibroblasts by ginsenosides. Phytotherapy Research, 1, 58–60.
14.
RussellTR. (1981) [60] Growth and cytodifferentiation of 3T3-L1 preadipocytes into adipocytes. Methods in Enzymology, 72, 720–723.
15.
GreenH, KehindeO. (1975) An established preadipose cell line and its differentiation in culture II. Factors affecting the adipose conversion. Cell, 5, 19–27.
16.
RubinCS, HirschA, FungC, RosenOM. (1978) Development of hormone receptors and hormonal responsiveness in vitro. Insulin receptors and insulin sensitivity in the preadipocyte and adipocyte forms of 3T3-L1 cells. Journal of Biological Chemistry, 253, 7570–7578.